LymphedemaV1, Ncbi, Merge, bibRecord, 004B62

Cloning and characterization of high mobility group box protein 1 (HMGB1) of Wuchereria bancrofti and Brugia malayi

Identifieur interne : 004B62 ( Ncbi/Merge ); précédent : 004B61; suivant : 004B63

Cloning and characterization of high mobility group box protein 1 (HMGB1) of Wuchereria bancrofti and Brugia malayi

Auteurs : Sivasakthivel Thirugnanam ; Gnanasekar Munirathinam ; Anandharaman Veerapathran ; Gajalakshmi Dakshinamoorthy ; Maryada V. Reddy ; Kalyanasundaram Ramaswamy

Source :

Parasitology research [ 0932-0113 ] ; 2012.

RBID : PMC:3480192

Abstract

A human homologue of high mobility group box 1 (HMGB1) protein was cloned and characterized from the human filarial parasites Wuchereria bancrofti and Brugia malayi. Sequence analysis showed that W. bancrofti HMGB1 (WbHMGB1) and B. malayi HMGB1 (BmHMGB1) proteins share 99 % sequence identity. Filarial HMGB1 showed typical architectural sequence characteristics of HMGB family of proteins and consisted of only a single HMG box domain that had significant sequence similarity to the pro-inflammatory B box domain of human HMGB1. When incubated with mouse peritoneal macrophages and human promyelocytic leukemia cells, rBmHMGB1 induced secretion of significant levels of pro-inflammatory cytokines such as TNF-α, GM-CSF, and IL-6. Functional analysis also showed that the filarial HMGB1 proteins can bind to supercoiled DNA similar to other HMG family of proteins. BmHMGB1 protein is expressed in the adult and microfilarial stages of the parasite and is found in the excretory secretions of the live parasites. These findings suggest that filarial HMGB1 may have a significant role in lymphatic pathology associated with lymphatic filariasis.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480192

DOI: 10.1007/s00436-012-2878-x
PubMed: 22402610
PubMed Central: 3480192

Links toward previous steps (curation, corpus...)

to stream Pmc, to step Corpus: 003835
to stream Pmc, to step Curation: 003834
to stream Pmc, to step Checkpoint: 002B04

