ATRAP Expression in Brown Adipose Tissue Does Not Influence the Development of Diet-Induced Metabolic Disorders in Mice
Identifieur interne : 000421 ( Pmc/Checkpoint ); précédent : 000420; suivant : 000422ATRAP Expression in Brown Adipose Tissue Does Not Influence the Development of Diet-Induced Metabolic Disorders in Mice
Auteurs : Kohji Ohki ; Hiromichi Wakui ; Kengo Azushima [Singapour] ; Kazushi Uneda ; Sona Haku ; Ryu Kobayashi ; Kotaro Haruhara ; Sho Kinguchi ; Miyuki Matsuda ; Masato Ohsawa ; Akinobu Maeda ; Shintaro Minegishi ; Tomoaki Ishigami ; Yoshiyuki Toya ; Akio Yamashita ; Satoshi Umemura [Japon] ; Kouichi TamuraSource :
- International Journal of Molecular Sciences [ 1422-0067 ] ; 2017.
Abstract
Activation of tissue renin–angiotensin system (RAS), mainly mediated by an angiotensin II (Ang II) type 1 receptor (AT1R), plays an important role in the development of obesity-related metabolic disorders. We have shown that AT1R-associated protein (ATRAP), a specific binding protein of AT1R, functions as an endogenous inhibitor to prevent excessive activation of tissue RAS. In the present study, we newly generated ATRAP/
Url:
DOI: 10.3390/ijms18030676
PubMed: 28335584
PubMed Central: 5372686
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">ATRAP Expression in Brown Adipose Tissue Does Not Influence the Development of Diet-Induced Metabolic Disorders in Mice</title><author><name sortKey="Ohki, Kohji" sort="Ohki, Kohji" uniqKey="Ohki K" first="Kohji" last="Ohki">Kohji Ohki</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Wakui, Hiromichi" sort="Wakui, Hiromichi" uniqKey="Wakui H" first="Hiromichi" last="Wakui">Hiromichi Wakui</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Azushima, Kengo" sort="Azushima, Kengo" uniqKey="Azushima K" first="Kengo" last="Azushima">Kengo Azushima</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="af2-ijms-18-00676">Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 8 College Road, Singapore 169857</wicri:regionArea><wicri:noRegion>Singapore 169857</wicri:noRegion></affiliation></author><author><name sortKey="Uneda, Kazushi" sort="Uneda, Kazushi" uniqKey="Uneda K" first="Kazushi" last="Uneda">Kazushi Uneda</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Haku, Sona" sort="Haku, Sona" uniqKey="Haku S" first="Sona" last="Haku">Sona Haku</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Kobayashi, Ryu" sort="Kobayashi, Ryu" uniqKey="Kobayashi R" first="Ryu" last="Kobayashi">Ryu Kobayashi</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Haruhara, Kotaro" sort="Haruhara, Kotaro" uniqKey="Haruhara K" first="Kotaro" last="Haruhara">Kotaro Haruhara</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Kinguchi, Sho" sort="Kinguchi, Sho" uniqKey="Kinguchi S" first="Sho" last="Kinguchi">Sho Kinguchi</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Matsuda, Miyuki" sort="Matsuda, Miyuki" uniqKey="Matsuda M" first="Miyuki" last="Matsuda">Miyuki Matsuda</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Ohsawa, Masato" sort="Ohsawa, Masato" uniqKey="Ohsawa M" first="Masato" last="Ohsawa">Masato Ohsawa</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Maeda, Akinobu" sort="Maeda, Akinobu" uniqKey="Maeda A" first="Akinobu" last="Maeda">Akinobu Maeda</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Minegishi, Shintaro" sort="Minegishi, Shintaro" uniqKey="Minegishi S" first="Shintaro" last="Minegishi">Shintaro Minegishi</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Ishigami, Tomoaki" sort="Ishigami, Tomoaki" uniqKey="Ishigami T" first="Tomoaki" last="Ishigami">Tomoaki Ishigami</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Toya, Yoshiyuki" sort="Toya, Yoshiyuki" uniqKey="Toya Y" first="Yoshiyuki" last="Toya">Yoshiyuki Toya</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Yamashita, Akio" sort="Yamashita, Akio" uniqKey="Yamashita A" first="Akio" last="Yamashita">Akio Yamashita</name><affiliation><nlm:aff id="af3-ijms-18-00676">Department of Molecular Biology, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>yamasita@yokohama-cu.ac.jp</email></nlm:aff></affiliation></author><author><name sortKey="Umemura, Satoshi" sort="Umemura, Satoshi" uniqKey="Umemura S" first="Satoshi" last="Umemura">Satoshi Umemura</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="af4-ijms-18-00676">Yokohama Rosai Hospital, 3211 Kozukue-cho, Kohoku-ku, Yokohama 222-0036, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Yokohama Rosai Hospital, 3211 Kozukue-cho, Kohoku-ku, Yokohama 222-0036</wicri:regionArea><wicri:noRegion>Yokohama 222-0036</wicri:noRegion></affiliation></author><author><name sortKey="Tamura, Kouichi" sort="Tamura, Kouichi" uniqKey="Tamura K" first="Kouichi" last="Tamura">Kouichi Tamura</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">28335584</idno><idno type="pmc">5372686</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5372686</idno><idno type="RBID">PMC:5372686</idno><idno type="doi">10.3390/ijms18030676</idno><date when="2017">2017</date><idno