Vascular endothelial growth factor-C enhances radiosensitivity of lymphatic endothelial cells
Identifieur interne : 001E63 ( Pmc/Checkpoint ); précédent : 001E62; suivant : 001E64Vascular endothelial growth factor-C enhances radiosensitivity of lymphatic endothelial cells
Auteurs : Cristina T. Kesler [États-Unis] ; Angera Kuo [États-Unis] ; Hon-Kit Wong [États-Unis] ; David J. Masuck [États-Unis] ; Jennifer L. Shah [États-Unis] ; Kevin Kozak [États-Unis] ; Kathryn D. Held [États-Unis] ; Timothy P. Padera [États-Unis]Source :
- Angiogenesis [ 0969-6970 ] ; 2013.
Abstract
Radiation therapy after lymph node dissection increases the risk of developing painful and incurable lymphedema in breast cancer patients. Lymphedema occurs when lymphatic vessels become unable to maintain proper fluid balance. The sensitivity of lymphatic endothelial cells (LECs) to ionizing radiation has not been reported to date. Here, the radiosensitivity of LECs
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DOI: 10.1007/s10456-013-9400-7
PubMed: 24201897
PubMed Central: 3981926
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Kesler</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author><author><name sortKey="Kuo, Angera" sort="Kuo, Angera" uniqKey="Kuo A" first="Angera" last="Kuo">Angera Kuo</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Hon Kit" sort="Wong, Hon Kit" uniqKey="Wong H" first="Hon-Kit" last="Wong">Hon-Kit Wong</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author><author><name sortKey="Masuck, David J" sort="Masuck, David J" uniqKey="Masuck D" first="David J." last="Masuck">David J. 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Kesler</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author><author><name sortKey="Kuo, Angera" sort="Kuo, Angera" uniqKey="Kuo A" first="Angera" last="Kuo">Angera Kuo</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Hon Kit" sort="Wong, Hon Kit" uniqKey="Wong H" first="Hon-Kit" last="Wong">Hon-Kit Wong</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author><author><name sortKey="Masuck, David J" sort="Masuck, David J" uniqKey="Masuck D" first="David J." last="Masuck">David J. Masuck</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author><author><name sortKey="Shah, Jennifer L" sort="Shah, Jennifer L" uniqKey="Shah J" first="Jennifer L." last="Shah">Jennifer L. Shah</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author><author><name sortKey="Kozak, Kevin" sort="Kozak, Kevin" uniqKey="Kozak K" first="Kevin" last="Kozak">Kevin Kozak</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author><author><name sortKey="Held, Kathryn D" sort="Held, Kathryn D" uniqKey="Held K" first="Kathryn D." last="Held">Kathryn D. Held</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author><author><name sortKey="Padera, Timothy P" sort="Padera, Timothy P" uniqKey="Padera T" first="Timothy P." last="Padera">Timothy P. Padera</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA</wicri:regionArea><wicri:noRegion>MA</wicri:noRegion></affiliation></author></analytic><series><title level="j">Angiogenesis</title><idno type="ISSN">0969-6970</idno><idno type="eISSN">1573-7209</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Radiation therapy after lymph node dissection increases the risk of developing painful and incurable lymphedema in breast cancer patients. Lymphedema occurs when lymphatic vessels become unable to maintain proper fluid balance. The sensitivity of lymphatic endothelial cells (LECs) to ionizing radiation has not been reported to date. Here, the radiosensitivity of LECs <italic>in vitro</italic> has been determined using clonogenic survival assays. The ability of various growth factors to alter LEC radiosensitivity was also examined. Vascular endothelial growth factor (VEGF)-C enhanced radiosensitivity when LECs were treated prior to radiation. VEGF-C-treated LECs exhibited higher levels of entry into the cell cycle at the time of radiation, with a greater number of cells in the S and G2/M phases. These LECs showed higher levels of H2A.X—an indicator of DNA damage—after radiation. VEGF-C did not increase cell death as a result of radiation. Instead, it increased the relative number of quiescent LECs. These data suggest that abundant VEGF-C or lymphangiogenesis may predispose patients to radiation-induced lymphedema by impairing lymphatic vessel repair through induction of LEC quiescence.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9814575</journal-id><journal-id journal-id-type="pubmed-jr-id">22063</journal-id><journal-id journal-id-type="nlm-ta">Angiogenesis</journal-id><journal-id journal-id-type="iso-abbrev">Angiogenesis</journal-id><journal-title-group><journal-title>Angiogenesis</journal-title></journal-title-group><issn pub-type="ppub">0969-6970</issn><issn pub-type="epub">1573-7209</issn></journal-meta><article-meta><article-id pub-id-type="pmid">24201897</article-id><article-id pub-id-type="pmc">3981926</article-id><article-id pub-id-type="doi">10.1007/s10456-013-9400-7</article-id><article-id pub-id-type="manuscript">NIHMS538992</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Vascular endothelial growth factor-C enhances radiosensitivity of lymphatic endothelial cells</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kesler</surname><given-names>Cristina T.