Links to Exploration step

PMC:3480192

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cloning and characterization of high mobility group box protein 1 (HMGB1) of <italic>Wuchereria bancrofti</italic>
 and <italic>Brugia malayi</italic>
</title>
<author><name sortKey="Thirugnanam, Sivasakthivel" sort="Thirugnanam, Sivasakthivel" uniqKey="Thirugnanam S" first="Sivasakthivel" last="Thirugnanam">Sivasakthivel Thirugnanam</name>
</author>
<author><name sortKey="Munirathinam, Gnanasekar" sort="Munirathinam, Gnanasekar" uniqKey="Munirathinam G" first="Gnanasekar" last="Munirathinam">Gnanasekar Munirathinam</name>
</author>
<author><name sortKey="Veerapathran, Anandharaman" sort="Veerapathran, Anandharaman" uniqKey="Veerapathran A" first="Anandharaman" last="Veerapathran">Anandharaman Veerapathran</name>
</author>
<author><name sortKey="Dakshinamoorthy, Gajalakshmi" sort="Dakshinamoorthy, Gajalakshmi" uniqKey="Dakshinamoorthy G" first="Gajalakshmi" last="Dakshinamoorthy">Gajalakshmi Dakshinamoorthy</name>
</author>
<author><name sortKey="Reddy, Maryada V" sort="Reddy, Maryada V" uniqKey="Reddy M" first="Maryada V." last="Reddy">Maryada V. Reddy</name>
</author>
<author><name sortKey="Ramaswamy, Kalyanasundaram" sort="Ramaswamy, Kalyanasundaram" uniqKey="Ramaswamy K" first="Kalyanasundaram" last="Ramaswamy">Kalyanasundaram Ramaswamy</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">22402610</idno>
<idno type="pmc">3480192</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3480192</idno>
<idno type="RBID">PMC:3480192</idno>
<idno type="doi">10.1007/s00436-012-2878-x</idno>
<date when="2012">2012</date>
<idno type="wicri:Area/Pmc/Corpus">003835</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003835</idno>
<idno type="wicri:Area/Pmc/Curation">003834</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003834</idno>
<idno type="wicri:Area/Pmc/Checkpoint">002B04</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002B04</idno>
<idno type="wicri:Area/Ncbi/Merge">004B62</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cloning and characterization of high mobility group box protein 1 (HMGB1) of <italic>Wuchereria bancrofti</italic>
 and <italic>Brugia malayi</italic>
</title>
<author><name sortKey="Thirugnanam, Sivasakthivel" sort="Thirugnanam, Sivasakthivel" uniqKey="Thirugnanam S" first="Sivasakthivel" last="Thirugnanam">Sivasakthivel Thirugnanam</name>
</author>
<author><name sortKey="Munirathinam, Gnanasekar" sort="Munirathinam, Gnanasekar" uniqKey="Munirathinam G" first="Gnanasekar" last="Munirathinam">Gnanasekar Munirathinam</name>
</author>
<author><name sortKey="Veerapathran, Anandharaman" sort="Veerapathran, Anandharaman" uniqKey="Veerapathran A" first="Anandharaman" last="Veerapathran">Anandharaman Veerapathran</name>
</author>
<author><name sortKey="Dakshinamoorthy, Gajalakshmi" sort="Dakshinamoorthy, Gajalakshmi" uniqKey="Dakshinamoorthy G" first="Gajalakshmi" last="Dakshinamoorthy">Gajalakshmi Dakshinamoorthy</name>
</author>
<author><name sortKey="Reddy, Maryada V" sort="Reddy, Maryada V" uniqKey="Reddy M" first="Maryada V." last="Reddy">Maryada V. Reddy</name>
</author>
<author><name sortKey="Ramaswamy, Kalyanasundaram" sort="Ramaswamy, Kalyanasundaram" uniqKey="Ramaswamy K" first="Kalyanasundaram" last="Ramaswamy">Kalyanasundaram Ramaswamy</name>
</author>
</analytic>
<series><title level="j">Parasitology research</title>
<idno type="ISSN">0932-0113</idno>
<idno type="eISSN">1432-1955</idno>
<imprint><date when="2012">2012</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">A human homologue of high mobility group box 1 (HMGB1) protein was cloned and characterized from the human filarial parasites <italic>Wuchereria bancrofti</italic>
 and <italic>Brugia malayi</italic>
. Sequence analysis showed that <italic>W. bancrofti</italic>
 HMGB1 (WbHMGB1) and <italic>B. malayi</italic>
 HMGB1 (BmHMGB1) proteins share 99 % sequence identity. Filarial HMGB1 showed typical architectural sequence characteristics of HMGB family of proteins and consisted of only a single HMG box domain that had significant sequence similarity to the pro-inflammatory B box domain of human HMGB1. When incubated with mouse peritoneal macrophages and human promyelocytic leukemia cells, rBmHMGB1 induced secretion of significant levels of pro-inflammatory cytokines such as TNF-α, GM-CSF, and IL-6. Functional analysis also showed that the filarial HMGB1 proteins can bind to supercoiled DNA similar to other HMG family of proteins. BmHMGB1 protein is expressed in the adult and microfilarial stages of the parasite and is found in the excretory secretions of the live parasites. These findings suggest that filarial HMGB1 may have a significant role in lymphatic pathology associated with lymphatic filariasis.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <pmc-dir>properties manuscript</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-journal-id">8703571</journal-id>
<journal-id journal-id-type="pubmed-jr-id">6620</journal-id>
<journal-id journal-id-type="nlm-ta">Parasitol Res</journal-id>
<journal-id journal-id-type="iso-abbrev">Parasitol. Res.</journal-id>
<journal-title-group><journal-title>Parasitology research</journal-title>
</journal-title-group>
<issn pub-type="ppub">0932-0113</issn>
<issn pub-type="epub">1432-1955</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">22402610</article-id>
<article-id pub-id-type="pmc">3480192</article-id>
<article-id pub-id-type="doi">10.1007/s00436-012-2878-x</article-id>
<article-id pub-id-type="manuscript">NIHMS410194</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Cloning and characterization of high mobility group box protein 1 (HMGB1) of <italic>Wuchereria bancrofti</italic>
