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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Validation of the cantharidin-induced skin blister as an <italic>in vivo</italic> model of inflammation</title><author><name sortKey="Dinh, Phong Huy Duc" sort="Dinh, Phong Huy Duc" uniqKey="Dinh P" first="Phong Huy Duc" last="Dinh">Phong Huy Duc Dinh</name><affiliation><nlm:aff id="au1"><institution>Clinics of Immuno-allergology</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au3"><institution>Department of Immunology, Pham Ngoc Thach University of Medicine (PNTU)</institution><addr-line>Ho Chi Minh City, Vietnam</addr-line></nlm:aff></affiliation></author><author><name sortKey="Corraza, Francis" sort="Corraza, Francis" uniqKey="Corraza F" first="Francis" last="Corraza">Francis Corraza</name><affiliation><nlm:aff id="au2"><institution>Hematology, CHU Brugmann (Université Libre de Bruxelles – ULB)</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation></author><author><name sortKey="Mestdagh, Kristel" sort="Mestdagh, Kristel" uniqKey="Mestdagh K" first="Kristel" last="Mestdagh">Kristel Mestdagh</name><affiliation><nlm:aff id="au1"><institution>Clinics of Immuno-allergology</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kassengera, Zaina" sort="Kassengera, Zaina" uniqKey="Kassengera Z" first="Zaina" last="Kassengera">Zaina Kassengera</name><affiliation><nlm:aff id="au2"><institution>Hematology, CHU Brugmann (Université Libre de Bruxelles – ULB)</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation></author><author><name sortKey="Doyen, Virginie" sort="Doyen, Virginie" uniqKey="Doyen V" first="Virginie" last="Doyen">Virginie Doyen</name><affiliation><nlm:aff id="au1"><institution>Clinics of Immuno-allergology</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation></author><author><name sortKey="Michel, Olivier" sort="Michel, Olivier" uniqKey="Michel O" first="Olivier" last="Michel">Olivier Michel</name><affiliation><nlm:aff id="au1"><institution>Clinics of Immuno-allergology</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21595743</idno><idno type="pmc">3244638</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3244638</idno><idno type="RBID">PMC:3244638</idno><idno type="doi">10.1111/j.1365-2125.2011.04020.x</idno><date when="2011">2011</date><idno type="wicri:Area/Pmc/Corpus">002C89</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002C89</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Validation of the cantharidin-induced skin blister as an <italic>in vivo</italic> model of inflammation</title><author><name sortKey="Dinh, Phong Huy Duc" sort="Dinh, Phong Huy Duc" uniqKey="Dinh P" first="Phong Huy Duc" last="Dinh">Phong Huy Duc Dinh</name><affiliation><nlm:aff id="au1"><institution>Clinics of Immuno-allergology</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation><affiliation><nlm:aff id="au3"><institution>Department of Immunology, Pham Ngoc Thach University of Medicine (PNTU)</institution><addr-line>Ho Chi Minh City, Vietnam</addr-line></nlm:aff></affiliation></author><author><name sortKey="Corraza, Francis" sort="Corraza, Francis" uniqKey="Corraza F" first="Francis" last="Corraza">Francis Corraza</name><affiliation><nlm:aff id="au2"><institution>Hematology, CHU Brugmann (Université Libre de Bruxelles – ULB)</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation></author><author><name sortKey="Mestdagh, Kristel" sort="Mestdagh, Kristel" uniqKey="Mestdagh K" first="Kristel" last="Mestdagh">Kristel Mestdagh</name><affiliation><nlm:aff id="au1"><institution>Clinics of Immuno-allergology</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation></author><author><name sortKey="Kassengera, Zaina" sort="Kassengera, Zaina" uniqKey="Kassengera Z" first="Zaina" last="Kassengera">Zaina Kassengera</name><affiliation><nlm:aff id="au2"><institution>Hematology, CHU Brugmann (Université Libre de Bruxelles – ULB)</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation></author><author><name sortKey="Doyen, Virginie" sort="Doyen, Virginie" uniqKey="Doyen V" first="Virginie" last="Doyen">Virginie Doyen</name><affiliation><nlm:aff id="au1"><institution>Clinics of Immuno-allergology</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation></author><author><name sortKey="Michel, Olivier" sort="Michel, Olivier" uniqKey="Michel O" first="Olivier" last="Michel">Olivier Michel</name><affiliation><nlm:aff id="au1"><institution>Clinics of Immuno-allergology</institution><addr-line>Brussels, Belgium</addr-line></nlm:aff></affiliation></author></analytic><series><title level="j">British Journal of Clinical Pharmacology</title><idno type="ISSN">0306-5251</idno><idno type="eISSN">1365-2125</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>AIM</title><p>Pharmacological profiling techniques, such as the cantharidin-induced skin blister, may be used to assess the anti-inflammatory properties of novel drugs. However, no data are available on the reproducibility of this technique or on the blocking effect of anti-inflammatory drugs, such as anti-TNF and corticosteroids.</p></sec><sec><title>METHODS</title><p>A group of 30 healthy subjects were randomized into three parallel groups treated with placebo, oral methylprednisolone 20 mg day<sup>−1</sup> for 7 days or anti-tumour necrosis factor (TNF) (adalimumab, Humira®, Abbott) 40 mg s.c. single dose. A first blister was induced at baseline and collected, immediately before the start of treatment and a second blister was obtained 7 days after the start of treatment. The total number of cells, the cell viability and the differential cell count were evaluated by two independent observers, who were blind to treatment. <sc>anova</sc> was used to compare change from baseline among the three groups before pairwise comparisons.</p></sec><sec><title>RESULTS</title><p>Among the placebo group, there was no significant difference in the total cell count, neutrophils, eosinophils and monocytes between day 1 and day 7. Methylprednisolone inhibited the eosinophil influx in mean % (95% CI) (−1.0 (−1.7, −0.3); <italic>P</italic> < 0.02) and absolute (<italic>P</italic> < 0.02) values, while anti-TNF inhibited the neutrophil influx in mean % (95% CI) (−19.3 (−29.5, −9.1); <italic>P</italic> < 0.01) and absolute (<italic>P</italic> < 0.05) values.</p></sec><sec><title>CONCLUSIONS</title><p>The cantharidin-induced skin blister is a safe, well tolerated and reproducible procedure. Pre-treatment with anti-TNF or methylprednisolone inhibited the neutrophilic or eosinophilic trafficking, respectively. It could be useful in profiling anti-inflammatory drugs regarding their effects on the cellular inflammatory response.</p></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Br J Clin Pharmacol</journal-id><journal-id journal-id-type="publisher-id">bcp</journal-id><journal-title-group><journal-title>British Journal of Clinical Pharmacology</journal-title></journal-title-group><issn pub-type="ppub">0306-5251</issn><issn pub-type="epub">1365-2125</issn><publisher><publisher-name>Blackwell Science Inc</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">21595743</article-id><article-id pub-id-type="pmc">3244638</article-id><article-id pub-id-type="doi">10.1111/j.1365-2125.2011.04020.x</article-id><article-categories><subj-group subj-group-type="heading"><subject>Clinical Trials</subject></subj-group></article-categories><title-group><article-title>Validation of the cantharidin-induced skin blister as an <italic>in vivo</italic> model of inflammation</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dinh</surname><given-names>Phong Huy Duc</given-names></name><xref ref-type="aff" rid="au1">1</xref><xref ref-type="aff" rid="au3">3</xref></contrib><contrib contrib-type="author"><name><surname>Corraza</surname><given-names>Francis</given-names></name><xref ref-type="aff" rid="au2">2</xref></contrib><contrib