type="wicri:Area/Pmc/Corpus">003F71</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003F71</idno><idno type="wicri:Area/Pmc/Curation">003F70</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003F70</idno><idno type="wicri:Area/Pmc/Checkpoint">000421</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000421</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">ATRAP Expression in Brown Adipose Tissue Does Not Influence the Development of Diet-Induced Metabolic Disorders in Mice</title><author><name sortKey="Ohki, Kohji" sort="Ohki, Kohji" uniqKey="Ohki K" first="Kohji" last="Ohki">Kohji Ohki</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Wakui, Hiromichi" sort="Wakui, Hiromichi" uniqKey="Wakui H" first="Hiromichi" last="Wakui">Hiromichi Wakui</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Azushima, Kengo" sort="Azushima, Kengo" uniqKey="Azushima K" first="Kengo" last="Azushima">Kengo Azushima</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="af2-ijms-18-00676">Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore</nlm:aff><country xml:lang="fr">Singapour</country><wicri:regionArea>Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 8 College Road, Singapore 169857</wicri:regionArea><wicri:noRegion>Singapore 169857</wicri:noRegion></affiliation></author><author><name sortKey="Uneda, Kazushi" sort="Uneda, Kazushi" uniqKey="Uneda K" first="Kazushi" last="Uneda">Kazushi Uneda</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Haku, Sona" sort="Haku, Sona" uniqKey="Haku S" first="Sona" last="Haku">Sona Haku</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Kobayashi, Ryu" sort="Kobayashi, Ryu" uniqKey="Kobayashi R" first="Ryu" last="Kobayashi">Ryu Kobayashi</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Haruhara, Kotaro" sort="Haruhara, Kotaro" uniqKey="Haruhara K" first="Kotaro" last="Haruhara">Kotaro Haruhara</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Kinguchi, Sho" sort="Kinguchi, Sho" uniqKey="Kinguchi S" first="Sho" last="Kinguchi">Sho Kinguchi</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Matsuda, Miyuki" sort="Matsuda, Miyuki" uniqKey="Matsuda M" first="Miyuki" last="Matsuda">Miyuki Matsuda</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Ohsawa, Masato" sort="Ohsawa, Masato" uniqKey="Ohsawa M" first="Masato" last="Ohsawa">Masato Ohsawa</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Maeda, Akinobu" sort="Maeda, Akinobu" uniqKey="Maeda A" first="Akinobu" last="Maeda">Akinobu Maeda</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Minegishi, Shintaro" sort="Minegishi, Shintaro" uniqKey="Minegishi S" first="Shintaro" last="Minegishi">Shintaro Minegishi</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Ishigami, Tomoaki" sort="Ishigami, Tomoaki" uniqKey="Ishigami T" first="Tomoaki" last="Ishigami">Tomoaki Ishigami</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Toya, Yoshiyuki" sort="Toya, Yoshiyuki" uniqKey="Toya Y" first="Yoshiyuki" last="Toya">Yoshiyuki Toya</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author><author><name sortKey="Yamashita, Akio" sort="Yamashita, Akio" uniqKey="Yamashita A" first="Akio" last="Yamashita">Akio Yamashita</name><affiliation><nlm:aff id="af3-ijms-18-00676">Department of Molecular Biology, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>yamasita@yokohama-cu.ac.jp</email></nlm:aff></affiliation></author><author><name sortKey="Umemura, Satoshi" sort="Umemura, Satoshi" uniqKey="Umemura S" first="Satoshi" last="Umemura">Satoshi Umemura</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="af4-ijms-18-00676">Yokohama Rosai Hospital, 3211 Kozukue-cho, Kohoku-ku, Yokohama 222-0036, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Yokohama Rosai Hospital, 3211 Kozukue-cho, Kohoku-ku, Yokohama 222-0036</wicri:regionArea><wicri:noRegion>Yokohama 222-0036</wicri:noRegion></affiliation></author><author><name sortKey="Tamura, Kouichi" sort="Tamura, Kouichi" uniqKey="Tamura K" first="Kouichi" last="Tamura">Kouichi Tamura</name><affiliation><nlm:aff id="af1-ijms-18-00676">Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</nlm:aff></affiliation></author></analytic><series><title level="j">International Journal of Molecular Sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Activation of tissue renin–angiotensin system (RAS), mainly mediated by an angiotensin II (Ang II) type 1 receptor (AT1R), plays an important role in the development of obesity-related metabolic disorders. We have shown that AT1R-associated protein (ATRAP), a specific binding protein of AT1R, functions as an endogenous inhibitor to prevent excessive activation of tissue RAS. In the present study, we newly generated ATRAP/<italic>Agtrap</italic>-floxed (ATRAP<sup>fl/fl</sup>) mice and adipose tissue-specific ATRAP downregulated (ATRAP<sup>adipoq</sup>) mice by the Cre/loxP system using <italic>Adipoq-Cre</italic>. Using these mice, we examined the functional role of adipose ATRAP in the pathogenesis of obesity-related metabolic disorders. Compared with ATRAP<sup>fl/fl</sup> mice, ATRAP<sup>adipoq</sup> mice exhibited a decreased ATRAP expression in visceral white adipose tissue (WAT) and brown adipose tissue (BAT) by approximately 30% and 85%, respectively. When mice were fed a high-fat diet, ATRAP<sup>fl/fl</sup> mice showed decreased endogenous ATRAP expression in WAT that was equivalent to ATRAP<sup>adipoq</sup> mice, and there was no difference in the exacerbation of dietary obesity and glucose and lipid metabolism. These results indicate that ATRAP in BAT does not influence the pathogenesis of dietary obesity or metabolic disorders. Future studies that modulate ATRAP in WAT are necessary to assess its in vivo functions in the development of obesity-related metabolic disorders.