 and <italic>Brugia malayi</italic>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Thirugnanam</surname>
<given-names>Sivasakthivel</given-names>
</name>
<aff id="A1">Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Munirathinam</surname>
<given-names>Gnanasekar</given-names>
</name>
<aff id="A2">Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Veerapathran</surname>
<given-names>Anandharaman</given-names>
</name>
<aff id="A3">Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Dakshinamoorthy</surname>
<given-names>Gajalakshmi</given-names>
</name>
<aff id="A4">Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Reddy</surname>
<given-names>Maryada V.</given-names>
</name>
<aff id="A5">Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Wardha, India</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Ramaswamy</surname>
<given-names>Kalyanasundaram</given-names>
</name>
<email>ramswamy@uic.edu</email>
<aff id="A6">Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA</aff>
</contrib>
</contrib-group>
<author-notes><fn id="FN1" fn-type="equal"><p>Sivasakthivel Thirugnanam and Gnanasekar Munirathinam contributed equally to the study.</p>
</fn>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>26</day>
<month>9</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="epub"><day>09</day>
<month>3</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="ppub"><month>8</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>24</day>
<month>10</month>
<year>2012</year>
</pub-date>
<volume>111</volume>
<issue>2</issue>
<fpage>619</fpage>
<lpage>627</lpage>
<permissions><copyright-statement>© Springer-Verlag 2012</copyright-statement>
<copyright-year>2012</copyright-year>
</permissions>
<abstract><p id="P1">A human homologue of high mobility group box 1 (HMGB1) protein was cloned and characterized from the human filarial parasites <italic>Wuchereria bancrofti</italic>
 and <italic>Brugia malayi</italic>
. Sequence analysis showed that <italic>W. bancrofti</italic>
 HMGB1 (WbHMGB1) and <italic>B. malayi</italic>
 HMGB1 (BmHMGB1) proteins share 99 % sequence identity. Filarial HMGB1 showed typical architectural sequence characteristics of HMGB family of proteins and consisted of only a single HMG box domain that had significant sequence similarity to the pro-inflammatory B box domain of human HMGB1. When incubated with mouse peritoneal macrophages and human promyelocytic leukemia cells, rBmHMGB1 induced secretion of significant levels of pro-inflammatory cytokines such as TNF-α, GM-CSF, and IL-6. Functional analysis also showed that the filarial HMGB1 proteins can bind to supercoiled DNA similar to other HMG family of proteins. BmHMGB1 protein is expressed in the adult and microfilarial stages of the parasite and is found in the excretory secretions of the live parasites. These findings suggest that filarial HMGB1 may have a significant role in lymphatic pathology associated with lymphatic filariasis.</p>
</abstract>
<funding-group><award-group><funding-source country="United States">National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID</funding-source>
<award-id>R56 AI064745 || AI</award-id>
</award-group>
<award-group><funding-source country="United States">National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID</funding-source>
<award-id>R01 AI064745 || AI</award-id>
</award-group>
</funding-group>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Dakshinamoorthy, Gajalakshmi" sort="Dakshinamoorthy, Gajalakshmi" uniqKey="Dakshinamoorthy G" first="Gajalakshmi" last="Dakshinamoorthy">Gajalakshmi Dakshinamoorthy</name>
<name sortKey="Munirathinam, Gnanasekar" sort="Munirathinam, Gnanasekar" uniqKey="Munirathinam G" first="Gnanasekar" last="Munirathinam">Gnanasekar Munirathinam</name>
<name sortKey="Ramaswamy, Kalyanasundaram" sort="Ramaswamy, Kalyanasundaram" uniqKey="Ramaswamy K" first="Kalyanasundaram" last="Ramaswamy">Kalyanasundaram Ramaswamy</name>
<name sortKey="Reddy, Maryada V" sort="Reddy, Maryada V" uniqKey="Reddy M" first="Maryada V." last="Reddy">Maryada V. Reddy</name>
<name sortKey="Thirugnanam, Sivasakthivel" sort="Thirugnanam, Sivasakthivel" uniqKey="Thirugnanam S" first="Sivasakthivel" last="Thirugnanam">Sivasakthivel Thirugnanam</name>
<name sortKey="Veerapathran, Anandharaman" sort="Veerapathran, Anandharaman" uniqKey="Veerapathran A" first="Anandharaman" last="Veerapathran">Anandharaman Veerapathran</name>
</noCountry>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Ncbi/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004B62 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 004B62 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Sante
   |area=    LymphedemaV1
   |flux=    Ncbi
   |étape=   Merge
   |type=    RBID
   |clé=     PMC:3480192
   |texte=   Cloning and characterization of high mobility group box protein 1 (HMGB1) of Wuchereria bancrofti and Brugia malayi
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i   -Sk "pubmed:22402610" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd   \
       | NlmPubMed2Wicri -a LymphedemaV1

This area was generated with Dilib version V0.6.31.
Data generation: Sat Nov 4 17:40:35 2017. Site generation: Tue Feb 13 16:42:16 2024

	Serveur d'exploration sur le lymphœdème
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le lymphœdème

Cloning and characterization of high mobility group box protein 1 (HMGB1) of Wuchereria bancrofti and Brugia malayi

Cloning and characterization of high mobility group box protein 1 (HMGB1) of Wuchereria bancrofti and Brugia malayi

Source :

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki