Measurement of Circulating Filarial Antigen Levels in Human Blood with a Point-of-Care Test Strip and a Portable Spectrodensitometer.
Identifieur interne : 008214 ( Ncbi/Merge ); précédent : 008213; suivant : 008215Measurement of Circulating Filarial Antigen Levels in Human Blood with a Point-of-Care Test Strip and a Portable Spectrodensitometer.
Auteurs : Cédric B. Chesnais [Oman] ; Johnny Vlaminck ; Billy Kunyu-Shako ; Sébastien D. Pion ; Naomi-Pitchouna Awaca-Uvon ; Gary J. Weil ; Dieudonné Mumba ; Michel BoussinesqSource :
- The American journal of tropical medicine and hygiene [ 1476-1645 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Wuchereria bancrofti.
- métabolisme : Wuchereria bancrofti.
- sang : Antigènes d'helminthe.
- Analyse sur le lieu d'intervention, Animaux, Bandelettes réactives, Humains, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antigens, Helminth.
- diagnosis : Elephantiasis, Filarial.
- immunology : Wuchereria bancrofti.
- metabolism : Wuchereria bancrofti.
- Animals, Humans, Point-of-Care Testing, Reagent Strips, Sensitivity and Specificity.
Abstract
The Alere Filariasis Test Strip (FTS) is a qualitative, point-of-care diagnostic tool that detects Wuchereria bancrofti circulating filarial antigen (CFA) in human blood, serum, or plasma. The Global Program to Eliminate Lymphatic Filariasis employs the FTS for mapping filariasis-endemic areas and assessing the success of elimination efforts. The objective of this study was to explore the relationship between the intensity of positive test lines obtained by FTS with CFA levels as determined by enzyme-linked immunosorbent assay (ELISA) with blood and plasma samples from 188 individuals who live in a filariasis-endemic area. The intensity of the FTS test line was assessed visually to provide a semiquantitative score (visual Filariasis Test Strip [vFTS]), and line intensity was measured with a portable spectrodensitometer (quantitative Filariasis Test Strip [qFTS]). These results were compared with antigen levels measured by ELISA in plasma from the same subjects. qFTS measurements were highly correlated with vFTS scores (ρ = 0.94; P < 0.001) and with plasma CFA levels (ρ = 0.91; P < 0.001). Thus, qFTS assessment is a convenient method for quantifying W. bancrofti CFA levels in human blood, which are correlated with adult worm burdens. This tool may be useful for assessing the impact of treatment on adult filarial worms in individuals and communities.
DOI: 10.4269/ajtmh.15-0916
PubMed: 27114288
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000860
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pubmed:27114288Le document en format XML
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<author><name sortKey="Boussinesq, Michel" sort="Boussinesq, Michel" uniqKey="Boussinesq M" first="Michel" last="Boussinesq">Michel Boussinesq</name>
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<term>Humans</term>
<term>Point-of-Care Testing</term>
<term>Reagent Strips</term>
<term>Sensitivity and Specificity</term>
<term>Wuchereria bancrofti (immunology)</term>
<term>Wuchereria bancrofti (metabolism)</term>
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<term>Animaux</term>
<term>Antigènes d'helminthe (sang)</term>
<term>Bandelettes réactives</term>
<term>Filariose lymphatique (diagnostic)</term>
<term>Humains</term>
<term>Sensibilité et spécificité</term>
<term>Wuchereria bancrofti (immunologie)</term>
<term>Wuchereria bancrofti (métabolisme)</term>
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</keywords>
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</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes d'helminthe</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Humans</term>
<term>Point-of-Care Testing</term>
<term>Reagent Strips</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse sur le lieu d'intervention</term>
<term>Animaux</term>
<term>Bandelettes réactives</term>
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<front><div type="abstract" xml:lang="en">The Alere Filariasis Test Strip (FTS) is a qualitative, point-of-care diagnostic tool that detects Wuchereria bancrofti circulating filarial antigen (CFA) in human blood, serum, or plasma. The Global Program to Eliminate Lymphatic Filariasis employs the FTS for mapping filariasis-endemic areas and assessing the success of elimination efforts. The objective of this study was to explore the relationship between the intensity of positive test lines obtained by FTS with CFA levels as determined by enzyme-linked immunosorbent assay (ELISA) with blood and plasma samples from 188 individuals who live in a filariasis-endemic area. The intensity of the FTS test line was assessed visually to provide a semiquantitative score (visual Filariasis Test Strip [vFTS]), and line intensity was measured with a portable spectrodensitometer (quantitative Filariasis Test Strip [qFTS]). These results were compared with antigen levels measured by ELISA in plasma from the same subjects. qFTS measurements were highly correlated with vFTS scores (ρ = 0.94; P < 0.001) and with plasma CFA levels (ρ = 0.91; P < 0.001). Thus, qFTS assessment is a convenient method for quantifying W. bancrofti CFA levels in human blood, which are correlated with adult worm burdens. This tool may be useful for assessing the impact of treatment on adult filarial worms in individuals and communities.</div>
