CDK19 is disrupted in a female patient with bilateral congenital retinal folds, microcephaly and mild mental retardation
Identifieur interne : 000D41 ( Pmc/Corpus ); précédent : 000D40; suivant : 000D42CDK19 is disrupted in a female patient with bilateral congenital retinal folds, microcephaly and mild mental retardation
Auteurs : Arijit Mukhopadhyay ; Jamie M. Kramer ; Gerard Merkx ; Dorien Lugtenberg ; Dominique F. Smeets ; Merel A. W. Oortveld ; Ellen A. W. Blokland ; Jyoti Agrawal ; Annette Schenck ; Hans Van Bokhoven ; Erik Huys ; Eric F. Schoenmakers ; Ad Geurts Van Kessel ; C. Erik Van Nouhuys ; Frans P. M. CremersSource :
- Human Genetics [ 0340-6717 ] ; 2010.
Abstract
Microcephaly, mental retardation and congenital retinal folds along with other systemic features have previously been reported as a separate clinical entity. The sporadic nature of the syndrome and lack of clear inheritance patterns pointed to a genetic heterogeneity. Here, we report a genetic analysis of a female patient with microcephaly, congenital bilateral falciform retinal folds, nystagmus, and mental retardation. Karyotyping revealed a de novo pericentric inversion in chromosome 6 with breakpoints in 6p12.1 and 6q21. Fluorescence in situ hybridization analysis narrowed down the region around the breakpoints, and the breakpoint at 6q21 was found to disrupt the
Url:
DOI: 10.1007/s00439-010-0848-x
PubMed: 20563892
PubMed Central: 2921488
Links to Exploration step
PMC:2921488Le document en format XML
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is disrupted in a female patient with bilateral congenital retinal folds, microcephaly and mild mental retardation</title>
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<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff2">Genomics and Molecular Medicine, Institute of Genomics and Integrative Biology (CSIR), Mall Road, Delhi, 110007 India</nlm:aff>
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<author><name sortKey="Kramer, Jamie M" sort="Kramer, Jamie M" uniqKey="Kramer J" first="Jamie M." last="Kramer">Jamie M. Kramer</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff3">Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff4">Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
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<author><name sortKey="Merkx, Gerard" sort="Merkx, Gerard" uniqKey="Merkx G" first="Gerard" last="Merkx">Gerard Merkx</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
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<author><name sortKey="Lugtenberg, Dorien" sort="Lugtenberg, Dorien" uniqKey="Lugtenberg D" first="Dorien" last="Lugtenberg">Dorien Lugtenberg</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
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<author><name sortKey="Smeets, Dominique F" sort="Smeets, Dominique F" uniqKey="Smeets D" first="Dominique F." last="Smeets">Dominique F. Smeets</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
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<author><name sortKey="Oortveld, Merel A W" sort="Oortveld, Merel A W" uniqKey="Oortveld M" first="Merel A. W." last="Oortveld">Merel A. W. Oortveld</name>
<affiliation><nlm:aff id="Aff3">Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff4">Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
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<author><name sortKey="Blokland, Ellen A W" sort="Blokland, Ellen A W" uniqKey="Blokland E" first="Ellen A. W." last="Blokland">Ellen A. W. Blokland</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
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<author><name sortKey="Agrawal, Jyoti" sort="Agrawal, Jyoti" uniqKey="Agrawal J" first="Jyoti" last="Agrawal">Jyoti Agrawal</name>
<affiliation><nlm:aff id="Aff2">Genomics and Molecular Medicine, Institute of Genomics and Integrative Biology (CSIR), Mall Road, Delhi, 110007 India</nlm:aff>
</affiliation>
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<author><name sortKey="Schenck, Annette" sort="Schenck, Annette" uniqKey="Schenck A" first="Annette" last="Schenck">Annette Schenck</name>
<affiliation><nlm:aff id="Aff3">Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff4">Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
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<author><name sortKey="Van Bokhoven, Hans" sort="Van Bokhoven, Hans" uniqKey="Van Bokhoven H" first="Hans" last="Van Bokhoven">Hans Van Bokhoven</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff3">Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff4">Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
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<author><name sortKey="Huys, Erik" sort="Huys, Erik" uniqKey="Huys E" first="Erik" last="Huys">Erik Huys</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
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<author><name sortKey="Schoenmakers, Eric F" sort="Schoenmakers, Eric F" uniqKey="Schoenmakers E" first="Eric F." last="Schoenmakers">Eric F. Schoenmakers</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Van Kessel, Ad Geurts" sort="Van Kessel, Ad Geurts" uniqKey="Van Kessel A" first="Ad Geurts" last="Van Kessel">Ad Geurts Van Kessel</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Van Nouhuys, C Erik" sort="Van Nouhuys, C Erik" uniqKey="Van Nouhuys C" first="C. Erik" last="Van Nouhuys">C. Erik Van Nouhuys</name>
<affiliation><nlm:aff id="Aff5">Canisius-Wilhelmina Ziekenhuis, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Cremers, Frans P M" sort="Cremers, Frans P M" uniqKey="Cremers F" first="Frans P. M." last="Cremers">Frans P. M. Cremers</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff3">Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>CDK19</italic>
is disrupted in a female patient with bilateral congenital retinal folds, microcephaly and mild mental retardation</title>
<author><name sortKey="Mukhopadhyay, Arijit" sort="Mukhopadhyay, Arijit" uniqKey="Mukhopadhyay A" first="Arijit" last="Mukhopadhyay">Arijit Mukhopadhyay</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff2">Genomics and Molecular Medicine, Institute of Genomics and Integrative Biology (CSIR), Mall Road, Delhi, 110007 India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kramer, Jamie M" sort="Kramer, Jamie M" uniqKey="Kramer J" first="Jamie M." last="Kramer">Jamie M. Kramer</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff3">Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff4">Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Merkx, Gerard" sort="Merkx, Gerard" uniqKey="Merkx G" first="Gerard" last="Merkx">Gerard Merkx</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lugtenberg, Dorien" sort="Lugtenberg, Dorien" uniqKey="Lugtenberg D" first="Dorien" last="Lugtenberg">Dorien Lugtenberg</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Smeets, Dominique F" sort="Smeets, Dominique F" uniqKey="Smeets D" first="Dominique F." last="Smeets">Dominique F. Smeets</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Oortveld, Merel A W" sort="Oortveld, Merel A W" uniqKey="Oortveld M" first="Merel A. W." last="Oortveld">Merel A. W. Oortveld</name>
<affiliation><nlm:aff id="Aff3">Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff4">Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Blokland, Ellen A W" sort="Blokland, Ellen A W" uniqKey="Blokland E" first="Ellen A. W." last="Blokland">Ellen A. W. Blokland</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Agrawal, Jyoti" sort="Agrawal, Jyoti" uniqKey="Agrawal J" first="Jyoti" last="Agrawal">Jyoti Agrawal</name>
<affiliation><nlm:aff id="Aff2">Genomics and Molecular Medicine, Institute of Genomics and Integrative Biology (CSIR), Mall Road, Delhi, 110007 India</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Schenck, Annette" sort="Schenck, Annette" uniqKey="Schenck A" first="Annette" last="Schenck">Annette Schenck</name>
