Expression of Podoplanin and Classical Cadherins in Salivary Gland Epithelial Cells of Klotho-Deficient Mice
Identifieur interne : 004A23 ( Ncbi/Merge ); précédent : 004A22; suivant : 004A24Expression of Podoplanin and Classical Cadherins in Salivary Gland Epithelial Cells of Klotho-Deficient Mice
Auteurs : Ikuko Amano [Japon] ; Yuri Imaizumi [Japon] ; Chiaki Kaji [Japon] ; Hiroshi Kojima [Japon] ; Yoshihiko Sawa [Japon]Source :
- Acta Histochemica et Cytochemica [ 0044-5991 ] ; 2011.
Abstract
We have recently shown that salivary gland myoepithelial cells express podoplanin. Podoplanin indirectly binds the actin filament network which links classical cadherins. The study here is aimed to investigate the expression of podoplanin and cadherins on salivary gland myoepithelial cells and the changes in the aging cells using klotho-deficient (kl/kl) mice. The submandibular glands of kl/kl mouse lack granular ducts which express klotho in wild type mice, suggesting that klotho may be a gene responsible for granular duct development. Although aging resulted in growth suppression of myoepithelial cells because of the sparse distribution of the cells in kl/kl mouse salivary glands, the expression of podoplanin and E-cadherin was shown in aging myoepithelial cells. It is thought that podoplanin participates in the actin-E-cadherin networks which are maintained in aging myoepithelial cells. It was also shown that granular ducts were filled with P-cadherin, and that the P-cadherin amount was larger in the wild type mouse submandibular glands than in the sublingual and parotid glands of wild type mouse, and in the submandibular glands of kl/kl mouse. These findings suggest that the granular duct is an organ secreting soluble P-cadherin into the saliva.
Url:
DOI: 10.1267/ahc.11037
PubMed: 22282587
PubMed Central: 3263859
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College</wicri:noRegion></affiliation></author><author><name sortKey="Imaizumi, Yuri" sort="Imaizumi, Yuri" uniqKey="Imaizumi Y" first="Yuri" last="Imaizumi">Yuri Imaizumi</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Oral Growth & Development, Fukuoka Dental College, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Oral Growth & Development, Fukuoka Dental College</wicri:regionArea><wicri:noRegion>Fukuoka Dental College</wicri:noRegion></affiliation></author><author><name sortKey="Kaji, Chiaki" sort="Kaji, Chiaki" uniqKey="Kaji C" first="Chiaki" last="Kaji">Chiaki Kaji</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Oral Growth & Development, Fukuoka Dental College, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Oral Growth & Development, Fukuoka Dental College</wicri:regionArea><wicri:noRegion>Fukuoka Dental College</wicri:noRegion></affiliation></author><author><name sortKey="Kojima, Hiroshi" sort="Kojima, Hiroshi" uniqKey="Kojima H" first="Hiroshi" last="Kojima">Hiroshi Kojima</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Oral Growth & Development, Fukuoka Dental College, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Oral Growth & Development, Fukuoka Dental College</wicri:regionArea><wicri:noRegion>Fukuoka Dental College</wicri:noRegion></affiliation></author><author><name sortKey="Sawa, Yoshihiko" sort="Sawa, Yoshihiko" uniqKey="Sawa Y" first="Yoshihiko" last="Sawa">Yoshihiko Sawa</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Morphological Biology, Fukuoka Dental College, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Morphological Biology, Fukuoka Dental College</wicri:regionArea><wicri:noRegion>Fukuoka Dental College</wicri:noRegion></affiliation></author></analytic><series><title level="j">Acta Histochemica et Cytochemica</title><idno type="ISSN">0044-5991</idno><idno type="eISSN">1347-5800</idno><imprint><date when="2011">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We have recently shown that salivary gland myoepithelial cells express podoplanin. Podoplanin indirectly binds the actin filament network which links classical cadherins. The study here is aimed to investigate the expression of podoplanin and cadherins on salivary gland myoepithelial cells and the changes in the aging cells using klotho-deficient (kl/kl) mice. The submandibular glands of kl/kl mouse lack granular ducts which express klotho in wild type mice, suggesting that klotho may be a gene