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, M" uniqKey="Zhang M">M. Zhang</name></author><author><name sortKey="Hu, T" uniqKey="Hu T">T. Hu</name></author><author><name sortKey="Zhang, S" uniqKey="Zhang S">S. Zhang</name></author><author><name sortKey="Zhou, L" uniqKey="Zhou L">L. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Int J Mol Sci</journal-id><journal-id journal-id-type="iso-abbrev">Int J Mol Sci</journal-id><journal-id journal-id-type="publisher-id">ijms</journal-id><journal-title-group><journal-title>International Journal of Molecular Sciences</journal-title></journal-title-group><issn pub-type="epub">1422-0067</issn><publisher><publisher-name>MDPI</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">28335584</article-id><article-id pub-id-type="pmc">5372686</article-id><article-id pub-id-type="doi">10.3390/ijms18030676</article-id><article-id pub-id-type="publisher-id">ijms-18-00676</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>ATRAP Expression in Brown Adipose Tissue Does Not Influence the Development of Diet-Induced Metabolic Disorders in Mice</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Ohki</surname><given-names>Kohji</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref><xref ref-type="author-notes" rid="fn1-ijms-18-00676">†</xref></contrib><contrib contrib-type="author"><name><surname>Wakui</surname><given-names>Hiromichi</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref><xref rid="c1-ijms-18-00676" ref-type="corresp">*</xref><xref ref-type="author-notes" rid="fn1-ijms-18-00676">†</xref></contrib><contrib contrib-type="author"><name><surname>Azushima</surname><given-names>Kengo</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref><xref ref-type="aff" rid="af2-ijms-18-00676">2</xref><xref rid="c1-ijms-18-00676" ref-type="corresp">*</xref><xref ref-type="author-notes" rid="fn1-ijms-18-00676">†</xref></contrib><contrib contrib-type="author"><name><surname>Uneda</surname><given-names>Kazushi</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Haku</surname><given-names>Sona</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Kobayashi</surname><given-names>Ryu</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Haruhara</surname><given-names>Kotaro</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Kinguchi</surname><given-names>Sho</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Matsuda</surname><given-names>Miyuki</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Ohsawa</surname><given-names>Masato</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Maeda</surname><given-names>Akinobu</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Minegishi</surname><given-names>Shintaro</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Ishigami</surname><given-names>Tomoaki</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Toya</surname><given-names>Yoshiyuki</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref></contrib><contrib contrib-type="author"><name><surname>Yamashita</surname><given-names>Akio</given-names></name><xref ref-type="aff" rid="af3-ijms-18-00676">3</xref></contrib><contrib contrib-type="author"><name><surname>Umemura</surname><given-names>Satoshi</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref><xref ref-type="aff" rid="af4-ijms-18-00676">4</xref></contrib><contrib contrib-type="author"><name><surname>Tamura</surname><given-names>Kouichi</given-names></name><xref ref-type="aff" rid="af1-ijms-18-00676">1</xref><xref rid="c1-ijms-18-00676" ref-type="corresp">*</xref></contrib></contrib-group><contrib-group><contrib contrib-type="editor"><name><surname>Mihailidou</surname><given-names>Anastasia Susie</given-names></name><role>Academic Editor</role></contrib><contrib contrib-type="editor"><name><surname>Danser</surname><given-names>Jan</given-names></name><role>Academic Editor</role></contrib><contrib contrib-type="editor"><name><surname>Ito</surname><given-names>Sadayoshi</given-names></name><role>Academic Editor</role></contrib><contrib contrib-type="editor"><name><surname>Satoh</surname><given-names>Fumitoshi</given-names></name><role>Academic Editor</role></contrib><contrib contrib-type="editor"><name><surname>Nishiyama</surname><given-names>Akira</given-names></name><role>Academic Editor</role></contrib></contrib-group><aff