LymphedemaV1, Ncbi, Merge, bibRecord, 005252

In vitro released antigens in diagnosis and immunomonitoring of filaria and tuberculosis

Identifieur interne : 005252 ( Ncbi/Merge ); précédent : 005251; suivant : 005253

In vitro released antigens in diagnosis and immunomonitoring of filaria and tuberculosis

Auteurs : B. C. Harinath ; Satish Kumar ; M. V. R. Reddy

Source :

Indian Journal of Clinical Biochemistry [ 0970-1915 ] ; 1997.

RBID : PMC:3454302

Abstract

In vitro released antigens by living parasites or bacteria underin vitro maintenance or short term culture showing specific humoral immune response have been explored in development of immunodiagnostics for infectious diseases such as filariasis and tuberculosis in our laboratory. ELISA usingB. malayi mf ES antigen has been explored for detecting IgG antibody by Indirect ELISA and antigen by Inhibition ELISA and in immunomonitoring of carriers as well as clinical filarial cases. A ten year follow up of mf carriers with DEC therapy showed disapperance of antigen and antibody followed by reappearance in few cases in an endemic area. None of the cases followed developed clinical symptoms suggesting the need for long term monitoring and treatment of microfilaraemic carriers. Further immunomonitoring was found to be useful in confirming filaria aetiology in the absence of microfilaremia and determining appropriate period of treatment of acute, early clinical and occult filarial infections for clinical relief and cure.

Indirect Stick Penicillinase ELISA system using Mtb EST-6 antigen for detecting tuberculous IgG antibody and a Sandwich Penicillinase ELISA system using affinity purified antibody for detecting circulating antigen were explored in tuberculosis. A combination of both the assay systems with a sensitivity of 70% and specificity of 98% was found to be promising in the precise diagnosis of pulmonary tuberculosis. Further antigen detection was found to be useful in bone and joint tuberculosis.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3454302