<affiliation><nlm:aff id="Aff3">Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff4">Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Van Bokhoven, Hans" sort="Van Bokhoven, Hans" uniqKey="Van Bokhoven H" first="Hans" last="Van Bokhoven">Hans Van Bokhoven</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff3">Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff4">Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Huys, Erik" sort="Huys, Erik" uniqKey="Huys E" first="Erik" last="Huys">Erik Huys</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Schoenmakers, Eric F" sort="Schoenmakers, Eric F" uniqKey="Schoenmakers E" first="Eric F." last="Schoenmakers">Eric F. Schoenmakers</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Van Kessel, Ad Geurts" sort="Van Kessel, Ad Geurts" uniqKey="Van Kessel A" first="Ad Geurts" last="Van Kessel">Ad Geurts Van Kessel</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Van Nouhuys, C Erik" sort="Van Nouhuys, C Erik" uniqKey="Van Nouhuys C" first="C. Erik" last="Van Nouhuys">C. Erik Van Nouhuys</name>
<affiliation><nlm:aff id="Aff5">Canisius-Wilhelmina Ziekenhuis, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Cremers, Frans P M" sort="Cremers, Frans P M" uniqKey="Cremers F" first="Frans P. M." last="Cremers">Frans P. M. Cremers</name>
<affiliation><nlm:aff id="Aff1">Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</nlm:aff>
</affiliation>
<affiliation><nlm:aff id="Aff3">Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Human Genetics</title>
<idno type="ISSN">0340-6717</idno>
<idno type="eISSN">1432-1203</idno>
<imprint><date when="2010">2010</date>
</imprint>
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<front><div type="abstract" xml:lang="en"><p>Microcephaly, mental retardation and congenital retinal folds along with other systemic features have previously been reported as a separate clinical entity. The sporadic nature of the syndrome and lack of clear inheritance patterns pointed to a genetic heterogeneity. Here, we report a genetic analysis of a female patient with microcephaly, congenital bilateral falciform retinal folds, nystagmus, and mental retardation. Karyotyping revealed a de novo pericentric inversion in chromosome 6 with breakpoints in 6p12.1 and 6q21. Fluorescence in situ hybridization analysis narrowed down the region around the breakpoints, and the breakpoint at 6q21 was found to disrupt the <italic>CDK19</italic>
gene. <italic>CDK19</italic>
was found to be expressed in a diverse range of tissues including fetal eye and fetal brain. Quantitative PCR of the <italic>CDK19</italic>
transcript from Epstein–Barr virus-transformed lymphoblastoid cell lines of the patient revealed ~50% reduction in the transcript (<italic>p</italic>
= 0.02), suggesting haploinsufficiency of the gene. cdk8, the closest orthologue of human <italic>CDK19</italic>
in <italic>Drosophila</italic>
has been shown to play a major role in eye development. Conditional knock-down of <italic>Drosophila</italic>
cdk8 in multiple dendrite (md) neurons resulted in 35% reduced dendritic branching and altered morphology of the dendritic arbour, which appeared to be due in part to a loss of small higher order branches. In addition, Cdk8 mutant md neurons showed diminished dendritic fields revealing an important role of the CDK19 orthologue in the developing nervous system of <italic>Drosophila</italic>
. This is the first time the <italic>CDK19</italic>
gene, a component of the mediator co-activator complex, has been linked to a human disease.</p>
</div>
</front>
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</TEI>
<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Hum Genet</journal-id>
<journal-title-group><journal-title>Human Genetics</journal-title>
</journal-title-group>
<issn pub-type="ppub">0340-6717</issn>
<issn pub-type="epub">1432-1203</issn>
<publisher><publisher-name>Springer-Verlag</publisher-name>
<publisher-loc>Berlin/Heidelberg</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">20563892</article-id>
<article-id pub-id-type="pmc">2921488</article-id>
<article-id pub-id-type="publisher-id">848</article-id>
<article-id pub-id-type="doi">10.1007/s00439-010-0848-x</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Investigation</subject>
</subj-group>
</article-categories>
<title-group><article-title><italic>CDK19</italic>
is disrupted in a female patient with bilateral congenital retinal folds, microcephaly and mild mental retardation</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Mukhopadhyay</surname>
<given-names>Arijit</given-names>
</name>
<address><phone>+91-11-27666156</phone>
<fax>+91-11-27667471</fax>
<email>arijit@igib.res.in</email>
<email>arijit@igib.in</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kramer</surname>
<given-names>Jamie M.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
<xref ref-type="aff" rid="Aff3">3</xref>
<xref ref-type="aff" rid="Aff4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Merkx</surname>
<given-names>Gerard</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lugtenberg</surname>
<given-names>Dorien</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Smeets</surname>
<given-names>Dominique F.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Oortveld</surname>
<given-names>Merel A. W.</given-names>
</name>
<xref ref-type="aff" rid="Aff3">3</xref>
<xref ref-type="aff" rid="Aff4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Blokland</surname>
<given-names>Ellen A. W.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Agrawal</surname>
<given-names>Jyoti</given-names>
</name>
<xref ref-type="aff" rid="Aff2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Schenck</surname>
<given-names>Annette</given-names>
</name>
<xref ref-type="aff" rid="Aff3">3</xref>
<xref ref-type="aff" rid="Aff4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>van Bokhoven</surname>
<given-names>Hans</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
<xref ref-type="aff" rid="Aff3">3</xref>
<xref ref-type="aff" rid="Aff4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Huys</surname>
<given-names>Erik</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Schoenmakers</surname>
<given-names>Eric F.</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>van Kessel</surname>
<given-names>Ad Geurts</given-names>
</name>
<xref ref-type="aff" rid="Aff1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>van Nouhuys</surname>
<given-names>C. Erik</given-names>
</name>
<xref ref-type="aff" rid="Aff5">5</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Cremers</surname>
<given-names>Frans P. M.</given-names>
</name>
<address><phone>+31-24-3614017</phone>
<fax>+31-24-3668752</fax>
<email>f.cremers@antrg.umcn.nl</email>
</address>
<xref ref-type="aff" rid="Aff1">1</xref>
<xref ref-type="aff" rid="Aff3">3</xref>
</contrib>
<aff id="Aff1"><label>1</label>
Department of Human Genetics, Radboud University Nijmegen Medical Centre, PO Box 9101, 6500 HB Nijmegen, The Netherlands</aff>
<aff id="Aff2"><label>2</label>
Genomics and Molecular Medicine, Institute of Genomics and Integrative Biology (CSIR), Mall Road, Delhi, 110007 India</aff>
<aff id="Aff3"><label>3</label>
Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen, Nijmegen, The Netherlands</aff>
<aff id="Aff4"><label>4</label>
Donders Institute for Brain, Cognition and Behaviour, Radboud University Nijmegen, Nijmegen, The Netherlands</aff>
<aff id="Aff5"><label>5</label>
Canisius-Wilhelmina Ziekenhuis, Nijmegen, The Netherlands</aff>
</contrib-group>
<pub-date pub-type="epub"><day>22</day>
<month>6</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>22</day>
<month>6</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="ppub"><month>9</month>
<year>2010</year>
</pub-date>
<volume>128</volume>
<issue>3</issue>
<fpage>281</fpage>
<lpage>291</lpage>
<history><date date-type="received"><day>6</day>
<month>4</month>
<year>2010</year>
</date>
<date date-type="accepted"><day>3</day>
<month>6</month>
<year>2010</year>
</date>
</history>
<permissions><copyright-statement>© The Author(s) 2010</copyright-statement>
</permissions>
