Effects of Adipose-derived Stromal Cells and of their Extract on Wound Healing in a Mouse Model
Identifieur interne : 003442 ( Pmc/Checkpoint ); précédent : 003441; suivant : 003443Effects of Adipose-derived Stromal Cells and of their Extract on Wound Healing in a Mouse Model
Auteurs : Jin Soo Lim [Corée du Sud] ; Gyeol Yoo [Corée du Sud]Source :
- Journal of Korean Medical Science [ 1011-8934 ] ; 2010.
Abstract
In this study, the authors investigated the effects of adipose-derived stromal cells (ADSCs) and of their extract on wound healing. After creating wound healing splint model on the backs of mice, ADSCs and their extract were applied. Wound healing rates were calculated at 3, 5, 7, 10, and 14 days after the wounding, and tissues were harvested at 7 and 14 days for histological analysis. Wound healing rates were significantly higher at 7, 10, and 14 days in the cell group than in the control, but in the cell extract group wound healing rates were significantly decreased (
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DOI: 10.3346/jkms.2010.25.5.746
PubMed: 20436712
PubMed Central: 2858835
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of Adipose-derived Stromal Cells and of their Extract on Wound Healing in a Mouse Model</title><author><name sortKey="Lim, Jin Soo" sort="Lim, Jin Soo" uniqKey="Lim J" first="Jin Soo" last="Lim">Jin Soo Lim</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Yoo, Gyeol" sort="Yoo, Gyeol" uniqKey="Yoo G" first="Gyeol" last="Yoo">Gyeol Yoo</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20436712</idno><idno type="pmc">2858835</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858835</idno><idno type="RBID">PMC:2858835</idno><idno type="doi">10.3346/jkms.2010.25.5.746</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">004088</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">004088</idno><idno type="wicri:Area/Pmc/Curation">004087</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">004087</idno><idno type="wicri:Area/Pmc/Checkpoint">003442</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003442</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Effects of Adipose-derived Stromal Cells and of their Extract on Wound Healing in a Mouse Model</title><author><name sortKey="Lim, Jin Soo" sort="Lim, Jin Soo" uniqKey="Lim J" first="Jin Soo" last="Lim">Jin Soo Lim</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Yoo, Gyeol" sort="Yoo, Gyeol" uniqKey="Yoo G" first="Gyeol" last="Yoo">Gyeol Yoo</name><affiliation wicri:level="3"><nlm:aff id="A1">Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.</nlm:aff><country xml:lang="fr" wicri:curation="lc">Corée du Sud</country><wicri:regionArea>Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Journal of Korean Medical Science</title><idno type="ISSN">1011-8934</idno><idno type="eISSN">1598-6357</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>In this study, the authors investigated the effects of adipose-derived stromal cells (ADSCs) and of their extract on wound healing. After creating wound healing splint model on the backs of mice, ADSCs and their extract were applied. Wound healing rates were calculated at 3, 5, 7, 10, and 14 days after the wounding, and tissues were harvested at 7 and 14 days for histological analysis. Wound healing rates were significantly higher at 7, 10, and 14 days in the cell group than in the control, but in the cell extract group wound healing rates were significantly decreased (<italic>P</italic><0.05). Histological scores and capillary densities in the cell group were significantly higher at 2 weeks (<italic>P</italic><0.05). In the cell group, thick inflammatory cell infiltration and many capillaries were observed at 1 week, and thick epithelium and numerous large capillaries were observed at 2 weeks. The present study suggests that ADSCs accelerate wound healing as known, and the effects of ADSCs on wound healing may be due to replacing insufficient cells by differentiation of ADSCs in the wound and secreting growth factors by differentiated cells, and not due to the effect of factors within ADSCs.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Martin, P" uniqKey="Martin P">P Martin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Falanga, V" uniqKey="Falanga V">V Falanga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zuk, Pa" uniqKey="Zuk P">PA Zuk</name></author><author><name sortKey="Zhu, M" uniqKey="Zhu M">M Zhu</name></author><author><name sortKey="Ashjian, P" uniqKey="Ashjian P">P Ashjian</name></author><author><name sortKey="De Ugarte, Da" uniqKey="De Ugarte D">DA De Ugarte</name></author><author><name sortKey="Huang, Ji" uniqKey="Huang J">JI 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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Korean Med Sci</journal-id><journal-id journal-id-type="publisher-id">JKMS</journal-id><journal-title-group><journal-title>Journal of Korean Medical Science</journal-title></journal-title-group><issn pub-type="ppub">1011-8934</issn><issn pub-type="epub">1598-6357</issn><publisher><publisher-name>The Korean Academy of Medical Sciences</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">20436712</article-id><article-id pub-id-type="pmc">2858835</article-id><article-id pub-id-type="doi">10.3346/jkms.2010.25.5.746</article-id><article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject><subj-group><subject>Surgery</subject></subj-group></subj-group></article-categories><title-group><article-title>Effects of Adipose-derived Stromal Cells and of their Extract on Wound Healing in a Mouse Model</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lim</surname><given-names>Jin Soo</given-names></name><xref ref-type="aff" rid="A1"></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Yoo</surname><given-names>Gyeol</given-names></name><xref ref-type="aff" rid="A1"></xref></contrib></contrib-group><aff id="A1">Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Seoul, Korea.</aff><author-notes><corresp>Address for correspondence: Gyeol Yoo, M.D. Department of Plastic Surgery, College of Medicine, The Catholic University of Korea, Yeouido St. Mary's Hospital, 49 Yuksam-gil, Yeongdeungpo-gu, Seoul 150-713, Korea. Tel: +82.2-3779-1198, Fax: +82.2-780-9167, <email>psyg@catholic.ac.kr</email></corresp></author-notes><pub-date pub-type="ppub"><month>5</month><year>2010</year></pub-date><pub-date pub-type="epub"><day>22</day><month>4</month><year>2010</year></pub-date><volume>25</volume><issue>5</issue><fpage>746</fpage><lpage>751</lpage><history><date date-type="received"><day>07</day><month>5</month><year>2009</year></date><date date-type="accepted"><day>24</day><month>9</month><year>2009</year></date></history><permissions><copyright-statement>© 2010 The Korean Academy of Medical Sciences.</copyright-statement><copyright-year>2010</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc/3.0"><license-p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/3.0">http://creativecommons.org/licenses/by-nc/3.0</ext-link>) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p>In this study, the authors investigated the effects of adipose-derived stromal cells (ADSCs) and of their extract on wound healing. After creating wound healing splint model on the backs of mice, ADSCs and their extract were applied. Wound healing rates were calculated at 3, 5, 7, 10, and 14 days after the wounding, and tissues were harvested at 7 and 14 days for histological analysis. Wound healing rates were significantly higher at 7, 10, and 14 days in the cell group than in the control, but in the cell extract group wound healing rates were significantly decreased (<italic>P</italic><0.05). Histological scores and capillary densities in the cell group were significantly higher at 2 weeks (<italic>P</italic><0.05). In the cell group, thick inflammatory cell infiltration and many capillaries were observed at 1 week, and thick epithelium and numerous large capillaries were observed at 2 weeks. The present study suggests that ADSCs accelerate wound healing as known, and the effects of ADSCs on wound healing may be due to replacing insufficient cells by differentiation of ADSCs in the wound and secreting growth factors by differentiated cells, and not due to the effect of factors within ADSCs.</p></abstract><kwd-group><kwd>Adipose-derived Stromal Cell</kwd><kwd>Cell Extract</kwd><kwd>Wound Healing</kwd></kwd-group></article-meta></front><floats-group><fig id="F1" position="float"><label>Fig. 1</label><caption><p>Two 6-mm circular full-thickness skin defects on the back of mouse with silicone rings.</p></caption><graphic xlink:href="jkms-25-746-g001"></graphic></fig><fig id="F2" position="float"><label>Fig. 2</label><caption><p>Changes of wound healing rates of the control, the cell, and the cell extract groups at 3, 5, 7, 10, and 14 days after wounding.</p><p><sup>*</sup><italic>P</italic><0.05 compared to control; <sup>†</sup><italic>P</italic><0.05 compared to the cell group; <sup>‡</sup><italic>P</italic><0.05 compared to the cell extract group.</p></caption><graphic xlink:href="jkms-25-746-g002"></graphic></fig><fig id="F3" position="float"><label>Fig. 3</label><caption><p>Histological scores of wound in the control, the cell group, and the cell extract groups at 1 and 2 weeks after wounding.</p><p><sup>*</sup><italic>P</italic><0.05 compared to control and the cell extract group.</p></caption><graphic xlink:href="jkms-25-746-g003"></graphic></fig><fig id="F4" position="float"><label>Fig. 4</label><caption><p>Micrographs of wound bed and wound margin in each group at 1 and 2 weeks after wounding (H&E stain, 40). Wound edges are indicated by arrows. At 1 week after wounding, the control group shows thin granulation tissue containing many neutrophils and lymphocytes (<bold>A</bold>), the cell group shows thick inflammatory cell infiltrations with many capillaries (<bold>B</bold>), and the cell extract group has more granulation tissues than in the control group (<bold>C</bold>). At 2 weeks after wounding, the control group shows thick granulation tissues with little re-epithelialization (<bold>D</bold>), the cell group shows slightly thicker epithelium and greater granulation tissue (<bold>E</bold>), and the cell extract group shows less re-epithelization and granulation tissue than in the cell group at 2 weeks (<bold>F</bold>).</p></caption><graphic xlink:href="jkms-25-746-g004"></graphic></fig><fig id="F5" position="float"><label>Fig. 5</label><caption><p>Micrographs of wound bed and wound margin in each group at 1 and 2 weeks after wounding (Immunohistochemical staining for CD31, ×100). Capillaries with endothelial cells are stained brown (thin arrows). Wound edges are indicated by thick arrows. At 1 week, some small numbers of capillaries are observed in granulation tissues in the control group (<bold>A</bold>) and the cell extract group (<bold>C</bold>), but large numbers of capillaries are observed in the cell group (<bold>B</bold>). At 2 weeks after wounding, increased capillary development is noted in the control group (<bold>D</bold>), the cell group (<bold>E</bold>), and the cell extract groups (<bold>F</bold>). Particularly, in the cell group numerous larger capillaries are observed in comparison with other groups.</p></caption><graphic xlink:href="jkms-25-746-g005"></graphic></fig><fig id="F6" position="float"><label>Fig. 6</label><caption><p>Capillary densities in wounds of the control, cell group, and cell extract group at 1 and 2 weeks after wounding.</p><p><sup>*</sup><italic>P</italic><0.05 compared to the control and cell extract groups.</p></caption><graphic xlink:href="jkms-25-746-g006"></graphic></fig></floats-group></pmc><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Lim, Jin Soo" sort="Lim, Jin Soo" uniqKey="Lim J" first="Jin Soo" last="Lim">Jin Soo Lim</name></region><name sortKey="Yoo, Gyeol" sort="Yoo, Gyeol" uniqKey="Yoo G" first="Gyeol" last="Yoo">Gyeol Yoo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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