id="af1-ijms-18-00676"><label>1</label>Department of Medical Science and Cardiorenal Medicine, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>t146017e@yokohama-cu.ac.jp</email> (K.O.);<email>k_uneda@yokohama-cu.ac.jp</email> (K.U.);<email>t106048f@yokohama-cu.ac.jp</email> (S.H.);<email>t136034d@yokohama-cu.ac.jp</email> (R.K.);<email>kspring00712@gmail.com</email> (K.H.);<email>ja472t@bma.biglobe.ne.jp</email> (S.K.);<email>mmatsuda@yokohama-cu.ac.jp</email> (M.M.);<email>o.masato@gmail.com</email> (M.O.);<email>maeda_chigasaki@yahoo.co.jp</email> (A.M.);<email>minegish@yokohama-cu.ac.jp</email> (S.M.);<email>tommmish@yokohama-cu.ac.jp</email> (T.I.);<email>ystoya@yokohama-cu.ac.jp</email> (Y.T.);<email>umemuras@med.yokohama-cu.ac.jp</email> (S.U.)</aff><aff id="af2-ijms-18-00676"><label>2</label>Cardiovascular and Metabolic Disorders Program, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore</aff><aff id="af3-ijms-18-00676"><label>3</label>Department of Molecular Biology, Yokohama City University Graduate School of Medicine, 3–9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan;<email>yamasita@yokohama-cu.ac.jp</email></aff><aff id="af4-ijms-18-00676"><label>4</label>Yokohama Rosai Hospital, 3211 Kozukue-cho, Kohoku-ku, Yokohama 222-0036, Japan</aff><author-notes><corresp id="c1-ijms-18-00676"><label>*</label>Correspondence: <email>hiro1234@yokohama-cu.ac.jp</email> (H.W.); <email>azushima@yokohama-cu.ac.jp</email> (K.A.); <email>tamukou@med.yokohama-cu.ac.jp</email> (K.T.); Tel.: +81-45-787-2635 (H.W., K.A. & K.T.); Fax: +81-45-701-3738 (H.W., K.A. & K.T.)</corresp><fn id="fn1-ijms-18-00676"><label>†</label><p>These authors contributed equally to this work.</p></fn></author-notes><pub-date pub-type="epub"><day>21</day><month>3</month><year>2017</year></pub-date><pub-date pub-type="collection"><month>3</month><year>2017</year></pub-date><volume>18</volume><issue>3</issue><elocation-id>676</elocation-id><history><date date-type="received"><day>28</day><month>1</month><year>2017</year></date><date date-type="accepted"><day>16</day><month>3</month><year>2017</year></date></history><permissions><copyright-statement>© 2017 by the authors.</copyright-statement><copyright-year>2017</copyright-year><license license-type="open-access"><license-p>Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by/4.0/">http://creativecommons.org/licenses/by/4.0/</ext-link>).</license-p></license></permissions><abstract><p>Activation of tissue renin–angiotensin system (RAS), mainly mediated by an angiotensin II (Ang II) type 1 receptor (AT1R), plays an important role in the development of obesity-related metabolic disorders. We have shown that AT1R-associated protein (ATRAP), a specific binding protein of AT1R, functions as an endogenous inhibitor to prevent excessive activation of tissue RAS. In the present study, we newly generated ATRAP/<italic>Agtrap</italic>-floxed (ATRAP<sup>fl/fl</sup>) mice and adipose tissue-specific ATRAP downregulated (ATRAP<sup>adipoq</sup>) mice by the Cre/loxP system using <italic>Adipoq-Cre</italic>. Using these mice, we examined the functional role of adipose ATRAP in the pathogenesis of obesity-related metabolic disorders. Compared with ATRAP<sup>fl/fl</sup> mice, ATRAP<sup>adipoq</sup> mice exhibited a decreased ATRAP expression in visceral white adipose tissue (WAT) and brown adipose tissue (BAT) by approximately 30% and 85%, respectively. When mice were fed a high-fat diet, ATRAP<sup>fl/fl</sup> mice showed decreased endogenous ATRAP expression in WAT that was equivalent to ATRAP<sup>adipoq</sup> mice, and there was no difference in the exacerbation of dietary obesity and glucose and lipid metabolism. These results indicate that ATRAP in BAT does not influence the pathogenesis of dietary obesity or metabolic disorders. Future studies that modulate ATRAP in WAT are necessary to assess its in vivo functions in the development of obesity-related metabolic disorders.</p></abstract><kwd-group><kwd>Cre/loxP</kwd><kwd>angiotensin</kwd><kwd>receptor</kwd><kwd>adipocyte</kwd><kwd>obesity</kwd></kwd-group></article-meta></front><floats-group><fig id="ijms-18-00676-f001" position="float"><label>Figure 1</label><caption><p>Generation of adipose tissue-specific angiotensin II type-1 receptor-associated protein (ATRAP) downregulated mice. (<bold>A</bold>) Schematic representation of <italic>Adipoq-Cre</italic> mediated recombination in adipocytes: (<bold>a</bold>) wild-type allele; (<bold>b</bold>) targeting vector; (<bold>c</bold>) targeted allele; (<bold>d</bold>) Flp recombinase-mediated allele; and (<bold>e</bold>) <italic>Cre</italic>-mediated allele. (<bold>B</bold>) Agarose-gel electrophoresis of PCR products amplified with following primer sets, (<bold>a</bold>) F23005 and R23744, (<bold>b</bold>) F24587 and R24917, and (<bold>c</bold>) Cre-fw and Cre-rw for <italic>Cre</italic> transgene, mAgtrapChIPF and mAgtrapChIPR for <italic>Agtrap</italic> gene as an internal control. These primer sequences are described in <xref