CLEC-2 is required for development and maintenance of lymph nodes
Identifieur interne : 001D02 ( Pmc/Checkpoint ); précédent : 001D01; suivant : 001D03CLEC-2 is required for development and maintenance of lymph nodes
Auteurs : Cécile Bénézech ; Saba Nayar ; Brenda A. Finney [Royaume-Uni] ; David R. Withers ; Kate Lowe [Royaume-Uni] ; Guillaume E. Desanti ; Clare L. Marriott ; Steve P. Watson [Royaume-Uni] ; Jorge H. Caama O ; Christopher D. Buckley ; Francesca BaroneSource :
- Blood [ 0006-4971 ] ; 2014.
Abstract
CLEC-2 is necessary for lymphatic cell proliferation and lymph node anlage persistence after birth. Lack of CLEC-2 expression on megakaryocytes and platelets compromises lymph node integrity in adult life.
Url:
DOI: 10.1182/blood-2013-03-489286
PubMed: 24532804
PubMed Central: 4023425
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
PMC:4023425Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">CLEC-2 is required for development and maintenance of lymph nodes</title><author><name sortKey="Benezech, Cecile" sort="Benezech, Cecile" uniqKey="Benezech C" first="Cécile" last="Bénézech">Cécile Bénézech</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Nayar, Saba" sort="Nayar, Saba" uniqKey="Nayar S" first="Saba" last="Nayar">Saba Nayar</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Finney, Brenda A" sort="Finney, Brenda A" uniqKey="Finney B" first="Brenda A." last="Finney">Brenda A. Finney</name><affiliation wicri:level="4"><nlm:aff id="aff2">Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham</wicri:regionArea><placeName><settlement type="city">Birmingham</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Midlands de l'Ouest</region><settlement type="city">Birmingham</settlement></placeName><orgName type="university">Université de Birmingham</orgName></affiliation></author><author><name sortKey="Withers, David R" sort="Withers, David R" uniqKey="Withers D" first="David R." last="Withers">David R. Withers</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Lowe, Kate" sort="Lowe, Kate" uniqKey="Lowe K" first="Kate" last="Lowe">Kate Lowe</name><affiliation wicri:level="4"><nlm:aff id="aff2">Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham</wicri:regionArea><placeName><settlement type="city">Birmingham</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Midlands de l'Ouest</region><settlement type="city">Birmingham</settlement></placeName><orgName type="university">Université de Birmingham</orgName></affiliation></author><author><name sortKey="Desanti, Guillaume E" sort="Desanti, Guillaume E" uniqKey="Desanti G" first="Guillaume E." last="Desanti">Guillaume E. Desanti</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Marriott, Clare L" sort="Marriott, Clare L" uniqKey="Marriott C" first="Clare L." last="Marriott">Clare L. Marriott</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Watson, Steve P" sort="Watson, Steve P" uniqKey="Watson S" first="Steve P." last="Watson">Steve P. Watson</name><affiliation wicri:level="4"><nlm:aff id="aff2">Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham</wicri:regionArea><placeName><settlement type="city">Birmingham</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Midlands de l'Ouest</region><settlement type="city">Birmingham</settlement></placeName><orgName type="university">Université de Birmingham</orgName></affiliation></author><author><name sortKey="Caama O, Jorge H" sort="Caama O, Jorge H" uniqKey="Caama O J" first="Jorge H." last="Caama O">Jorge H. Caama O</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Buckley, Christopher D" sort="Buckley, Christopher D" uniqKey="Buckley C" first="Christopher D." last="Buckley">Christopher D. Buckley</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Barone, Francesca" sort="Barone, Francesca" uniqKey="Barone F" first="Francesca" last="Barone">Francesca Barone</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24532804</idno><idno type="pmc">4023425</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4023425</idno><idno type="RBID">PMC:4023425</idno><idno type="doi">10.1182/blood-2013-03-489286</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">002C83</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">002C83</idno><idno type="wicri:Area/Pmc/Curation">002C82</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">002C82</idno><idno type="wicri:Area/Pmc/Checkpoint">001D02</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001D02</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">CLEC-2 is required for development and maintenance of lymph nodes</title><author><name sortKey="Benezech, Cecile" sort="Benezech, Cecile" uniqKey="Benezech C" first="Cécile" last="Bénézech">Cécile Bénézech</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Nayar, Saba" sort="Nayar, Saba" uniqKey="Nayar S" first="Saba" last="Nayar">Saba Nayar</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Finney, Brenda A" sort="Finney, Brenda A" uniqKey="Finney B" first="Brenda A." last="Finney">Brenda A. Finney</name><affiliation wicri:level="4"><nlm:aff id="aff2">Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham</wicri:regionArea><placeName><settlement type="city">Birmingham</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Midlands