LymphedemaV1, Pmc, Corpus, bibRecord, 002A33

In vivo dynamic imaging of intestinal motions using diet-related autofluorescence

Identifieur interne : 002A33 ( Pmc/Corpus ); précédent : 002A32; suivant : 002A34

In vivo dynamic imaging of intestinal motions using diet-related autofluorescence

Auteurs : Sunkuk Kwon ; Cynthia Davies-Venn ; Eva M. Sevick-Muraca

Source :

Neurogastroenterology and Motility [ 1350-1925 ] ; 2012.

RBID : PMC:3324656

Abstract

Background

Tissue background autofluorescence induced by standard murine diets containing chlorophyll is a significant problem for fluorescence whole-body imaging. However, since red chlorophyll autofluorescence delineates the gastrointestinal (GI) tract in the abdomen of the mouse, it should be possible to dynamically and non-invasively image intestinal motions. Herein, we non-invasively imaged for the first time intestinal motions, such as peristaltic and segmental motions, without an exogenous imaging agent, using red chlorophyll fluorescence.

Methods

Mice were illuminated with 660 nm light from a laser diode and autofluorescence at 710 nm was acquired dynamically for 5 mins with 200 ms exposure time. Fluorescent imaging data were analyzed to generate a three dimensional spatio-temporal map to quantify intestinal motions.

Results

Peristaltic and segmental motions were observed in vivo in mice. Our quantification showed that the frequency and propagation velocity of peristaltic contractile waves in the small intestine were measured to be 28.6 cycles/min and 1.82 ± 0.56 cm/s, respectively.

Conclusions & Inferences

This simple, but unexplored imaging technique can provide a means to monitor intestinal motility disorders and response to therapeutic agents.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324656

DOI: 10.1111/j.1365-2982.2012.01886.x
PubMed: 22309466
PubMed Central: 3324656

Links to Exploration step

PMC:3324656

Le document en format XML

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 dynamic imaging of intestinal motions using diet-related autofluorescence</title>
<author><name sortKey="Kwon, Sunkuk" sort="Kwon, Sunkuk" uniqKey="Kwon S" first="Sunkuk" last="Kwon">Sunkuk Kwon</name>
</author>
<author><name sortKey="Davies Venn, Cynthia" sort="Davies Venn, Cynthia" uniqKey="Davies Venn C" first="Cynthia" last="Davies-Venn">Cynthia Davies-Venn</name>
</author>
<author><name sortKey="Sevick Muraca, Eva M" sort="Sevick Muraca, Eva M" uniqKey="Sevick Muraca E" first="Eva M" last="Sevick-Muraca">Eva M. Sevick-Muraca</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In vivo</italic>
 dynamic imaging of intestinal motions using diet-related autofluorescence</title>
<author><name sortKey="Kwon, Sunkuk" sort="Kwon, Sunkuk" uniqKey="Kwon S" first="Sunkuk" last="Kwon">Sunkuk Kwon</name>
</author>
<author><name sortKey="Davies Venn, Cynthia" sort="Davies Venn, Cynthia" uniqKey="Davies Venn C" first="Cynthia" last="Davies-Venn">Cynthia Davies-Venn</name>
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<author><name sortKey="Sevick Muraca, Eva M" sort="Sevick Muraca, Eva M" uniqKey="Sevick Muraca E" first="Eva M" last="Sevick-Muraca">Eva M. Sevick-Muraca</name>
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<series><title level="j">Neurogastroenterology and Motility</title>
<idno type="ISSN">1350-1925</idno>
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<imprint><date when="2012">2012</date>
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<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Background</title>
<p id="P1">Tissue background autofluorescence induced by standard murine diets containing chlorophyll is a significant problem for fluorescence whole-body imaging. However, since red chlorophyll autofluorescence delineates the gastrointestinal (GI) tract in the abdomen of the mouse, it should be possible to dynamically and non-invasively image intestinal motions. Herein, we non-invasively imaged for the first time intestinal motions, such as peristaltic and segmental motions, without an exogenous imaging agent, using red chlorophyll fluorescence.</p>
</sec>
<sec sec-type="methods" id="S2"><title>Methods</title>
<p id="P2">Mice were illuminated with 660 nm light from a laser diode and autofluorescence at 710 nm was acquired dynamically for 5 mins with 200 ms exposure time. Fluorescent imaging data were analyzed to generate a three dimensional spatio-temporal map to quantify intestinal motions.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Peristaltic and segmental motions were observed <italic>in vivo</italic>
 in mice. Our quantification showed that the frequency and propagation velocity of peristaltic contractile waves in the small intestine were measured to be 28.6 cycles/min and 1.82 ± 0.56 cm/s, respectively.</p>
</sec>
<sec id="S4"><title>Conclusions & Inferences</title>
<p id="P4">This simple, but unexplored imaging technique can provide a means to monitor intestinal motility disorders and response to therapeutic agents.</p>
</sec>
</div>
</front>
</TEI>
<pmc article-type="research-article" xml:lang="en"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <pmc-dir>properties manuscript</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-journal-id">9432572</journal-id>
<journal-id journal-id-type="pubmed-jr-id">20191</journal-id>
<journal-id journal-id-type="nlm-ta">Neurogastroenterol Motil</journal-id>
<journal-id journal-id-type="iso-abbrev">Neurogastroenterol. Motil.</journal-id>
<journal-title-group><journal-title>Neurogastroenterology and Motility</journal-title>
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<issn pub-type="ppub">1350-1925</issn>
<issn pub-type="epub">1365-2982</issn>
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<article-meta><article-id pub-id-type="pmid">22309466</article-id>
<article-id pub-id-type="pmc">3324656</article-id>
<article-id pub-id-type="doi">10.1111/j.1365-2982.2012.01886.x</article-id>
<article-id pub-id-type="manuscript">NIHMS350156</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
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</article-categories>
<title-group><article-title><italic>In vivo</italic>
 dynamic imaging of intestinal motions using diet-related autofluorescence</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Kwon</surname>
<given-names>Sunkuk</given-names>
</name>
<xref ref-type="corresp" rid="cor1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Davies-Venn</surname>
<given-names>Cynthia</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Sevick-Muraca</surname>
<given-names>Eva M</given-names>
</name>
</contrib>
<aff id="A1">Center for Molecular Imaging, The Brown Foundation Institute of Molecular Medicine, The University of Texas Health Science Center, Houston, TX, 77030</aff>
</contrib-group>
<author-notes><corresp id="cor1"><label>1</label>
To whom correspondence should be directed, Center for Molecular Imaging, The Brown Foundation of Molecular Medicine, The University of Texas Health Science Center, 1825 Pressler Street, SRB 330F, Houston, Texas 77030; phone: 713-500-3393; fax: 713-500-0319; <email>sunkuk.kwon@uth.tmc.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>4</day>
<month>2</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="epub"><day>6</day>
<month>2</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="ppub"><month>5</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>1</day>
<month>5</month>
<year>2013</year>
</pub-date>
<volume>24</volume>
<issue>5</issue>
<fpage>494</fpage>
<lpage>497</lpage>
<abstract><sec id="S1"><title>Background</title>
<p id="P1">Tissue background autofluorescence induced by standard murine diets containing chlorophyll is a significant problem for fluorescence whole-body imaging. However, since red chlorophyll autofluorescence delineates the gastrointestinal (GI) tract in the abdomen of the mouse, it should be possible to dynamically and non-invasively image intestinal motions. Herein, we non-invasively imaged for the first time intestinal motions, such as peristaltic and segmental motions, without an exogenous imaging agent, using red chlorophyll fluorescence.</p>
</sec>
<sec sec-type="methods" id="S2"><title>Methods</title>
<p id="P2">Mice were illuminated with 660 nm light from a laser diode and autofluorescence at 710 nm was acquired dynamically for 5 mins with 200 ms exposure time. Fluorescent imaging data were analyzed to generate a three dimensional spatio-temporal map to quantify intestinal motions.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P3">Peristaltic and segmental motions were observed <italic>in vivo</italic>
 in mice. Our quantification showed that the frequency and propagation velocity of peristaltic contractile waves in the small intestine were measured to be 28.6 cycles/min and 1.82 ± 0.56 cm/s, respectively.</p>
</sec>
<sec id="S4"><title>Conclusions & Inferences</title>
<p id="P4">This simple, but unexplored imaging technique can provide a means to monitor intestinal motility disorders and response to therapeutic agents.</p>
</sec>
</abstract>
<kwd-group><kwd>autofluorescence</kwd>
<kwd>fluorescence imaging</kwd>
<kwd>intestinal motion</kwd>
<kwd>chlorophyll</kwd>
</kwd-group>
<funding-group><award-group><funding-source country="United States">National Institute of Diabetes and Digestive and Kidney Diseases : NIDDK</funding-source>
<award-id>P30 DK056338-10 || DK</award-id>
</award-group>
</funding-group>
</article-meta>
</front>
</pmc>
</record>

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In vivo dynamic imaging of intestinal motions using diet-related autofluorescence

In vivo dynamic imaging of intestinal motions using diet-related autofluorescence

Source :

Abstract

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