ref-type="table" rid="ijms-18-00676-t001">Table 1</xref>. (<bold>C</bold>) The relative ATRAP mRNA levels in each tissue (WAT, BAT, liver, muscle, kidney, heart, and brain) of ATRAP<sup>adipoq</sup> and ATRAP<sup>fl/fl</sup> mice aged 19–24 weeks on an LFD (<italic>n</italic> = 3–10). (<bold>D</bold>) Representative Western blots of ATRAP protein expression in WAT and BAT of ATRAP<sup>fl/fl</sup> mice on an LFD. Values are the means ± standard error of the mean (SEM). <sup>†</sup><italic>p</italic> < 0.05, <sup>††</sup><italic>p</italic> < 0.01 vs. ATRAP<sup>fl/fl</sup> mice. Data were analyzed by unpaired Student’s <italic>t</italic>-test. EX: Exon; <italic>PGK-Neo</italic>: Phosphoglycerate kinase 1 promoter driven neomycin resistant gene; <italic>PGK-TK</italic>: Phosphoglycerate kinase 1 promoter driven SV40 thymidine kinase gene; WAT: White adipose tissue; BAT: Brown adipose tissue; LFD: Low-fat diet.</p></caption><graphic xlink:href="ijms-18-00676-g001a"></graphic><graphic xlink:href="ijms-18-00676-g001b"></graphic></fig><fig id="ijms-18-00676-f002" position="float"><label>Figure 2</label><caption><p>The relative expression of renin–angiotensin system (RAS) components in adipose tissues. Relative angiotensin II type-1 receptor (AT1R) mRNA (<bold>A</bold>) and angiotensinogen mRNA (<bold>B</bold>) expression in WAT and BAT of ATRAP<sup>fl/fl</sup> and ATRAP<sup>adipoq</sup> mice fed an LFD (<italic>n</italic> = 5–6). Values are the means ± standard error of the mean (SEM). AT1R: Angiotensin II type1 receptor; WAT: White adipose tissue; BAT: Brown adipose tissue; LFD: Low-fat diet.</p></caption><graphic xlink:href="ijms-18-00676-g002"></graphic></fig><fig id="ijms-18-00676-f003" position="float"><label>Figure 3</label><caption><p>Change in body weight in ATRAP<sup>fl/fl</sup> and ATRAP<sup>adipoq</sup> mice fed an LFD or HFD (<italic>n</italic> = 9). ∆, ATRAP<sup>fl/fl</sup> mice fed an LFD; ▲, ATRAP<sup>adipoq</sup> mice fed an LFD; ○, ATRAP<sup>fl/fl</sup> mice fed an HFD; ●, ATRAP<sup>adipoq</sup> mice fed an HFD. Values are the means ± SEM. *** <italic>p</italic> < 0.001 vs. LFD within the same group. Data were analyzed by two-way repeated measures analysis of variance (ANOVA). LFD: Low-fat diet; HFD: High-fat diet.</p></caption><graphic xlink:href="ijms-18-00676-g003"></graphic></fig><fig id="ijms-18-00676-f004" position="float"><label>Figure 4</label><caption><p>Glucose tolerance test (GTT) and insulin tolerance test (ITT). (<bold>A</bold>,<bold>B</bold>) The GTT and ITT in ATRAP<sup>fl/fl</sup> and ATRAP<sup>adipoq</sup> mice after 16-week LFD or HFD feeding (<italic>n</italic> = 5–15). ∆, ATRAP<sup>fl/fl</sup> mice fed an LFD; ▲, ATRAP<sup>adipoq</sup> mice fed an LFD; ○, ATRAP<sup>fl/fl</sup> mice fed an HFD; ●, ATRAP<sup>adipoq</sup> mice fed an HFD; (<bold>C</bold>,<bold>D</bold>) The area under the curve (AUC) of GTT and ITT in ATRAP<sup>fl/fl</sup> and ATRAP<sup>adipoq</sup> mice after 16-week LFD or HFD feeding (<italic>n</italic> = 5–15). Values are the means ± SEM. (<bold>A</bold>,<bold>B</bold>) *** <italic>p</italic> < 0.001 vs. LFD within the same group. Data were analyzed by two-way repeated measures ANOVA. (<bold>C</bold>,<bold>D</bold>) *** <italic>p</italic> < 0.001 vs. LFD within the same group. Data were analyzed by two-way ANOVA. LFD: Low-fat diet; HFD: High-fat diet.</p></caption><graphic xlink:href="ijms-18-00676-g004"></graphic></fig><fig id="ijms-18-00676-f005" position="float"><label>Figure 5</label><caption><p>Adipocyte morphology and inflammation of WAT. (<bold>A</bold>) Representative images of adipocyte cells in WAT of ATRAP<sup>fl/fl</sup> and ATRAP<sup>adipoq</sup> mice fed an LFD and HFD. (<bold>B</bold>) Relative monocyte chemotactic protein-1 (MCP-1) and F4/80 mRNA expression in WAT of ATRAP<sup>fl/fl</sup> and ATRAP<sup>adipoq</sup> mice fed an LFD and HFD (<italic>n</italic> = 5–6). Values are the means ± SEM. * <italic>p</italic> < 0.05, ** <italic>p</italic> < 0.01, *** <italic>p</italic> < 0.001 vs. LFD within the same group. Data were analyzed by two-way ANOVA. LFD: Low-fat diet; HFD: High-fat diet.</p></caption><graphic xlink:href="ijms-18-00676-g005"></graphic></fig><fig id="ijms-18-00676-f006" position="float"><label>Figure 6</label><caption><p>The changes of ATRAP expression in adipose tissues and liver with HFD. (<bold>A</bold>) Relative WAT ATRAP mRNA expression in ATRAP<sup>fl/fl</sup> and ATRAP<sup>adipoq</sup> mice fed an LFD and HFD (<italic>n</italic> = 6). (<bold>B</bold>) Relative BAT ATRAP mRNA expression in ATRAP<sup>fl/fl</sup> and ATRAP<sup>adipoq</sup> mice fed an LFD and HFD (<italic>n</italic> = 6). Relative liver ATRAP mRNA expression in ATRAP<sup>fl/fl</sup> mice fed an LFD and HFD (<italic>n</italic> = 6). Values are the means ± SEM. (A, B) <sup>†</sup><italic>p</italic> < 0.05, <sup>†††</sup><italic>p</italic> < 0.001 vs. ATRAP<sup>fl/fl</sup> mice on the same diet. * <italic>p</italic> < 0.05, *** <italic>p</italic> < 0.001 vs. LFD within the same group. Data were analyzed by two-way ANOVA. (<bold>C</bold>) * <italic>p</italic> < 0.05 vs. LFD group. Data were analyzed by an unpaired Student’s <italic>t</italic>-test. LFD: Low-fat diet; HFD: High-fat diet.