Postnatal Chick Choroids Exhibit Increased Retinaldehyde Dehydrogenase Activity During Recovery From Form Deprivation Induced Myopia
Identifieur interne : 000693 ( Pmc/Checkpoint ); précédent : 000692; suivant : 000694Postnatal Chick Choroids Exhibit Increased Retinaldehyde Dehydrogenase Activity During Recovery From Form Deprivation Induced Myopia
Auteurs : Angelica R. Harper [États-Unis] ; Xiang Wang [États-Unis] ; Gennadiy Moiseyev [États-Unis] ; Jian-Xing Ma [États-Unis] ; Jody A. Summers [États-Unis]Source :
- Investigative Ophthalmology & Visual Science [ 0146-0404 ] ; 2016.
Abstract
Increases in retinaldehyde dehydrogenase 2 (
Myopia was induced in White Leghorn chicks for 10 days, followed by up to 15 days of unrestricted vision (recovery). Expression of RALDH isoforms in chick ocular tissues was evaluated by Western blot. Catalytic activity of RALDH was measured in choroidal cytosol fractions using an in vitro atRA synthesis assay together with HPLC quantification of synthesized atRA. Distribution of RALDH2 cells throughout the choroid was evaluated by immunohistochemistry.
RALDH2 was expressed predominately in the chick choroid (
The results of this study suggest that RALDH2 is the major RALDH isoform in the chick choroid and is responsible for the increased RALDH activity seen during recovery.
Url:
DOI: 10.1167/iovs.16-19395
PubMed: 27654415
PubMed Central: 5032914
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Postnatal Chick Choroids Exhibit Increased Retinaldehyde Dehydrogenase Activity During Recovery From Form Deprivation Induced Myopia</title><author><name sortKey="Harper, Angelica R" sort="Harper, Angelica R" uniqKey="Harper A" first="Angelica R." last="Harper">Angelica R. Harper</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma</wicri:regionArea><placeName><region type="state">Oklahoma</region></placeName></affiliation></author><author><name sortKey="Wang, Xiang" sort="Wang, Xiang" uniqKey="Wang X" first="Xiang" last="Wang">Xiang Wang</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma</wicri:regionArea><placeName><region type="state">Oklahoma</region></placeName></affiliation></author><author><name sortKey="Moiseyev, Gennadiy" sort="Moiseyev, Gennadiy" uniqKey="Moiseyev G" first="Gennadiy" last="Moiseyev">Gennadiy Moiseyev</name><affiliation wicri:level="2"><nlm:aff id="aff2">Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma</wicri:regionArea><placeName><region type="state">Oklahoma</region></placeName></affiliation></author><author><name sortKey="Ma, Jian Xing" sort="Ma, Jian Xing" uniqKey="Ma J" first="Jian-Xing" last="Ma">Jian-Xing Ma</name><affiliation wicri:level="2"><nlm:aff id="aff2">Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma</wicri:regionArea><placeName><region type="state">Oklahoma</region></placeName></affiliation></author><author><name sortKey="Summers, Jody A" sort="Summers, Jody A" uniqKey="Summers J" first="Jody A." last="Summers">Jody A. Summers</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma</wicri:regionArea><placeName><region type="state">Oklahoma</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27654415</idno><idno type="pmc">5032914</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5032914</idno><idno type="RBID">PMC:5032914</idno><idno type="doi">10.1167/iovs.16-19395</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">004005</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">004005</idno><idno type="wicri:Area/Pmc/Curation">004004</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">004004</idno><idno type="wicri:Area/Pmc/Checkpoint">000693</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000693</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Postnatal Chick Choroids Exhibit Increased Retinaldehyde Dehydrogenase Activity During Recovery From Form Deprivation Induced Myopia</title><author><name sortKey="Harper, Angelica R" sort="Harper, Angelica R" uniqKey="Harper A" first="Angelica R." last="Harper">Angelica R. Harper</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma</wicri:regionArea><placeName><region type="state">Oklahoma</region></placeName></affiliation></author><author><name sortKey="Wang, Xiang" sort="Wang, Xiang" uniqKey="Wang X" first="Xiang" last="Wang">Xiang Wang</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma</wicri:regionArea><placeName><region type="state">Oklahoma</region></placeName></affiliation></author><author><name sortKey="Moiseyev, Gennadiy" sort="Moiseyev, Gennadiy" uniqKey="Moiseyev G" first="Gennadiy" last="Moiseyev">Gennadiy Moiseyev</name><affiliation wicri:level="2"><nlm:aff id="aff2">Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma</wicri:regionArea><placeName><region