<abstract><p>Microcephaly, mental retardation and congenital retinal folds along with other systemic features have previously been reported as a separate clinical entity. The sporadic nature of the syndrome and lack of clear inheritance patterns pointed to a genetic heterogeneity. Here, we report a genetic analysis of a female patient with microcephaly, congenital bilateral falciform retinal folds, nystagmus, and mental retardation. Karyotyping revealed a de novo pericentric inversion in chromosome 6 with breakpoints in 6p12.1 and 6q21. Fluorescence in situ hybridization analysis narrowed down the region around the breakpoints, and the breakpoint at 6q21 was found to disrupt the <italic>CDK19</italic>
gene. <italic>CDK19</italic>
was found to be expressed in a diverse range of tissues including fetal eye and fetal brain. Quantitative PCR of the <italic>CDK19</italic>
transcript from Epstein–Barr virus-transformed lymphoblastoid cell lines of the patient revealed ~50% reduction in the transcript (<italic>p</italic>
= 0.02), suggesting haploinsufficiency of the gene. cdk8, the closest orthologue of human <italic>CDK19</italic>
in <italic>Drosophila</italic>
has been shown to play a major role in eye development. Conditional knock-down of <italic>Drosophila</italic>
cdk8 in multiple dendrite (md) neurons resulted in 35% reduced dendritic branching and altered morphology of the dendritic arbour, which appeared to be due in part to a loss of small higher order branches. In addition, Cdk8 mutant md neurons showed diminished dendritic fields revealing an important role of the CDK19 orthologue in the developing nervous system of <italic>Drosophila</italic>
. This is the first time the <italic>CDK19</italic>
gene, a component of the mediator co-activator complex, has been linked to a human disease.</p>
</abstract>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© Springer-Verlag 2010</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
<body><sec id="Sec1"><title>Introduction</title>
<p>Congenital retinal fold (ablatio falciformis congenita) refers to a congenital malformation that is characterized by the presence of a raised retinal fold extending radially from the posterior pole to the fundus periphery. It is reported that congenital retinal fold is not a single clinical entity, but can be a manifestation of a diverse group of conditions (van Nouhuys <xref ref-type="bibr" rid="CR37">1982a</xref>
). Familial exudative vitreoretinopathy (FEVR; MIM 133780) and microcephaly are frequently associated with congenital retinal fold (van Nouhuys <xref ref-type="bibr" rid="CR36">1981</xref>
, <xref ref-type="bibr" rid="CR37">1982a</xref>
). In OMIM two separate entries (MIM, 152950 and 156590) describe several reports in which the patients suffer from microcephaly, mild to moderate mental retardation, chorioretinopathy or congenital retinal fold, and lymphedema. The reports depict a heterogeneous clinical spectrum of these features with an uncertain inheritance pattern pointing towards a heterogeneous genetic etiology for this rare developmental syndrome.</p>
<p>In four eyes of three patients from two large families with FEVR, congenital retinal folds were found (van Nouhuys <xref ref-type="bibr" rid="CR37">1982a</xref>
). In the same report, it was mentioned that the formation of congenital retinal folds is the consequence of a developmental disorder of retinal vasculature during the last few months of intrauterine life and the folds may even develop after birth. The clinical picture and familial occurrence of many cases of congenital retinal folds previously described were suggestive of FEVR. Nishimura et al. (<xref ref-type="bibr" rid="CR28">1983</xref>
) likewise found falciform retinal folds as a sign of FEVR.</p>
<p>Several reports on patients with bilateral congenital retinal folds mention the presence of microcephaly with varied degree of mental retardation. The majority of the patients showed bilateral falciform folds extending from the optic disc to the inferior-temporal quadrant of the fundus periphery. The similarity of the retinal folds in this group of microcephalic patients suggested an independent clinical entity (van Nouhuys <xref ref-type="bibr" rid="CR38">1982b</xref>
).</p>
<p>Gartner (<xref ref-type="bibr" rid="CR8">1941</xref>
) and Masuda (<xref ref-type="bibr" rid="CR26">1962</xref>
) reported two independent exudative vitreoretinopathy patients, of which the parents were consanguineous, pointing to autosomal recessive transmission. On the other hand, Pfluger (<xref ref-type="bibr" rid="CR30">1885</xref>
) and Jarmas et al. (<xref ref-type="bibr" rid="CR14">1981</xref>
) reported patients of which one of the parents shared some, but not all of the clinical features, suggesting autosomal dominant transmission with variable expression. Recently, Nguyen et al. (<xref ref-type="bibr" rid="CR27">2005</xref>
) reported on a boy with congenital microcephaly, juvenile retinal dystrophy but without mental retardation. His eye examination revealed an optically empty vitreous and poorly developed fovea, pointing to the etiological relationship between this condition and FEVR.</p>
<p>In this study, we report the clinical and molecular findings in a female patient with bilateral congenital retinal folds and microcephaly with mild mental retardation. By cytogenetic and molecular genetic analyses, we have found that one copy of the <italic>CDK19</italic>
gene, located at 6q21, is disrupted in the patient as a result of a de novo pericentric inversion. In addition, we have used <italic>Drosophila</italic>
as a model system and identified a role of this gene in the developing nervous system.</p>
</sec>
<sec id="Sec2" sec-type="materials|methods"><title>Materials and methods</title>
<sec id="Sec3"><title>Clinical investigation</title>
<p>An informed consent was obtained from our index patient as well as from other individuals who has been recruited for the present study. Ophthalmological examination of the index patient has been performed on several occasions from 1979 to 2008. The following methods have been used: determination of visual acuity (Landolt C and Cardiff tests) and contrast sensitivity, applanation tonometry (Haag-Streit, Koeniz, Switzerland), gonioscopy, slit lamp examination of the anterior segment and vitreous (Rodenstock slit lamp, Munich, Germany), indirect ophthalmoscopy (Zeiss ophthalmoscope, Oberkochen, Germany), biomicroscopy (Rodenstock slit lamp), and digital fluorescein angiography of the fundi (Zeiss FF450 fundus camera, Win station software, Medivision-OIS, Sacramento, USA) and Goldmann perimetry (Haag-Streit perimeter, Munich, Germany). The patient has also been subjected to a full pediatric, neurological and otolaryngological examination.</p>
</sec>
<sec id="Sec4"><title>Cell culture</title>
<p>Human B-lymphocytes were immortalized by transformation with the Epstein–Barr virus (EBV) according to established procedures (Wall et al. <xref ref-type="bibr" rid="CR40">1995</xref>
). EBV-transformed lymphoblastoid cell lines (EBV–LCLs) of the patients and controls were grown to a density of 0.7 million cells per ml RPMI 1640 medium (Gibco, Breda, The Netherlands) containing 10% (v/v) fetal calf serum (Sigma, The Netherlands), 1% penicillin–streptomycin (Gibco, The Netherlands), and 1% GlutaMAX™ (Gibco, The Netherlands). Twenty-five million cells were harvested by centrifugation at 200×<italic>g</italic>
for 5 min at room temperature and re-suspended in 500 μl 8 mM Na<sub>2</sub>
HPO<sub>4</sub>
, 2 mM KH<sub>2</sub>
PO<sub>4</sub>
, 137 mM NaCl, 2.7 mM KCl pH 7.2 (PBS).</p>
</sec>
<sec id="Sec5"><title>Fluorescence in situ hybridization (FISH)</title>
<p>Non-radioactive in situ hybridization on human metaphase spreads was performed essentially as described earlier (de Bruijn et al. <xref ref-type="bibr" rid="CR5">2001</xref>
