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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Role of Angiopoietin-2 in Corneal Lymphangiogenesis</title><author><name sortKey="Yuen, Don" sort="Yuen, Don" uniqKey="Yuen D" first="Don" last="Yuen">Don Yuen</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Grimaldo, Sammy" sort="Grimaldo, Sammy" uniqKey="Grimaldo S" first="Sammy" last="Grimaldo">Sammy Grimaldo</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Sessa, Roberto" sort="Sessa, Roberto" uniqKey="Sessa R" first="Roberto" last="Sessa">Roberto Sessa</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Ecoiffier, Tatiana" sort="Ecoiffier, Tatiana" uniqKey="Ecoiffier T" first="Tatiana" last="Ecoiffier">Tatiana Ecoiffier</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Truong, Tan" sort="Truong, Tan" uniqKey="Truong T" first="Tan" last="Truong">Tan Truong</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Graduate Group in Vision Science, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Huang, Eric" sort="Huang, Eric" uniqKey="Huang E" first="Eric" last="Huang">Eric Huang</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Bernas, Michael" sort="Bernas, Michael" uniqKey="Bernas M" first="Michael" last="Bernas">Michael Bernas</name><affiliation><nlm:aff id="aff3">Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona, United States</nlm:aff></affiliation></author><author><name sortKey="Daley, Sarah" sort="Daley, Sarah" uniqKey="Daley S" first="Sarah" last="Daley">Sarah Daley</name><affiliation><nlm:aff id="aff3">Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona, United States</nlm:aff></affiliation></author><author><name sortKey="Witte, Marlys" sort="Witte, Marlys" uniqKey="Witte M" first="Marlys" last="Witte">Marlys Witte</name><affiliation><nlm:aff id="aff3">Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona, United States</nlm:aff></affiliation></author><author><name sortKey="Chen, Lu" sort="Chen, Lu" uniqKey="Chen L" first="Lu" last="Chen">Lu Chen</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Graduate Group in Vision Science, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24781940</idno><idno type="pmc">4039380</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4039380</idno><idno type="RBID">PMC:4039380</idno><idno type="doi">10.1167/iovs.13-13779</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">002E71</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002E71</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Role of Angiopoietin-2 in Corneal Lymphangiogenesis</title><author><name sortKey="Yuen, Don" sort="Yuen, Don" uniqKey="Yuen D" first="Don" last="Yuen">Don Yuen</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Grimaldo, Sammy" sort="Grimaldo, Sammy" uniqKey="Grimaldo S" first="Sammy" last="Grimaldo">Sammy Grimaldo</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Sessa, Roberto" sort="Sessa, Roberto" uniqKey="Sessa R" first="Roberto" last="Sessa">Roberto Sessa</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Ecoiffier, Tatiana" sort="Ecoiffier, Tatiana" uniqKey="Ecoiffier T" first="Tatiana" last="Ecoiffier">Tatiana Ecoiffier</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Truong, Tan" sort="Truong, Tan" uniqKey="Truong T" first="Tan" last="Truong">Tan Truong</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Graduate Group in Vision Science, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Huang, Eric" sort="Huang, Eric" uniqKey="Huang E" first="Eric" last="Huang">Eric Huang</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author><author><name sortKey="Bernas, Michael" sort="Bernas, Michael" uniqKey="Bernas M" first="Michael" last="Bernas">Michael Bernas</name><affiliation><nlm:aff id="aff3">Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona, United States</nlm:aff></affiliation></author><author><name sortKey="Daley, Sarah" sort="Daley, Sarah" uniqKey="Daley S" first="Sarah" last="Daley">Sarah Daley</name><affiliation><nlm:aff id="aff3">Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona, United States</nlm:aff></affiliation></author><author><name sortKey="Witte, Marlys" sort="Witte, Marlys" uniqKey="Witte M" first="Marlys" last="Witte">Marlys Witte</name><affiliation><nlm:aff id="aff3">Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona, United States</nlm:aff></affiliation></author><author><name sortKey="Chen, Lu" sort="Chen, Lu" uniqKey="Chen L" first="Lu" last="Chen">Lu Chen</name><affiliation><nlm:aff id="aff1">Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation><affiliation><nlm:aff id="aff2">Graduate Group in Vision Science, University of California, Berkeley, Berkeley, California, United States</nlm:aff></affiliation></author></analytic><series><title level="j">Investigative Ophthalmology & Visual Science</title><idno type="ISSN">0146-0404</idno><idno type="eISSN">1552-5783</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Purpose.</title><p>Lymphatic research has progressed rapidly in recent years. Lymphatic dysfunction has been found in myriad disorders from cancer metastasis to transplant rejection; however, effective treatment for lymphatic disorders is still limited. This study investigates the role of angiopoietin-2 (Ang-2) in corneal inflammatory lymphangiogenesis (LG) in vivo and in lymphatic endothelial cell (LEC) functions in vitro.</p></sec><sec id="st2"><title>Methods.</title><p>Standard suture placement model was used to study Ang-2 expression in inflamed cornea, and corneal LG and hemangiogenesis (HG) responses in Ang-2 knockout mice. Moreover, human LEC culture system was used to examine the effect of Ang-2 gene knockdown on LEC functions using small interfering RNAs (siRNAs). The effect of siRNA treatment on corneal LG was also assessed in vivo.</p></sec><sec id="st3"><title>Results.