In vivo determination of collecting lymphatic vessel permeability to albumin: a role for lymphatics in exchange
Identifieur interne : 003842 ( Pmc/Checkpoint ); précédent : 003841; suivant : 003843In vivo determination of collecting lymphatic vessel permeability to albumin: a role for lymphatics in exchange
Auteurs : Joshua P. Scallan ; Virginia H. HuxleySource :
- The Journal of Physiology [ 0022-3751 ] ; 2009.
Abstract
While it is well established that the lymphatic vasculature is central to fluid and solute homeostasis, how it accomplishes this task is not well defined. To clarify the basic mechanisms underlying basal fluid and solute homeostasis, we assessed permeability to rat serum albumin (
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DOI: 10.1113/jphysiol.2009.179622
PubMed: 19917564
PubMed Central: 2821562
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determination of collecting lymphatic vessel permeability to albumin: a role for lymphatics in exchange</title>
<author><name sortKey="Scallan, Joshua P" sort="Scallan, Joshua P" uniqKey="Scallan J" first="Joshua P" last="Scallan">Joshua P. Scallan</name>
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<author><name sortKey="Huxley, Virginia H" sort="Huxley, Virginia H" uniqKey="Huxley V" first="Virginia H" last="Huxley">Virginia H. Huxley</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>In vivo</italic>
determination of collecting lymphatic vessel permeability to albumin: a role for lymphatics in exchange</title>
<author><name sortKey="Scallan, Joshua P" sort="Scallan, Joshua P" uniqKey="Scallan J" first="Joshua P" last="Scallan">Joshua P. Scallan</name>
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<series><title level="j">The Journal of Physiology</title>
<idno type="ISSN">0022-3751</idno>
<idno type="eISSN">1469-7793</idno>
<imprint><date when="2009">2009</date>
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<front><div type="abstract" xml:lang="en"><p>While it is well established that the lymphatic vasculature is central to fluid and solute homeostasis, how it accomplishes this task is not well defined. To clarify the basic mechanisms underlying basal fluid and solute homeostasis, we assessed permeability to rat serum albumin (<inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
) in mesenteric collecting lymphatic vessels and venules of juvenile male rats. Using the quantitative microfluorometric technique originally developed for blood capillaries, we tested the hypothesis that as a consequence of venules and collecting lymphatics sharing a common embryological origin, their <inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
would not differ significantly. Supporting our hypothesis, the median collecting lymphatic <inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
(3.5 ± 1.0 × 10<sup>−7</sup>
cm s<sup>−1</sup>
, <italic>N</italic>
= 22) did not differ significantly from the median venular <inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
(4.0 ± 1.0 × 10<sup>−7</sup>
cm s<sup>−1</sup>
, <italic>N</italic>
= 8, <italic>P</italic>
= 0.61). For collecting lymphatics the diffusive permeability (<italic>P</italic>
<sub>d</sub>
= 2.5 × 10<sup>−7</sup>
cm s<sup>−1</sup>
) was obtained from the relationship of apparent <inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
and pressure. While the measured <inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
, <italic>P</italic>
<sub>d</sub>
and estimated hydraulic conductivity of collecting lymphatics and venules were similar, the contribution of convective coupling differs as a result of the higher hydrostatic pressure experienced by venules relative to collecting lymphatics <italic>in vivo</italic>
. In summary, the data demonstrate the capacity for collecting lymphatics to act as exchange vessels, able to extravasate solute and filter fluid. As a consequence these data provide experimental support for the theory that prenodal lymphatic vessels concentrate intraluminal protein.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">J Physiol</journal-id>
<journal-id journal-id-type="publisher-id">tjp</journal-id>
<journal-title-group><journal-title>The Journal of Physiology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0022-3751</issn>
<issn pub-type="epub">1469-7793</issn>
<publisher><publisher-name>Blackwell Science Inc</publisher-name>
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<article-meta><article-id pub-id-type="pmid">19917564</article-id>
<article-id pub-id-type="pmc">2821562</article-id>
<article-id pub-id-type="doi">10.1113/jphysiol.2009.179622</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Cardiovascular</subject>
</subj-group>
</article-categories>
<title-group><article-title><italic>In vivo</italic>
determination of collecting lymphatic vessel permeability to albumin: a role for lymphatics in exchange</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Scallan</surname>
<given-names>Joshua P</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Huxley</surname>
<given-names>Virginia H</given-names>
</name>
</contrib>
<aff><institution>Department of Medical Pharmacology & Physiology, University of Missouri School of Medicine</institution>
<addr-line>Columbia, MO, USA</addr-line>
</aff>
</contrib-group>
<author-notes><corresp id="cor1"><bold>Corresponding author</bold>
V. H. Huxley: Department of Medical Pharmacology & Physiology, University of Missouri School of Medicine, 1 Hospital Dr., MA415, Columbia, MO 65212, USA. Email: <email>huxleyv@health.missouri.edu</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><day>01</day>
<month>1</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="epub"><day>16</day>
<month>11</month>
<year>2009</year>
</pub-date>
<volume>588</volume>
<issue>Pt 1</issue>
<fpage>243</fpage>
<lpage>254</lpage>
<history><date date-type="received"><day>30</day>
<month>7</month>
<year>2009</year>
</date>
<date date-type="accepted"><day>09</day>
<month>11</month>
<year>2009</year>
</date>
</history>
<permissions><copyright-statement>Journal compilation © 2010 The Physiological Society</copyright-statement>
</permissions>
<abstract><p>While it is well established that the lymphatic vasculature is central to fluid and solute homeostasis, how it accomplishes this task is not well defined. To clarify the basic mechanisms underlying basal fluid and solute homeostasis, we assessed permeability to rat serum albumin (<inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
) in mesenteric collecting lymphatic vessels and venules of juvenile male rats. Using the quantitative microfluorometric technique originally developed for blood capillaries, we tested the hypothesis that as a consequence of venules and collecting lymphatics sharing a common embryological origin, their <inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
would not differ significantly. Supporting our hypothesis, the median collecting lymphatic <inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
(3.5 ± 1.0 × 10<sup>−7</sup>
cm s<sup>−1</sup>
, <italic>N</italic>
= 22) did not differ significantly from the median venular <inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
(4.0 ± 1.0 × 10<sup>−7</sup>
cm s<sup>−1</sup>
, <italic>N</italic>
= 8, <italic>P</italic>
= 0.61). For collecting lymphatics the diffusive permeability (<italic>P</italic>
<sub>d</sub>
= 2.5 × 10<sup>−7</sup>
cm s<sup>−1</sup>
) was obtained from the relationship of apparent <inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
and pressure. While the measured <inline-formula><inline-graphic xlink:href="tjp0588-0243-mu1.jpg" mimetype="image"></inline-graphic>
</inline-formula>
, <italic>P</italic>
<sub>d</sub>
and estimated hydraulic conductivity of collecting lymphatics and venules were similar, the contribution of convective coupling differs as a result of the higher hydrostatic pressure experienced by venules relative to collecting lymphatics <italic>in vivo</italic>
. In summary, the data demonstrate the capacity for collecting lymphatics to act as exchange vessels, able to extravasate solute and filter fluid. As a consequence these data provide experimental support for the theory that prenodal lymphatic vessels concentrate intraluminal protein.</p>
</abstract>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Huxley, Virginia H" sort="Huxley, Virginia H" uniqKey="Huxley V" first="Virginia H" last="Huxley">Virginia H. Huxley</name>
<name sortKey="Scallan, Joshua P" sort="Scallan, Joshua P" uniqKey="Scallan J" first="Joshua P" last="Scallan">Joshua P. Scallan</name>
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