de l'Ouest</region><settlement type="city">Birmingham</settlement></placeName><orgName type="university">Université de Birmingham</orgName></affiliation></author><author><name sortKey="Withers, David R" sort="Withers, David R" uniqKey="Withers D" first="David R." last="Withers">David R. Withers</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Lowe, Kate" sort="Lowe, Kate" uniqKey="Lowe K" first="Kate" last="Lowe">Kate Lowe</name><affiliation wicri:level="4"><nlm:aff id="aff2">Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham</wicri:regionArea><placeName><settlement type="city">Birmingham</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Midlands de l'Ouest</region><settlement type="city">Birmingham</settlement></placeName><orgName type="university">Université de Birmingham</orgName></affiliation></author><author><name sortKey="Desanti, Guillaume E" sort="Desanti, Guillaume E" uniqKey="Desanti G" first="Guillaume E." last="Desanti">Guillaume E. Desanti</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Marriott, Clare L" sort="Marriott, Clare L" uniqKey="Marriott C" first="Clare L." last="Marriott">Clare L. Marriott</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Watson, Steve P" sort="Watson, Steve P" uniqKey="Watson S" first="Steve P." last="Watson">Steve P. Watson</name><affiliation wicri:level="4"><nlm:aff id="aff2">Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom</nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham</wicri:regionArea><placeName><settlement type="city">Birmingham</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Midlands de l'Ouest</region><settlement type="city">Birmingham</settlement></placeName><orgName type="university">Université de Birmingham</orgName></affiliation></author><author><name sortKey="Caama O, Jorge H" sort="Caama O, Jorge H" uniqKey="Caama O J" first="Jorge H." last="Caama O">Jorge H. Caama O</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Buckley, Christopher D" sort="Buckley, Christopher D" uniqKey="Buckley C" first="Christopher D." last="Buckley">Christopher D. Buckley</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author><author><name sortKey="Barone, Francesca" sort="Barone, Francesca" uniqKey="Barone F" first="Francesca" last="Barone">Francesca Barone</name><affiliation><nlm:aff wicri:cut=" and" id="aff1">School of Immunity and Infection</nlm:aff></affiliation></author></analytic><series><title level="j">Blood</title><idno type="ISSN">0006-4971</idno><idno type="eISSN">1528-0020</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Key Points</title><p><list list-type="bullet"><list-item><p>CLEC-2 is necessary for lymphatic cell proliferation and lymph node anlage persistence after birth.</p></list-item><list-item><p>Lack of CLEC-2 expression on megakaryocytes and platelets compromises lymph node integrity in adult life.</p></list-item></list></p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Blood</journal-id><journal-id journal-id-type="iso-abbrev">Blood</journal-id><journal-id journal-id-type="hwp">bloodjournal</journal-id><journal-id journal-id-type="pmc">blood</journal-id><journal-id journal-id-type="publisher-id">Blood</journal-id><journal-title-group><journal-title>Blood</journal-title></journal-title-group><issn pub-type="ppub">0006-4971</issn><issn pub-type="epub">1528-0020</issn><publisher><publisher-name>American Society of Hematology</publisher-name><publisher-loc>Washington, DC</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24532804</article-id><article-id pub-id-type="pmc">4023425</article-id><article-id pub-id-type="publisher-id">2013/489286</article-id><article-id pub-id-type="doi">10.1182/blood-2013-03-489286</article-id><article-categories><subj-group subj-group-type="hwp-journal-coll"><subject>12</subject><subject>38</subject></subj-group><subj-group subj-group-type="heading"><subject>Vascular Biology</subject></subj-group></article-categories><title-group><article-title>CLEC-2 is required for development and maintenance of lymph nodes</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Bénézech</surname><given-names>Cécile</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Nayar</surname><given-names>Saba</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Finney</surname><given-names>Brenda A.</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Withers</surname><given-names>David R.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Lowe</surname><given-names>Kate</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Desanti</surname><given-names>Guillaume E.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Marriott</surname><given-names>Clare L.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Watson</surname><given-names>Steve P.</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Caamaño</surname><given-names>Jorge H.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Buckley</surname><given-names>Christopher D.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Barone</surname><given-names>Francesca</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><aff id="aff1"><label>1</label>School of Immunity and Infection and</aff><aff id="aff2"><label>2</label>Centre for Cardiovascular Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom</aff></contrib-group><pub-date pub-type="ppub"><day>15</day><month>5</month><year>2014</year></pub-date><pub-date pub-type="epreprint"><day>14</day><month>2</month><year>2014</year></pub-date><pub-date pub-type="pmc-release"><day>15</day><month>5</month><year>2014</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
<volume>123</volume><issue>20</issue><fpage>3200</fpage><lpage>3207</lpage><history><date date-type="received"><day>12</day><month>3</month><year>2013</year></date><date date-type="accepted"><day>24</day><month>1</month><year>2014</year></date></history><permissions><copyright-statement>© 2014 by The American Society of Hematology</copyright-statement><copyright-year>2014</copyright-year></permissions><self-uri xlink:title="pdf" xlink:type="simple" xlink:href="3200.pdf"></self-uri><related-article related-article-type="article-reference" id="d35e182" vol="123" page="3057" issue="20" ext-link-type="pmc"></related-article><abstract abstract-type="executive-summary"><title>Key Points</title><p><list list-type="bullet"><list-item><p>CLEC-2 is necessary for lymphatic cell proliferation and lymph node anlage persistence after birth.</p></list-item><list-item><p>Lack of CLEC-2 expression on megakaryocytes and platelets compromises lymph node integrity in adult life.</p></list-item></list></p></abstract><abstract><p>The importance of CLEC-2, a natural ligand/receptor for Gp38/Podoplanin, in the formation of the lymphatic vasculature has recently been demonstrated. As the development and maintenance of lymph nodes (LNs) is dependent on the formation of the lymphatic vasculature and the differentiation of Gp38/Podoplanin<sup>+</sup> stromal cells, we investigated the role of CLEC-2 in lymphoneogenesis and LN homeostasis. Using constitutive <italic>Clec1b</italic><sup><italic>−/−</italic></sup> mice, we showed that while CLEC-2 was not necessary for initiation of the LN anlage, it was required at late stages of development. Constitutive deletion of CLEC-2 induced a profound defect in lymphatic endothelial cell proliferation, resulting in lack of LNs at birth. In contrast, conditional deletion of CLEC-2 in the megakaryocyte/platelet lineage in <italic>Clec1b<sup>fl/fl</sup>PF4-Cre</italic> mice led to the development of blood-filled LNs and fibrosis, in absence of a proliferative defect of the lymphatic endothelial compartment. This phenotype was also observed in chimeric mice reconstituted with <italic>Clec1b<sup>fl/fl</sup>PF4-Cre</italic> bone marrow, indicating that CLEC-2 expression in platelets was required for LN integrity. We demonstrated that LNs of <italic>Clec1b<sup>fl/fl</sup>PF4-Cre</italic> mice are able to sustain primary immune responses but show a defect in immune cell recirculation after repeated immunizations, thus suggesting CLEC-2 as target in chronic immune response.</p></abstract><counts><page-count count="8"></page-count></counts><custom-meta-group><custom-meta><meta-name>access</meta-name><meta-value>free</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Midlands de l'Ouest</li></region><settlement><li>Birmingham</li></settlement><orgName><li>Université de Birmingham</li></orgName></list><tree><noCountry><name sortKey="Barone, Francesca" sort="Barone, Francesca" uniqKey="Barone F" first="Francesca" last="Barone">Francesca Barone</name><name sortKey="Benezech, Cecile" sort="Benezech, Cecile" uniqKey="Benezech C" first="Cécile" last="Bénézech">Cécile Bénézech</name><name sortKey="Buckley, Christopher D" sort="Buckley, Christopher D" uniqKey="Buckley C" first="Christopher D." last="Buckley">Christopher D. Buckley</name><name sortKey="Caama O, Jorge H" sort="Caama O, Jorge H" uniqKey="Caama O J" first="Jorge H." last="Caama O">Jorge H. Caama O</name><name sortKey="Desanti, Guillaume E" sort="Desanti, Guillaume E" uniqKey="Desanti G" first="Guillaume E." last="Desanti">Guillaume E. Desanti</name><name sortKey="Marriott, Clare L" sort="Marriott, Clare L" uniqKey="Marriott C" first="Clare L." last="Marriott">Clare L. Marriott</name><name sortKey="Nayar, Saba" sort="Nayar, Saba" uniqKey="Nayar S" first="Saba" last="Nayar">Saba Nayar</name><name sortKey="Withers, David R" sort="Withers, David R" uniqKey="Withers D" first="David R." last="Withers">David R. Withers</name></noCountry><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Finney, Brenda A" sort="Finney, Brenda A" uniqKey="Finney B" first="Brenda A." last="Finney">Brenda A. Finney</name></region><name sortKey="Lowe, Kate" sort="Lowe, Kate" uniqKey="Lowe K" first="Kate" last="Lowe">Kate Lowe</name><name sortKey="Watson, Steve P" sort="Watson, Steve P" uniqKey="Watson S" first="Steve P." last="Watson">Steve P. Watson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Pmc/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001D02 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd -nk 001D02 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Pmc
|étape= Checkpoint
|type= RBID
|clé= PMC:4023425
|texte= CLEC-2 is required for development and maintenance of lymph nodes
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Checkpoint/RBID.i -Sk "pubmed:24532804" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |