Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity
Identifieur interne : 008016 ( Ncbi/Merge ); précédent : 008015; suivant : 008017Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity
Auteurs : Timothy Kassis [Géorgie (pays)] ; Sri Charan Yarlagadda [Géorgie (pays)] ; Alison B. Kohan [États-Unis] ; Patrick Tso [États-Unis] ; Victor Breedveld [Géorgie (pays)] ; J. Brandon Dixon [Géorgie (pays)]Source :
- American Journal of Physiology - Gastrointestinal and Liver Physiology [ 0193-1857 ] ; 2016.
Abstract
Dietary lipids are transported from the intestine through contractile lymphatics. Chronic lipid loads can adversely affect lymphatic function. However, the acute lymphatic pump response in the mesentery to a postprandial lipid meal has gone unexplored. In this study, we used the rat mesenteric collecting vessel as an in vivo model to quantify the effect of lipoproteins on vessel function. Lipid load was continuously monitored by using the intensity of a fluorescent fatty-acid analog, which we infused along with a fat emulsion through a duodenal cannula. The vessel contractility was simultaneously quantified. We demonstrated for the first time that collecting lymphatic vessels respond to an acute lipid load by reducing pump function. High lipid levels decreased contraction frequency and amplitude. We also showed a strong tonic response through a reduction in the end-diastolic and systolic diameters. We further characterized the changes in flow rate and viscosity and showed that both increase postprandially. In addition, shear-mediated Ca2+ signaling in lymphatic endothelial cells differed when cultured with lipoproteins. Together these results show that the in vivo response could be both shear and lipid mediated and provide the first evidence that high postprandial lipid has an immediate negative effect on lymphatic function even in the acute setting.
Url:
DOI: 10.1152/ajpgi.00318.2015
PubMed: 26968208
PubMed Central: 4888550
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<front><div type="abstract" xml:lang="en"><p>Dietary lipids are transported from the intestine through contractile lymphatics. Chronic lipid loads can adversely affect lymphatic function. However, the acute lymphatic pump response in the mesentery to a postprandial lipid meal has gone unexplored. In this study, we used the rat mesenteric collecting vessel as an in vivo model to quantify the effect of lipoproteins on vessel function. Lipid load was continuously monitored by using the intensity of a fluorescent fatty-acid analog, which we infused along with a fat emulsion through a duodenal cannula. The vessel contractility was simultaneously quantified. We demonstrated for the first time that collecting lymphatic vessels respond to an acute lipid load by reducing pump function. High lipid levels decreased contraction frequency and amplitude. We also showed a strong tonic response through a reduction in the end-diastolic and systolic diameters. We further characterized the changes in flow rate and viscosity and showed that both increase postprandially. In addition, shear-mediated Ca<sup>2+</sup>
signaling in lymphatic endothelial cells differed when cultured with lipoproteins. Together these results show that the in vivo response could be both shear and lipid mediated and provide the first evidence that high postprandial lipid has an immediate negative effect on lymphatic function even in the acute setting.</p>
</div>
</front>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Am J Physiol Gastrointest Liver Physiol</journal-id>
<journal-id journal-id-type="iso-abbrev">Am. J. Physiol. Gastrointest. Liver Physiol</journal-id>
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<article-id pub-id-type="doi">10.1152/ajpgi.00318.2015</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Nutrient Sensing, Nutrition, and Metabolism</subject>
</subj-group>
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<title-group><article-title>Postprandial lymphatic pump function after a high-fat meal: a characterization of contractility, flow, and viscosity</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Kassis</surname>
<given-names>Timothy</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
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<contrib contrib-type="author"><name><surname>Yarlagadda</surname>
<given-names>Sri Charan</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>4</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kohan</surname>
<given-names>Alison B.</given-names>
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<xref ref-type="aff" rid="aff5"><sup>5</sup>
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<contrib contrib-type="author"><name><surname>Tso</surname>
<given-names>Patrick</given-names>
