Links to Exploration step
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cloning and characterization of a potentially protective antigen in lymphatic filariasis.</title><author><name sortKey="Nilsen, T W" sort="Nilsen, T W" uniqKey="Nilsen T" first="T W" last="Nilsen">T W Nilsen</name></author><author><name sortKey="Maroney, P A" sort="Maroney, P A" uniqKey="Maroney P" first="P A" last="Maroney">P A Maroney</name></author><author><name sortKey="Goodwin, R G" sort="Goodwin, R G" uniqKey="Goodwin R" first="R G" last="Goodwin">R G Goodwin</name></author><author><name sortKey="Perrine, K G" sort="Perrine, K G" uniqKey="Perrine K" first="K G" last="Perrine">K G Perrine</name></author><author><name sortKey="Denker, J A" sort="Denker, J A" uniqKey="Denker J" first="J A" last="Denker">J A Denker</name></author><author><name sortKey="Nanduri, J" sort="Nanduri, J" uniqKey="Nanduri J" first="J" last="Nanduri">J. Nanduri</name></author><author><name sortKey="Kazura, J W" sort="Kazura, J W" uniqKey="Kazura J" first="J W" last="Kazura">J W Kazura</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">3368467</idno><idno type="pmc">280262</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC280262</idno><idno type="RBID">PMC:280262</idno><date when="1988">1988</date><idno type="wicri:Area/Pmc/Corpus">001A04</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001A04</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cloning and characterization of a potentially protective antigen in lymphatic filariasis.</title><author><name sortKey="Nilsen, T W" sort="Nilsen, T W" uniqKey="Nilsen T" first="T W" last="Nilsen">T W Nilsen</name></author><author><name sortKey="Maroney, P A" sort="Maroney, P A" uniqKey="Maroney P" first="P A" last="Maroney">P A Maroney</name></author><author><name sortKey="Goodwin, R G" sort="Goodwin, R G" uniqKey="Goodwin R" first="R G" last="Goodwin">R G Goodwin</name></author><author><name sortKey="Perrine, K G" sort="Perrine, K G" uniqKey="Perrine K" first="K G" last="Perrine">K G Perrine</name></author><author><name sortKey="Denker, J A" sort="Denker, J A" uniqKey="Denker J" first="J A" last="Denker">J A Denker</name></author><author><name sortKey="Nanduri, J" sort="Nanduri, J" uniqKey="Nanduri J" first="J" last="Nanduri">J. Nanduri</name></author><author><name sortKey="Kazura, J W" sort="Kazura, J W" uniqKey="Kazura J" first="J W" last="Kazura">J W Kazura</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="1988">1988</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>To facilitate biochemical studies of protective filarial antigens, a lambda gt11 cDNA library was constructed from Brugia malayi adult mRNA and screened with rabbit sera that recognizes a limited set of filarial antigens of approximately 25, 42, 60, and 112 kDa. Antigens of approximately equal to 25 and approximately equal to 60 kDa have been shown previously to induce enhanced clearance of microfilaremia in mice. A 154-base pair clone detected by immunological reactivity was used to isolate by hybridization a nearly full-length cDNA clone of 1.8 kilobases. Nucleotide-sequence analysis indicated that this clone was derived from a mRNA encoding a 63-kDa antigen. A fusion polypeptide containing 37 kDa of the Escherichia coli TrpE protein (anthranilate synthase) and 55 kDa of the cloned protein was recognized in immunoblot experiments with antisera raised against a partially purified preparation of the approximately equal to 60-kDa protective filarial antigen. These data relate the cloned antigen to a potentially protective antigen in lymphatic filariasis.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="pnas00262-0342-a" xlink:role="3604"></graphic></fig><fig id="F2"><graphic xlink:href="pnas00262-0345-a" xlink:role="3607"></graphic></fig></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Proc Natl Acad Sci U S A</journal-id><journal-title>Proceedings of the National Academy of Sciences of the United States of America</journal-title><issn pub-type="ppub">0027-8424</issn><issn pub-type="epub">1091-6490</issn></journal-meta><article-meta><article-id pub-id-type="pmid">3368467</article-id><article-id pub-id-type="pmc">280262</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Cloning and characterization of a potentially protective antigen in lymphatic filariasis.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Nilsen</surname><given-names>T W</given-names></name></contrib><contrib contrib-type="author"><name><surname>Maroney</surname><given-names>P A</given-names></name></contrib><contrib contrib-type="author"><name><surname>Goodwin</surname><given-names>R G</given-names></name></contrib><contrib contrib-type="author"><name><surname>Perrine</surname><given-names>K G</given-names></name></contrib><contrib contrib-type="author"><name><surname>Denker</surname><given-names>J A</given-names></name></contrib><contrib contrib-type="author"><name><surname>Nanduri</surname><given-names>J</given-names></name></contrib><contrib contrib-type="author"><name><surname>Kazura</surname><given-names>J W</given-names></name></contrib></contrib-group><aff>Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine and University Hospitals, Cleveland, OH 44106.</aff><pub-date pub-type="ppub"><month>5</month><year>1988</year></pub-date><volume>85</volume><issue>10</issue><fpage>3604</fpage><lpage>3607</lpage><abstract><p>To facilitate biochemical studies of protective filarial antigens, a lambda gt11 cDNA library was constructed from Brugia malayi adult mRNA and screened with rabbit sera that recognizes a limited set of filarial antigens of approximately 25, 42, 60, and 112 kDa. Antigens of approximately equal to 25 and approximately equal to 60 kDa have been shown previously to induce enhanced clearance of microfilaremia in mice. A 154-base pair clone detected by immunological reactivity was used to isolate by hybridization a nearly full-length cDNA clone of 1.8 kilobases. Nucleotide-sequence analysis indicated that this clone was derived from a mRNA encoding a 63-kDa antigen. A fusion polypeptide containing 37 kDa of the Escherichia coli TrpE protein (anthranilate synthase) and 55 kDa of the cloned protein was recognized in immunoblot experiments with antisera raised against a partially purified preparation of the approximately equal to 60-kDa protective filarial antigen. These data relate the cloned antigen to a potentially protective antigen in lymphatic filariasis.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><graphic xlink:href="pnas00262-0342-a" xlink:role="3604"></graphic></fig><fig id="F2"><graphic xlink:href="pnas00262-0345-a" xlink:role="3607"></graphic></fig></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001A04 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 001A04 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé=
|texte=
}}
| This area was generated with Dilib version V0.6.31. |  |