</p></caption><graphic xlink:href="ijms-18-00676-g006"></graphic></fig><fig id="ijms-18-00676-f007" position="float"><label>Figure 7</label><caption><p>Agarose-gel electrophoresis of PCR products from embryonic stem (ES) cell clones’ DNA (#a2925–a2930, #a3006–a3020), amplified for homologous recombination (<bold>A</bold>) and for detecting second <italic>loxP</italic> (<bold>B</bold>). (<bold>A</bold>) 5′ PCR using primer set: 5AF4/neo100 (upper panel) and 3′ PCR using primer set: neo marker sense/3AR2 (lower panel); red-labeled clones were positive for amplification. (<bold>B</bold>) PCR using primer set: F24587/R2491; red-labeled clones were positive for amplification of mutant <italic>loxP</italic> within positive for homologous recombination.</p></caption><graphic xlink:href="ijms-18-00676-g007"></graphic></fig><fig id="ijms-18-00676-f008" position="float"><label>Figure 8</label><caption><p>Southern blot analysis using neo probe. 5′ EcoRI-digested (target mutant band is 8.3 kb in upper panel) and 3′ NdeI-digested (target mutant band is 10.3 kb in lower panel) DNA were analyzed. #a3006 and #a3008 clones (in red) were verified to have one inserted copy of the targeting vector.</p></caption><graphic xlink:href="ijms-18-00676-g008"></graphic></fig><fig id="ijms-18-00676-f009" position="float"><label>Figure 9</label><caption><p>Agarose-gel electrophoresis of PCR products amplified to detect first <italic>loxP</italic> using the primer set F23005 and R23744. Analyzed DNAs (F3-1–F3-15) were from F3 mice with heterozygous <italic>Agtrap</italic> floxed allele from which the <italic>PGK-Neo</italic> cassette was removed. The red-labeled sample were positive for <italic>loxP</italic> site amplification.</p></caption><graphic xlink:href="ijms-18-00676-g009"></graphic></fig><table-wrap id="ijms-18-00676-t001" position="float"><object-id pub-id-type="pii">ijms-18-00676-t001_Table 1</object-id><label>Table 1</label><caption><p>Primer sequences to generate <italic>Agtrap<sup>fl/fl</sup></italic> and <italic>Adipoq-Cre<sup>+</sup>/Agtrap<sup>fl/fl</sup></italic> mice.</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" valign="middle" style="border-top:solid thin;border-bottom:solid thin" rowspan="1" colspan="1">Primer</th><th align="left" valign="middle" style="border-top:solid thin;border-bottom:solid thin" rowspan="1" colspan="1">Sequence (5′–3′)</th><th align="left" valign="middle" style="border-top:solid thin;border-bottom:solid thin" rowspan="1" colspan="1">Target Region</th></tr></thead><tbody><tr><td align="left" valign="middle" rowspan="1" colspan="1">F23005</td><td align="left" valign="middle" rowspan="1" colspan="1">CCTCTTCTGGACCACTCTATCTCTCTGC</td><td rowspan="2" align="left" valign="middle" style="border-bottom:solid thin" colspan="1">first <italic>loxP</italic></td></tr><tr><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">R23744</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">GTTCCAGGGTCTTAACCTCCTCTGAG</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">F24587</td><td align="left" valign="middle" rowspan="1" colspan="1">CTCGTCTACCACATGCACCGTCAACG</td><td rowspan="2" align="left" valign="middle" style="border-bottom:solid thin" colspan="1">second <italic>loxP</italic></td></tr><tr><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">R24917</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">AGCTCCCATAGAATAGGTTCAGAGAGG</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">cre-fw</td><td align="left" valign="middle" rowspan="1" colspan="1">AGGTTCGTGCACTCATGGA</td><td rowspan="2" align="left" valign="middle" style="border-bottom:solid thin" colspan="1"><italic>cre</italic></td></tr><tr><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">cre-rw</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">TCGACCAGTTTAGTTACCC</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">mAgtrapChIPF</td><td align="left" valign="middle" rowspan="1" colspan="1">CCTAGCAGCAAGAGCAGCT</td><td rowspan="2" align="left" valign="middle" style="border-bottom:solid thin" colspan="1"><italic>agtrap</italic></td></tr><tr><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">mAgtrapChIPR</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">GAACTCGGGAACAAACTTCCT</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">5AF5</td><td align="left" valign="middle" rowspan="1" colspan="1">AGTGAATTCATTATCTAGGGACAGAATTACAGG</td><td rowspan="2" align="left" valign="middle" style="border-bottom:solid thin" colspan="1">5′-targeted allele (1st)</td></tr><tr><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">neo108r</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">CCTCAGAAGAACTCGTCAAGAAG</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">5AF4</td><td align="left" valign="middle" rowspan="1" colspan="1">AGTGAATTCAGGTCAGGCTCTGCCTTATTCTGC</td><td rowspan="2" align="left" valign="middle" style="border-bottom:solid