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<xref ref-type="aff" rid="aff5"><sup>5</sup>
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<contrib contrib-type="author"><name><surname>Breedveld</surname>
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<xref ref-type="aff" rid="aff4"><sup>4</sup>
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<contrib contrib-type="author" corresp="yes"><name><surname>Dixon</surname>
<given-names>J. Brandon</given-names>
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<xref ref-type="aff" rid="aff1"><sup>1</sup>
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<xref ref-type="aff" rid="aff3"><sup>3</sup>
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<aff id="aff1"><sup>1</sup>
Parker H. Petit Institute for Bioengineering & Bioscience, Georgia Institute of Technology, Atlanta, Georgia;</aff>
<aff id="aff2"><sup>2</sup>
School of Electrical and Computer Engineering, Georgia Institute of Technology, Atlanta, Georgia;</aff>
<aff id="aff3"><sup>3</sup>
George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, Georgia;</aff>
<aff id="aff4"><sup>4</sup>
School of Chemical & Biomolecular Engineering, Georgia Institute of Technology, Atlanta, Georgia; and</aff>
<aff id="aff5"><sup>5</sup>
Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio</aff>
</contrib-group>
<author-notes><corresp id="cor1">Address for reprint requests and other correspondence: J. B. Dixon, <addr-line>Parker H. Petit Institute for Bioengineering & Bioscience, IBB 2312, 315 Ferst Dr. NW, Atlanta, GA 30332-0405</addr-line>
(e-mail: <email>dixon@gatech.edu</email>
).</corresp>
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<pub-date pub-type="epub"><day>11</day>
<month>3</month>
<year>2016</year>
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<pub-date pub-type="ppub"><day>15</day>
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<pub-date pub-type="pmc-release"><day>15</day>
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0 days and was based on the . </pmc-comment>
<volume>310</volume>
<issue>10</issue>
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<month>9</month>
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<permissions><copyright-statement>Copyright © 2016 the American Physiological Society</copyright-statement>
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<self-uri content-type="pdf" xlink:href="zh301016000776.pdf"></self-uri>
<abstract><p>Dietary lipids are transported from the intestine through contractile lymphatics. Chronic lipid loads can adversely affect lymphatic function. However, the acute lymphatic pump response in the mesentery to a postprandial lipid meal has gone unexplored. In this study, we used the rat mesenteric collecting vessel as an in vivo model to quantify the effect of lipoproteins on vessel function. Lipid load was continuously monitored by using the intensity of a fluorescent fatty-acid analog, which we infused along with a fat emulsion through a duodenal cannula. The vessel contractility was simultaneously quantified. We demonstrated for the first time that collecting lymphatic vessels respond to an acute lipid load by reducing pump function. High lipid levels decreased contraction frequency and amplitude. We also showed a strong tonic response through a reduction in the end-diastolic and systolic diameters. We further characterized the changes in flow rate and viscosity and showed that both increase postprandially. In addition, shear-mediated Ca<sup>2+</sup>
signaling in lymphatic endothelial cells differed when cultured with lipoproteins. Together these results show that the in vivo response could be both shear and lipid mediated and provide the first evidence that high postprandial lipid has an immediate negative effect on lymphatic function even in the acute setting.</p>
</abstract>
<kwd-group><kwd>chylomicron</kwd>
<kwd>lipid absorption</kwd>
<kwd>lymphatic</kwd>
<kwd>pump function</kwd>
<kwd>viscosity</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Géorgie (pays)</li>
<li>États-Unis</li>
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<name sortKey="Dixon, J Brandon" sort="Dixon, J Brandon" uniqKey="Dixon J" first="J. Brandon" last="Dixon">J. Brandon Dixon</name>
<name sortKey="Kassis, Timothy" sort="Kassis, Timothy" uniqKey="Kassis T" first="Timothy" last="Kassis">Timothy Kassis</name>
<name sortKey="Yarlagadda, Sri Charan" sort="Yarlagadda, Sri Charan" uniqKey="Yarlagadda S" first="Sri Charan" last="Yarlagadda">Sri Charan Yarlagadda</name>
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<country name="États-Unis"><region name="Ohio"><name sortKey="Kohan, Alison B" sort="Kohan, Alison B" uniqKey="Kohan A" first="Alison B." last="Kohan">Alison B. Kohan</name>
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