Ectodomain Shedding of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE-1) Is Induced by Vascular Endothelial Growth Factor A (VEGF-A)*
Identifieur interne : 000658 ( Pmc/Corpus ); précédent : 000657; suivant : 000659Ectodomain Shedding of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE-1) Is Induced by Vascular Endothelial Growth Factor A (VEGF-A)*
Auteurs : Hisayo Nishida-Fukuda ; Ryoichi Araki ; Masachika Shudou ; Hidenori Okazaki ; Yasuko Tomono ; Hironao Nakayama ; Shinji Fukuda ; Tomohisa Sakaue ; Yuji Shirakata ; Koji Sayama ; Koji Hashimoto ; Michael Detmar ; Shigeki Higashiyama ; Satoshi HirakawaSource :
- The Journal of Biological Chemistry [ 0021-9258 ] ; 2016.
Abstract
Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), a type I transmembrane glycoprotein, is known as one of the most specific lymphatic vessel markers in the skin. In this study, we found that the ectodomain of LYVE-1 undergoes proteolytic cleavage, and this process produces soluble LYVE-1. We further identified the cleavage site for ectodomain shedding and generated an uncleavable mutant of LYVE-1. In lymphatic endothelial cells, ectodomain shedding of LYVE-1 was induced by vascular endothelial growth factor (VEGF)-A, an important factor for angiogenesis and lymphangiogenesis under pathological conditions. VEGF-A-induced LYVE-1 ectodomain shedding was mediated via the extracellular signal-regulated kinase (ERK) and a disintegrin and metalloproteinase (ADAM) 17. Wild-type LYVE-1, but not uncleavable LYVE-1, promoted migration of lymphatic endothelial cells in response to VEGF-A. Immunostaining analyses in human psoriasis skin lesions and VEGF-A transgenic mouse skin suggested that the ectodomain shedding of LYVE-1 occurred in lymphatic vessels undergoing chronic inflammation. These results indicate that the ectodomain shedding of LYVE-1 might be involved in promoting pathological lymphangiogenesis.
Url:
DOI: 10.1074/jbc.M115.683201
PubMed: 26966180
PubMed Central: 4865900
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PMC:4865900Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Ectodomain Shedding of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE-1) Is Induced by Vascular Endothelial Growth Factor A (VEGF-A)<xref ref-type="fn" rid="FN1">*</xref></title><author><name sortKey="Nishida Fukuda, Hisayo" sort="Nishida Fukuda, Hisayo" uniqKey="Nishida Fukuda H" first="Hisayo" last="Nishida-Fukuda">Hisayo Nishida-Fukuda</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation></author><author><name sortKey="Araki, Ryoichi" sort="Araki, Ryoichi" uniqKey="Araki R" first="Ryoichi" last="Araki">Ryoichi Araki</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Shudou, Masachika" sort="Shudou, Masachika" uniqKey="Shudou M" first="Masachika" last="Shudou">Masachika Shudou</name><affiliation><nlm:aff id="aff3">Bioscience, Advanced Research Support Center (ADRES), Ehime University Graduate School of Medicine, Toon 791-0295, Japan,</nlm:aff></affiliation></author><author><name sortKey="Okazaki, Hidenori" sort="Okazaki, Hidenori" uniqKey="Okazaki H" first="Hidenori" last="Okazaki">Hidenori Okazaki</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tomono, Yasuko" sort="Tomono, Yasuko" uniqKey="Tomono Y" first="Yasuko" last="Tomono">Yasuko Tomono</name><affiliation><nlm:aff id="aff4">Division of Molecular and Cell Biology, Shigei Medical Research Institute, Okayama 701-0202, Japan,</nlm:aff></affiliation></author><author><name sortKey="Nakayama, Hironao" sort="Nakayama, Hironao" uniqKey="Nakayama H" first="Hironao" last="Nakayama">Hironao Nakayama</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan 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id="aff6">Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</nlm:aff></affiliation></author><author><name sortKey="Shirakata, Yuji" sort="Shirakata, Yuji" uniqKey="Shirakata Y" first="Yuji" last="Shirakata">Yuji Shirakata</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Sayama, Koji" sort="Sayama, Koji" uniqKey="Sayama K" first="Koji" last="Sayama">Koji Sayama</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hashimoto, Koji" sort="Hashimoto, Koji" uniqKey="Hashimoto K" first="Koji" last="Hashimoto">Koji Hashimoto</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name><affiliation><nlm:aff wicri:cut=", and" id="aff7">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich CH-8093, Switzerland</nlm:aff></affiliation></author><author><name sortKey="Higashiyama, Shigeki" sort="Higashiyama, Shigeki" uniqKey="Higashiyama S" first="Shigeki" last="Higashiyama">Shigeki Higashiyama</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan,</nlm:aff></affiliation><affiliation><nlm:aff id="aff6">Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</nlm:aff></affiliation></author><author><name sortKey="Hirakawa, Satoshi" sort="Hirakawa, Satoshi" uniqKey="Hirakawa S" first="Satoshi" last="Hirakawa">Satoshi Hirakawa</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan,</nlm:aff></affiliation><affiliation><nlm:aff id="aff8">Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">26966180</idno><idno type="pmc">4865900</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4865900</idno><idno type="RBID">PMC:4865900</idno><idno type="doi">10.1074/jbc.M115.683201</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">000658</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000658</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Ectodomain Shedding of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE-1) Is Induced by Vascular Endothelial Growth Factor A (VEGF-A)<xref ref-type="fn" rid="FN1">*</xref></title><author><name sortKey="Nishida Fukuda, Hisayo" sort="Nishida Fukuda, Hisayo" uniqKey="Nishida Fukuda H" first="Hisayo" last="Nishida-Fukuda">Hisayo Nishida-Fukuda</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation></author><author><name sortKey="Araki, Ryoichi" sort="Araki, Ryoichi" uniqKey="Araki R" first="Ryoichi" last="Araki">Ryoichi Araki</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Shudou, Masachika" sort="Shudou, Masachika" uniqKey="Shudou M" first="Masachika" last="Shudou">Masachika Shudou</name><affiliation><nlm:aff id="aff3">Bioscience, Advanced Research Support Center (ADRES), Ehime University Graduate School of Medicine, Toon 791-0295, Japan,</nlm:aff></affiliation></author><author><name sortKey="Okazaki, Hidenori" sort="Okazaki, Hidenori" uniqKey="Okazaki H" first="Hidenori" last="Okazaki">Hidenori Okazaki</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tomono, Yasuko" sort="Tomono, Yasuko" uniqKey="Tomono Y" first="Yasuko" last="Tomono">Yasuko Tomono</name><affiliation><nlm:aff id="aff4">Division of Molecular and Cell Biology, Shigei Medical Research Institute, Okayama 701-0202, Japan,</nlm:aff></affiliation></author><author><name sortKey="Nakayama, Hironao" sort="Nakayama, Hironao" uniqKey="Nakayama H" first="Hironao" last="Nakayama">Hironao Nakayama</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan,</nlm:aff></affiliation><affiliation><nlm:aff id="aff6">Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</nlm:aff></affiliation></author><author><name sortKey="Fukuda, Shinji" sort="Fukuda, Shinji" uniqKey="Fukuda S" first="Shinji" last="Fukuda">Shinji Fukuda</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation><affiliation><nlm:aff id="aff6">Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</nlm:aff></affiliation></author><author><name sortKey="Sakaue, Tomohisa" sort="Sakaue, Tomohisa" uniqKey="Sakaue T" first="Tomohisa" last="Sakaue">Tomohisa Sakaue</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation><affiliation><nlm:aff id="aff6">Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</nlm:aff></affiliation></author><author><name sortKey="Shirakata, Yuji" sort="Shirakata, Yuji" uniqKey="Shirakata Y" first="Yuji" last="Shirakata">Yuji Shirakata</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Sayama, Koji" sort="Sayama, Koji" uniqKey="Sayama K" first="Koji" last="Sayama">Koji Sayama</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hashimoto, Koji" sort="Hashimoto, Koji" uniqKey="Hashimoto K" first="Koji" last="Hashimoto">Koji Hashimoto</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name><affiliation><nlm:aff wicri:cut=", and" id="aff7">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich CH-8093, Switzerland</nlm:aff></affiliation></author><author><name sortKey="Higashiyama, Shigeki" sort="Higashiyama, Shigeki" uniqKey="Higashiyama S" first="Shigeki" last="Higashiyama">Shigeki Higashiyama</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan,</nlm:aff></affiliation><affiliation><nlm:aff id="aff6">Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</nlm:aff></affiliation></author><author><name sortKey="Hirakawa, Satoshi" sort="Hirakawa, Satoshi" uniqKey="Hirakawa S" first="Satoshi" last="Hirakawa">Satoshi Hirakawa</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff5">Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan,</nlm:aff></affiliation><affiliation><nlm:aff id="aff8">Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan</nlm:aff></affiliation></author></analytic><series><title level="j">The Journal of Biological Chemistry</title><idno type="ISSN">0021-9258</idno><idno type="eISSN">1083-351X</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), a type I transmembrane glycoprotein, is known as one of the most specific lymphatic vessel markers in the skin. In this study, we found that the ectodomain of LYVE-1 undergoes proteolytic cleavage, and this process produces soluble LYVE-1. We further identified the cleavage site for ectodomain shedding and generated an uncleavable mutant of LYVE-1. In lymphatic endothelial cells, ectodomain shedding of LYVE-1 was induced by vascular endothelial growth factor (VEGF)-A, an important factor for angiogenesis and lymphangiogenesis under pathological conditions. VEGF-A-induced LYVE-1 ectodomain shedding was mediated via the extracellular signal-regulated kinase (ERK) and a disintegrin and metalloproteinase (ADAM) 17. Wild-type LYVE-1, but not uncleavable LYVE-1, promoted migration of lymphatic endothelial cells in response to VEGF-A. Immunostaining analyses in human psoriasis skin lesions and VEGF-A transgenic mouse skin suggested that the ectodomain shedding of LYVE-1 occurred in lymphatic vessels undergoing chronic inflammation. These results indicate that the ectodomain shedding of LYVE-1 might be involved in promoting pathological lymphangiogenesis.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Biol Chem</journal-id><journal-id journal-id-type="iso-abbrev">J. Biol. Chem</journal-id><journal-id journal-id-type="hwp">jbc</journal-id><journal-id journal-id-type="pmc">jbc</journal-id><journal-id journal-id-type="publisher-id">JBC</journal-id><journal-title-group><journal-title>The Journal of Biological Chemistry</journal-title></journal-title-group><issn pub-type="ppub">0021-9258</issn><issn pub-type="epub">1083-351X</issn><publisher><publisher-name>American Society for Biochemistry and Molecular Biology</publisher-name><publisher-loc>11200 Rockville Pike, Suite 302, Rockville, MD 20852-3110, U.S.A.</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26966180</article-id><article-id pub-id-type="pmc">4865900</article-id><article-id pub-id-type="publisher-id">M115.683201</article-id><article-id pub-id-type="doi">10.1074/jbc.M115.683201</article-id><article-categories><subj-group subj-group-type="heading"><subject>Cell Biology</subject></subj-group></article-categories><title-group><article-title>Ectodomain Shedding of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE-1) Is Induced by Vascular Endothelial Growth Factor A (VEGF-A)<xref ref-type="fn" rid="FN1">*</xref></article-title><alt-title alt-title-type="short">VEGF-induced LYVE-1 Shedding in Lymphatic Endothelial Cells</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Nishida-Fukuda</surname><given-names>Hisayo</given-names></name><xref ref-type="aff" rid="aff1"><sup>‡</sup></xref><xref ref-type="aff" rid="aff2"><sup>§</sup></xref></contrib><contrib contrib-type="author"><name><surname>Araki</surname><given-names>Ryoichi</given-names></name><xref ref-type="aff" rid="aff1"><sup>‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Shudou</surname><given-names>Masachika</given-names></name><xref ref-type="aff" rid="aff3"><sup>¶</sup></xref></contrib><contrib contrib-type="author"><name><surname>Okazaki</surname><given-names>Hidenori</given-names></name><xref ref-type="aff" rid="aff1"><sup>‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Tomono</surname><given-names>Yasuko</given-names></name><xref ref-type="aff" rid="aff4"><sup>‖</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nakayama</surname><given-names>Hironao</given-names></name><xref ref-type="aff" rid="aff2"><sup>§</sup></xref><xref ref-type="aff" rid="aff5">**</xref><xref ref-type="aff" rid="aff6"><sup>‡‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Fukuda</surname><given-names>Shinji</given-names></name><xref ref-type="aff" rid="aff2"><sup>§</sup></xref><xref ref-type="aff" rid="aff6"><sup>‡‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sakaue</surname><given-names>Tomohisa</given-names></name><xref ref-type="aff" rid="aff2"><sup>§</sup></xref><xref ref-type="aff" rid="aff6"><sup>‡‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Shirakata</surname><given-names>Yuji</given-names></name><xref ref-type="aff" rid="aff1"><sup>‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sayama</surname><given-names>Koji</given-names></name><xref ref-type="aff" rid="aff1"><sup>‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Hashimoto</surname><given-names>Koji</given-names></name><xref ref-type="aff" rid="aff1"><sup>‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Detmar</surname><given-names>Michael</given-names></name><xref ref-type="aff" rid="aff7"><sup>§§</sup></xref></contrib><contrib contrib-type="author"><name><surname>Higashiyama</surname><given-names>Shigeki</given-names></name><xref ref-type="aff" rid="aff2"><sup>§</sup></xref><xref ref-type="aff" rid="aff5">**</xref><xref ref-type="aff" rid="aff6"><sup>‡‡</sup></xref></contrib><contrib contrib-type="author"><name><surname>Hirakawa</surname><given-names>Satoshi</given-names></name><xref ref-type="aff" rid="aff1"><sup>‡</sup></xref><xref ref-type="aff" rid="aff5">**</xref><xref ref-type="aff" rid="aff8"><sup>¶¶</sup></xref><xref ref-type="corresp" rid="cor1"><sup>1</sup></xref></contrib><aff id="aff1">From the Departments of<label>‡</label>Dermatology,</aff><aff id="aff2"><label>§</label>Biochemistry and Molecular Genetics, and</aff><aff id="aff3"><label>¶</label>Bioscience, Advanced Research Support Center (ADRES), Ehime University Graduate School of Medicine, Toon 791-0295, Japan,</aff><aff id="aff4"><label>‖</label>Division of Molecular and Cell Biology, Shigei Medical Research Institute, Okayama 701-0202, Japan,</aff><aff id="aff5"><label>**</label>Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan,</aff><aff id="aff6"><label>‡‡</label>Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</aff><aff id="aff7"><label>§§</label>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich CH-8093, Switzerland, and</aff><aff id="aff8"><label>¶¶</label>Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan</aff></contrib-group><author-notes><corresp id="cor1"><label>1</label> To whom correspondence should be addressed: <addr-line>Dept. of Dermatology, Hamamatsu University School of Medicine, Handayama 1-20-1, Higashi-ku, Hamamatsu 431-3192, Japan.</addr-line> Tel.: <phone>81-53-435-2065</phone>; Fax: <fax>81-53-435-2368</fax>; E-mail: <email>hirakawa@hama-med.ac.jp</email>.</corresp></author-notes><pub-date pub-type="ppub"><day>13</day><month>5</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>10</day><month>3</month><year>2016</year></pub-date><volume>291</volume><issue>20</issue><fpage>10490</fpage><lpage>10500</lpage><history><date date-type="received"><day>3</day><month>8</month><year>2015</year></date><date date-type="rev-recd"><day>7</day><month>3</month><year>2016</year></date></history><permissions><copyright-statement>© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.</copyright-statement><copyright-year>2016</copyright-year><copyright-holder>The American Society for Biochemistry and Molecular Biology, Inc.</copyright-holder></permissions><self-uri content-type="pdf" xlink:href="zbc02016010490.pdf"></self-uri><abstract><p>Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), a type I transmembrane glycoprotein, is known as one of the most specific lymphatic vessel markers in the skin. In this study, we found that the ectodomain of LYVE-1 undergoes proteolytic cleavage, and this process produces soluble LYVE-1. We further identified the cleavage site for ectodomain shedding and generated an uncleavable mutant of LYVE-1. In lymphatic endothelial cells, ectodomain shedding of LYVE-1 was induced by vascular endothelial growth factor (VEGF)-A, an important factor for angiogenesis and lymphangiogenesis under pathological conditions. VEGF-A-induced LYVE-1 ectodomain shedding was mediated via the extracellular signal-regulated kinase (ERK) and a disintegrin and metalloproteinase (ADAM) 17. Wild-type LYVE-1, but not uncleavable LYVE-1, promoted migration of lymphatic endothelial cells in response to VEGF-A. Immunostaining analyses in human psoriasis skin lesions and VEGF-A transgenic mouse skin suggested that the ectodomain shedding of LYVE-1 occurred in lymphatic vessels undergoing chronic inflammation. These results indicate that the ectodomain shedding of LYVE-1 might be involved in promoting pathological lymphangiogenesis.</p></abstract><kwd-group><kwd>ADAM</kwd><kwd>endothelial cell</kwd><kwd>extracellular signal-regulated kinase (ERK)</kwd><kwd>hyaluronan</kwd><kwd>lymphangiogenesis</kwd><kwd>psoriasis</kwd><kwd>shedding</kwd><kwd>skin</kwd><kwd>vascular endothelial growth factor (VEGF)</kwd><kwd>lymphatic endothelial hyaluronan receptor 1 (LYVE-1)</kwd></kwd-group></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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