</title><p>Angiopoietin-2 was expressed on lymphatic vessels and macrophages in inflamed cornea. While corneal LG response was abolished in Ang-2 knockout mice, the HG response was also significantly suppressed with disorganized patterning. Moreover, anti-Ang-2 treatment inhibited LEC proliferation and capillary tube formation in vitro and corneal LG in vivo.</p></sec><sec id="st4"><title>Conclusions.</title><p>Angiopoietin-2 is critically involved in lymphatic processes in vivo and in vitro. Further investigation of the Ang-2 pathway may provide novel insights and therapeutic strategies for lymphatic-related disorders, which occur both inside and outside the eye.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Invest Ophthalmol Vis Sci</journal-id><journal-id journal-id-type="iso-abbrev">Invest. Ophthalmol. Vis. Sci</journal-id><journal-id journal-id-type="hwp">iovs</journal-id><journal-id journal-id-type="pmc">iovs</journal-id><journal-id journal-id-type="publisher-id">IOVS</journal-id><journal-title-group><journal-title>Investigative Ophthalmology & Visual Science</journal-title></journal-title-group><issn pub-type="ppub">0146-0404</issn><issn pub-type="epub">1552-5783</issn><publisher><publisher-name>The Association for Research in Vision and Ophthalmology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24781940</article-id><article-id pub-id-type="pmc">4039380</article-id><article-id pub-id-type="doi">10.1167/iovs.13-13779</article-id><article-id pub-id-type="sici">iovs-55-04-90</article-id><article-id pub-id-type="other">IOVS-13-13779</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject><subj-group><subject>Cornea</subject></subj-group></subj-group></article-categories><title-group><article-title>Role of Angiopoietin-2 in Corneal Lymphangiogenesis</article-title><alt-title alt-title-type="runhead">Ang-2 in Lymphangiogenesis</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Yuen</surname><given-names>Don</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Grimaldo</surname><given-names>Sammy</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sessa</surname><given-names>Roberto</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Ecoiffier</surname><given-names>Tatiana</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Truong</surname><given-names>Tan</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Eric</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Bernas</surname><given-names>Michael</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Daley</surname><given-names>Sarah</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Witte</surname><given-names>Marlys</given-names></name><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Lu</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label>Center for Eye Disease and Development, Program in Vision Science and School of Optometry, University of California, Berkeley, Berkeley, California, United States</aff><aff id="aff2"><label>2</label>Graduate Group in Vision Science, University of California, Berkeley, Berkeley, California, United States</aff><aff id="aff3"><label>3</label>Department of Surgery, School of Medicine, University of Arizona, Tucson, Arizona, United States</aff><author-notes><corresp id="cor1">Correspondence: Lu Chen, 689 Minor Hall, University of California, Berkeley, CA 94720, USA; <email>chenlu@berkeley.edu</email>.</corresp></author-notes><pub-date pub-type="epub"><day>29</day><month>5</month><year>2014</year></pub-date><pub-date pub-type="ppub"><month>5</month><year>2014</year></pub-date><volume>55</volume><issue>5</issue><fpage>3320</fpage><lpage>3327</lpage><history><date date-type="received"><day>16</day><month>12</month><year>2013</year></date><date date-type="accepted"><day>21</day><month>4</month><year>2014</year></date></history><permissions><copyright-statement>Copyright 2014 The Association for Research in Vision and Ophthalmology, Inc.</copyright-statement><copyright-year>2014</copyright-year></permissions><abstract><sec id="st1"><title>Purpose.</title><p>Lymphatic research has progressed rapidly in recent years. Lymphatic dysfunction has been found in myriad disorders from cancer metastasis to transplant rejection; however, effective treatment for lymphatic disorders is still limited. This study investigates the role of angiopoietin-2 (Ang-2) in corneal inflammatory lymphangiogenesis (LG) in vivo and in lymphatic endothelial cell (LEC) functions in vitro.</p></sec><sec id="st2"><title>Methods.</title><p>Standard suture placement model was used to study Ang-2 expression in inflamed cornea, and corneal LG and hemangiogenesis (HG) responses in Ang-2 knockout mice. Moreover, human LEC culture system was used to examine the effect of Ang-2 gene knockdown on LEC functions using small interfering RNAs (siRNAs). The effect of siRNA treatment on corneal LG was also assessed in vivo.</p></sec><sec id="st3"><title>Results.</title><p>Angiopoietin-2 was expressed on lymphatic vessels and macrophages in inflamed cornea. While corneal LG response was abolished in Ang-2 knockout mice, the HG response was also significantly suppressed with disorganized patterning. Moreover, anti-Ang-2 treatment inhibited LEC proliferation and capillary tube formation in vitro and corneal LG in vivo.</p></sec><sec id="st4"><title>Conclusions.</title><p>Angiopoietin-2 is critically involved in lymphatic processes in vivo and in vitro. Further investigation of the Ang-2 pathway may provide novel insights and therapeutic strategies for lymphatic-related disorders, which occur both inside and outside the eye.</p></sec></abstract><abstract abstract-type="precis"><p>This study reveals Ang-2 gene deficiency leads to marked suppression of lymphangiogenesis in vivo and lymphatic endothelial cell functions in intro. Future investigation on Ang-2 pathway may provide novel therapeutic strategies for lymphatic-related diseases.</p></abstract><kwd-group><title>Keywords</title><kwd>corneal lymphangiogenesis</kwd><kwd>Ang-2</kwd><kwd>knockout mice</kwd><kwd>small interfering RNA</kwd><kwd>lymphatic endothelial cells</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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