DOI: 10.1007/BF02873045
PubMed: 23100885
PubMed Central: 3454302

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PMC:3454302

Le document en format XML

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 released antigens in diagnosis and immunomonitoring of filaria and tuberculosis</title>
<author><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B. C." last="Harinath">B. C. Harinath</name>
<affiliation><nlm:aff id="Aff1">Department of Biochemistry and Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences SEVAGRAM, 442 102 (Wardha), Maharashtra,</nlm:aff>
<wicri:noCountry code="subfield">Maharashtra</wicri:noCountry>
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<author><name sortKey="Kumar, Satish" sort="Kumar, Satish" uniqKey="Kumar S" first="Satish" last="Kumar">Satish Kumar</name>
<affiliation><nlm:aff id="Aff1">Department of Biochemistry and Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences SEVAGRAM, 442 102 (Wardha), Maharashtra,</nlm:aff>
<wicri:noCountry code="subfield">Maharashtra</wicri:noCountry>
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<author><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M. V. R." last="Reddy">M. V. R. Reddy</name>
<affiliation><nlm:aff id="Aff1">Department of Biochemistry and Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences SEVAGRAM, 442 102 (Wardha), Maharashtra,</nlm:aff>
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 released antigens in diagnosis and immunomonitoring of filaria and tuberculosis</title>
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<author><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M. V. R." last="Reddy">M. V. R. Reddy</name>
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<series><title level="j">Indian Journal of Clinical Biochemistry</title>
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<front><div type="abstract" xml:lang="en"><p><italic>In vitro</italic>
 released antigens by living parasites or bacteria under<italic>in vitro</italic>
 maintenance or short term culture showing specific humoral immune response have been explored in development of immunodiagnostics for infectious diseases such as filariasis and tuberculosis in our laboratory. ELISA using<italic>B. malayi</italic>
 mf ES antigen has been explored for detecting IgG antibody by Indirect ELISA and antigen by Inhibition ELISA and in immunomonitoring of carriers as well as clinical filarial cases. A ten year follow up of mf carriers with DEC therapy showed disapperance of antigen and antibody followed by reappearance in few cases in an endemic area. None of the cases followed developed clinical symptoms suggesting the need for long term monitoring and treatment of microfilaraemic carriers. Further immunomonitoring was found to be useful in confirming filaria aetiology in the absence of microfilaremia and determining appropriate period of treatment of acute, early clinical and occult filarial infections for clinical relief and cure.</p>
<p>Indirect Stick Penicillinase ELISA system using Mtb EST-6 antigen for detecting tuberculous IgG antibody and a Sandwich Penicillinase ELISA system using affinity purified antibody for detecting circulating antigen were explored in tuberculosis. A combination of both the assay systems with a sensitivity of 70% and specificity of 98% was found to be promising in the precise diagnosis of pulmonary tuberculosis. Further antigen detection was found to be useful in bone and joint tuberculosis.</p>
</div>
</front>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <front><journal-meta><journal-id journal-id-type="nlm-ta">Indian J Clin Biochem</journal-id>
<journal-id journal-id-type="iso-abbrev">Indian J Clin Biochem</journal-id>
<journal-title-group><journal-title>Indian Journal of Clinical Biochemistry</journal-title>
</journal-title-group>
<issn pub-type="ppub">0970-1915</issn>
<issn pub-type="epub">0974-0422</issn>
<publisher><publisher-name>Springer India</publisher-name>
<publisher-loc>New Delhi</publisher-loc>
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<article-id pub-id-type="pmc">3454302</article-id>
<article-id pub-id-type="publisher-id">BF02873045</article-id>
<article-id pub-id-type="doi">10.1007/BF02873045</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Filariasis</subject>
</subj-group>
</article-categories>
<title-group><article-title><italic>In vitro</italic>
 released antigens in diagnosis and immunomonitoring of filaria and tuberculosis</article-title>
</title-group>
<contrib-group><contrib contrib-type="author" corresp="yes"><name><surname>Harinath</surname>
<given-names>B. C.</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kumar</surname>
<given-names>Satish</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Reddy</surname>
<given-names>M. V. R.</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
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<aff id="Aff1">Department of Biochemistry and Jamnalal Bajaj Tropical Disease Research Centre, Mahatma Gandhi Institute of Medical Sciences SEVAGRAM, 442 102 (Wardha), Maharashtra,</aff>
</contrib-group>
<pub-date pub-type="ppub"><month>12</month>
<year>1997</year>
</pub-date>
<volume>12</volume>
<issue>Suppl 1</issue>
<fpage>8</fpage>
<lpage>12</lpage>
<permissions><copyright-statement>© Association of Clinical Biochemists of India 1997</copyright-statement>
</permissions>
<abstract id="Abs1"><p><italic>In vitro</italic>
 released antigens by living parasites or bacteria under<italic>in vitro</italic>
 maintenance or short term culture showing specific humoral immune response have been explored in development of immunodiagnostics for infectious diseases such as filariasis and tuberculosis in our laboratory. ELISA using<italic>B. malayi</italic>
 mf ES antigen has been explored for detecting IgG antibody by Indirect ELISA and antigen by Inhibition ELISA and in immunomonitoring of carriers as well as clinical filarial cases. A ten year follow up of mf carriers with DEC therapy showed disapperance of antigen and antibody followed by reappearance in few cases in an endemic area. None of the cases followed developed clinical symptoms suggesting the need for long term monitoring and treatment of microfilaraemic carriers. Further immunomonitoring was found to be useful in confirming filaria aetiology in the absence of microfilaremia and determining appropriate period of treatment of acute, early clinical and occult filarial infections for clinical relief and cure.</p>
<p>Indirect Stick Penicillinase ELISA system using Mtb EST-6 antigen for detecting tuberculous IgG antibody and a Sandwich Penicillinase ELISA system using affinity purified antibody for detecting circulating antigen were explored in tuberculosis. A combination of both the assay systems with a sensitivity of 70% and specificity of 98% was found to be promising in the precise diagnosis of pulmonary tuberculosis. Further antigen detection was found to be useful in bone and joint tuberculosis.</p>
</abstract>
<kwd-group xml:lang="en"><title>Key Words</title>
<kwd>ES antigens</kwd>
<kwd>ELISA</kwd>
<kwd>Immunodiagnostics</kwd>
<kwd>Immunomonitoring filariasis</kwd>
<kwd>tuberculosis</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© Association of Clinical Biochemists of India 1997</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B. C." last="Harinath">B. C. Harinath</name>
<name sortKey="Kumar, Satish" sort="Kumar, Satish" uniqKey="Kumar S" first="Satish" last="Kumar">Satish Kumar</name>
<name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M. V. R." last="Reddy">M. V. R. Reddy</name>
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In vitro released antigens in diagnosis and immunomonitoring of filaria and tuberculosis

In vitro released antigens in diagnosis and immunomonitoring of filaria and tuberculosis

Source :

Abstract

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