A prospective pilot study of detection of sentinel lymph nodes in gynaecological cancers using a novel near infrared fluorescence imaging system
Identifieur interne : 001557 ( Pmc/Checkpoint ); précédent : 001556; suivant : 001558A prospective pilot study of detection of sentinel lymph nodes in gynaecological cancers using a novel near infrared fluorescence imaging system
Auteurs : Alexandros Laios [Royaume-Uni] ; Davide Volpi [Royaume-Uni] ; Iain D. C. Tullis [Royaume-Uni] ; Martha Woodward [Royaume-Uni] ; Stephen Kennedy [Royaume-Uni] ; Pubudu N. J. Pathiraja [Royaume-Uni] ; Krishnayan Haldar [Royaume-Uni] ; Borivoj Vojnovic [Royaume-Uni] ; Ahmed A. Ahmed [Royaume-Uni]Source :
- BMC Research Notes [ 1756-0500 ] ; 2015.
Abstract
Sentinel Lymph Node (SLN) sampling may significantly reduce surgical morbidity by avoiding needless radical lymphadenectomy. In gynaecological cancers, the current practice in the UK is testing the accuracy of SLN detection using radioactive isotopes within the context of clinical trials. However, radioactive tracers pose significant logistic problems. We, therefore, conducted a pilot, observational study to assess the feasibility of a novel optical imaging device for SLN detection in gynaecological cancers using near infrared (NIR) fluorescence.
A novel, custom-made, optical imaging system was developed to enable detection of multiple fluorescence dyes and allow simultaneous bright-field imaging during open surgery and laparoscopic procedures. We then evaluated the performance of the system in a prospective study of 49 women with early stage vulval, cervical and endometrial cancer who were scheduled to undergo complete lymphadenectomy. Clinically approved fluorescent contrast agents indocyanine green (ICG) and methylene blue (MB) were used. The main outcomes of the study included SLN mapping detection rates, false negative rates using the NIR fluorescence technique and safety of the procedures. We also examined the association between injection sites and differential lymphatic drainage in women with endometrial cancer by fluorescence imaging of ICG and MB.
A total of 64 SLNs were detected during both open surgery and laparoscopy. Following dose optimisation and the learning phase, SLN detection rate approached 100 % for all cancer types with no false negatives detected. Fluorescence from ICG and MB detected para-aortic SLNs in women with endometrial cancer following uterine injection. Percutaneous SLN detection was also achieved in most women with vulval cancer. No adverse reactions associated with the use of either dyes were observed.
This study demonstrated the successful clinical application of a novel NIR fluorescence imaging system for SLN detection across different gynaecological cancers. We showcased the first
The online version of this article (doi:10.1186/s13104-015-1576-z) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s13104-015-1576-z
PubMed: 26502876
PubMed Central: 4621870
Affiliations:
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<author><name sortKey="Kennedy, Stephen" sort="Kennedy, Stephen" uniqKey="Kennedy S" first="Stephen" last="Kennedy">Stephen Kennedy</name>
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<author><name sortKey="Pathiraja, Pubudu N J" sort="Pathiraja, Pubudu N J" uniqKey="Pathiraja P" first="Pubudu N. J." last="Pathiraja">Pubudu N. J. Pathiraja</name>
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<author><name sortKey="Vojnovic, Borivoj" sort="Vojnovic, Borivoj" uniqKey="Vojnovic B" first="Borivoj" last="Vojnovic">Borivoj Vojnovic</name>
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<wicri:regionArea>Department of Oncology, CRUK/MRC Oxford Institute for Radiation Oncology, University of Oxford, Oxford</wicri:regionArea>
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<author><name sortKey="Ahmed, Ahmed A" sort="Ahmed, Ahmed A" uniqKey="Ahmed A" first="Ahmed A." last="Ahmed">Ahmed A. Ahmed</name>
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<affiliation wicri:level="4"><nlm:aff id="Aff5">Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Headington, Oxford, OX37DS UK</nlm:aff>
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<front><div type="abstract" xml:lang="en"><sec><title>Background</title>
<p>Sentinel Lymph Node (SLN) sampling may significantly reduce surgical morbidity by avoiding needless radical lymphadenectomy. In gynaecological cancers, the current practice in the UK is testing the accuracy of SLN detection using radioactive isotopes within the context of clinical trials. However, radioactive tracers pose significant logistic problems. We, therefore, conducted a pilot, observational study to assess the feasibility of a novel optical imaging device for SLN detection in gynaecological cancers using near infrared (NIR) fluorescence.</p>
</sec>
<sec><title>Methods</title>
<p>A novel, custom-made, optical imaging system was developed to enable detection of multiple fluorescence dyes and allow simultaneous bright-field imaging during open surgery and laparoscopic procedures. We then evaluated the performance of the system in a prospective study of 49 women with early stage vulval, cervical and endometrial cancer who were scheduled to undergo complete lymphadenectomy. Clinically approved fluorescent contrast agents indocyanine green (ICG) and methylene blue (MB) were used. The main outcomes of the study included SLN mapping detection rates, false negative rates using the NIR fluorescence technique and safety of the procedures. We also examined the association between injection sites and differential lymphatic drainage in women with endometrial cancer by fluorescence imaging of ICG and MB.</p>
</sec>
<sec><title>Results</title>
<p>A total of 64 SLNs were detected during both open surgery and laparoscopy. Following dose optimisation and the learning phase, SLN detection rate approached 100 % for all cancer types with no false negatives detected. Fluorescence from ICG and MB detected para-aortic SLNs in women with endometrial cancer following uterine injection. Percutaneous SLN detection was also achieved in most women with vulval cancer. No adverse reactions associated with the use of either dyes were observed.</p>
</sec>
<sec><title>Conclusions</title>
<p>This study demonstrated the successful clinical application of a novel NIR fluorescence imaging system for SLN detection across different gynaecological cancers. We showcased the first <italic>in human</italic>
imaging, during the same procedure, of two fluorescence dyes in women with endometrial cancer.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s13104-015-1576-z) contains supplementary material, which is available to authorized users.</p>
</sec>
</div>
</front>
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</author>
<author><name sortKey="Guderian, D" uniqKey="Guderian D">D Guderian</name>
</author>
<author><name sortKey="Kohler, C" uniqKey="Kohler C">C Kohler</name>
</author>
<author><name sortKey="Schneider, A" uniqKey="Schneider A">A Schneider</name>
</author>
<author><name sortKey="Wendt, Tg" uniqKey="Wendt T">TG Wendt</name>
</author>
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<biblStruct><analytic><author><name sortKey="Walker, Jl" uniqKey="Walker J">JL Walker</name>
</author>
<author><name sortKey="Piedmonte, Mr" uniqKey="Piedmonte M">MR Piedmonte</name>
</author>
<author><name sortKey="Spirtos, Nm" uniqKey="Spirtos N">NM Spirtos</name>
</author>
<author><name sortKey="Eisenkop, Sm" uniqKey="Eisenkop S">SM Eisenkop</name>
</author>
<author><name sortKey="Schlaerth, Jb" uniqKey="Schlaerth J">JB Schlaerth</name>
</author>
<author><name sortKey="Mannel, Rs" uniqKey="Mannel R">RS Mannel</name>
</author>
<author><name sortKey="Spiegel, G" uniqKey="Spiegel G">G Spiegel</name>
</author>
<author><name sortKey="Barakat, R" uniqKey="Barakat R">R Barakat</name>
</author>
<author><name sortKey="Pearl, Ml" uniqKey="Pearl M">ML Pearl</name>
</author>
<author><name sortKey="Sharma, Sk" uniqKey="Sharma S">SK Sharma</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Oonk, Mh" uniqKey="Oonk M">MH Oonk</name>
</author>
<author><name sortKey="Van De Nieuwenhof, Hp" uniqKey="Van De Nieuwenhof H">HP van de Nieuwenhof</name>
</author>
<author><name sortKey="De Hullu, Ja" uniqKey="De Hullu J">JA de Hullu</name>
</author>
<author><name sortKey="Van Der Zee, Ag" uniqKey="Van Der Zee A">AG van der Zee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bostick, Pj" uniqKey="Bostick P">PJ Bostick</name>
</author>
<author><name sortKey="Giuliano, Ae" uniqKey="Giuliano A">AE Giuliano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mariani, G" uniqKey="Mariani G">G Mariani</name>
</author>
<author><name sortKey="Gipponi, M" uniqKey="Gipponi M">M Gipponi</name>
</author>
<author><name sortKey="Moresco, L" uniqKey="Moresco L">L Moresco</name>
</author>
<author><name sortKey="Villa, G" uniqKey="Villa G">G Villa</name>
</author>
<author><name sortKey="Bartolomei, M" uniqKey="Bartolomei M">M Bartolomei</name>
</author>
<author><name sortKey="Mazzarol, G" uniqKey="Mazzarol G">G Mazzarol</name>
</author>
<author><name sortKey="Bagnara, Mc" uniqKey="Bagnara M">MC Bagnara</name>
</author>
<author><name sortKey="Romanini, A" uniqKey="Romanini A">A Romanini</name>
</author>
<author><name sortKey="Cafiero, F" uniqKey="Cafiero F">F Cafiero</name>
</author>
<author><name sortKey="Paganelli, G" uniqKey="Paganelli G">G Paganelli</name>
</author>
<author><name sortKey="Strauss, Hw" uniqKey="Strauss H">HW Strauss</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meads, C" uniqKey="Meads C">C Meads</name>
</author>
<author><name sortKey="Sutton, Aj" uniqKey="Sutton A">AJ Sutton</name>
</author>
<author><name sortKey="Rosenthal, An" uniqKey="Rosenthal A">AN Rosenthal</name>
</author>
<author><name sortKey="Malysiak, S" uniqKey="Malysiak S">S Malysiak</name>
</author>
<author><name sortKey="Kowalska, M" uniqKey="Kowalska M">M Kowalska</name>
</author>
<author><name sortKey="Zapalska, A" uniqKey="Zapalska A">A Zapalska</name>
</author>
<author><name sortKey="Rogozinska, E" uniqKey="Rogozinska E">E Rogozinska</name>
</author>
<author><name sortKey="Baldwin, P" uniqKey="Baldwin P">P Baldwin</name>
</author>
<author><name sortKey="Ganesan, R" uniqKey="Ganesan R">R Ganesan</name>
</author>
<author><name sortKey="Borowiack, E" uniqKey="Borowiack E">E Borowiack</name>
</author>
<author><name sortKey="Barton, P" uniqKey="Barton P">P Barton</name>
</author>
<author><name sortKey="Roberts, T" uniqKey="Roberts T">T Roberts</name>
</author>
<author><name sortKey="Khan, K" uniqKey="Khan K">K Khan</name>
</author>
<author><name sortKey="Sundar, S" uniqKey="Sundar S">S Sundar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vahrmeijer, Al" uniqKey="Vahrmeijer A">AL Vahrmeijer</name>
</author>
<author><name sortKey="Hutteman, M" uniqKey="Hutteman M">M Hutteman</name>
</author>
<author><name sortKey="Van Der Vorst, Jr" uniqKey="Van Der Vorst J">JR van der Vorst</name>
</author>
<author><name sortKey="Van De Velde, Cjh" uniqKey="Van De Velde C">CJH van de Velde</name>
</author>
<author><name sortKey="Frangioni, Jv" uniqKey="Frangioni J">JV Frangioni</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Holloway, Rw" uniqKey="Holloway R">RW Holloway</name>
</author>
<author><name sortKey="Bravo, Ra" uniqKey="Bravo R">RA Bravo</name>
</author>
<author><name sortKey="Rakowski, Ja" uniqKey="Rakowski J">JA Rakowski</name>
</author>
<author><name sortKey="James, Ja" uniqKey="James J">JA James</name>
</author>
<author><name sortKey="Jeppson, Cn" uniqKey="Jeppson C">CN Jeppson</name>
</author>
<author><name sortKey="Ingersoll, Sb" uniqKey="Ingersoll S">SB Ingersoll</name>
</author>
<author><name sortKey="Ahmad, S" uniqKey="Ahmad S">S Ahmad</name>
</author>
</analytic>
</biblStruct>
<biblStruct></biblStruct>
<biblStruct><analytic><author><name sortKey="Van Der Vorst, Jr" uniqKey="Van Der Vorst J">JR van der Vorst</name>
</author>
<author><name sortKey="Hutteman, M" uniqKey="Hutteman M">M Hutteman</name>
</author>
<author><name sortKey="Gaarenstroom, Kn" uniqKey="Gaarenstroom K">KN Gaarenstroom</name>
</author>
<author><name sortKey="Peters, Aaw" uniqKey="Peters A">AAW Peters</name>
</author>
<author><name sortKey="Mieog, Jsd" uniqKey="Mieog J">JSD Mieog</name>
</author>
<author><name sortKey="Schaafsma, Be" uniqKey="Schaafsma B">BE Schaafsma</name>
</author>
<author><name sortKey="Kuppen, Pjk" uniqKey="Kuppen P">PJK Kuppen</name>
</author>
<author><name sortKey="Frangioni, Jv" uniqKey="Frangioni J">JV Frangioni</name>
</author>
<author><name sortKey="Van De Velde, Cjh" uniqKey="Van De Velde C">CJH van de Velde</name>
</author>
<author><name sortKey="Vahrmeijer, Al" uniqKey="Vahrmeijer A">AL Vahrmeijer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crane, Lma" uniqKey="Crane L">LMA Crane</name>
</author>
<author><name sortKey="Themelis, G" uniqKey="Themelis G">G Themelis</name>
</author>
<author><name sortKey="Arts, Hjg" uniqKey="Arts H">HJG Arts</name>
</author>
<author><name sortKey="Buddingh, Kt" uniqKey="Buddingh K">KT Buddingh</name>
</author>
<author><name sortKey="Brouwers, Ah" uniqKey="Brouwers A">AH Brouwers</name>
</author>
<author><name sortKey="Ntziachristos, V" uniqKey="Ntziachristos V">V Ntziachristos</name>
</author>
<author><name sortKey="Van Dam, Gm" uniqKey="Van Dam G">GM van Dam</name>
</author>
<author><name sortKey="Van Der Zee, Agj" uniqKey="Van Der Zee A">AGJ van der Zee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rossi, Ec" uniqKey="Rossi E">EC Rossi</name>
</author>
<author><name sortKey="Jackson, A" uniqKey="Jackson A">A Jackson</name>
</author>
<author><name sortKey="Ivanova, A" uniqKey="Ivanova A">A Ivanova</name>
</author>
<author><name sortKey="Boggess, Jf" uniqKey="Boggess J">JF Boggess</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schaafsma, Be" uniqKey="Schaafsma B">BE Schaafsma</name>
</author>
<author><name sortKey="Verbeek, Fpr" uniqKey="Verbeek F">FPR Verbeek</name>
</author>
<author><name sortKey="Peters, Aaw" uniqKey="Peters A">AAW Peters</name>
</author>
<author><name sortKey="Van Der Vorst, Jr" uniqKey="Van Der Vorst J">JR van der Vorst</name>
</author>
<author><name sortKey="De Kroon, Cd" uniqKey="De Kroon C">CD de Kroon</name>
</author>
<author><name sortKey="Van Poelgeest, Mie" uniqKey="Van Poelgeest M">MIE van Poelgeest</name>
</author>
<author><name sortKey="Trimbos, Jbmz" uniqKey="Trimbos J">JBMZ Trimbos</name>
</author>
<author><name sortKey="Van De Velde, Cjh" uniqKey="Van De Velde C">CJH van de Velde</name>
</author>
<author><name sortKey="Frangioni, Jv" uniqKey="Frangioni J">JV Frangioni</name>
</author>
<author><name sortKey="Vahrmeijer, Al" uniqKey="Vahrmeijer A">AL Vahrmeijer</name>
</author>
<author><name sortKey="Gaarenstroom, Kn" uniqKey="Gaarenstroom K">KN Gaarenstroom</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frumovitz, M" uniqKey="Frumovitz M">M Frumovitz</name>
</author>
<author><name sortKey="Coleman, Rc" uniqKey="Coleman R">RC Coleman</name>
</author>
<author><name sortKey="Soliman, Pt" uniqKey="Soliman P">PT Soliman</name>
</author>
<author><name sortKey="Ramirez, Pt" uniqKey="Ramirez P">PT Ramirez</name>
</author>
<author><name sortKey="Levenback, Cf" uniqKey="Levenback C">CF Levenback</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Volpi, D" uniqKey="Volpi D">D Volpi</name>
</author>
<author><name sortKey="Tullis, Idc" uniqKey="Tullis I">IDC Tullis</name>
</author>
<author><name sortKey="Laios, A" uniqKey="Laios A">A Laios</name>
</author>
<author><name sortKey="Pathiraja, Pnj" uniqKey="Pathiraja P">PNJ Pathiraja</name>
</author>
<author><name sortKey="Haldar, K" uniqKey="Haldar K">K Haldar</name>
</author>
<author><name sortKey="Ahmed, Aa" uniqKey="Ahmed A">AA Ahmed</name>
</author>
<author><name sortKey="Vojnovic, B" uniqKey="Vojnovic B">B Vojnovic</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Altgassen, C" uniqKey="Altgassen C">C Altgassen</name>
</author>
<author><name sortKey="Hertel, H" uniqKey="Hertel H">H Hertel</name>
</author>
<author><name sortKey="Brandstadt, A" uniqKey="Brandstadt A">A Brandstadt</name>
</author>
<author><name sortKey="Kohler, C" uniqKey="Kohler C">C Kohler</name>
</author>
<author><name sortKey="Durst, M" uniqKey="Durst M">M Durst</name>
</author>
<author><name sortKey="Schneider, A" uniqKey="Schneider A">A Schneider</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kang, S" uniqKey="Kang S">S Kang</name>
</author>
<author><name sortKey="Yoo, Hj" uniqKey="Yoo H">HJ Yoo</name>
</author>
<author><name sortKey="Hwang, Jh" uniqKey="Hwang J">JH Hwang</name>
</author>
<author><name sortKey="Lim, Mc" uniqKey="Lim M">MC Lim</name>
</author>
<author><name sortKey="Seo, Ss" uniqKey="Seo S">SS Seo</name>
</author>
<author><name sortKey="Park, Sy" uniqKey="Park S">SY Park</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Matheron, Hm" uniqKey="Matheron H">HM Matheron</name>
</author>
<author><name sortKey="Van Den Berg, Ns" uniqKey="Van Den Berg N">NS van den Berg</name>
</author>
<author><name sortKey="Brouwer, Or" uniqKey="Brouwer O">OR Brouwer</name>
</author>
<author><name sortKey="Kleinjan, Gh" uniqKey="Kleinjan G">GH Kleinjan</name>
</author>
<author><name sortKey="Van Driel, Wj" uniqKey="Van Driel W">WJ van Driel</name>
</author>
<author><name sortKey="Trum, Jw" uniqKey="Trum J">JW Trum</name>
</author>
<author><name sortKey="Vegt, E" uniqKey="Vegt E">E Vegt</name>
</author>
<author><name sortKey="Kenter, G" uniqKey="Kenter G">G Kenter</name>
</author>
<author><name sortKey="Van Leeuwen, Fw" uniqKey="Van Leeuwen F">FW van Leeuwen</name>
</author>
<author><name sortKey="Valdes Olmos, Ra" uniqKey="Valdes Olmos R">RA Valdes Olmos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Niikura, H" uniqKey="Niikura H">H Niikura</name>
</author>
<author><name sortKey="Kaiho Sakuma, M" uniqKey="Kaiho Sakuma M">M Kaiho-Sakuma</name>
</author>
<author><name sortKey="Tokunaga, H" uniqKey="Tokunaga H">H Tokunaga</name>
</author>
<author><name sortKey="Toyoshima, M" uniqKey="Toyoshima M">M Toyoshima</name>
</author>
<author><name sortKey="Utsunomiya, H" uniqKey="Utsunomiya H">H Utsunomiya</name>
</author>
<author><name sortKey="Nagase, S" uniqKey="Nagase S">S Nagase</name>
</author>
<author><name sortKey="Takano, T" uniqKey="Takano T">T Takano</name>
</author>
<author><name sortKey="Watanabe, M" uniqKey="Watanabe M">M Watanabe</name>
</author>
<author><name sortKey="Ito, K" uniqKey="Ito K">K Ito</name>
</author>
<author><name sortKey="Yaegashi, N" uniqKey="Yaegashi N">N Yaegashi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Abu Rustum, Nr" uniqKey="Abu Rustum N">NR Abu-Rustum</name>
</author>
<author><name sortKey="Khoury Collado, F" uniqKey="Khoury Collado F">F Khoury-Collado</name>
</author>
<author><name sortKey="Pandit Taskar, N" uniqKey="Pandit Taskar N">N Pandit-Taskar</name>
</author>
<author><name sortKey="Soslow, Ra" uniqKey="Soslow R">RA Soslow</name>
</author>
<author><name sortKey="Dao, F" uniqKey="Dao F">F Dao</name>
</author>
<author><name sortKey="Sonoda, Y" uniqKey="Sonoda Y">Y Sonoda</name>
</author>
<author><name sortKey="Levine, Da" uniqKey="Levine D">DA Levine</name>
</author>
<author><name sortKey="Brown, Cl" uniqKey="Brown C">CL Brown</name>
</author>
<author><name sortKey="Chi, Ds" uniqKey="Chi D">DS Chi</name>
</author>
<author><name sortKey="Barakat, Rr" uniqKey="Barakat R">RR Barakat</name>
</author>
<author><name sortKey="Gemignani, Ml" uniqKey="Gemignani M">ML Gemignani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jewell, El" uniqKey="Jewell E">EL Jewell</name>
</author>
<author><name sortKey="Huang, Jj" uniqKey="Huang J">JJ Huang</name>
</author>
<author><name sortKey="Abu Rustum, Nr" uniqKey="Abu Rustum N">NR Abu-Rustum</name>
</author>
<author><name sortKey="Gardner, Gj" uniqKey="Gardner G">GJ Gardner</name>
</author>
<author><name sortKey="Brown, Cl" uniqKey="Brown C">CL Brown</name>
</author>
<author><name sortKey="Sonoda, Y" uniqKey="Sonoda Y">Y Sonoda</name>
</author>
<author><name sortKey="Barakat, Rr" uniqKey="Barakat R">RR Barakat</name>
</author>
<author><name sortKey="Levine, Da" uniqKey="Levine D">DA Levine</name>
</author>
<author><name sortKey="Leitao, Mm" uniqKey="Leitao M">MM Leitao</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sadeghi, R" uniqKey="Sadeghi R">R Sadeghi</name>
</author>
<author><name sortKey="Hasanzadeh, M" uniqKey="Hasanzadeh M">M Hasanzadeh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xiong, L" uniqKey="Xiong L">L Xiong</name>
</author>
<author><name sortKey="Gazyakan, E" uniqKey="Gazyakan E">E Gazyakan</name>
</author>
<author><name sortKey="Yang, W" uniqKey="Yang W">W Yang</name>
</author>
<author><name sortKey="Engel, H" uniqKey="Engel H">H Engel</name>
</author>
<author><name sortKey="Hunerbein, M" uniqKey="Hunerbein M">M Hunerbein</name>
</author>
<author><name sortKey="Kneser, U" uniqKey="Kneser U">U Kneser</name>
</author>
<author><name sortKey="Hirche, C" uniqKey="Hirche C">C Hirche</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="How, J" uniqKey="How J">J How</name>
</author>
<author><name sortKey="Lau, S" uniqKey="Lau S">S Lau</name>
</author>
<author><name sortKey="Press, J" uniqKey="Press J">J Press</name>
</author>
<author><name sortKey="Ferenczy, A" uniqKey="Ferenczy A">A Ferenczy</name>
</author>
<author><name sortKey="Pelmus, M" uniqKey="Pelmus M">M Pelmus</name>
</author>
<author><name sortKey="Stern, J" uniqKey="Stern J">J Stern</name>
</author>
<author><name sortKey="Probst, S" uniqKey="Probst S">S Probst</name>
</author>
<author><name sortKey="Brin, S" uniqKey="Brin S">S Brin</name>
</author>
<author><name sortKey="Drummond, N" uniqKey="Drummond N">N Drummond</name>
</author>
<author><name sortKey="Gotlieb, W" uniqKey="Gotlieb W">W Gotlieb</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Perrone, Am" uniqKey="Perrone A">AM Perrone</name>
</author>
<author><name sortKey="Casadio, P" uniqKey="Casadio P">P Casadio</name>
</author>
<author><name sortKey="Formelli, G" uniqKey="Formelli G">G Formelli</name>
</author>
<author><name sortKey="Levorato, M" uniqKey="Levorato M">M Levorato</name>
</author>
<author><name sortKey="Ghi, T" uniqKey="Ghi T">T Ghi</name>
</author>
<author><name sortKey="Costa, S" uniqKey="Costa S">S Costa</name>
</author>
<author><name sortKey="Meriggiola, Mc" uniqKey="Meriggiola M">MC Meriggiola</name>
</author>
<author><name sortKey="Pelusi, G" uniqKey="Pelusi G">G Pelusi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Furukawa, N" uniqKey="Furukawa N">N Furukawa</name>
</author>
<author><name sortKey="Oi, H" uniqKey="Oi H">H Oi</name>
</author>
<author><name sortKey="Yoshida, S" uniqKey="Yoshida S">S Yoshida</name>
</author>
<author><name sortKey="Shigetomi, H" uniqKey="Shigetomi H">H Shigetomi</name>
</author>
<author><name sortKey="Kanayama, S" uniqKey="Kanayama S">S Kanayama</name>
</author>
<author><name sortKey="Kobayashi, H" uniqKey="Kobayashi H">H Kobayashi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crane, Lm" uniqKey="Crane L">LM Crane</name>
</author>
<author><name sortKey="Themelis, G" uniqKey="Themelis G">G Themelis</name>
</author>
<author><name sortKey="Pleijhuis, Rg" uniqKey="Pleijhuis R">RG Pleijhuis</name>
</author>
<author><name sortKey="Harlaar, Nj" uniqKey="Harlaar N">NJ Harlaar</name>
</author>
<author><name sortKey="Sarantopoulos, A" uniqKey="Sarantopoulos A">A Sarantopoulos</name>
</author>
<author><name sortKey="Arts, Hj" uniqKey="Arts H">HJ Arts</name>
</author>
<author><name sortKey="Van Der Zee, Ag" uniqKey="Van Der Zee A">AG van der Zee</name>
</author>
<author><name sortKey="Ntziachristos, V" uniqKey="Ntziachristos V">V Ntziachristos</name>
</author>
<author><name sortKey="Van Dam, Gm" uniqKey="Van Dam G">GM van Dam</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rossi, Ec" uniqKey="Rossi E">EC Rossi</name>
</author>
<author><name sortKey="Ivanova, A" uniqKey="Ivanova A">A Ivanova</name>
</author>
<author><name sortKey="Boggess, Jf" uniqKey="Boggess J">JF Boggess</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schaafsma, Be" uniqKey="Schaafsma B">BE Schaafsma</name>
</author>
<author><name sortKey="Van Der Vorst, Jr" uniqKey="Van Der Vorst J">JR van der Vorst</name>
</author>
<author><name sortKey="Gaarenstroom, Kn" uniqKey="Gaarenstroom K">KN Gaarenstroom</name>
</author>
<author><name sortKey="Peters, Aa" uniqKey="Peters A">AA Peters</name>
</author>
<author><name sortKey="Verbeek, Fp" uniqKey="Verbeek F">FP Verbeek</name>
</author>
<author><name sortKey="De Kroon, Cd" uniqKey="De Kroon C">CD de Kroon</name>
</author>
<author><name sortKey="Trimbos, Jb" uniqKey="Trimbos J">JB Trimbos</name>
</author>
<author><name sortKey="Van Poelgeest, Mi" uniqKey="Van Poelgeest M">MI van Poelgeest</name>
</author>
<author><name sortKey="Frangioni, Jv" uniqKey="Frangioni J">JV Frangioni</name>
</author>
<author><name sortKey="Van De Velde, Cj" uniqKey="Van De Velde C">CJ van de Velde</name>
</author>
<author><name sortKey="Vahrmeijer, Al" uniqKey="Vahrmeijer A">AL Vahrmeijer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sinno, Ak" uniqKey="Sinno A">AK Sinno</name>
</author>
<author><name sortKey="Fader, An" uniqKey="Fader A">AN Fader</name>
</author>
<author><name sortKey="Roche, Kl" uniqKey="Roche K">KL Roche</name>
</author>
<author><name sortKey="Giuntoli, Rl" uniqKey="Giuntoli R">RL Giuntoli</name>
</author>
<author><name sortKey="Tanner, Ej" uniqKey="Tanner E">EJ Tanner</name>
</author>
</analytic>
</biblStruct>
</listBibl>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">BMC Res Notes</journal-id>
<journal-id journal-id-type="iso-abbrev">BMC Res Notes</journal-id>
<journal-title-group><journal-title>BMC Research Notes</journal-title>
</journal-title-group>
<issn pub-type="epub">1756-0500</issn>
<publisher><publisher-name>BioMed Central</publisher-name>
<publisher-loc>London</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">26502876</article-id>
<article-id pub-id-type="pmc">4621870</article-id>
<article-id pub-id-type="publisher-id">1576</article-id>
<article-id pub-id-type="doi">10.1186/s13104-015-1576-z</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>A prospective pilot study of detection of sentinel lymph nodes in gynaecological cancers using a novel near infrared fluorescence imaging system</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" equal-contrib="yes"><name><surname>Laios</surname>
<given-names>Alexandros</given-names>
</name>
<address><email>a.laios@nhs.net</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author" equal-contrib="yes"><name><surname>Volpi</surname>
<given-names>Davide</given-names>
</name>
<address><email>davide.volpi@oncology.ox.ac.uk</email>
</address>
<xref ref-type="aff" rid="Aff3"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tullis</surname>
<given-names>Iain D. C.</given-names>
</name>
<address><email>iain.tullis@oncology.ox.ac.uk</email>
</address>
<xref ref-type="aff" rid="Aff3"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Woodward</surname>
<given-names>Martha</given-names>
</name>
<address><email>martha.woodward@oncology.ox.ac.uk</email>
</address>
<xref ref-type="aff" rid="Aff4"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kennedy</surname>
<given-names>Stephen</given-names>
</name>
<address><email>stephen.kennedy@obs-gyn.ox.ac.uk</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pathiraja</surname>
<given-names>Pubudu N. J.</given-names>
</name>
<address><email>pubudu.pathiraja@obs-gyn.ox.ac.uk</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Haldar</surname>
<given-names>Krishnayan</given-names>
</name>
<address><email>Krishnayan.Haldar@ouh.nhs.uk</email>
</address>
<xref ref-type="aff" rid="Aff2"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Vojnovic</surname>
<given-names>Borivoj</given-names>
</name>
<address><email>boris.vojnovic@oncology.ox.ac.uk</email>
</address>
<xref ref-type="aff" rid="Aff3"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Ahmed</surname>
<given-names>Ahmed A.</given-names>
</name>
<address><phone>+44(0) 1865 222820</phone>
<email>ahmed.ahmed@obs-gyn.ox.ac.uk</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
<xref ref-type="aff" rid="Aff2"></xref>
<xref ref-type="aff" rid="Aff5"></xref>
</contrib>
<aff id="Aff1"><label></label>
Nuffield Department of Obstetrics and Gynaecology, University of Oxford, Oxford, UK</aff>
<aff id="Aff2"><label></label>
Gynaecological Oncology Unit, Churchill Hospital, Oxford University Hospitals NHS Trust, Oxford, UK</aff>
<aff id="Aff3"><label></label>
Department of Oncology, CRUK/MRC Oxford Institute for Radiation Oncology, University of Oxford, Oxford, UK</aff>
<aff id="Aff4"><label></label>
Early Phase Research Hub, Department of Oncology, Oxford Cancer and Haematology Centre, Oxford University Hospitals NHS Trust, Oxford, UK</aff>
<aff id="Aff5"><label></label>
Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, Headington, Oxford, OX37DS UK</aff>
</contrib-group>
<pub-date pub-type="epub"><day>26</day>
<month>10</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>26</day>
<month>10</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="collection"><year>2015</year>
</pub-date>
<volume>8</volume>
<elocation-id>608</elocation-id>
<history><date date-type="received"><day>28</day>
<month>5</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>13</day>
<month>10</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>© Laios et al. 2015</copyright-statement>
<license license-type="OpenAccess"><license-p><bold>Open Access</bold>
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>
), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/publicdomain/zero/1.0/">http://creativecommons.org/publicdomain/zero/1.0/</ext-link>
) applies to the data made available in this article, unless otherwise stated.</license-p>
</license>
</permissions>
<abstract id="Abs1"><sec><title>Background</title>
<p>Sentinel Lymph Node (SLN) sampling may significantly reduce surgical morbidity by avoiding needless radical lymphadenectomy. In gynaecological cancers, the current practice in the UK is testing the accuracy of SLN detection using radioactive isotopes within the context of clinical trials. However, radioactive tracers pose significant logistic problems. We, therefore, conducted a pilot, observational study to assess the feasibility of a novel optical imaging device for SLN detection in gynaecological cancers using near infrared (NIR) fluorescence.</p>
</sec>
<sec><title>Methods</title>
<p>A novel, custom-made, optical imaging system was developed to enable detection of multiple fluorescence dyes and allow simultaneous bright-field imaging during open surgery and laparoscopic procedures. We then evaluated the performance of the system in a prospective study of 49 women with early stage vulval, cervical and endometrial cancer who were scheduled to undergo complete lymphadenectomy. Clinically approved fluorescent contrast agents indocyanine green (ICG) and methylene blue (MB) were used. The main outcomes of the study included SLN mapping detection rates, false negative rates using the NIR fluorescence technique and safety of the procedures. We also examined the association between injection sites and differential lymphatic drainage in women with endometrial cancer by fluorescence imaging of ICG and MB.</p>
</sec>
<sec><title>Results</title>
<p>A total of 64 SLNs were detected during both open surgery and laparoscopy. Following dose optimisation and the learning phase, SLN detection rate approached 100 % for all cancer types with no false negatives detected. Fluorescence from ICG and MB detected para-aortic SLNs in women with endometrial cancer following uterine injection. Percutaneous SLN detection was also achieved in most women with vulval cancer. No adverse reactions associated with the use of either dyes were observed.</p>
</sec>
<sec><title>Conclusions</title>
<p>This study demonstrated the successful clinical application of a novel NIR fluorescence imaging system for SLN detection across different gynaecological cancers. We showcased the first <italic>in human</italic>
imaging, during the same procedure, of two fluorescence dyes in women with endometrial cancer.</p>
</sec>
<sec><title>Electronic supplementary material</title>
<p>The online version of this article (doi:10.1186/s13104-015-1576-z) contains supplementary material, which is available to authorized users.</p>
</sec>
</abstract>
<kwd-group xml:lang="en"><title>Keywords</title>
<kwd>Gynaecological cancer</kwd>
<kwd>Sentinel lymph node</kwd>
<kwd>Near infrared fluorescence</kwd>
<kwd>Optical imaging</kwd>
<kwd>Indocyanine green</kwd>
<kwd>Methylene blue</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© The Author(s) 2015</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Royaume-Uni</li>
</country>
<region><li>Angleterre</li>
<li>Oxfordshire</li>
</region>
<settlement><li>Oxford</li>
</settlement>
<orgName><li>Université d'Oxford</li>
</orgName>
</list>
<tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Laios, Alexandros" sort="Laios, Alexandros" uniqKey="Laios A" first="Alexandros" last="Laios">Alexandros Laios</name>
</region>
<name sortKey="Ahmed, Ahmed A" sort="Ahmed, Ahmed A" uniqKey="Ahmed A" first="Ahmed A." last="Ahmed">Ahmed A. Ahmed</name>
<name sortKey="Ahmed, Ahmed A" sort="Ahmed, Ahmed A" uniqKey="Ahmed A" first="Ahmed A." last="Ahmed">Ahmed A. Ahmed</name>
<name sortKey="Ahmed, Ahmed A" sort="Ahmed, Ahmed A" uniqKey="Ahmed A" first="Ahmed A." last="Ahmed">Ahmed A. Ahmed</name>
<name sortKey="Haldar, Krishnayan" sort="Haldar, Krishnayan" uniqKey="Haldar K" first="Krishnayan" last="Haldar">Krishnayan Haldar</name>
<name sortKey="Kennedy, Stephen" sort="Kennedy, Stephen" uniqKey="Kennedy S" first="Stephen" last="Kennedy">Stephen Kennedy</name>
<name sortKey="Laios, Alexandros" sort="Laios, Alexandros" uniqKey="Laios A" first="Alexandros" last="Laios">Alexandros Laios</name>
<name sortKey="Pathiraja, Pubudu N J" sort="Pathiraja, Pubudu N J" uniqKey="Pathiraja P" first="Pubudu N. J." last="Pathiraja">Pubudu N. J. Pathiraja</name>
<name sortKey="Pathiraja, Pubudu N J" sort="Pathiraja, Pubudu N J" uniqKey="Pathiraja P" first="Pubudu N. J." last="Pathiraja">Pubudu N. J. Pathiraja</name>
<name sortKey="Tullis, Iain D C" sort="Tullis, Iain D C" uniqKey="Tullis I" first="Iain D. C." last="Tullis">Iain D. C. Tullis</name>
<name sortKey="Vojnovic, Borivoj" sort="Vojnovic, Borivoj" uniqKey="Vojnovic B" first="Borivoj" last="Vojnovic">Borivoj Vojnovic</name>
<name sortKey="Volpi, Davide" sort="Volpi, Davide" uniqKey="Volpi D" first="Davide" last="Volpi">Davide Volpi</name>
<name sortKey="Woodward, Martha" sort="Woodward, Martha" uniqKey="Woodward M" first="Martha" last="Woodward">Martha Woodward</name>
</country>
</tree>
</affiliations>
</record>
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