LymphedemaV1, Pmc, Corpus, bibRecord, 002763

Brugia malayi: Comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model

Identifieur interne : 002763 ( Pmc/Corpus ); précédent : 002762; suivant : 002764

Brugia malayi: Comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model

Auteurs : Sivasakthivel Thirugnanam ; Pandurangan Pandiaraja ; Kalyanasundaram Ramaswamy ; Vadivel Murugan ; Munirathinam Gnanasekar ; Krithika Nandakumar ; Maryada Venkata Rami Reddy ; Perumal Kaliraj

Source :

Experimental parasitology [ 0014-4894 ] ; 2007.

RBID : PMC:2763209

Abstract

Immunization of jirds with Bm-alt-2 elicited partial protection against challenge infection with the filarial parasite Brugia malayi. In this study, we initially compared the protective immune responses elicited following immunization with recombinant Bm-ALT-2 protein regimen and Bm-alt-2 DNA regimen. These studies showed that protein vaccination conferred approximately 75% protection compared to DNA vaccination that conferred only 57% protection. Analysis of the protective immune responses showed that the protein immunization promoted a Th2-biased response with an increase in IL-4, IL-5 and IgG1 responses, whereas, the DNA vaccine promoted a Th1-biased response with profound IFN-γ and IgG2a responses. Since protein vaccination gave better results than DNA vaccination, we then wanted to evaluate whether a prime-boost vaccination that combined DNA prime and protein boost will significantly increase the protective responses induced by the protein vaccine. Our results suggest that prime-boost vaccination had no added advantage and was comparatively less effective (64% protection) than the Bm-ALT-2 protein alone vaccination. Prime boost vaccination generated mixed Th1/Th2 responses with a slightly diminished Th2 responses compared to protein vaccination. Thus, our results suggest that Bm-ALT-2 protein vaccination regimen may be slightly better than prime-boost vaccine regimen and the mechanism of protection appears to be largely mediated by a Th2-biased response.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2763209

DOI: 10.1016/j.exppara.2007.02.017
PubMed: 17442307
PubMed Central: 2763209

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PMC:2763209

Le document en format XML

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: Comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model</title>
<author><name sortKey="Thirugnanam, Sivasakthivel" sort="Thirugnanam, Sivasakthivel" uniqKey="Thirugnanam S" first="Sivasakthivel" last="Thirugnanam">Sivasakthivel Thirugnanam</name>
<affiliation><nlm:aff id="A1"> Centre for Biotechnology, Anna University, Chennai 600 025, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Pandiaraja, Pandurangan" sort="Pandiaraja, Pandurangan" uniqKey="Pandiaraja P" first="Pandurangan" last="Pandiaraja">Pandurangan Pandiaraja</name>
<affiliation><nlm:aff id="A1"> Centre for Biotechnology, Anna University, Chennai 600 025, India</nlm:aff>
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<author><name sortKey="Ramaswamy, Kalyanasundaram" sort="Ramaswamy, Kalyanasundaram" uniqKey="Ramaswamy K" first="Kalyanasundaram" last="Ramaswamy">Kalyanasundaram Ramaswamy</name>
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<author><name sortKey="Murugan, Vadivel" sort="Murugan, Vadivel" uniqKey="Murugan V" first="Vadivel" last="Murugan">Vadivel Murugan</name>
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<author><name sortKey="Gnanasekar, Munirathinam" sort="Gnanasekar, Munirathinam" uniqKey="Gnanasekar M" first="Munirathinam" last="Gnanasekar">Munirathinam Gnanasekar</name>
<affiliation><nlm:aff id="A2"> Department of Biomedical Sciences, University of Illinois, College of Medicine, Rockford, IL 61107, USA</nlm:aff>
</affiliation>
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<author><name sortKey="Nandakumar, Krithika" sort="Nandakumar, Krithika" uniqKey="Nandakumar K" first="Krithika" last="Nandakumar">Krithika Nandakumar</name>
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<author><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name>
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<author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name>
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: Comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model</title>
<author><name sortKey="Thirugnanam, Sivasakthivel" sort="Thirugnanam, Sivasakthivel" uniqKey="Thirugnanam S" first="Sivasakthivel" last="Thirugnanam">Sivasakthivel Thirugnanam</name>
<affiliation><nlm:aff id="A1"> Centre for Biotechnology, Anna University, Chennai 600 025, India</nlm:aff>
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<author><name sortKey="Pandiaraja, Pandurangan" sort="Pandiaraja, Pandurangan" uniqKey="Pandiaraja P" first="Pandurangan" last="Pandiaraja">Pandurangan Pandiaraja</name>
<affiliation><nlm:aff id="A1"> Centre for Biotechnology, Anna University, Chennai 600 025, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ramaswamy, Kalyanasundaram" sort="Ramaswamy, Kalyanasundaram" uniqKey="Ramaswamy K" first="Kalyanasundaram" last="Ramaswamy">Kalyanasundaram Ramaswamy</name>
<affiliation><nlm:aff id="A2"> Department of Biomedical Sciences, University of Illinois, College of Medicine, Rockford, IL 61107, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Murugan, Vadivel" sort="Murugan, Vadivel" uniqKey="Murugan V" first="Vadivel" last="Murugan">Vadivel Murugan</name>
<affiliation><nlm:aff id="A1"> Centre for Biotechnology, Anna University, Chennai 600 025, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Gnanasekar, Munirathinam" sort="Gnanasekar, Munirathinam" uniqKey="Gnanasekar M" first="Munirathinam" last="Gnanasekar">Munirathinam Gnanasekar</name>
<affiliation><nlm:aff id="A2"> Department of Biomedical Sciences, University of Illinois, College of Medicine, Rockford, IL 61107, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Nandakumar, Krithika" sort="Nandakumar, Krithika" uniqKey="Nandakumar K" first="Krithika" last="Nandakumar">Krithika Nandakumar</name>
<affiliation><nlm:aff id="A3"> Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Reddy, Maryada Venkata Rami" sort="Reddy, Maryada Venkata Rami" uniqKey="Reddy M" first="Maryada Venkata Rami" last="Reddy">Maryada Venkata Rami Reddy</name>
<affiliation><nlm:aff id="A3"> Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name>
<affiliation><nlm:aff id="A1"> Centre for Biotechnology, Anna University, Chennai 600 025, India</nlm:aff>
</affiliation>
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<series><title level="j">Experimental parasitology</title>
<idno type="ISSN">0014-4894</idno>
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<imprint><date when="2007">2007</date>
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<front><div type="abstract" xml:lang="en"><p id="P1">Immunization of jirds with Bm-alt-2 elicited partial protection against challenge infection with the filarial parasite <italic>Brugia malayi</italic>
. In this study, we initially compared the protective immune responses elicited following immunization with recombinant Bm-ALT-2 protein regimen and Bm-alt-2 DNA regimen. These studies showed that protein vaccination conferred approximately 75% protection compared to DNA vaccination that conferred only 57% protection. Analysis of the protective immune responses showed that the protein immunization promoted a Th2-biased response with an increase in IL-4, IL-5 and IgG1 responses, whereas, the DNA vaccine promoted a Th1-biased response with profound IFN-γ and IgG2a responses. Since protein vaccination gave better results than DNA vaccination, we then wanted to evaluate whether a prime-boost vaccination that combined DNA prime and protein boost will significantly increase the protective responses induced by the protein vaccine. Our results suggest that prime-boost vaccination had no added advantage and was comparatively less effective (64% protection) than the Bm-ALT-2 protein alone vaccination. Prime boost vaccination generated mixed Th1/Th2 responses with a slightly diminished Th2 responses compared to protein vaccination. Thus, our results suggest that Bm-ALT-2 protein vaccination regimen may be slightly better than prime-boost vaccine regimen and the mechanism of protection appears to be largely mediated by a Th2-biased response.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <pmc-dir>properties manuscript</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-journal-id">0370713</journal-id>