contrib-type="author"><name><surname>Mestdagh</surname><given-names>Kristel</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kassengera</surname><given-names>Zaina</given-names></name><xref ref-type="aff" rid="au2">2</xref></contrib><contrib contrib-type="author"><name><surname>Doyen</surname><given-names>Virginie</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><contrib contrib-type="author"><name><surname>Michel</surname><given-names>Olivier</given-names></name><xref ref-type="aff" rid="au1">1</xref></contrib><aff id="au1"><label>1</label><institution>Clinics of Immuno-allergology</institution><addr-line>Brussels, Belgium</addr-line></aff><aff id="au2"><label>2</label><institution>Hematology, CHU Brugmann (Université Libre de Bruxelles – ULB)</institution><addr-line>Brussels, Belgium</addr-line></aff><aff id="au3"><label>3</label><institution>Department of Immunology, Pham Ngoc Thach University of Medicine (PNTU)</institution><addr-line>Ho Chi Minh City, Vietnam</addr-line></aff></contrib-group><author-notes><corresp id="cor1">Dr Olivier Michel MD PhD, CHU Brugmann/ULB – Université Libre de Bruxelles, place Van Gehuchten, 4, B −1020 Brussels, Belgium. Tel.: +32 2 477 2272, Fax: +32 2 477 2276, E-mail: <email>omichel@ulb.ac.be</email></corresp></author-notes><pub-date pub-type="ppub"><month>12</month><year>2011</year></pub-date><volume>72</volume><issue>6</issue><fpage>912</fpage><lpage>920</lpage><history><date date-type="received"><day>22</day><month>11</month><year>2010</year></date><date date-type="accepted"><day>13</day><month>5</month><year>2011</year></date><date date-type="accepted"><day>19</day><month>5</month><year>2011</year></date></history><permissions><copyright-statement>Copyright © 2011 The British Pharmacological Society</copyright-statement><copyright-year>2011</copyright-year></permissions><abstract><sec><title>AIM</title><p>Pharmacological profiling techniques, such as the cantharidin-induced skin blister, may be used to assess the anti-inflammatory properties of novel drugs. However, no data are available on the reproducibility of this technique or on the blocking effect of anti-inflammatory drugs, such as anti-TNF and corticosteroids.</p></sec><sec><title>METHODS</title><p>A group of 30 healthy subjects were randomized into three parallel groups treated with placebo, oral methylprednisolone 20 mg day<sup>−1</sup> for 7 days or anti-tumour necrosis factor (TNF) (adalimumab, Humira®, Abbott) 40 mg s.c. single dose. A first blister was induced at baseline and collected, immediately before the start of treatment and a second blister was obtained 7 days after the start of treatment. The total number of cells, the cell viability and the differential cell count were evaluated by two independent observers, who were blind to treatment. <sc>anova</sc> was used to compare change from baseline among the three groups before pairwise comparisons.</p></sec><sec><title>RESULTS</title><p>Among the placebo group, there was no significant difference in the total cell count, neutrophils, eosinophils and monocytes between day 1 and day 7. Methylprednisolone inhibited the eosinophil influx in mean % (95% CI) (−1.0 (−1.7, −0.3); <italic>P</italic> < 0.02) and absolute (<italic>P</italic> < 0.02) values, while anti-TNF inhibited the neutrophil influx in mean % (95% CI) (−19.3 (−29.5, −9.1); <italic>P</italic> < 0.01) and absolute (<italic>P</italic> < 0.05) values.</p></sec><sec><title>CONCLUSIONS</title><p>The cantharidin-induced skin blister is a safe, well tolerated and reproducible procedure. Pre-treatment with anti-TNF or methylprednisolone inhibited the neutrophilic or eosinophilic trafficking, respectively. It could be useful in profiling anti-inflammatory drugs regarding their effects on the cellular inflammatory response.</p></sec></abstract><kwd-group><kwd>anti-TNF</kwd><kwd>blister</kwd><kwd>cantharidin</kwd><kwd>inflammation</kwd><kwd>steroids</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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