Cloning and sequence analysis of partial genomic DNA coding for HtrA-type serine protease of Wolbachia from human lymphatic filarial parasite, Wuchereria bancrofti
Identifieur interne : 005719 ( Ncbi/Merge ); précédent : 005718; suivant : 005720Cloning and sequence analysis of partial genomic DNA coding for HtrA-type serine protease of Wolbachia from human lymphatic filarial parasite, Wuchereria bancrofti
Auteurs : R. Dhamodharan ; Sl Hoti ; G. Sivapragasam ; Mk DasSource :
- Tropical Parasitology [ 2229-5070 ] ; 2011.
Abstract
Periplasmic serine proteases of HtrA type of
This study was aimed to sequence Wb-HtrA serine protease and analyze its phylogenetic position by comparing with other filarial and non-filarial nematode homologs.
Partial HtrA gene fragment was amplified from DNA isolated from periodic and sub-periodic
Partial orthologue of HtrA-type serine protease from
HtrA-type serine protease from
Url:
DOI: 10.4103/2229-5070.86935
PubMed: 23508470
PubMed Central: 3593480
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PMC:3593480Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cloning and sequence analysis of partial genomic DNA coding for HtrA-type serine protease of <italic>Wolbachia</italic>
from human lymphatic filarial parasite, <italic>Wuchereria bancrofti</italic>
</title>
<author><name sortKey="Dhamodharan, R" sort="Dhamodharan, R" uniqKey="Dhamodharan R" first="R" last="Dhamodharan">R. Dhamodharan</name>
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<author><name sortKey="Hoti, Sl" sort="Hoti, Sl" uniqKey="Hoti S" first="Sl" last="Hoti">Sl Hoti</name>
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<author><name sortKey="Sivapragasam, G" sort="Sivapragasam, G" uniqKey="Sivapragasam G" first="G" last="Sivapragasam">G. Sivapragasam</name>
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<author><name sortKey="Das, Mk" sort="Das, Mk" uniqKey="Das M" first="Mk" last="Das">Mk Das</name>
<affiliation><nlm:aff id="aff2"><italic>National Institute of Malaria Research (ICMR), Ranchi, India</italic>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cloning and sequence analysis of partial genomic DNA coding for HtrA-type serine protease of <italic>Wolbachia</italic>
from human lymphatic filarial parasite, <italic>Wuchereria bancrofti</italic>
</title>
<author><name sortKey="Dhamodharan, R" sort="Dhamodharan, R" uniqKey="Dhamodharan R" first="R" last="Dhamodharan">R. Dhamodharan</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hoti, Sl" sort="Hoti, Sl" uniqKey="Hoti S" first="Sl" last="Hoti">Sl Hoti</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Sivapragasam, G" sort="Sivapragasam, G" uniqKey="Sivapragasam G" first="G" last="Sivapragasam">G. Sivapragasam</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Das, Mk" sort="Das, Mk" uniqKey="Das M" first="Mk" last="Das">Mk Das</name>
<affiliation><nlm:aff id="aff2"><italic>National Institute of Malaria Research (ICMR), Ranchi, India</italic>
</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Tropical Parasitology</title>
<idno type="ISSN">2229-5070</idno>
<idno type="eISSN">2229-7758</idno>
<imprint><date when="2011">2011</date>
</imprint>
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<front><div type="abstract" xml:lang="en"><sec id="st1"><title>Background:</title>
<p>Periplasmic serine proteases of HtrA type of <italic>Wolbachia</italic>
have been shown to play a role in the pathogenesis of filarial disease.</p>
</sec>
<sec id="st2"><title>Aims:</title>
<p>This study was aimed to sequence Wb-HtrA serine protease and analyze its phylogenetic position by comparing with other filarial and non-filarial nematode homologs.</p>
</sec>
<sec id="st3"><title>Materials and Methods:</title>
<p>Partial HtrA gene fragment was amplified from DNA isolated from periodic and sub-periodic <italic>Wuchereria bancrofti</italic>