<journal-id journal-id-type="pubmed-jr-id">3662</journal-id>
<journal-id journal-id-type="nlm-ta">Exp Parasitol</journal-id>
<journal-title>Experimental parasitology</journal-title>
<issn pub-type="ppub">0014-4894</issn>
<issn pub-type="epub">1090-2449</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">17442307</article-id>
<article-id pub-id-type="pmc">2763209</article-id>
<article-id pub-id-type="doi">10.1016/j.exppara.2007.02.017</article-id>
<article-id pub-id-type="manuscript">NIHMS126827</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title><italic>Brugia malayi</italic>
: Comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Thirugnanam</surname>
<given-names>Sivasakthivel</given-names>
</name>
<xref rid="A1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pandiaraja</surname>
<given-names>Pandurangan</given-names>
</name>
<xref rid="A1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ramaswamy</surname>
<given-names>Kalyanasundaram</given-names>
</name>
<xref rid="A2" ref-type="aff">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Murugan</surname>
<given-names>Vadivel</given-names>
</name>
<xref rid="A1" ref-type="aff">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Gnanasekar</surname>
<given-names>Munirathinam</given-names>
</name>
<xref rid="A2" ref-type="aff">b</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Nandakumar</surname>
<given-names>Krithika</given-names>
</name>
<xref rid="A3" ref-type="aff">c</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Reddy</surname>
<given-names>Maryada Venkata Rami</given-names>
</name>
<xref rid="A3" ref-type="aff">c</xref>
<xref rid="FN1" ref-type="author-notes">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kaliraj</surname>
<given-names>Perumal</given-names>
</name>
<xref rid="A1" ref-type="aff">a</xref>
<xref rid="FN1" ref-type="author-notes">*</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>a</label>
 Centre for Biotechnology, Anna University, Chennai 600 025, India</aff>
<aff id="A2"><label>b</label>
 Department of Biomedical Sciences, University of Illinois, College of Medicine, Rockford, IL 61107, USA</aff>
<aff id="A3"><label>c</label>
 Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences, Sevagram, India</aff>
<author-notes><corresp id="FN1"><label>*</label>
 Corresponding authors. Fax: +91 44 22542299. <email>mvrreddy_wda@sancharnet.in</email>
 (M.V.R. Reddy), <email>pkaliraj@annauniv.edu</email>
 (P. Kaliraj)</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>28</day>
<month>9</month>
<year>2009</year>
</pub-date>
<pub-date pub-type="epub"><day>6</day>
<month>3</month>
<year>2007</year>
</pub-date>
<pub-date pub-type="ppub"><month>8</month>
<year>2007</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>19</day>
<month>10</month>
<year>2009</year>
</pub-date>
<volume>116</volume>
<issue>4</issue>
<fpage>483</fpage>
<lpage>491</lpage>
<abstract><p id="P1">Immunization of jirds with Bm-alt-2 elicited partial protection against challenge infection with the filarial parasite <italic>Brugia malayi</italic>
. In this study, we initially compared the protective immune responses elicited following immunization with recombinant Bm-ALT-2 protein regimen and Bm-alt-2 DNA regimen. These studies showed that protein vaccination conferred approximately 75% protection compared to DNA vaccination that conferred only 57% protection. Analysis of the protective immune responses showed that the protein immunization promoted a Th2-biased response with an increase in IL-4, IL-5 and IgG1 responses, whereas, the DNA vaccine promoted a Th1-biased response with profound IFN-γ and IgG2a responses. Since protein vaccination gave better results than DNA vaccination, we then wanted to evaluate whether a prime-boost vaccination that combined DNA prime and protein boost will significantly increase the protective responses induced by the protein vaccine. Our results suggest that prime-boost vaccination had no added advantage and was comparatively less effective (64% protection) than the Bm-ALT-2 protein alone vaccination. Prime boost vaccination generated mixed Th1/Th2 responses with a slightly diminished Th2 responses compared to protein vaccination. Thus, our results suggest that Bm-ALT-2 protein vaccination regimen may be slightly better than prime-boost vaccine regimen and the mechanism of protection appears to be largely mediated by a Th2-biased response.</p>
</abstract>
<kwd-group><kwd><italic>Brugia malayi</italic>
</kwd>
<kwd>Filariasis</kwd>
<kwd>Bm-alt-2</kwd>
<kwd>DNA prime-protein boost vaccination</kwd>
</kwd-group>
<contract-num rid="AI1">R56 AI064745-01A1</contract-num>
<contract-num rid="AI1">R01 AI064745-02</contract-num>
<contract-sponsor id="AI1">National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID</contract-sponsor>
</article-meta>
</front>
</pmc>
</record>

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Brugia malayi: Comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model

Brugia malayi: Comparison of protective immune responses induced by Bm-alt-2 DNA, recombinant Bm-ALT-2 protein and prime-boost vaccine regimens in a jird model

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Abstract

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