type="state">Oklahoma</region></placeName></affiliation></author><author><name sortKey="Ma, Jian Xing" sort="Ma, Jian Xing" uniqKey="Ma J" first="Jian-Xing" last="Ma">Jian-Xing Ma</name><affiliation wicri:level="2"><nlm:aff id="aff2">Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma</wicri:regionArea><placeName><region type="state">Oklahoma</region></placeName></affiliation></author><author><name sortKey="Summers, Jody A" sort="Summers, Jody A" uniqKey="Summers J" first="Jody A." last="Summers">Jody A. Summers</name><affiliation wicri:level="2"><nlm:aff id="aff1">Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma</wicri:regionArea><placeName><region type="state">Oklahoma</region></placeName></affiliation></author></analytic><series><title level="j">Investigative Ophthalmology & Visual Science</title><idno type="ISSN">0146-0404</idno><idno type="eISSN">1552-5783</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec id="st1"><title>Purpose</title><p>Increases in retinaldehyde dehydrogenase 2 (<italic>RALDH2</italic>) transcript in the chick choroid suggest that RALDH2 may be responsible for increases observed in all-trans-retinoic acid (atRA) synthesis during recovery from myopic defocus. The purpose of the present study was to examine RALDH2 protein expression, RALDH activity, and distribution of RALDH2 cells in control and recovering chick ocular tissues.</p></sec><sec id="st2"><title>Methods</title><p>Myopia was induced in White Leghorn chicks for 10 days, followed by up to 15 days of unrestricted vision (recovery). Expression of RALDH isoforms in chick ocular tissues was evaluated by Western blot. Catalytic activity of RALDH was measured in choroidal cytosol fractions using an in vitro atRA synthesis assay together with HPLC quantification of synthesized atRA. Distribution of RALDH2 cells throughout the choroid was evaluated by immunohistochemistry.</p></sec><sec id="st3"><title>Results</title><p>RALDH2 was expressed predominately in the chick choroid (<italic>P</italic> < 0.001) and increased after 24 hours and 4 days of recovery (76%, 74%, and 165%, respectively; <italic>P</italic> < 0.05). Activity of RALDH was detected solely in the choroid and was elevated at 3 and 7 days of recovery compared to controls (70% and 48%, respectively; <italic>P</italic> < 0.05). The number of RALDH2 immunopositive cells in recovering choroids was increased at 24 hours and 4 to 15 days of recovery (<italic>P</italic> < 0.05) and were concentrated toward the RPE side compared to controls.</p></sec><sec id="st4"><title>Conclusions</title><p>The results of this study suggest that RALDH2 is the major RALDH isoform in the chick choroid and is responsible for the increased RALDH activity seen during recovery.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="O Leary, Dj" uniqKey="O Leary D">DJ, O'Leary</name></author><author><name sortKey="Millodot, M" uniqKey="Millodot M">M. 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Crewther</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Burger, Nb" uniqKey="Burger N">NB, Burger</name></author><author><name sortKey="Stuurman, Ke" uniqKey="Stuurman K">KE, Stuurman</name></author><author><name sortKey="Kok, E" uniqKey="Kok E">E, Kok</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Choi, I" uniqKey="Choi I">I, Choi</name></author><author><name sortKey="Lee, S" uniqKey="Lee S">S, Lee</name></author><author><name sortKey="Kyoung Chung, H" uniqKey="Kyoung Chung H">H, Kyoung Chung</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Invest Ophthalmol Vis Sci</journal-id><journal-id journal-id-type="iso-abbrev">Invest. Ophthalmol. Vis. Sci</journal-id><journal-id journal-id-type="hwp">iovs</journal-id><journal-id journal-id-type="pmc">iovs</journal-id><journal-id journal-id-type="publisher-id">iovs</journal-id><journal-title-group><journal-title>Investigative Ophthalmology & Visual Science</journal-title></journal-title-group><issn pub-type="ppub">0146-0404</issn><issn pub-type="epub">1552-5783</issn><publisher><publisher-name>The Association for Research in Vision and Ophthalmology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">27654415</article-id><article-id pub-id-type="pmc">5032914</article-id><article-id pub-id-type="doi">10.1167/iovs.16-19395</article-id><article-id pub-id-type="sici">iovs-57-12-12</article-id><article-id pub-id-type="publisher-id">IOVS-16-19395</article-id><article-categories><subj-group subj-group-type="heading"><subject>Anatomy and Pathology/Oncology</subject></subj-group></article-categories><title-group><article-title>Postnatal Chick Choroids Exhibit Increased Retinaldehyde Dehydrogenase Activity During Recovery From Form Deprivation Induced Myopia</article-title><alt-title alt-title-type="runhead">Retinaldehyde Dehydrogenase Activity in Myopia</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Harper</surname><given-names>Angelica R.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Xiang</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Moiseyev</surname><given-names>Gennadiy</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Ma</surname><given-names>Jian-Xing</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Summers</surname><given-names>Jody A.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><aff id="aff1"><label>1</label>Department of Cell Biology University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</aff><aff id="aff2"><label>2</label>Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, United States</aff></contrib-group><author-notes><corresp id="cor1">Correspondence: Angelica R. Harper, Department of Cell Biology, University of Oklahoma Health Science Center, BRC, Room 266, 975 NE 10th Street, Oklahoma City, Oklahoma 73104, USA; <email>angelica-harper@ouhsc.edu</email>.</corresp></author-notes><pub-date pub-type="ppub"><month>9</month><year>2016</year></pub-date><volume>57</volume><issue>11</issue><fpage>4886</fpage><lpage>4897</lpage><history><date date-type="received"><day>19</day><month>2</month><year>2016</year></date><date date-type="accepted"><day>30</day><month>6</month><year>2016</year></date></history><permissions><license license-type="cc-by-nc-nd" xlink:href="http://creativecommons.org/licenses/by-nc-nd/4.0/"><license-p>This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.</license-p></license></permissions><self-uri xlink:title="pdf" xlink:href="i1552-5783-57-11-4886.pdf"></self-uri><abstract><sec id="st1"><title>Purpose</title><p>Increases in retinaldehyde dehydrogenase 2 (<italic>RALDH2</italic>) transcript in the chick choroid suggest that RALDH2 may be responsible for increases observed in all-trans-retinoic acid (atRA) synthesis during recovery from myopic defocus. The purpose of the present study was to examine RALDH2 protein expression, RALDH activity, and distribution of RALDH2 cells in control and recovering chick ocular tissues.</p></sec><sec id="st2"><title>Methods</title><p>Myopia was induced in White Leghorn chicks for 10 days, followed by up to 15 days of unrestricted vision (recovery). Expression of RALDH isoforms in chick ocular tissues was evaluated by Western blot. Catalytic activity of RALDH was measured in choroidal cytosol fractions using an in vitro atRA synthesis assay together with HPLC quantification of synthesized atRA. Distribution of RALDH2 cells throughout the choroid was evaluated by immunohistochemistry.</p></sec><sec id="st3"><title>Results</title><p>RALDH2 was expressed predominately in the chick choroid (<italic>P</italic> < 0.001) and increased after 24 hours and 4 days of recovery (76%, 74%, and 165%, respectively; <italic>P</italic> < 0.05). Activity of RALDH was detected solely in the choroid and was elevated at 3 and 7 days of recovery compared to controls (70% and 48%, respectively; <italic>P</italic> < 0.05). The number of RALDH2 immunopositive cells in recovering choroids was increased at 24 hours and 4 to 15 days of recovery (<italic>P</italic> < 0.05) and were concentrated toward the RPE side compared to controls.</p></sec><sec id="st4"><title>Conclusions</title><p>The results of this study suggest that RALDH2 is the major RALDH isoform in the chick choroid and is responsible for the increased RALDH activity seen during recovery.</p></sec></abstract><kwd-group><title>Keywords</title><kwd>myopia</kwd><kwd>retinoic acid</kwd><kwd>choroid</kwd><kwd>RALDH2</kwd><kwd>form deprivation</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>États-Unis</li></country><region><li>Oklahoma</li></region></list><tree><country name="États-Unis"><region name="Oklahoma"><name sortKey="Harper, Angelica R" sort="Harper, Angelica R" uniqKey="Harper A" first="Angelica R." last="Harper">Angelica R. Harper</name></region><name sortKey="Ma, Jian Xing" sort="Ma, Jian Xing" uniqKey="Ma J" first="Jian-Xing" last="Ma">Jian-Xing Ma</name><name sortKey="Moiseyev, Gennadiy" sort="Moiseyev, Gennadiy" uniqKey="Moiseyev G" first="Gennadiy" last="Moiseyev">Gennadiy Moiseyev</name><name sortKey="Summers, Jody A" sort="Summers, Jody A" uniqKey="Summers J" first="Jody A." last="Summers">Jody A. Summers</name><name sortKey="Wang, Xiang" sort="Wang, Xiang" uniqKey="Wang X" first="Xiang" last="Wang">Xiang Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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