thin" colspan="1">5′-targeted allele (nested)</td></tr><tr><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">neo100</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">AGGTGAGATGACAGGAGATC</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">neo_marker_sense</td><td align="left" valign="middle" rowspan="1" colspan="1">ATTCGCAGCGCATCGCCTTCTATCGCCTTC</td><td rowspan="2" align="left" valign="middle" style="border-bottom:solid thin" colspan="1">3′-targeted allele</td></tr><tr><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">3AR2</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">TAAGCGGCCGCTCTCCCCAGAAATAGCTGGAAATCACC</td></tr></tbody></table></table-wrap><table-wrap id="ijms-18-00676-t002" position="float"><object-id pub-id-type="pii">ijms-18-00676-t002_Table 2</object-id><label>Table 2</label><caption><p>Physiological and metabolic parameters of mice on a low-fat diet or high-fat diet.</p></caption><table frame="hsides" rules="groups"><thead><tr><th rowspan="2" align="left" valign="middle" style="border-top:solid thin;border-bottom:solid thin" colspan="1">Variables</th><th colspan="2" align="center" valign="middle" style="border-top:solid thin;border-bottom:solid thin" rowspan="1">LFD</th><th colspan="2" align="center" valign="middle" style="border-top:solid thin;border-bottom:solid thin" rowspan="1">HFD</th></tr><tr><th align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">ATRAP<sup>fl/fl</sup></th><th align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">ATRAP<sup>adipoq</sup></th><th align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">ATRAP<sup>fl/fl</sup></th><th align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">ATRAP<sup>adipoq</sup></th></tr></thead><tbody><tr><td align="left" valign="middle" rowspan="1" colspan="1">Body weight (g)</td><td align="left" valign="middle" rowspan="1" colspan="1">31.6 ± 0.6</td><td align="left" valign="middle" rowspan="1" colspan="1">29.5 ± 0.5</td><td align="left" valign="middle" rowspan="1" colspan="1">48.9 ± 0.8 ***</td><td align="left" valign="middle" rowspan="1" colspan="1">49.1 ± 1.1 ***</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Systolic blood pressure (mmHg)</td><td align="left" valign="middle" rowspan="1" colspan="1">114 ± 1</td><td align="left" valign="middle" rowspan="1" colspan="1">116 ± 1</td><td align="left" valign="middle" rowspan="1" colspan="1">124 ± 2 *</td><td align="left" valign="middle" rowspan="1" colspan="1">124 ± 3</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Heart rate (beat/min)</td><td align="left" valign="middle" rowspan="1" colspan="1">742 ± 1</td><td align="left" valign="middle" rowspan="1" colspan="1">747 ± 15</td><td align="left" valign="middle" rowspan="1" colspan="1">712 ± 8</td><td align="left" valign="middle" rowspan="1" colspan="1">743 ± 11</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Rectal temperature (°C)</td><td align="left" valign="middle" rowspan="1" colspan="1">37.2 ± 0.2</td><td align="left" valign="middle" rowspan="1" colspan="1">37.8 ± 0.3</td><td align="left" valign="middle" rowspan="1" colspan="1">37.8 ± 0.1</td><td align="left" valign="middle" rowspan="1" colspan="1">37.7 ± 0.1</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Tissue weight</td><td align="left" valign="middle" rowspan="1" colspan="1"></td><td align="left" valign="middle" rowspan="1" colspan="1"></td><td align="left" valign="middle" rowspan="1" colspan="1"></td><td align="left" valign="middle" rowspan="1" colspan="1"></td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Epididymal adipose tissue (mg)</td><td align="left" valign="middle" rowspan="1" colspan="1">872 ± 160</td><td align="left" valign="middle" rowspan="1" colspan="1">694 ± 144</td><td align="left" valign="middle" rowspan="1" colspan="1">1293 ± 25 ***</td><td align="left" valign="middle" rowspan="1" colspan="1">1220 ± 48 ***</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Brown adipose tissue (mg)</td><td align="left" valign="middle" rowspan="1" colspan="1">139 ± 14</td><td align="left" valign="middle" rowspan="1" colspan="1">128 ± 7</td><td align="left" valign="middle" rowspan="1" colspan="1">425 ± 27 ***</td><td align="left" valign="middle" rowspan="1" colspan="1">404 ± 22 ***</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Liver (mg)</td><td align="left" valign="middle" rowspan="1" colspan="1">1510 ± 127</td><td align="left" valign="middle" rowspan="1" colspan="1">1407 ± 50</td><td align="left" valign="middle" rowspan="1" colspan="1">3670 ± 226 ***</td><td align="left" valign="middle" rowspan="1" colspan="1">3242 ± 192 ***</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Heart (mg)</td><td align="left" valign="middle" rowspan="1" colspan="1">148 ± 9</td><td align="left" valign="middle" rowspan="1" colspan="1">139 ± 7</td><td align="left" valign="middle" rowspan="1" colspan="1">156 ± 6</td><td align="left" valign="middle" rowspan="1" colspan="1">145 ± 4</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Plasma concentration</td><td