). The probes to be tested by FISH were selected from an array of 32,000 BAC probes covering the human genome (32 K BAC array). The analysis was performed on methanol/acetic acid fixed cells of cell lines from individuals under study. In short, BACs are biotin labeled by nick translation and then purified, and ethanol precipitated together with a 30-fold excess of Cot-1 DNA (Life Technologies). Immunocytochemical detection of the hybridizing probe was achieved using fluorescein isothiocyanate (FITC)-conjugated avidin followed by one application step of goat-anti-avidin conjugated with FITC.</p>
<p>The commercially available (Vysis) reference probe for centromere of chromosome 6, gives an orange fluorescence signal. A Zeiss Axiophot-2 microscope equipped with the appropriate fluorescence filters was used for analysis of the fluorescence signals. Images were captured by a Leica DC 350 FX camera using Leica CW 4000 software package.</p>
</sec>
<sec id="Sec6"><title>Semi-quantitative RT–PCR and real-time quantitative RT–PCR analysis</title>
<p>Total RNA was isolated from EBV-LCLs using the RNeasy midi-kit (Qiagen, Venlo, The Netherlands) according to the manufacturer’s protocol. The quality and quantity of the RNA was determined by spectrophotometric measurement and gel electrophoresis. cDNA was synthesized from 2.0 μg total RNA using random hexamers (Amersham, The Netherlands), and M-MLV reverse transcriptase (Invitrogen, The Netherlands) in a total volume of 85.0 μl. After the synthesis, the cDNA was purified using QIAquick PCR purification columns (Qiagen, Venlo, The Netherlands) and eluted in 30 μl milliQ water.</p>
<p>To determine the expression profile semi-quantitative RT–PCR was performed using primer pairs that amplify parts of <italic>CDK19</italic>
and <italic>ELOVL5</italic>
transcripts. The primer sequences for <italic>CDK19</italic>
was 5′-CGGAACCTATTTTTCACTGTCG-3′ (exon 7) and 5′-TGTGGGATATTCTGGCATCTT-3′ (exon 8) and that for <italic>ELOVL5</italic>
was 5′-CATTCAGACCTACAACAAGAAAGG-3′ (exons 6–7) and 5′-GGCTTCACATTGTTTTCCAGGG-3′ (exon 7). The PCR was performed for 30 cycles using RNA (cDNA) from 12 different human tissues. GAPDH was used as a reference gene.</p>
<p>For real-time quantitative RT–PCR (QPCR), 40× dilutions of the cDNA were made and 5.0 μl from the diluted stock was used for each PCR reaction. Primers were designed using Primer3 software (<ext-link ext-link-type="uri" xlink:href="http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi">http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi</ext-link>
). Amplicon lengths were typically kept between 80 and 130 bp. Quantitation was performed using SYBR-Green-based relative quantification strategies according to the manufacturer’s protocol (Bio-Rad, The Netherlands). PCR reactions were performed on an automatic iCycler (MyiQ single color Real-Time detection System, Bio-Rad, The Netherlands). Initially, the primer conditions were optimized and a standard curve was determined for six different dilution points (10×–320×) until for each of the primers PCR efficiencies close to 100% were obtained. For calculation purpose, all the PCR efficiencies were assumed to be exactly 100%. Two independent cDNA syntheses were made from each RNA sample and duplicate QPCR reactions were performed for each cDNA to ensure reproducibility. The following condition was used for all the QPCR experiments: initial denaturation for 3 min at 95.0°C, followed by 15 s at 95.0°C and 30 s at 60.0°C for 40 cycles (QPCR data collection). The products were then denatured at 95.0°C for 1 min and cooled to 65.0°C and kept for 1 min (melt curve data collection).</p>
<p>Ten different EBV–LCLs from 10 unrelated control samples from the Netherlands were used to determine the normal range of expression for <italic>CDK19</italic>
and <italic>ELOVL5</italic>
. The mean expression level was used for normalization. A recent report has systematically identified stable gene expressions in EBV–LCLs (de Brouwer et al. <xref ref-type="bibr" rid="CR4">2006</xref>
) for use as reference genes for normalization purpose. We have selected the most stable genes from this study, and after using Genorm (Vandesompele et al. <xref ref-type="bibr" rid="CR39">2002</xref>
) <italic>GUSB</italic>
and <italic>RNF111</italic>
were found to be two most stable genes in our experiments. The geometric mean of the expression of <italic>GusB</italic>
and <italic>RNF111</italic>
was used as the value for reference genes for normalization.</p>
<p>The differences in expression between patients and controls were calculated using the 2<sup>ΔΔCt</sup>
method (Livak and Schmittgen <xref ref-type="bibr" rid="CR23">2001</xref>
; Pfaffl <xref ref-type="bibr" rid="CR29">2001</xref>
). By definition, <italic>C</italic>
<sub>t</sub>
values equal the required number of amplification cycles to reach the threshold fluorescence level (automatically determined); Δ<italic>C</italic>
<sub>t</sub>
implies the difference in the <italic>C</italic>
<sub>t</sub>
values between the reference gene (mean) and the gene of interest (<italic>CDK19</italic>
or <italic>ELOVL5</italic>
) and ΔΔ<italic>C</italic>
<sub>t</sub>
represents the difference between two Δ<italic>C</italic>
<sub>t</sub>
values obtained from patient and the control (mean). For statistical analysis of the QPCR data, the <italic>p</italic>
values were calculated in the <italic>p</italic>
value calculator (<ext-link ext-link-type="uri" xlink:href="http://www.graphpad.com/quickcalcs/PValue1.cfm">www.graphpad.com/quickcalcs/PValue1.cfm</ext-link>
) available in the public domain.</p>
</sec>
<sec id="Sec7"><title>Drosophila studies</title>
<p>The UAS-Gal4 system (Brand and Perrimon <xref ref-type="bibr" rid="CR3">1993</xref>
) was used for induced expression of dsRNA and for labeling of neurons with GFP. The <italic>UAS-Cdk8</italic>
<sup>RNAi</sup>
lines were obtained from the National Institute of Genetics Fly Stock Center (stock numbers 10572R-1 and 10572R-3, Kyoto, Japan). These stocks contain an inducible 500 base pair dsRNA homologous to the <italic>Cdk8</italic>
coding region (exon 1 and part of exon 2). Details on the sequence and the absence of predictable off-targets are available at <ext-link ext-link-type="uri" xlink:href="http://www.shigen.nig.ac.jp/fly/nigfly/index.jsp">http://www.shigen.nig.ac.jp/fly/nigfly/index.jsp</ext-link>
. The ubiquitous promoter-Gal4 line <italic>y</italic>
<sup><italic>1</italic>
</sup>
<italic>w</italic>
<sup><italic>*</italic>
</sup>
<italic>;P{Act5C</italic>
-<italic>GAL4}25FO1/CyO</italic>
,<italic>y</italic>
<sup>+</sup>
(<italic>Act</italic>
-<italic>Gal4</italic>
) and <italic>y</italic>
<sup><italic>1</italic>
</sup>
<italic>w*;P[GawB]477,P[UAS</italic>
-<italic>mCD8::GFP.L]LL5 (477</italic>
-<italic>Gal4, UAS</italic>
-<italic>mCD8::GFP)</italic>
, which expresses a membrane targeted mCD8-GFP fusion protein in <italic>Drosophila</italic>
type 4 multiple dendrite (md) neurons (Grueber et al. <xref ref-type="bibr" rid="CR11">2003</xref>
), were obtained from the Bloomington Drosophila stock center at Indiana University (stock numbers 4414 and 8746, Bloomington, USA).</p>
<p>For analysis of <italic>Cdk8</italic>
knockdown efficiency <italic>Act</italic>
-<italic>Gal4</italic>
was balanced over a <italic>CyO</italic>
-<italic>GFP</italic>
chromosome. <italic>Act</italic>
-<italic>Gal4/CyO</italic>
-<italic>GFP</italic>
flies were then crossed to wild-type flies and to flies harboring the homozygously viable <italic>UAS</italic>
-<italic>Cdk8</italic>
<sup><italic>RNAi</italic>
</sup>
insertion. Third instar larval progeny that received the <italic>Act</italic>
-<italic>Gal4</italic>
transgene were identified based on the lack of GFP staining. <italic>Drosophila</italic>
total RNA was extracted in triplicate from third instar larvae of the genotypes <italic>UAS</italic>
-<italic>Cdk8</italic>
<sup><italic>RNAi</italic>
</sup>
<italic>/</italic>
+; <italic>Act</italic>
-<italic>Gal4/</italic>
+ and <italic>Act</italic>
-<italic>Gal4/</italic>
+ using the RNA Lipid Tissue mini kit (QIAGEN, Venlo, The Netherlands). RNA was transcribed into cDNA using the iScript cDNA Synthesis Kit (Bio-Rad, Veenendaal, The Netherlands) and cDNA was purified using Nucleospin Extract II PCR clean-up kit (Macherey–Nagel, Düren, Germany). SYBR-Green-based real-time quantitative PCR analysis was performed on a 7500 <italic>Fast</italic>
Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using Power SYBR-Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) according to the manufacturer’s instructions. Relative expression levels were calculated as described above using the <italic>Act5c</italic>
gene as a reference.</p>
<p>For analysis of dendritic development <italic>477</italic>
-<italic>Gal4, UAS</italic>
-<italic>mCD8</italic>
-<italic>GFP/CyO</italic>
flies were crossed to wild type and <italic>UAS</italic>
-<italic>Cdk8</italic>
<sup><italic>RNAi</italic>
</sup>
flies. Late third instar larvae expressing mCD8-GFP were dissected and immunostained using a rat anti-mCD8 antibody (1/200) (Invitrogen, Breda, The Netherlands) and an anti-rat Alexa Fluor 488 conjugated secondary antibody (1/1000) (Invitrogen, Breda, The Netherlands). A Leica DM-IRE2 confocal microscope (Leica Microsystems, Rijswijk, The Netherlands) was used to create images from a region of 375 × 375 μm between the denticle belts of the abdominal segments 3–5 of the wandering third instar larvae. Each larval hemi-segment contains four clusters of md sensory neurons (ventral, ventral’, lateral and dorsal), which are morphologically characterized and are positioned in a highly stereotypical pattern (Grueber et al. <xref ref-type="bibr" rid="CR10">2002</xref>
). In this study, we have analyzed the morphology of the solitary type 4 md dendritic arborization neuron in the ventral cluster called vdaB (ventral dendritic arborization neuron B). These neurons were chosen for analysis because of their highly stereotyped dendritic arbour that is amenable to quantitative morphological analysis. Microscopic images were captured using a 40× lens. The field of view was always confined by the denticle belts, on the upper (anterior) and lower (posterior) margin of the image, with the cell body of the vdaB neuron positioned approximately in the middle of the field of view from left to right (Fig. <xref rid="Fig5" ref-type="fig">5</xref>
d). Dendrite ends were counted manually from stacked images for the entire field of view. Ten images were quantified for each genotype and the mean number of dendrite ends was compared using a one sample <italic>t</italic>
test.</p>
</sec>
</sec>
<sec id="Sec8"><title>Results</title>
<sec id="Sec9"><title>A female patient with congenital retinal fold and microcephaly</title>
<p>The birth weight of the girl had been 2,350 g and the neonate was not treated with oxygen, a risk factor for retinopathy of prematurity. The pregnancy had been of normal duration, but had been complicated by an ovarian cyst, which had to be removed surgically soon after parturition. Her cranial circumference at birth was only 29 cm and remained far below the P10 curve on several subsequent occasions. Café-au-lait like pigmentations of the skin were found scattered over her body. Other changes that might be consistent with neurofibromatosis were not found. From infancy, a moderate psychomotor retardation and some hearing loss was found. An adenotomy and tonsillectomy were performed at age 4 after some recurrent upper airway infections. About 20 dB of hearing loss was diagnosed due to perceptive dysfunction, which did not show progression. MRI scans performed when she was an adult did not show congenital or acquired cerebral anomalies. The patient had been followed at regular intervals (once a year) for the last 29 years without any significant progression of her visual and psychomotor conditions. At the age of 1 year, she had been referred to an ophthalmologist for a convergent position of the left eye. She was found to have horizontal nystagmus when she was 2 years old. Fundus examination showed bilateral falciform retinal folds running from the optic nerve head to the temporal periphery. Both folds were attached by white fibrous tissue to the pars plana and temporal equator of the lens (Fig. <xref rid="Fig1" ref-type="fig">1</xref>
).<fig id="Fig1"><label>Fig. 1</label>
<caption><p>Fundus abnormalities in both eyes of the patient. <bold>a</bold>
<italic>Right eye</italic>
: posterior pole of fundus. A falciform retinal fold extends from the optic disc to the inferior-temporal quadrant of the fundus. Note the absence of the macula and abnormal course of retinal vessels. <bold>b</bold>
<italic>Left eye</italic>
posterior pole of the fundus. A falciform retinal fold extends from the disc in temporal direction. The retina superior to the fold shows some fibrous tissue</p>
</caption>
<graphic xlink:href="439_2010_848_Fig1_HTML" id="MO1"></graphic>
</fig>
</p>
<p>At the age of 14 years, visual acuity of the right eye and left eye was limited to finger counting. The left eye showed a convergent position. Both eyes showed a marked horizontal nystagmus. Cyclopic refraction showed a moderate hyperopic astigmatism of both eyes, but was difficult to determine because of poor fixation due to the prominent folds in the posterior poles. The anterior segments of both eyes showed a clear cornea with normal dimensions. No abnormalities of the iris and pupils were noted. Both lenses showed some capsular and sub-capsular opacity on the temporal side. The vitreous body of both eyes showed syneresis with some optically empty lacunae. Some preretinal membranes were noted temporally in both eyes. The right fundus showed marked deformation of the retinal vascular network in the posterior pole. A prominent retinal fold extended from the optic disc in inferior-temporal direction to the fundus periphery where it ended in a local mass of white fibrous tissue attached to the pars plana (Fig. <xref rid="Fig1" ref-type="fig">1</xref>
a). The macula was not visible and several retinal vessels extended from the disc in the retinal fold. In the posterior retina, a number of retinal vessels were visible outside the fold. Some of the vessels were seen to end in terminal ramifications. The peripheral retina presented an atrophic appearance and seemed avascular. No demarcation line could be observed between vascular and avascular retinal areas. Remnants of hyaloid vasculature could not be observed. The left fundus showed a similar falciform retinal fold running from the optic nerve head towards the temporal fundus periphery, where it disappeared in a white tissue mass attached to the lens equator and the pars plana (Fig. <xref rid="Fig1" ref-type="fig">1</xref>
b). The retina showed several pigmentations on either side of the fold and some fibrous epiretinal tissue especially superior to the fold.</p>
<p>At 38 years, gonioscopy was repeated because of some flattening of the anterior chamber of the left eye and moderate narrowing of the angle was noted. To prevent angle closure, an iridectomy was done with an Nd-YAG laser. No elevated intraocular pressure has been noticed before or after this procedure. Until her current age visual functions seemed to be stable. Eye examination including fundus biomicroscopy in the mother and father of the patient did not reveal significant abnormalities. A few maternal relatives showed unilateral visual impairment due to high myopia.</p>
<p>The patient was also found to have night blindness and lymphedema. She was subjected to several pediatric and neurological examinations at different ages. At the age of 41 years, type 2 diabetes was diagnosed and treated with diet and oral medication.</p>
</sec>
<sec id="Sec10"><title>A de novo pericentric inversion in chromosome 6</title>
<p>Karyotyping revealed a pericentric inversion in chromosome 6 of the patient involving 6p11.1 and 6q21 (Fig. <xref rid="Fig2" ref-type="fig">2</xref>
a). The patient otherwise had a normal female karyotype. The parents also showed normal karyotypes indicating that the patient did not inherit the chromosomal rearrangement.<fig id="Fig2"><label>Fig. 2</label>
<caption><p>Schematic representation of breakpoints on chromosome 6. <bold>a</bold>
Karyotyping showed a pericentric inversion in chromosome 6 which is schematically depicted in <bold>b</bold>
below; <bold>b</bold>