</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref rid="FN6" ref-type="author-notes">*</xref></contrib><contrib contrib-type="author"><name><surname>Kuo</surname><given-names>Angera</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref rid="FN2" ref-type="author-notes">2</xref><xref rid="FN6" ref-type="author-notes">*</xref></contrib><contrib contrib-type="author"><name><surname>Wong</surname><given-names>Hon-Kit</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref rid="FN3" ref-type="author-notes">3</xref></contrib><contrib contrib-type="author"><name><surname>Masuck</surname><given-names>David J.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Shah</surname><given-names>Jennifer L.</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref rid="FN4" ref-type="author-notes">4</xref></contrib><contrib contrib-type="author"><name><surname>Kozak</surname><given-names>Kevin</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref rid="FN5" ref-type="author-notes">5</xref></contrib><contrib contrib-type="author"><name><surname>Held</surname><given-names>Kathryn D.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Padera</surname><given-names>Timothy P.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib></contrib-group><aff id="A1"><label>1</label>Department of Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA</aff><author-notes><corresp id="FN1">Corresponding Author (to whom all correspondence should be sent): Timothy P. Padera, PhD, Massachusetts General Hospital, Department of Radiation Oncology, Cox-7, 100 Blossom St., Boston, MA 02114, USA, Phone: 617-726-8143, Fax: 617-724-5841, <email>tpadera@steele.mgh.harvard.edu</email></corresp><fn id="FN2" fn-type="current-aff"><label>2</label><p>Current Affiliation: Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA</p></fn><fn id="FN3" fn-type="current-aff"><label>3</label><p>Current Affiliation: Department of Neurology, Brigham and Women’s Hospital and Harvard Medical School, Boston, MA, USA</p></fn><fn id="FN4" fn-type="current-aff"><label>4</label><p>Current Affiliation: Department of Radiation Oncology, Stanford University School of Medicine, Palo Alto, CA, USA</p></fn><fn id="FN5" fn-type="current-aff"><label>5</label><p>Current Affiliation: Department of Human Oncology, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA</p></fn><fn id="FN6" fn-type="equal"><label>*</label><p>These authors contributed equally to this work.</p></fn></author-notes><pub-date pub-type="nihms-submitted"><day>17</day><month>12</month><year>2013</year></pub-date><pub-date pub-type="epub"><day>08</day><month>11</month><year>2013</year></pub-date><pub-date pub-type="ppub"><month>4</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>01</day><month>4</month><year>2015</year></pub-date><volume>17</volume><issue>2</issue><fpage>419</fpage><lpage>427</lpage><pmc-comment>elocation-id from pubmed: 10.1007/s10456-013-9400-7</pmc-comment>
<abstract><p id="P1">Radiation therapy after lymph node dissection increases the risk of developing painful and incurable lymphedema in breast cancer patients. Lymphedema occurs when lymphatic vessels become unable to maintain proper fluid balance. The sensitivity of lymphatic endothelial cells (LECs) to ionizing radiation has not been reported to date. Here, the radiosensitivity of LECs <italic>in vitro</italic> has been determined using clonogenic survival assays. The ability of various growth factors to alter LEC radiosensitivity was also examined. Vascular endothelial growth factor (VEGF)-C enhanced radiosensitivity when LECs were treated prior to radiation. VEGF-C-treated LECs exhibited higher levels of entry into the cell cycle at the time of radiation, with a greater number of cells in the S and G2/M phases. These LECs showed higher levels of H2A.X—an indicator of DNA damage—after radiation. VEGF-C did not increase cell death as a result of radiation. Instead, it increased the relative number of quiescent LECs. These data suggest that abundant VEGF-C or lymphangiogenesis may predispose patients to radiation-induced lymphedema by impairing lymphatic vessel repair through induction of LEC quiescence.</p></abstract><kwd-group><kwd>lymphedema</kwd><kwd>lymphangiogenesis</kwd><kwd>radiosensitivity</kwd><kwd>lymphatic endothelium</kwd><kwd>VEGF-C</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Kesler, Cristina T" sort="Kesler, Cristina T" uniqKey="Kesler C" first="Cristina T." last="Kesler">Cristina T. Kesler</name></noRegion><name sortKey="Held, Kathryn D" sort="Held, Kathryn D" uniqKey="Held K" first="Kathryn D." last="Held">Kathryn D. Held</name><name sortKey="Kozak, Kevin" sort="Kozak, Kevin" uniqKey="Kozak K" first="Kevin" last="Kozak">Kevin Kozak</name><name sortKey="Kuo, Angera" sort="Kuo, Angera" uniqKey="Kuo A" first="Angera" last="Kuo">Angera Kuo</name><name sortKey="Masuck, David J" sort="Masuck, David J" uniqKey="Masuck D" first="David J." last="Masuck">David J. Masuck</name><name sortKey="Padera, Timothy P" sort="Padera, Timothy P" uniqKey="Padera T" first="Timothy P." last="Padera">Timothy P. Padera</name><name sortKey="Shah, Jennifer L" sort="Shah, Jennifer L" uniqKey="Shah J" first="Jennifer L." last="Shah">Jennifer L. Shah</name><name sortKey="Wong, Hon Kit" sort="Wong, Hon Kit" uniqKey="Wong H" first="Hon-Kit" last="Wong">Hon-Kit Wong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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