align="left" valign="middle" rowspan="1" colspan="1"></td><td align="left" valign="middle" rowspan="1" colspan="1"></td><td align="left" valign="middle" rowspan="1" colspan="1"></td><td align="left" valign="middle" rowspan="1" colspan="1"></td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Total cholesterol (mg/dL)</td><td align="left" valign="middle" rowspan="1" colspan="1">83 ± 8</td><td align="left" valign="middle" rowspan="1" colspan="1">91 ± 6</td><td align="left" valign="middle" rowspan="1" colspan="1">250 ± 16 ***</td><td align="left" valign="middle" rowspan="1" colspan="1">218 ± 12 ***</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Triglyceride (mg/dL)</td><td align="left" valign="middle" rowspan="1" colspan="1">35 ± 9</td><td align="left" valign="middle" rowspan="1" colspan="1">38 ± 8</td><td align="left" valign="middle" rowspan="1" colspan="1">35 ± 4</td><td align="left" valign="middle" rowspan="1" colspan="1">22 ± 1</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Non-esterified fatty acid (µEq/L)</td><td align="left" valign="middle" rowspan="1" colspan="1">470 ± 147</td><td align="left" valign="middle" rowspan="1" colspan="1">496 ± 143</td><td align="left" valign="middle" rowspan="1" colspan="1">456 ± 94</td><td align="left" valign="middle" rowspan="1" colspan="1">241 ± 42</td></tr><tr><td align="left" valign="middle" rowspan="1" colspan="1">Glucose (mg/dL)</td><td align="left" valign="middle" rowspan="1" colspan="1">232 ± 16</td><td align="left" valign="middle" rowspan="1" colspan="1">206 ± 11</td><td align="left" valign="middle" rowspan="1" colspan="1">259 ± 19</td><td align="left" valign="middle" rowspan="1" colspan="1">288 ± 25 *</td></tr><tr><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">Insulin (ng/mL)</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">1.3 ± 0.2</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">1.3 ± 0.1</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">8.1 ± 1.7 **</td><td align="left" valign="middle" style="border-bottom:solid thin" rowspan="1" colspan="1">7.1 ± 2.2 *</td></tr></tbody></table><table-wrap-foot><fn><p>All of the values are the means ± SEM. * <italic>p</italic> < 0.05, ** <italic>p</italic> < 0.01, *** <italic>p</italic> < 0.001 vs. LFD within the same group. <italic>n</italic> = 6–12 per group. Data were analyzed by two-way ANOVA. ATRAP<sup>fl/fl</sup>: <italic>Agtrap</italic>-floxed control mice; ATRAP<sup>adipoq</sup>: <italic>Agtrap</italic>-floxed with <italic>Adipoq-Cre</italic> transgenic mice; LFD: Low-fat diet; HFD: High-fat diet.</p></fn></table-wrap-foot></table-wrap></floats-group></pmc><affiliations><list><country><li>Japon</li><li>Singapour</li></country></list><tree><noCountry><name sortKey="Haku, Sona" sort="Haku, Sona" uniqKey="Haku S" first="Sona" last="Haku">Sona Haku</name><name sortKey="Haruhara, Kotaro" sort="Haruhara, Kotaro" uniqKey="Haruhara K" first="Kotaro" last="Haruhara">Kotaro Haruhara</name><name sortKey="Ishigami, Tomoaki" sort="Ishigami, Tomoaki" uniqKey="Ishigami T" first="Tomoaki" last="Ishigami">Tomoaki Ishigami</name><name sortKey="Kinguchi, Sho" sort="Kinguchi, Sho" uniqKey="Kinguchi S" first="Sho" last="Kinguchi">Sho Kinguchi</name><name sortKey="Kobayashi, Ryu" sort="Kobayashi, Ryu" uniqKey="Kobayashi R" first="Ryu" last="Kobayashi">Ryu Kobayashi</name><name sortKey="Maeda, Akinobu" sort="Maeda, Akinobu" uniqKey="Maeda A" first="Akinobu" last="Maeda">Akinobu Maeda</name><name sortKey="Matsuda, Miyuki" sort="Matsuda, Miyuki" uniqKey="Matsuda M" first="Miyuki" last="Matsuda">Miyuki Matsuda</name><name sortKey="Minegishi, Shintaro" sort="Minegishi, Shintaro" uniqKey="Minegishi S" first="Shintaro" last="Minegishi">Shintaro Minegishi</name><name sortKey="Ohki, Kohji" sort="Ohki, Kohji" uniqKey="Ohki K" first="Kohji" last="Ohki">Kohji Ohki</name><name sortKey="Ohsawa, Masato" sort="Ohsawa, Masato" uniqKey="Ohsawa M" first="Masato" last="Ohsawa">Masato Ohsawa</name><name sortKey="Tamura, Kouichi" sort="Tamura, Kouichi" uniqKey="Tamura K" first="Kouichi" last="Tamura">Kouichi Tamura</name><name sortKey="Toya, Yoshiyuki" sort="Toya, Yoshiyuki" uniqKey="Toya Y" first="Yoshiyuki" last="Toya">Yoshiyuki Toya</name><name sortKey="Uneda, Kazushi" sort="Uneda, Kazushi" uniqKey="Uneda K" first="Kazushi" last="Uneda">Kazushi Uneda</name><name sortKey="Wakui, Hiromichi" sort="Wakui, Hiromichi" uniqKey="Wakui H" first="Hiromichi" last="Wakui">Hiromichi Wakui</name><name sortKey="Yamashita, Akio" sort="Yamashita, Akio" uniqKey="Yamashita A" first="Akio" last="Yamashita">Akio Yamashita</name></noCountry><country name="Singapour"><noRegion><name sortKey="Azushima, Kengo" sort="Azushima, Kengo" uniqKey="Azushima K" first="Kengo" last="Azushima">Kengo Azushima</name></noRegion></country><country name="Japon"><noRegion><name sortKey="Umemura, Satoshi" sort="Umemura, Satoshi" uniqKey="Umemura S" first="Satoshi" last="Umemura">Satoshi Umemura</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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