parasites collected from Pondicherry and Nicobar islands, respectively. The amplicons were sequenced, and sequence homology and phylogenetic relationship with other filarial and non-filarial nematodes were analyzed.</p>
</sec>
<sec id="st4"><title>Results:</title>
<p>Partial orthologue of HtrA-type serine protease from <italic>Wolbachia</italic>
of <italic>W. bancrofti</italic>
was amplified, cloned and sequenced. The deduced amino acid sequence exhibited 87%, 81% and 74% identity with the homologous <italic>Wolbachia</italic>
proteases identified from <italic>Brugia malayi, Onchocerca volvulus</italic>
and <italic>Drosophila melanogaster</italic>
, respectively. The Wb-HtrA has arthologues in several proteobacteria with very high homology and hence is highly conserved not only among <italic>Wolbachia</italic>
of filarial parasites but also across proteobacteria. The phylogenetic tree constructed using Neighbor-Joining method showed two main clusters: cluster-I containing bacteria that dwell in diverse habitats such as soil, fresh and marine waters and plants and cluster-II comprising <italic>Anaplasma</italic>
sp. and <italic>Erlichia</italic>
, and <italic>Wolbachia</italic>
endosymbionts of insects and nematodes, in distinct groups.</p>
</sec>
<sec id="st5"><title>Conclusions:</title>
<p>HtrA-type serine protease from <italic>Wolbachia</italic>
of <italic>W. bancrofti</italic>
is highly conserved among filarial parasites. It will be of interest to know whether filarial <italic>Wolbachia</italic>
HtrA type of serine protease might influence apoptosis and lymphatic epithelium, thereby playing a role in the filarial pathogenesis. Such information will be useful for identifying targets for the development of newer drugs for filariasis treatment, especially for preventing lymphatic pathology.</p>
</sec>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Trop Parasitol</journal-id>
<journal-id journal-id-type="iso-abbrev">Trop Parasitol</journal-id>
<journal-id journal-id-type="publisher-id">TP</journal-id>
<journal-title-group><journal-title>Tropical Parasitology</journal-title>
</journal-title-group>
<issn pub-type="ppub">2229-5070</issn>
<issn pub-type="epub">2229-7758</issn>
<publisher><publisher-name>Medknow Publications & Media Pvt Ltd</publisher-name>
<publisher-loc>India</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">23508470</article-id>
<article-id pub-id-type="pmc">3593480</article-id>
<article-id pub-id-type="publisher-id">TP-1-76</article-id>
<article-id pub-id-type="doi">10.4103/2229-5070.86935</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Cloning and sequence analysis of partial genomic DNA coding for HtrA-type serine protease of <italic>Wolbachia</italic>
from human lymphatic filarial parasite, <italic>Wuchereria bancrofti</italic>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Dhamodharan</surname>
<given-names>R</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Hoti</surname>
<given-names>SL</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
<xref ref-type="corresp" rid="cor1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Sivapragasam</surname>
<given-names>G</given-names>
</name>
<xref ref-type="aff" rid="aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Das</surname>
<given-names>MK</given-names>
</name>
<xref ref-type="aff" rid="aff2">1</xref>
</contrib>
</contrib-group>
<aff id="aff1"><italic>Microbiology and Molecular Biology Division, Vector Control Research Centre (ICMR), Puducherry, India</italic>
</aff>
<aff id="aff2"><label>1</label>
<italic>National Institute of Malaria Research (ICMR), Ranchi, India</italic>
</aff>
<author-notes><corresp id="cor1"><bold>Address for correspondence:</bold>
