Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens.
Identifieur interne : 003A14 ( Ncbi/Merge ); précédent : 003A13; suivant : 003A15Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens.
Auteurs : S. Janardhan [Inde] ; P. Pandiaraja ; V. Pandey ; A. Karande ; P. KalirajSource :
- Journal of helminthology [ 1475-2697 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (biosynthèse), Anticorps antihelminthe (immunologie), Anticorps monoclonaux (biosynthèse), Anticorps monoclonaux (immunologie), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (sang), Filariose lymphatique (diagnostic), Filariose lymphatique (immunologie), Filariose lymphatique (parasitologie), Humains, Protéines d'helminthes (génétique), Protéines d'helminthes (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Similitude de séquences d'acides aminés, Souris, Spécificité des anticorps, Test ELISA, Wuchereria bancrofti (immunologie).
- MESH :
- biosynthèse : Anticorps antihelminthe, Anticorps monoclonaux.
- diagnostic : Filariose lymphatique.
- génétique : Antigènes d'helminthe, Protéines d'helminthes, Protéines recombinantes.
- immunologie : Anticorps antihelminthe, Anticorps monoclonaux, Antigènes d'helminthe, Filariose lymphatique, Protéines d'helminthes, Protéines recombinantes, Wuchereria bancrofti.
- parasitologie : Filariose lymphatique.
- sang : Antigènes d'helminthe.
- Animaux, Humains, Similitude de séquences d'acides aminés, Souris, Spécificité des anticorps, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (biosynthesis), Antibodies, Helminth (immunology), Antibodies, Monoclonal (biosynthesis), Antibodies, Monoclonal (immunology), Antibody Specificity, Antigens, Helminth (blood), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (parasitology), Enzyme-Linked Immunosorbent Assay, Helminth Proteins (genetics), Helminth Proteins (immunology), Humans, Mice, Recombinant Proteins (genetics), Recombinant Proteins (immunology), Sequence Homology, Amino Acid, Wuchereria bancrofti (immunology).
- MESH :
- chemical , biosynthesis : Antibodies, Helminth, Antibodies, Monoclonal.
- chemical , blood : Antigens, Helminth.
- chemical , genetics : Antigens, Helminth, Helminth Proteins, Recombinant Proteins.
- chemical , immunology : Antibodies, Helminth, Antibodies, Monoclonal, Antigens, Helminth, Helminth Proteins, Recombinant Proteins.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Wuchereria bancrofti.
- parasitology : Elephantiasis, Filarial.
- Animals, Antibody Specificity, Enzyme-Linked Immunosorbent Assay, Humans, Mice, Sequence Homology, Amino Acid.
Abstract
The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the World Health Organization. Presently, few immunodiagnostics are available for filarial monitoring programmes. The Wuchereria bancrofti (Wb) SXP-1 parasite protein, with 84% homology to Brugia malayi (Bm) SXP-1, was found to be highly immunogenic. WbSXP-1 is one among the diagnostic candidate molecules that were used for developing a rapid-antibody-flow-through diagnostic kit for filariasis. Studies were initiated with the aim of developing monoclonal antibodies against recombinant WbSXP-1 and prospective applications for the detection of both circulating Wb and Bm antigens in serum samples from infected individuals. The monoclones 1A6C2 of subclass IgG1k, and 2A12F8 of class IgM, specifically detected Wb and Bm microfilaria isolated from patients and did not show cross-reactivity with other filarial recombinant antigens. We anticipate that this work will address the problems faced in the rapid diagnosis of human lymphatic filariasis in endemic areas in developing countries.
DOI: 10.1017/S0022149X10000118
PubMed: 20338077
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pubmed:20338077Le document en format XML
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We anticipate that this work will address the problems faced in the rapid diagnosis of human lymphatic filariasis in endemic areas in developing countries.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20338077</PMID><DateCreated><Year>2011</Year><Month>01</Month><Day>28</Day></DateCreated><DateCompleted><Year>2011</Year><Month>05</Month><Day>11</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1475-2697</ISSN><JournalIssue CitedMedium="Internet"><Volume>85</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Journal of helminthology</Title><ISOAbbreviation>J. Helminthol.</ISOAbbreviation></Journal><ArticleTitle>Development and characterization of monoclonal antibodies against WbSXP-1 for the detection of circulating filarial antigens.</ArticleTitle><Pagination><MedlinePgn>1-6</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1017/S0022149X10000118</ELocationID><Abstract><AbstractText>The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the World Health Organization. Presently, few immunodiagnostics are available for filarial monitoring programmes. The Wuchereria bancrofti (Wb) SXP-1 parasite protein, with 84% homology to Brugia malayi (Bm) SXP-1, was found to be highly immunogenic. WbSXP-1 is one among the diagnostic candidate molecules that were used for developing a rapid-antibody-flow-through diagnostic kit for filariasis. Studies were initiated with the aim of developing monoclonal antibodies against recombinant WbSXP-1 and prospective applications for the detection of both circulating Wb and Bm antigens in serum samples from infected individuals. The monoclones 1A6C2 of subclass IgG1k, and 2A12F8 of class IgM, specifically detected Wb and Bm microfilaria isolated from patients and did not show cross-reactivity with other filarial recombinant antigens. We anticipate that this work will address the problems faced in the rapid diagnosis of human lymphatic filariasis in endemic areas in developing countries.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Janardhan</LastName><ForeName>S</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Centre for Biotechnology, Anna University, Chennai 600025, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pandiaraja</LastName><ForeName>P</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Pandey</LastName><ForeName>V</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Karande</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Kaliraj</LastName><ForeName>P</ForeName><Initials>P</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Studies</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>03</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Helminthol</MedlineTA><NlmUniqueID>2985115R</NlmUniqueID><ISSNLinking>0022-149X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000909">Antibodies, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000911">Antibodies, Monoclonal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000947">Antigens, Helminth</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015801">Helminth Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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PubStatus="pubmed"><Year>2010</Year><Month>3</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>5</Month><Day>12</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20338077</ArticleId><ArticleId IdType="pii">S0022149X10000118</ArticleId><ArticleId IdType="doi">10.1017/S0022149X10000118</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name><name sortKey="Karande, A" sort="Karande, A" uniqKey="Karande A" first="A" last="Karande">A. Karande</name><name sortKey="Pandey, V" sort="Pandey, V" uniqKey="Pandey V" first="V" last="Pandey">V. Pandey</name><name sortKey="Pandiaraja, P" sort="Pandiaraja, P" uniqKey="Pandiaraja P" first="P" last="Pandiaraja">P. Pandiaraja</name></noCountry><country name="Inde"><noRegion><name sortKey="Janardhan, S" sort="Janardhan, S" uniqKey="Janardhan S" first="S" last="Janardhan">S. Janardhan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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