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<ArticleTitle>Measurement of Circulating Filarial Antigen Levels in Human Blood with a Point-of-Care Test Strip and a Portable Spectrodensitometer.</ArticleTitle>
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<Abstract><AbstractText>The Alere Filariasis Test Strip (FTS) is a qualitative, point-of-care diagnostic tool that detects Wuchereria bancrofti circulating filarial antigen (CFA) in human blood, serum, or plasma. The Global Program to Eliminate Lymphatic Filariasis employs the FTS for mapping filariasis-endemic areas and assessing the success of elimination efforts. The objective of this study was to explore the relationship between the intensity of positive test lines obtained by FTS with CFA levels as determined by enzyme-linked immunosorbent assay (ELISA) with blood and plasma samples from 188 individuals who live in a filariasis-endemic area. The intensity of the FTS test line was assessed visually to provide a semiquantitative score (visual Filariasis Test Strip [vFTS]), and line intensity was measured with a portable spectrodensitometer (quantitative Filariasis Test Strip [qFTS]). These results were compared with antigen levels measured by ELISA in plasma from the same subjects. qFTS measurements were highly correlated with vFTS scores (ρ = 0.94; P < 0.001) and with plasma CFA levels (ρ = 0.91; P < 0.001). Thus, qFTS assessment is a convenient method for quantifying W. bancrofti CFA levels in human blood, which are correlated with adult worm burdens. This tool may be useful for assessing the impact of treatment on adult filarial worms in individuals and communities.</AbstractText>
<CopyrightInformation>© The American Society of Tropical Medicine and Hygiene.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chesnais</LastName>
<ForeName>Cédric B</ForeName>
<Initials>CB</Initials>
<AffiliationInfo><Affiliation>Unité Mixte Internationale (UMI) 233, Institut de Recherche pour le Développement (IRD), Montpellier, France; Université Montpellier, Montpellier, France; Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1175, Montpellier, France; Infectious Diseases Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri; Department of Parasitology, National Institute of Biomedical Research (INRB), Kinshasa, Democratic Republic of the Congo; National Onchocerciasis Control Programme, Ministry of Public Health, Kinshasa, Democratic Republic of the Congo cedric.chesnais@gmail.com.</Affiliation>
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</Author>
<Author ValidYN="Y"><LastName>Vlaminck</LastName>
<ForeName>Johnny</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Unité Mixte Internationale (UMI) 233, Institut de Recherche pour le Développement (IRD), Montpellier, France; Université Montpellier, Montpellier, France; Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1175, Montpellier, France; Infectious Diseases Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri; Department of Parasitology, National Institute of Biomedical Research (INRB), Kinshasa, Democratic Republic of the Congo; National Onchocerciasis Control Programme, Ministry of Public Health, Kinshasa, Democratic Republic of the Congo.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kunyu-Shako</LastName>
<ForeName>Billy</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>Unité Mixte Internationale (UMI) 233, Institut de Recherche pour le Développement (IRD), Montpellier, France; Université Montpellier, Montpellier, France; Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1175, Montpellier, France; Infectious Diseases Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri; Department of Parasitology, National Institute of Biomedical Research (INRB), Kinshasa, Democratic Republic of the Congo; National Onchocerciasis Control Programme, Ministry of Public Health, Kinshasa, Democratic Republic of the Congo.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Pion</LastName>
<ForeName>Sébastien D</ForeName>
<Initials>SD</Initials>
<AffiliationInfo><Affiliation>Unité Mixte Internationale (UMI) 233, Institut de Recherche pour le Développement (IRD), Montpellier, France; Université Montpellier, Montpellier, France; Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1175, Montpellier, France; Infectious Diseases Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri; Department of Parasitology, National Institute of Biomedical Research (INRB), Kinshasa, Democratic Republic of the Congo; National Onchocerciasis Control Programme, Ministry of Public Health, Kinshasa, Democratic Republic of the Congo.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Awaca-Uvon</LastName>
<ForeName>Naomi-Pitchouna</ForeName>
<Initials>NP</Initials>
<AffiliationInfo><Affiliation>Unité Mixte Internationale (UMI) 233, Institut de Recherche pour le Développement (IRD), Montpellier, France; Université Montpellier, Montpellier, France; Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1175, Montpellier, France; Infectious Diseases Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri; Department of Parasitology, National Institute of Biomedical Research (INRB), Kinshasa, Democratic Republic of the Congo; National Onchocerciasis Control Programme, Ministry of Public Health, Kinshasa, Democratic Republic of the Congo.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Weil</LastName>
<ForeName>Gary J</ForeName>
<Initials>GJ</Initials>
<AffiliationInfo><Affiliation>Unité Mixte Internationale (UMI) 233, Institut de Recherche pour le Développement (IRD), Montpellier, France; Université Montpellier, Montpellier, France; Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1175, Montpellier, France; Infectious Diseases Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri; Department of Parasitology, National Institute of Biomedical Research (INRB), Kinshasa, Democratic Republic of the Congo; National Onchocerciasis Control Programme, Ministry of Public Health, Kinshasa, Democratic Republic of the Congo.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mumba</LastName>
<ForeName>Dieudonné</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Unité Mixte Internationale (UMI) 233, Institut de Recherche pour le Développement (IRD), Montpellier, France; Université Montpellier, Montpellier, France; Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1175, Montpellier, France; Infectious Diseases Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri; Department of Parasitology, National Institute of Biomedical Research (INRB), Kinshasa, Democratic Republic of the Congo; National Onchocerciasis Control Programme, Ministry of Public Health, Kinshasa, Democratic Republic of the Congo.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Boussinesq</LastName>
<ForeName>Michel</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Unité Mixte Internationale (UMI) 233, Institut de Recherche pour le Développement (IRD), Montpellier, France; Université Montpellier, Montpellier, France; Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 1175, Montpellier, France; Infectious Diseases Division, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri; Department of Parasitology, National Institute of Biomedical Research (INRB), Kinshasa, Democratic Republic of the Congo; National Onchocerciasis Control Programme, Ministry of Public Health, Kinshasa, Democratic Republic of the Congo.</Affiliation>
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<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>04</Month>
<Day>25</Day>
</ArticleDate>
</Article>
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<MedlineTA>Am J Trop Med Hyg</MedlineTA>
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<ISSNLinking>0002-9637</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011934">Reagent Strips</NameOfSubstance>
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</ChemicalList>
<CitationSubset>AIM</CitationSubset>
<CitationSubset>IM</CitationSubset>
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</CommentsCorrectionsList>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000947" MajorTopicYN="N">Antigens, Helminth</DescriptorName>
<QualifierName UI="Q000097" MajorTopicYN="Y">blood</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004605" MajorTopicYN="N">Elephantiasis, Filarial</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000067716" MajorTopicYN="Y">Point-of-Care Testing</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011934" MajorTopicYN="Y">Reagent Strips</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014958" MajorTopicYN="N">Wuchereria bancrofti</DescriptorName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
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<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year>
<Month>12</Month>
<Day>22</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>02</Month>
<Day>01</Day>
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<PubMedPubDate PubStatus="entrez"><Year>2016</Year>
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<affiliations><list><country><li>Oman</li>
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<name sortKey="Boussinesq, Michel" sort="Boussinesq, Michel" uniqKey="Boussinesq M" first="Michel" last="Boussinesq">Michel Boussinesq</name>
<name sortKey="Kunyu Shako, Billy" sort="Kunyu Shako, Billy" uniqKey="Kunyu Shako B" first="Billy" last="Kunyu-Shako">Billy Kunyu-Shako</name>
<name sortKey="Mumba, Dieudonne" sort="Mumba, Dieudonne" uniqKey="Mumba D" first="Dieudonné" last="Mumba">Dieudonné Mumba</name>
<name sortKey="Pion, Sebastien D" sort="Pion, Sebastien D" uniqKey="Pion S" first="Sébastien D" last="Pion">Sébastien D. Pion</name>
<name sortKey="Vlaminck, Johnny" sort="Vlaminck, Johnny" uniqKey="Vlaminck J" first="Johnny" last="Vlaminck">Johnny Vlaminck</name>
<name sortKey="Weil, Gary J" sort="Weil, Gary J" uniqKey="Weil G" first="Gary J" last="Weil">Gary J. Weil</name>
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<country name="Oman"><noRegion><name sortKey="Chesnais, Cedric B" sort="Chesnais, Cedric B" uniqKey="Chesnais C" first="Cédric B" last="Chesnais">Cédric B. Chesnais</name>
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