Dr. SL Hoti, Vector Control Research Centre (ICMR), Medical Complex, Indira Nagar, Puducherry - 605 006, India. E-mail: <email xlink:href="slhoti@yahoo.com">slhoti@yahoo.com</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><season>Jul-Dec</season>
<year>2011</year>
</pub-date>
<volume>1</volume>
<issue>2</issue>
<fpage>76</fpage>
<lpage>82</lpage>
<history><date date-type="received"><day>08</day>
<month>11</month>
<year>2011</year>
</date>
<date date-type="accepted"><day>31</day>
<month>10</month>
<year>2011</year>
</date>
</history>
<permissions><copyright-statement>Copyright: © Tropical Parasitology</copyright-statement>
<copyright-year>2011</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-sa/3.0"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<abstract><sec id="st1"><title>Background:</title>
<p>Periplasmic serine proteases of HtrA type of <italic>Wolbachia</italic>
have been shown to play a role in the pathogenesis of filarial disease.</p>
</sec>
<sec id="st2"><title>Aims:</title>
<p>This study was aimed to sequence Wb-HtrA serine protease and analyze its phylogenetic position by comparing with other filarial and non-filarial nematode homologs.</p>
</sec>
<sec id="st3"><title>Materials and Methods:</title>
<p>Partial HtrA gene fragment was amplified from DNA isolated from periodic and sub-periodic <italic>Wuchereria bancrofti</italic>
parasites collected from Pondicherry and Nicobar islands, respectively. The amplicons were sequenced, and sequence homology and phylogenetic relationship with other filarial and non-filarial nematodes were analyzed.</p>
</sec>
<sec id="st4"><title>Results:</title>
<p>Partial orthologue of HtrA-type serine protease from <italic>Wolbachia</italic>
of <italic>W. bancrofti</italic>
was amplified, cloned and sequenced. The deduced amino acid sequence exhibited 87%, 81% and 74% identity with the homologous <italic>Wolbachia</italic>
proteases identified from <italic>Brugia malayi, Onchocerca volvulus</italic>
and <italic>Drosophila melanogaster</italic>
, respectively. The Wb-HtrA has arthologues in several proteobacteria with very high homology and hence is highly conserved not only among <italic>Wolbachia</italic>
of filarial parasites but also across proteobacteria. The phylogenetic tree constructed using Neighbor-Joining method showed two main clusters: cluster-I containing bacteria that dwell in diverse habitats such as soil, fresh and marine waters and plants and cluster-II comprising <italic>Anaplasma</italic>
sp. and <italic>Erlichia</italic>
, and <italic>Wolbachia</italic>
endosymbionts of insects and nematodes, in distinct groups.</p>
</sec>
<sec id="st5"><title>Conclusions:</title>
<p>HtrA-type serine protease from <italic>Wolbachia</italic>
of <italic>W. bancrofti</italic>
is highly conserved among filarial parasites. It will be of interest to know whether filarial <italic>Wolbachia</italic>
HtrA type of serine protease might influence apoptosis and lymphatic epithelium, thereby playing a role in the filarial pathogenesis. Such information will be useful for identifying targets for the development of newer drugs for filariasis treatment, especially for preventing lymphatic pathology.</p>
</sec>
</abstract>
<kwd-group><title>KEYWORDS</title>
<kwd>HtrA serine protease</kwd>
<kwd>lymphatic filariasis</kwd>
<kwd><italic>Wolbachia</italic>
</kwd>
<kwd><italic>Wuchereria bancrofti</italic>
</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Das, Mk" sort="Das, Mk" uniqKey="Das M" first="Mk" last="Das">Mk Das</name>
<name sortKey="Dhamodharan, R" sort="Dhamodharan, R" uniqKey="Dhamodharan R" first="R" last="Dhamodharan">R. Dhamodharan</name>
<name sortKey="Hoti, Sl" sort="Hoti, Sl" uniqKey="Hoti S" first="Sl" last="Hoti">Sl Hoti</name>
<name sortKey="Sivapragasam, G" sort="Sivapragasam, G" uniqKey="Sivapragasam G" first="G" last="Sivapragasam">G. Sivapragasam</name>
</noCountry>
</tree>
</affiliations>
</record>
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