responsible for granular duct development. Although aging resulted in growth suppression of myoepithelial cells because of the sparse distribution of the cells in kl/kl mouse salivary glands, the expression of podoplanin and E-cadherin was shown in aging myoepithelial cells. It is thought that podoplanin participates in the actin-E-cadherin networks which are maintained in aging myoepithelial cells. It was also shown that granular ducts were filled with P-cadherin, and that the P-cadherin amount was larger in the wild type mouse submandibular glands than in the sublingual and parotid glands of wild type mouse, and in the submandibular glands of kl/kl mouse. These findings suggest that the granular duct is an organ secreting soluble P-cadherin into the saliva.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Aberle, H" uniqKey="Aberle H">H. Aberle</name></author><author><name sortKey="Schwartz, H" uniqKey="Schwartz H">H. Schwartz</name></author><author><name sortKey="Kemler, R" uniqKey="Kemler R">R. Kemler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Amano, O" uniqKey="Amano O">O. Amano</name></author><author><name sortKey="Iseki, S" uniqKey="Iseki S">S. Iseki</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belkina, N V" uniqKey="Belkina N">N. V. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Acta Histochem Cytochem</journal-id><journal-id journal-id-type="publisher-id">AHC</journal-id><journal-title-group><journal-title>Acta Histochemica et Cytochemica</journal-title></journal-title-group><issn pub-type="ppub">0044-5991</issn><issn pub-type="epub">1347-5800</issn><publisher><publisher-name>Japan Society of Histochemistry and Cytochemistry</publisher-name><publisher-loc>Tokyo, Japan</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">22282587</article-id><article-id pub-id-type="pmc">3263859</article-id><article-id pub-id-type="publisher-id">AHC11037</article-id><article-id pub-id-type="doi">10.1267/ahc.11037</article-id><article-categories><subj-group subj-group-type="heading"><subject>Regular Article</subject></subj-group></article-categories><title-group><article-title>Expression of Podoplanin and Classical Cadherins in Salivary Gland Epithelial Cells of Klotho-Deficient Mice</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Amano</surname><given-names>Ikuko</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Imaizumi</surname><given-names>Yuri</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kaji</surname><given-names>Chiaki</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Kojima</surname><given-names>Hiroshi</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Sawa</surname><given-names>Yoshihiko</given-names></name><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="corresp" rid="cor1"></xref></contrib></contrib-group><aff id="aff1"><label>1</label>Department of Oral Growth & Development, Fukuoka Dental College, Japan</aff><aff id="aff2"><label>2</label>Department of Morphological Biology, Fukuoka Dental College, Japan</aff><author-notes><corresp id="cor1">Correspondence to: Yoshihiko Sawa, Department of Morphological Biology, Fukuoka Dental College, 2–15–1 Tamura, Sawara-ku, Fukuoka 814–0193, Japan. E-mail: <email>ysawa@college.fdcnet.ac.jp</email></corresp></author-notes><pub-date pub-type="ppub"><day>28</day><month>12</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>5</day><month>11</month><year>2011</year></pub-date><volume>44</volume><issue>6</issue><fpage>267</fpage><lpage>276</lpage><history><date date-type="received"><day>3</day><month>8</month><year>2011</year></date><date date-type="accepted"><day>21</day><month>9</month><year>2011</year></date></history><permissions><copyright-statement>© 2011 The Japan Society of Histochemistry and Cytochemistry</copyright-statement><copyright-year>2011</copyright-year><license license-type="open-access"><license-p>This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p>We have recently shown that salivary gland myoepithelial cells express podoplanin. Podoplanin indirectly binds the actin filament network which links classical cadherins. The study here is aimed to investigate the expression of podoplanin and cadherins on salivary gland myoepithelial cells and the changes in the aging cells using klotho-deficient (kl/kl) mice. The submandibular glands of kl/kl mouse lack granular ducts which express klotho in wild type mice, suggesting that klotho may be a gene responsible for granular duct development. Although aging resulted in growth suppression of myoepithelial cells because of the sparse distribution of the cells in kl/kl mouse salivary glands, the expression of podoplanin and E-cadherin was shown in aging myoepithelial cells. It is thought that podoplanin participates in the actin-E-cadherin networks which are maintained in aging myoepithelial cells. It was also shown that granular ducts were filled with P-cadherin, and that the P-cadherin amount was larger in the wild type mouse submandibular glands than in the sublingual and parotid glands of wild type mouse, and in the submandibular glands of kl/kl mouse. These findings suggest that the granular duct is an organ secreting soluble P-cadherin into the saliva.</p></abstract><kwd-group><kwd>podoplanin</kwd><kwd>P-cadherin</kwd><kwd>N-cadherin</kwd><kwd>klotho</kwd><kwd>salivary gland</kwd></kwd-group></article-meta></front><floats-group><fig id="F1" position="float"><label>Fig. 1</label><caption><p>Macroscopic differences of submandibular glands between kl/kl and the wild type mice. Size of body and submandibular glands are significantly smaller in the kl/kl mice than in wild type mice at seven weeks after birth. Bar=25 mm (left) and 10 mm (right).</p></caption><graphic xlink:href="AHC11037f01"></graphic></fig><fig id="F2" position="float"><label>Fig. 2</label><caption><p>Immunostaining for α-SMA, and E-/P-cadherins of mouse tongue and breast. The H-E staining shows the lingual mucosa and mammary gland with lactating. Reaction products with anti-α-SMA are observed on blood vessels in tunica muscularis (arrows) and on mammary gland epithelia (arrowheads), whereas no cross reaction is observed on the lingual mucous epithelia and the intrinsic lingual muscle. Reaction products with anti-E-cadherin (upper figure) are observed on the lingual mucous epithelia at cell-cell contacts with no cross reaction in the tunica muscularis. Reaction products with anti-P-cadherin (lower figure) are observed on mammary gland epithelia as well as on milk in the latex ducts (asterisk). Bar=100 µm.</p></caption><graphic xlink:href="AHC11037f02"></graphic></fig><fig id="F3" position="float"><label>Fig. 3</label><caption><p>Immunostaining for podoplanin and α-SMA of mouse submandibular glands. The H-E staining shows that terminal secretory end pieces are composed of seromucous acinar cells with basophilic cytoplasm, and that striated ducts (SD) are composed of columnar cells with acidopilic cytoplasm in both kl/kl mice and wild type mice. In kl/kl mice, there are no granular ducts (GD) and the size of acini is significantly smaller than in wild type mice at seven weeks of age. The region of myoepithelial cells around acini and the basal side of ducts, which reacted with both anti-podoplanin and anti-α-SMA, is smaller and more sparsely in the submandibular gland lobule of kl/kl mice than in the wild type mice (arrows). More regions are immunostained with anti-α-SMA than with anti-podoplanin in both kl/kl mice and the wild type mice. The inside of acinar cells (asterisks) and the luminal side of striated ducts (arrowheads), and blood vessel walls (arrowhead) are reacted with anti-α-SMA, but not with anti-podoplanin. Bar=100 µm.</p></caption><graphic xlink:href="AHC11037f03"></graphic></fig><fig id="F4" position="float"><label>Fig. 4</label><caption><p>Immunostaining for klotho protein on mouse testis and major salivary glands. The H-E staining shows testis and acini of the three major salivary glands of wild type mice. In the testis reaction products with anti-klotho are found on Sertoli nursing cells (arrows), spermatid (arrowheads), and spermatogonia (asterisks). Reaction products with anti-klotho are not found on acinar cells in any of the salivary glands (asterisks). Reaction products with anti-klotho are also not found on striated ducts (SD) with acidopillic cytoplasm, and on intercalated ducts (IC), having a narrow lumen surrounded by cuboidal cells, but are found on a basal side of granular ducts (GD) (arrowhead). Bar=100 µm.</p></caption><graphic xlink:href="AHC11037f04"></graphic></fig><fig id="F5" position="float"><label>Fig. 5</label><caption><p>Immunostaining for podoplanin and E-cadherin of submandibular glands. The H-E staining shows terminal seromucous acini and striated ducts (SD) in kl/kl and wild type mice, and granular ducts (GD) in wild type mice. The region of myoepithelial cells around acini and ducts was immunostained with anti-podoplanin, and with anti-E-cadherin in both kl/kl and wild type mice (arrows). Epithelial cells of acini and ducts were also stained with anti-E-cadherin at cell-cell contacts where reaction products with anti-podoplanin were not found in kl/kl or wild type mice (arrowheads). Bar=100 µm.</p></caption><graphic xlink:href="AHC11037f05"></graphic></fig><fig id="F6" position="float"><label>Fig. 6</label><caption><p>Immunostaining of E-cadherin and P-cadherin in mouse major salivary glands of wild type mice. The H-E staining shows that the parotid gland of which terminal secretory end pieces of acini are composed of serous pyramidal cells and the striated duct (SD) are composed of columnar cells with acidopillic cytoplasm, the submandibular gland with basophilic seromucous acinar cells and granular ducts (GD), and the sublingual gland of which terminal secretory end pieces are composed of mucous-filled acinar cells. In all salivary glands, reaction products with anti-E-cadherin (arrows) are found at the area of myoepithelial cells around acini and ducts, and at cell-cell contacts of epithelial cells of acini and ducts. Reaction products with anti-P-cadherin (arrowheads) are found in nuclei, and around acini and ducts weakly in both parotid and sublingual glands while in the submandibular gland, granular ducts (GD) are filled with strong reaction products with anti-P-cadherin (arrowheads). Bar=100 µm.</p></caption><graphic xlink:href="AHC11037f06"></graphic></fig><fig id="F7" position="float"><label>Fig. 7</label><caption><p>Immunostaining of E-cadherin and P-cadherin in mouse major salivary glands of kl/kl mice. Reaction products with anti-E-cadherin (arrows) are found at the area of myoepithelial cells around acini and ducts, and at cell-cell contacts of epithelial cells of acini and striated ducts (SD) whereas reaction products with anti-P-cadherin are found in nuclei, and around acini and ducts weakly (arrowheads). Bar=100 µm.</p></caption><graphic xlink:href="AHC11037f07"></graphic></fig><fig id="F8" position="float"><label>Fig. 8</label><caption><p>P-cadherin mRNA and protein production in tissue of mouse salivary glands. (<bold>A</bold>) RT-PCR analysis: PCR products for β-actin mRNA are shown in the upper panel and for P-cadherin mRNA in the lower panel. No RT-PCR products of P-cadherin were detected from the tissue of wild type mouse sublingual glands (SLG) and parotid glands (PG), or from the tissue of kl/kl mouse submandibular gland (SMG) whereas they were detected from tissue of wild type mouse submandibular gland. MW, molecular weight marker. (<bold>B</bold>) Real-time PCR analysis. Relative mRNA amounts are expressed in arbitrary units. The P-cadherin mRNA amount in total RNA extracts from tissue is significantly larger in the submandibular gland (SMG) of wild type mice than in the sublingual (SLG) and parotid glands (PG) of wild type mice, and in SMG of kl/kl mice. *Statistically significantly difference. (<bold>C</bold>) Western blot analysis. The P-cadherin protein band (110 kDa) transferred onto the PVDF membrane was detected from the tissue of wild type mouse submandibular gland (SMG) and mammary gland with lactating (MG), but not from wild type mouse sublingual (SLG) and parotid glands (PG), or from SMG of kl/kl mice.</p></caption><graphic xlink:href="AHC11037f08"></graphic></fig></floats-group></pmc><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Amano, Ikuko" sort="Amano, Ikuko" uniqKey="Amano I" first="Ikuko" last="Amano">Ikuko Amano</name></noRegion><name sortKey="Imaizumi, Yuri" sort="Imaizumi, Yuri" uniqKey="Imaizumi Y" first="Yuri" last="Imaizumi">Yuri Imaizumi</name><name sortKey="Kaji, Chiaki" sort="Kaji, Chiaki" uniqKey="Kaji C" first="Chiaki" last="Kaji">Chiaki Kaji</name><name sortKey="Kojima, Hiroshi" sort="Kojima, Hiroshi" uniqKey="Kojima H" first="Hiroshi" last="Kojima">Hiroshi Kojima</name><name sortKey="Sawa, Yoshihiko" sort="Sawa, Yoshihiko" uniqKey="Sawa Y" first="Yoshihiko" last="Sawa">Yoshihiko Sawa</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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