schemes of chromosome 6 for both the derivative (inverted) and the normal copy. The relative position and size of the FISH probes used are marked with corresponding colors on the <italic>right</italic>
; <bold>c</bold>
results of the FISH experiments performed with probes of the p-arm (<italic>top panel</italic>
) and the q-arm (<italic>middle panel</italic>
). In both cases, one of the copies (marked ‘inversion’) show a split signal positioning the breakpoint within the probe. The <italic>pink colored signal</italic>
is from the centromeric probe for chromosome 6. The <italic>lower panel</italic>
shows the fine mapping of 6q21 breakpoint using a PCR product as FISH probe; <bold>d</bold>
schematic summary of the entire breakpoint analysis along with the genes involved in or near the breakpoints and their genomic coordinates. The <italic>cartoon</italic>
shows that the inversion disrupts the <italic>CDK19</italic>
gene on 6q21, but not <italic>ELOVL5</italic>
on 6p12</p>
</caption>
<graphic xlink:href="439_2010_848_Fig2_HTML" id="MO2"></graphic>
</fig>
</p>
<p>To rule out other large copy number abnormalities in the patient’s genome and to narrow down the inversion breakpoints in the patient, comparative genomic hybridization was performed which did not show any clone to deviate significantly from the normal range of variation (data not shown). The karyotype for the derivative chromosome 6 indicated the breakpoint at 6p to be very close to the centromere. A location of the p-arm breakpoint in the centromere was excluded using FISH analysis of a centromeric probe for chromosome 6 (data not shown). Four BAC clones from 6q21, uniformly distributed across an 11-Mbp region, and six probes covering 10 Mbp at 6p11–p12 were selected from the 32K BAC array. Probes RP11-109D10 (C″ in Fig. <xref rid="Fig2" ref-type="fig">2</xref>
) and RP11-778B14 (C′ in Fig. <xref rid="Fig2" ref-type="fig">2</xref>
) were found to be broken at 6q21 and 6p12.1, respectively (Fig. <xref rid="Fig2" ref-type="fig">2</xref>
c). To confirm these findings, additional probes from both arms of chromosome 6 were selected which overlapped with the clones mentioned above. These clones, RP11-180C5 (6p12.1) and RP11-207N10 (6q21) also showed a split signal on the derivative chromosome mapping the breakpoints to 220 kb regions on either side of the centromere.</p>
</sec>
<sec id="Sec11"><title>Genes near the breakpoints</title>
<p>A more detailed inspection of the genomic regions covered by these BAC clones revealed that at 6p12.1 both RP11-778B14 and RP11-180C5 partially overlapped the <italic>ELOVL5</italic>
gene (Fig. <xref rid="Fig2" ref-type="fig">2</xref>
d). Similarly, at 6q21, RP11-109D10 completely and RP11-207N10 partially overlap <italic>CDK19</italic>
(Fig. <xref rid="Fig2" ref-type="fig">2</xref>
d). <italic>CDK19</italic>
and <italic>ELOVL5</italic>
were further investigated. Both <italic>CDK19</italic>
and <italic>ELOVL5</italic>
were found to have a wide range of tissue expression including fetal eye and fetal brain—the two tissues most likely to be involved in the developmental problems observed in the patient (Fig. <xref rid="Fig3" ref-type="fig">3</xref>
).<fig id="Fig3"><label>Fig. 3</label>
<caption><p>Expression profile of <italic>CDK19</italic>
and <italic>ELOVL5</italic>
. The normal expression profile of <italic>CDK19</italic>
and <italic>ELOVL5</italic>
was determined in 12 human tissues. Both genes are expressed in almost all tissues. Note the higher expression of <italic>CDK19</italic>
in fetal eye (<italic>lane 2</italic>
) and fetal brain (<italic>lane 7</italic>
) as compared to <italic>ELOVL5</italic>
. <italic>GAPDH</italic>
was used as a reference gene. The PCR was done for 30 cycles</p>
</caption>
<graphic xlink:href="439_2010_848_Fig3_HTML" id="MO3"></graphic>
</fig>
</p>
</sec>
<sec id="Sec12"><title><italic>CDK19</italic>
is disrupted, but <italic>ELOVL5</italic>
is intact in the patient</title>
<p>Parallel to the efforts to delineate the breakpoints, we quantified the amount of the transcript present in the patient for the genes mentioned above. As described in the “<xref rid="Sec2" ref-type="sec">Methods</xref>
” section, multiple reference genes have been used to normalize the data and RNA from 10 unrelated controls were used to assess the normal distribution of expression for <italic>CDK19</italic>
and <italic>ELOVL5</italic>
(Fig. <xref rid="Fig4" ref-type="fig">4</xref>
a). The transcript level for <italic>CDK19</italic>
in the patient was found to be 54% (<italic>p</italic>
= 0.022) when compared with the control mean, but that of <italic>ELOVL5</italic>
was not significantly reduced (Fig. <xref rid="Fig4" ref-type="fig">4</xref>
b). This indicates to the most likely situation that one copy of the <italic>CDK19</italic>
has become non-functional (haploinsufficiency) due to the pericentric inversion at 6q21. To further refine the breakpoint on 6q21, overlapping fragments of ~10 kb were generated by long-range PCR from intron 1 of <italic>CDK19</italic>
and these products were used as FISH probes. Owing to the repeat-rich nature of the template, most of these probes did not give reproducible results. However, one probe (C″ in Fig. <xref rid="Fig2" ref-type="fig">2</xref>
) shown complete translocation to the p-arm upon inversion (Fig. <xref rid="Fig2" ref-type="fig">2</xref>
c) placing the break-point distal to this probe. This data along with the data from two BAC clones as mentioned above almost certainly puts the 6q21 inversion breakpoint within intron 1 of <italic>CDK19</italic>
confirming our initial observations (Fig. <xref rid="Fig2" ref-type="fig">2</xref>
d). For 6p12.1, based on the transcript data, the breakpoint should be outside and proximal to <italic>ELOVL5</italic>
. To confirm that no partial duplication of the <italic>ELOVL5</italic>
gene is involved with this inversion, we have performed multiplex ligation-dependent probe amplification (MLPA) and genomic QPCR in the region of the probes overlapping with <italic>ELOVL5</italic>
and none of the probes showed any copy number changes (data not shown).<fig id="Fig4"><label>Fig. 4</label>
<caption><p>Relative quantitation of <italic>CDK19</italic>
and <italic>ELOVL5</italic>
transcripts. <bold>a</bold>
For relative quantitation of the transcript amounts of <italic>CDK19</italic>
and <italic>ELOVL5</italic>
in the patient, the expression levels were compared with that of the mean of 10 controls (1–10). The <italic>graph</italic>
also shows the mean value for the 10 controls and the four patients included in the study. Among these, the sample marked with ‘Inv. patient 1’ is the patient with the de novo pericentric inversion. <bold>b</bold>
The quantity of <italic>CDK19</italic>
and <italic>ELOVL5</italic>
transcripts of the inversion patient was compared with the control mean (<bold>a</bold>
). While the transcript amount for <italic>ELOVL5</italic>
was not significantly different and that for <italic>CDK19</italic>
was found to be only 54% compared to the controls, which was statistically significant (<italic>p</italic>
= 0.022). The results indicated that the patient is haploinsufficient for the <italic>CDK19</italic>
gene. All quantitation was done using the 2<sup>ΔΔCt</sup>
method after normalization with the geometric mean of the expression of <italic>GusB</italic>
and <italic>RNF111</italic>
for reference genes as described in the “<xref rid="Sec2" ref-type="sec">Methods</xref>
” section. <italic>p</italic>
value was calculated from the <italic>Z</italic>
score derived from the Δ<italic>C</italic>
<sub>t</sub>
values for expression</p>
</caption>
<graphic xlink:href="439_2010_848_Fig4_HTML" id="MO4"></graphic>
</fig>
</p>
</sec>
<sec id="Sec13"><title>Screening the <italic>CDK19</italic>
gene</title>
<p>The pericentric inversion event was de novo which most likely occurred in the germ line of one of the parents. To test whether the other <italic>CDK19</italic>
copy was involved, we sequenced the exons of this gene. However, no potentially pathologic sequence variant was identified in the open reading frame of <italic>CDK19</italic>
