Cloning and characterization of high mobility group box protein 1 (HMGB1) of Wuchereria bancrofti and Brugia malayi
Identifieur interne : 004B62 ( Ncbi/Merge ); précédent : 004B61; suivant : 004B63Cloning and characterization of high mobility group box protein 1 (HMGB1) of Wuchereria bancrofti and Brugia malayi
Auteurs : Sivasakthivel Thirugnanam ; Gnanasekar Munirathinam ; Anandharaman Veerapathran ; Gajalakshmi Dakshinamoorthy ; Maryada V. Reddy ; Kalyanasundaram RamaswamySource :
- Parasitology research [ 0932-0113 ] ; 2012.
Abstract
A human homologue of high mobility group box 1 (HMGB1) protein was cloned and characterized from the human filarial parasites
Url:
DOI: 10.1007/s00436-012-2878-x
PubMed: 22402610
PubMed Central: 3480192
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and <italic>Brugia malayi</italic>
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<author><name sortKey="Thirugnanam, Sivasakthivel" sort="Thirugnanam, Sivasakthivel" uniqKey="Thirugnanam S" first="Sivasakthivel" last="Thirugnanam">Sivasakthivel Thirugnanam</name>
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<author><name sortKey="Munirathinam, Gnanasekar" sort="Munirathinam, Gnanasekar" uniqKey="Munirathinam G" first="Gnanasekar" last="Munirathinam">Gnanasekar Munirathinam</name>
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<author><name sortKey="Veerapathran, Anandharaman" sort="Veerapathran, Anandharaman" uniqKey="Veerapathran A" first="Anandharaman" last="Veerapathran">Anandharaman Veerapathran</name>
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<author><name sortKey="Dakshinamoorthy, Gajalakshmi" sort="Dakshinamoorthy, Gajalakshmi" uniqKey="Dakshinamoorthy G" first="Gajalakshmi" last="Dakshinamoorthy">Gajalakshmi Dakshinamoorthy</name>
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<author><name sortKey="Reddy, Maryada V" sort="Reddy, Maryada V" uniqKey="Reddy M" first="Maryada V." last="Reddy">Maryada V. Reddy</name>
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<author><name sortKey="Ramaswamy, Kalyanasundaram" sort="Ramaswamy, Kalyanasundaram" uniqKey="Ramaswamy K" first="Kalyanasundaram" last="Ramaswamy">Kalyanasundaram Ramaswamy</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cloning and characterization of high mobility group box protein 1 (HMGB1) of <italic>Wuchereria bancrofti</italic>
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</title>
<author><name sortKey="Thirugnanam, Sivasakthivel" sort="Thirugnanam, Sivasakthivel" uniqKey="Thirugnanam S" first="Sivasakthivel" last="Thirugnanam">Sivasakthivel Thirugnanam</name>
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<author><name sortKey="Munirathinam, Gnanasekar" sort="Munirathinam, Gnanasekar" uniqKey="Munirathinam G" first="Gnanasekar" last="Munirathinam">Gnanasekar Munirathinam</name>
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<author><name sortKey="Veerapathran, Anandharaman" sort="Veerapathran, Anandharaman" uniqKey="Veerapathran A" first="Anandharaman" last="Veerapathran">Anandharaman Veerapathran</name>
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<author><name sortKey="Dakshinamoorthy, Gajalakshmi" sort="Dakshinamoorthy, Gajalakshmi" uniqKey="Dakshinamoorthy G" first="Gajalakshmi" last="Dakshinamoorthy">Gajalakshmi Dakshinamoorthy</name>
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<author><name sortKey="Reddy, Maryada V" sort="Reddy, Maryada V" uniqKey="Reddy M" first="Maryada V." last="Reddy">Maryada V. Reddy</name>
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<author><name sortKey="Ramaswamy, Kalyanasundaram" sort="Ramaswamy, Kalyanasundaram" uniqKey="Ramaswamy K" first="Kalyanasundaram" last="Ramaswamy">Kalyanasundaram Ramaswamy</name>
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<series><title level="j">Parasitology research</title>
<idno type="ISSN">0932-0113</idno>
<idno type="eISSN">1432-1955</idno>
<imprint><date when="2012">2012</date>
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<front><div type="abstract" xml:lang="en"><p id="P1">A human homologue of high mobility group box 1 (HMGB1) protein was cloned and characterized from the human filarial parasites <italic>Wuchereria bancrofti</italic>
and <italic>Brugia malayi</italic>
. Sequence analysis showed that <italic>W. bancrofti</italic>
HMGB1 (WbHMGB1) and <italic>B. malayi</italic>
HMGB1 (BmHMGB1) proteins share 99 % sequence identity. Filarial HMGB1 showed typical architectural sequence characteristics of HMGB family of proteins and consisted of only a single HMG box domain that had significant sequence similarity to the pro-inflammatory B box domain of human HMGB1. When incubated with mouse peritoneal macrophages and human promyelocytic leukemia cells, rBmHMGB1 induced secretion of significant levels of pro-inflammatory cytokines such as TNF-α, GM-CSF, and IL-6. Functional analysis also showed that the filarial HMGB1 proteins can bind to supercoiled DNA similar to other HMG family of proteins. BmHMGB1 protein is expressed in the adult and microfilarial stages of the parasite and is found in the excretory secretions of the live parasites. These findings suggest that filarial HMGB1 may have a significant role in lymphatic pathology associated with lymphatic filariasis.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">8703571</journal-id>
<journal-id journal-id-type="pubmed-jr-id">6620</journal-id>
<journal-id journal-id-type="nlm-ta">Parasitol Res</journal-id>
<journal-id journal-id-type="iso-abbrev">Parasitol. Res.</journal-id>
<journal-title-group><journal-title>Parasitology research</journal-title>
</journal-title-group>
<issn pub-type="ppub">0932-0113</issn>
<issn pub-type="epub">1432-1955</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">22402610</article-id>
<article-id pub-id-type="pmc">3480192</article-id>
<article-id pub-id-type="doi">10.1007/s00436-012-2878-x</article-id>
<article-id pub-id-type="manuscript">NIHMS410194</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Cloning and characterization of high mobility group box protein 1 (HMGB1) of <italic>Wuchereria bancrofti</italic>
and <italic>Brugia malayi</italic>
</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Thirugnanam</surname>
<given-names>Sivasakthivel</given-names>
</name>
<aff id="A1">Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Munirathinam</surname>
<given-names>Gnanasekar</given-names>
</name>
<aff id="A2">Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Veerapathran</surname>
<given-names>Anandharaman</given-names>
</name>
<aff id="A3">Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Dakshinamoorthy</surname>
<given-names>Gajalakshmi</given-names>
</name>
<aff id="A4">Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Reddy</surname>
<given-names>Maryada V.</given-names>
</name>
<aff id="A5">Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Wardha, India</aff>
</contrib>
<contrib contrib-type="author"><name><surname>Ramaswamy</surname>
<given-names>Kalyanasundaram</given-names>
</name>
<email>ramswamy@uic.edu</email>
<aff id="A6">Department of Biomedical Sciences, College of Medicine, University of Illinois, Rockford, IL 61107, USA</aff>
</contrib>
</contrib-group>
<author-notes><fn id="FN1" fn-type="equal"><p>Sivasakthivel Thirugnanam and Gnanasekar Munirathinam contributed equally to the study.</p>
</fn>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>26</day>
<month>9</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="epub"><day>09</day>
<month>3</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="ppub"><month>8</month>
<year>2012</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>24</day>
<month>10</month>
<year>2012</year>
</pub-date>
<volume>111</volume>
<issue>2</issue>
<fpage>619</fpage>
<lpage>627</lpage>
<permissions><copyright-statement>© Springer-Verlag 2012</copyright-statement>
<copyright-year>2012</copyright-year>
</permissions>
<abstract><p id="P1">A human homologue of high mobility group box 1 (HMGB1) protein was cloned and characterized from the human filarial parasites <italic>Wuchereria bancrofti</italic>
and <italic>Brugia malayi</italic>
. Sequence analysis showed that <italic>W. bancrofti</italic>
HMGB1 (WbHMGB1) and <italic>B. malayi</italic>
HMGB1 (BmHMGB1) proteins share 99 % sequence identity. Filarial HMGB1 showed typical architectural sequence characteristics of HMGB family of proteins and consisted of only a single HMG box domain that had significant sequence similarity to the pro-inflammatory B box domain of human HMGB1. When incubated with mouse peritoneal macrophages and human promyelocytic leukemia cells, rBmHMGB1 induced secretion of significant levels of pro-inflammatory cytokines such as TNF-α, GM-CSF, and IL-6. Functional analysis also showed that the filarial HMGB1 proteins can bind to supercoiled DNA similar to other HMG family of proteins. BmHMGB1 protein is expressed in the adult and microfilarial stages of the parasite and is found in the excretory secretions of the live parasites. These findings suggest that filarial HMGB1 may have a significant role in lymphatic pathology associated with lymphatic filariasis.</p>
</abstract>
<funding-group><award-group><funding-source country="United States">National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID</funding-source>
<award-id>R56 AI064745 || AI</award-id>
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<award-group><funding-source country="United States">National Institute of Allergy and Infectious Diseases Extramural Activities : NIAID</funding-source>
<award-id>R01 AI064745 || AI</award-id>
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</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Dakshinamoorthy, Gajalakshmi" sort="Dakshinamoorthy, Gajalakshmi" uniqKey="Dakshinamoorthy G" first="Gajalakshmi" last="Dakshinamoorthy">Gajalakshmi Dakshinamoorthy</name>
<name sortKey="Munirathinam, Gnanasekar" sort="Munirathinam, Gnanasekar" uniqKey="Munirathinam G" first="Gnanasekar" last="Munirathinam">Gnanasekar Munirathinam</name>
<name sortKey="Ramaswamy, Kalyanasundaram" sort="Ramaswamy, Kalyanasundaram" uniqKey="Ramaswamy K" first="Kalyanasundaram" last="Ramaswamy">Kalyanasundaram Ramaswamy</name>
<name sortKey="Reddy, Maryada V" sort="Reddy, Maryada V" uniqKey="Reddy M" first="Maryada V." last="Reddy">Maryada V. Reddy</name>
<name sortKey="Thirugnanam, Sivasakthivel" sort="Thirugnanam, Sivasakthivel" uniqKey="Thirugnanam S" first="Sivasakthivel" last="Thirugnanam">Sivasakthivel Thirugnanam</name>
<name sortKey="Veerapathran, Anandharaman" sort="Veerapathran, Anandharaman" uniqKey="Veerapathran A" first="Anandharaman" last="Veerapathran">Anandharaman Veerapathran</name>
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