. We ascertained nine additional patients with similar phenotypic features. All of them showed a normal karyotype, and no mutations were found in the <italic>CDK19</italic>
open reading frame, and the transcript levels were normal in three of the nine patients in which RNA levels could be tested (AM, FPMC, unpublished data). In 100 sporadic patients with mental retardation in which the Fragile-X gene has been excluded, we also screened the <italic>CDK19</italic>
gene for deletions using three MLPA probes covering the gene. None of the patients shows an abnormal copy number suggesting that haploinsufficiency of <italic>CDK19</italic>
is not a common cause of mental retardation.</p>
</sec>
<sec id="Sec14"><title>The <italic>Drosophila</italic>
orthologue of <italic>CDK19</italic>
is a regulator of dendritic development</title>
<p>Because the patient exhibits developmental anomalies, we examined the physiological role of a <italic>CDK19</italic>
orthologue in the complex developing nervous system of <italic>Drosophila melanogaster</italic>
. Human CDK19 (NP_055891.1) is a paralogue of the CDK8 protein (NP_001251.1), and is also referred to as CDK8-like. The protein products of these two genes share 77% identity suggesting that they are functionally related. <italic>Drosophila</italic>
contains a single (two-to-one) orthologue to both proteins, Cdk8 (NP_536735.2) (Leclerc et al. <xref ref-type="bibr" rid="CR21">1996</xref>
), which shares 66% identity with human CDK19 and 71% identity with human CDK8. Construction of an unrooted phylogenetic tree using the neighbor-joining algorithm (<ext-link ext-link-type="uri" xlink:href="http://www.ebi.ac.uk/clustalw">http://www.ebi.ac.uk/clustalw</ext-link>
) (Saitou and Nei <xref ref-type="bibr" rid="CR32">1987</xref>
) shows that the <italic>Drosophila</italic>
Cdk8 protein is almost equally related to both CDK19 and CDK8 (Fig. <xref rid="Fig5" ref-type="fig">5</xref>
a). Furthermore, biochemical and genetic studies have confirmed a structural and functional conservation of <italic>Drosophila</italic>
Cdk8 when compared with yeast and mammalian models (Loncle et al. <xref ref-type="bibr" rid="CR24">2007</xref>
).<fig id="Fig5"><label>Fig. 5</label>
<caption><p>Functional analysis of the <italic>Drosophila CDK19</italic>
ortholog, <italic>Cdk8.</italic>
<bold>a</bold>
An unrooted phylogenetic tree comparing CDK8 protein family members in human (CDK8 and CDK19) and <italic>Drosophila</italic>
(Cdk8) by the neighbor-joining method. <bold>b</bold>
Relative expression of <italic>Cdk8</italic>
mRNA was measured by quantitative PCR. <italic>Cdk8</italic>
mRNA is reduced by 68% in <italic>Cdk8(RNAi)</italic>
flies (genotype <italic>ActGal4/</italic>
+; <italic>UAS</italic>
-<italic>Cdk8(RNAi)/</italic>
+) when compared with controls (genotype <italic>ActGal4/</italic>
+; +<italic>/</italic>
+). <bold>c</bold>
Dendrite ends were counted manually from confocal images of type 4 md neurons (<bold>d</bold>
). Dendritic branching was reduced by 35% in <italic>Cdk8(RNA</italic>
i) flies (genotype <italic>477</italic>
-<italic>Gal4,UAS</italic>
-<italic>mCD8::GFP/</italic>
+; <italic>UAS</italic>
-<italic>Cdk8(RNAi)/</italic>
+) when compared with controls (genotype <italic>477</italic>
-<italic>Gal4,UAS</italic>
-<italic>mCD8::GFP/</italic>
+; +<italic>/</italic>
+). <bold>d</bold>
Representative images of ventral type 4 md neurons located in the larval skin. Cdk8 knockdown in these cells (<italic>right panel</italic>
) causes a decrease in dendritic branching (quantified in <bold>c</bold>
) and restriction of the overall dendritic arbor when compared with controls (<italic>left panel</italic>
). The <italic>stippled line</italic>
shows an area of restricted growth where a neighboring neuron (the v’ada type 4 md neuron from the ventral’ cluster) has grown. This altered neuronal morphology is not observed in controls. <italic>Red dots</italic>
in the <italic>bottom panel</italic>
show dendritic protrusions of <10 μm in length. These are more common in control neurons when compared with Cdk8-knockdown neurons (44 as compared to 31 in this one representative panel). Posterior is up. <italic>Scale bar</italic>
50 μm in the <italic>upper panel</italic>
and 10 μm in the <italic>lower panel</italic>
</p>
</caption>
<graphic xlink:href="439_2010_848_Fig5_HTML" id="MO5"></graphic>
</fig>
</p>
<p>To examine the function of <italic>Cdk8</italic>
in fly development, we first performed a ubiquitous knockdown of this gene by driving expression of an <italic>UAS</italic>
-<italic>Cdk8</italic>
<sup><italic>RNAi</italic>
</sup>
transgene using the <italic>Act</italic>
-<italic>Gal4</italic>
driver. Ubiquitous knockdown of <italic>cdk8</italic>
resulted in lethality at pupal and pharate adult stages. Analysis of the knock-down efficiency in third instar larvae showed that <italic>Cdk8</italic>
mRNA was significantly reduced by 68% (<italic>p</italic>
< 0.05) when compared with controls (Fig. <xref rid="Fig5" ref-type="fig">5</xref>
b) demonstrating that partial activity of this gene is not sufficient to achieve normal development. This is consistent with the phenotype observed in <italic>Cdk8</italic>
null mutant flies, which also show developmental lethality (Loncle et al. <xref ref-type="bibr" rid="CR24">2007</xref>
) and the haploinsufficiency of this gene seen in our patient.</p>
<p>Because the neuronal pathology in our patient is uncertain, but dendritic abnormalities are commonly observed in MR (Kaufmann and Moser <xref ref-type="bibr" rid="CR16">2000</xref>
), we examined whether Cdk8 affects dendritic development in the <italic>Drosophila</italic>
type 4 md sensory neurons located in the peripheral nervous system of the <italic>Drosophila</italic>
larvae. These neurons are amenable to quantitative analysis and have previously been shown to be relevant in other fly models for mental retardation (Lee et al. <xref ref-type="bibr" rid="CR22">2003</xref>
; Lu et al. <xref ref-type="bibr" rid="CR25">2009</xref>
). We found that knockdown of <italic>Cdk8</italic>
in <italic>Drosophila</italic>
type 4 md neurons by expression of <italic>UAS</italic>
-<italic>Cdk8</italic>
<sup><italic>RNAi</italic>
</sup>
with the <italic>477</italic>
-<italic>Gal4</italic>
driver resulted in reduced dendritic branching and altered morphology of the dendritic arbour (Fig. <xref rid="Fig5" ref-type="fig">5</xref>
c, d). Quantification of dendritic branching revealed a 35% reduction (<italic>p</italic>
< 0.001) in dendrite ends upon Cdk8 knockdown (Fig. <xref rid="Fig5" ref-type="fig">5</xref>
c), which appeared to be due in part to a loss of small dendritic protrusions of a length <10 μm (Fig. <xref rid="Fig5" ref-type="fig">5</xref>
d). This trend was seen consistently and is highlighted in the lower panels of Fig. <xref rid="Fig5" ref-type="fig">5</xref>
d where such protrusions (marked by red dots) are 35% less in mutant than in control neurons. A similar defect was also observed with a second <italic>UAS</italic>
-<italic>Cdk8</italic>
<sup><italic>RNAi</italic>
</sup>
line indicating that this phenotype does not result from non-specific effects due to insertion of the transgene. In addition, 90% of dendritic arbours examined for <italic>Cdk8</italic>
knockdown showed restricted extension and did not occupy the full field of view examined by confocal microscopy (Fig. <xref rid="Fig5" ref-type="fig">5</xref>
d). In many cases, the unoccupied area was filled by a more faintly stained type 4 md neuron from the ventral cluster (shown in Fig. <xref rid="Fig5" ref-type="fig">5</xref>
d) and in some cases no other dendrites were stained in the ‘unoccupied’ area (data not shown). In contrast, wild-type neurons always occupy nearly the entire field of view demonstrating a clear morphological defect in resulting from knockdown of the CDK19 orthologue in these neurons.</p>
</sec>
</sec>
<sec id="Sec15"><title>Discussion</title>
<p>In this article, we described that the microcephaly, congenital retinal fold and mild mental retardation is likely caused by <italic>CDK19</italic>
haploinsufficiency in a female Caucasian patient born to non-consanguineous parents. <italic>CDK19</italic>
expression was found in a wide variety of human tissues, with high expression in fetal brain and fetal retina (Fig. <xref rid="Fig3" ref-type="fig">3</xref>
), the two tissues involved in the phenotypes observed in the patient. Furthermore, our functional analysis in <italic>Drosophila</italic>
shows that the closest CDK19 orthologue, Cdk8, is a regulator of dendritic development, an important factor in the etiology of mental retardation (Kaufmann and Moser <xref ref-type="bibr" rid="CR16">2000</xref>
). In addition, recent reports showed that loss of CDK8 in <italic>Drosophila</italic>
disrupts the neural wiring of the eye and development of sensory bristles in the peripheral nervous system (Loncle et al. <xref ref-type="bibr" rid="CR24">2007</xref>
; Berger et al. <xref ref-type="bibr" rid="CR2">2008</xref>
), implying a role for Cdk8 both in dendritic development and in development of peripheral sensory organs including the eye.</p>
<p>Because Drosophila contains only one orthologue to the mammalian paralogues CDK19 and CDK8, analysis in this system is not confounded by potential redundancies between CDK19 and CDK8 that may occur in mammalian model systems and in humans. This is likely to contribute to the more dramatic phenotypes (e.g. lethality) in the model as compared to our patient.</p>
<p><italic>CDK19</italic>
encodes a protein that is one of the components of the mediator co-activator complex. The mediator complex is a multi-protein complex required for transcriptional activation by DNA-binding transcription factors for genes transcribed by RNA polymerase II (pol II). The protein is 77% identical to cyclin-dependent kinase 8 (CDK8), which is another component of the mediator complex (Elmlund et al. <xref ref-type="bibr" rid="CR7">2006</xref>
).</p>
<p>CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8-submodule), which inhibits the transcriptional coactivator function of the mediator complex by prevention of interactions with pol II and can operate independently of the mediator (Elmlund et al. <xref ref-type="bibr" rid="CR7">2006</xref>
; Knuesel et al. <xref ref-type="bibr" rid="CR18">2009a</xref>
, <xref ref-type="bibr" rid="CR19">b</xref>
). Based on the homology with CDK8, it is reasonable to predict that CDK19 also has related functions. Interestingly, the CDK8-submodule of the mediator complex is linked to mental retardation through <italic>MED12</italic>
, a causative gene for FG syndrome, a neurological disorder characterized by mental retardation, macrocephaly, hypotonia and constipation (Risheg et al. <xref ref-type="bibr" rid="CR31">2007</xref>
). MED12 is known to regulate neuronal gene expression through cooperation with the neuronal gene silencing factor REST and euchromatin histone methyltransferase 2 (EHMT2/G9a) (Ding et al. <xref ref-type="bibr" rid="CR6">2008</xref>
). EHMT2/G9a activity is dependent on interactions with its close homolog EHMT1 (Tachibana et al. <xref ref-type="bibr" rid="CR33">2005</xref>
), which is involved in Kleefstra syndrome, a neurological disorder characterized by mental retardation and other morphological and behavioral phenotypes (Kleefstra et al. <xref ref-type="bibr" rid="CR17">2006</xref>
). EHMT1 and EHMT2 have also been linked to REST transcriptional activity through interactions with the histone demethylase JARID1c (Tahiliani et al. <xref ref-type="bibr" rid="CR34">2007</xref>
), yet another mental retardation gene with a known role in dendritic development (Iwase et al. <xref ref-type="bibr" rid="CR13">2007</xref>
; Jensen et al. <xref ref-type="bibr" rid="CR15">2005</xref>
). Taken together, these data strongly suggest that the protein complexes containing CDK19 form a network of transcriptional repressors that effect neuronal development and function and contribute to the etiology of mental retardation. However, a possibility cannot be excluded that in human and other mammals, CDK8 and CDK19 work in synergy, and lack of one molecule can be compensated by the other.</p>
<p>Another important cyclin-dependent kinase, CDKL5, has been associated with atypical forms of Rett syndrome (RS), an X-linked neurological disorder, characterized by microcephaly, seizures and mental retardation (Tao et al. <xref ref-type="bibr" rid="CR35">2004</xref>
; Weaving et al. <xref ref-type="bibr" rid="CR41">2005</xref>
). CDK19 has 32% identity and 49% similarity with CDKL5 at the amino acid level. The different phenotype described in this study and the eye phenotypes might be explained by the differences in spatial and temporal expression of the two genes and having different targets for phosphorylation. A large deletion in patients with X-linked retinoschisis (XLR, MIM 312700) was found to have disrupted at least the last coding exon of <italic>CDKL5</italic>
, in addition to disruption of the <italic>RS1</italic>
gene (Huopaniemi et al. <xref ref-type="bibr" rid="CR12">2000</xref>
). One of the patients had congenital nystagmus, which we also observed in our patient. Interestingly, XLRS patients also show splits and breaks in retina and occasionally retinal detachment, which are features of other vitreoretinopathies as well as overlaps with the features in the patient described in this study.</p>
<p>Recently, a growing number of studies have shown the involvement of ciliary proteins in diseases involving mental retardation (Badano et al. <xref ref-type="bibr" rid="CR1">2006</xref>
). Some examples include Bardet–Biedl syndrome (BBS, MIM 209900), Meckel–Gruber syndrome (MKS1, MIM 249000 and MKS2, MIM 603194), and Joubert syndrome (MIM 610188). Bardet–Biedl and Joubert syndrome also manifest microcephaly and mental retardation in addition to retinal degeneration in some cases. We hypothesized that CDK19 might be present in the cilia and found it in the ciliary proteome database (Gherman et al. <xref ref-type="bibr" rid="CR9">2006</xref>
). It was reported that the BBS patients have olfaction defects (anosmia) which has been suggested to be a general manifestation of ciliary protein defects (Kulaga et al. <xref ref-type="bibr" rid="CR20">2004</xref>
). To test our hypothesis, we have performed the scratch-n-sniff test for our patient which revealed that she is highly anosmic (score 16 at the age of 41 years). However, this data can be confounded due to underdeveloped mental abilities of the patient.</p>
<p>In this article, we have described a female patient with microcephaly, congenital retinal fold and mild mental retardation, who has a pericentric inversion in one copy of chromosome 6, causing haploinsufficiency of <italic>CDK19</italic>
gene. The expression profile of <italic>CDK19</italic>
, its homology with other serine/threonine kinases, and its role in the in <italic>Drosophila</italic>
nervous system development, together strongly suggests <italic>CDK19</italic>
as an important determining factor for this syndrome.</p>
</sec>
</body>
<back><ack><p>The authors thank Drs. Arjan P.M. de Brouwer, Anneke I. den Hollander and Karin W. Littink for their help during various phases of the work. Dr. K. Devriendt from Leuven (Belgium) is acknowledged for providing us with DNA samples from six patients with similar phenotypes. Thanks also to Astrid Oudakker, Saskia D. van der Velde-Visser, Christel Beumer and Tannavi Kiran for expert technical assistance. This study was supported by European Union Research Training Network Grant RETNET MRTN-CT-2003-504003, a Vidi grant from The Netherlands Organization for Scientific Research (NWO) to AS and Council of Scientific and Industrial Research (CSIR), India funded project, “An Integrative Biology Approach in Deciphering Genotype–Phenotype Correlation for Human Complex Disorders” (SIP-0006).</p>
<p><bold>Open Access</bold>
This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.</p>
</ack>
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<fn-group><fn><p>A. Mukhopadhyay and J. M. Kramer contributed equally to this work.</p>
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