N-butanol Extract from Melilotus Suaveolens Ledeb Affects Pro- and Anti-Inflammatory Cytokines and Mediators
Identifieur interne : 003032 ( Ncbi/Merge ); précédent : 003031; suivant : 003033N-butanol Extract from Melilotus Suaveolens Ledeb Affects Pro- and Anti-Inflammatory Cytokines and Mediators
Auteurs : Lei Zhao ; Jun-Yan Tao ; Shu-Ling Zhang ; Feng Jin ; Ran Pang ; Ji-Hua Dong [République populaire de Chine]Source :
- Evidence-based Complementary and Alternative Medicine : eCAM [ 1741-427X ] ; 2007.
Abstract
Url:
DOI: 10.1093/ecam/nem165
PubMed: 18955281
PubMed Central: 2816390
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-butanol Extract from <italic>Melilotus Suaveolens Ledeb</italic>
Affects Pro- and Anti-Inflammatory Cytokines and Mediators</title>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main"><italic>N</italic>
-butanol Extract from <italic>Melilotus Suaveolens Ledeb</italic>
Affects Pro- and Anti-Inflammatory Cytokines and Mediators</title>
<author><name sortKey="Zhao, Lei" sort="Zhao, Lei" uniqKey="Zhao L" first="Lei" last="Zhao">Lei Zhao</name>
<affiliation><nlm:aff id="AFF1">Department of Hepatology & Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022,</nlm:aff>
<wicri:noCountry code="subfield">430022</wicri:noCountry>
</affiliation>
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<author><name sortKey="Tao, Jun Yan" sort="Tao, Jun Yan" uniqKey="Tao J" first="Jun-Yan" last="Tao">Jun-Yan Tao</name>
<affiliation><nlm:aff id="AFF1">College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430072,</nlm:aff>
<wicri:noCountry code="subfield">430072</wicri:noCountry>
</affiliation>
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<author><name sortKey="Zhang, Shu Ling" sort="Zhang, Shu Ling" uniqKey="Zhang S" first="Shu-Ling" last="Zhang">Shu-Ling Zhang</name>
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<wicri:noCountry code="subfield">430022</wicri:noCountry>
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<author><name sortKey="Jin, Feng" sort="Jin, Feng" uniqKey="Jin F" first="Feng" last="Jin">Feng Jin</name>
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<author><name sortKey="Pang, Ran" sort="Pang, Ran" uniqKey="Pang R" first="Ran" last="Pang">Ran Pang</name>
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<wicri:noCountry code="subfield">430022</wicri:noCountry>
</affiliation>
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<author><name sortKey="Dong, Ji Hua" sort="Dong, Ji Hua" uniqKey="Dong J" first="Ji-Hua" last="Dong">Ji-Hua Dong</name>
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<series><title level="j">Evidence-based Complementary and Alternative Medicine : eCAM</title>
<idno type="ISSN">1741-427X</idno>
<idno type="eISSN">1741-4288</idno>
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<front><div type="abstract" xml:lang="en"><p><italic>Melilotus suaveolens Ledeb</italic>
is a traditional medicinal plant for treating inflammation-related disease. This explores the inner anti-inflammatory mechanism of <italic>n</italic>
-butanol extract from <italic>M. suaveolens Ledeb</italic>
. Inflammatory cellular model was established by lipopolysaccharide intervention on RAW264.7 cell line. Levels of secreted cytokines TNF-α, IL-1β, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-α, COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, activation of NF-κB and ingredients in the extract were assayed by ELISA, real time quantitative PCR, western blot, immunocytochemical test and HPLC fingerprint test, respectively. As a result, the extract could not only markedly reduce the production of pro-inflammatory mediators to different extents by blocking NF-κB activation but also promote the release of anti-inflammatory mediator HO-1 significantly. Each 1 g extract contained 0.023531 mg coumarin and another two high polar ingredients, probably saponins. It can be concluded that the extract has similar effects on antagonizing pro-inflammatory mediators and cytokines like Dexamethasone, and has effects on promoting the production of anti-inflammatory mediators.</p>
</div>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Evid Based Complement Alternat Med</journal-id>
<journal-id journal-id-type="publisher-id">ecam</journal-id>
<journal-id journal-id-type="hwp">ecam</journal-id>
<journal-title-group><journal-title>Evidence-based Complementary and Alternative Medicine : eCAM</journal-title>
</journal-title-group>
<issn pub-type="ppub">1741-427X</issn>
<issn pub-type="epub">1741-4288</issn>
<publisher><publisher-name>Oxford University Press</publisher-name>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">18955281</article-id>
<article-id pub-id-type="pmc">2816390</article-id>
<article-id pub-id-type="doi">10.1093/ecam/nem165</article-id>
<article-id pub-id-type="publisher-id">nem165</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Articles - Basic Science</subject>
</subj-group>
</article-categories>
<title-group><article-title><italic>N</italic>
-butanol Extract from <italic>Melilotus Suaveolens Ledeb</italic>
Affects Pro- and Anti-Inflammatory Cytokines and Mediators</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Zhao</surname>
<given-names>Lei</given-names>
</name>
<xref ref-type="aff" rid="AFF1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tao</surname>
<given-names>Jun-Yan</given-names>
</name>
<xref ref-type="aff" rid="AFF1"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Zhang</surname>
<given-names>Shu-Ling</given-names>
</name>
<xref ref-type="aff" rid="AFF1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Jin</surname>
<given-names>Feng</given-names>
</name>
<xref ref-type="aff" rid="AFF1"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Pang</surname>
<given-names>Ran</given-names>
</name>
<xref ref-type="aff" rid="AFF1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Dong</surname>
<given-names>Ji-Hua</given-names>
</name>
<xref ref-type="aff" rid="AFF1"><sup>4</sup>
</xref>
</contrib>
</contrib-group>
<aff id="AFF1"><sup>1</sup>
Department of Hepatology & Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022,<sup>2</sup>
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430072,<sup>3</sup>
Department of Neurosurgery and<sup>4</sup>
Central Lab, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, P.R. China</aff>
<author-notes><corresp>For reprints and all Correspondence: Prof. Shu-Ling Zhang, Department of Hepatology & Infectious Disease, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, P.R. China. Tel: <phone>+86-27-62629209</phone>
; Fax: <fax>+86-27-85756636</fax>
; E-mail: <email>chineseomd@yahoo.com</email>
</corresp>
<fn><p>The first two authors have contributed equally to this work.</p>
</fn>
</author-notes>
<pub-date pub-type="ppub"><month>3</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="epub"><day>14</day>
<month>11</month>
<year>2007</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>14</day>
<month>11</month>
<year>2007</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
. </pmc-comment>
<volume>7</volume>
<issue>1</issue>
<fpage>97</fpage>
<lpage>106</lpage>
<history><date date-type="received"><day>18</day>
<month>6</month>
<year>2007</year>
</date>
<date date-type="accepted"><day>20</day>
<month>8</month>
<year>2007</year>
</date>
</history>
<permissions><copyright-statement>© 2007 The Author(s).</copyright-statement>
<license license-type="creative-commons" xlink:href="http://creativecommons.org/licenses/by-nc/2.0/uk/"><license-p><pmc-comment>CREATIVE COMMONS</pmc-comment>
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (<ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/2.0/uk/">http://creativecommons.org/licenses/by-nc/2.0/uk/</ext-link>
) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p>
</license>
</permissions>
<abstract><p><italic>Melilotus suaveolens Ledeb</italic>
is a traditional medicinal plant for treating inflammation-related disease. This explores the inner anti-inflammatory mechanism of <italic>n</italic>
-butanol extract from <italic>M. suaveolens Ledeb</italic>
. Inflammatory cellular model was established by lipopolysaccharide intervention on RAW264.7 cell line. Levels of secreted cytokines TNF-α, IL-1β, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-α, COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, activation of NF-κB and ingredients in the extract were assayed by ELISA, real time quantitative PCR, western blot, immunocytochemical test and HPLC fingerprint test, respectively. As a result, the extract could not only markedly reduce the production of pro-inflammatory mediators to different extents by blocking NF-κB activation but also promote the release of anti-inflammatory mediator HO-1 significantly. Each 1 g extract contained 0.023531 mg coumarin and another two high polar ingredients, probably saponins. It can be concluded that the extract has similar effects on antagonizing pro-inflammatory mediators and cytokines like Dexamethasone, and has effects on promoting the production of anti-inflammatory mediators.</p>
</abstract>
<kwd-group><kwd><italic>Melilotus suaveolens Ledeb</italic>
</kwd>
<kwd><italic>n</italic>
-butanol extract</kwd>
<kwd>RAW 264.7 cell line</kwd>
<kwd>NF-κB</kwd>
<kwd>HO-1</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><noCountry><name sortKey="Jin, Feng" sort="Jin, Feng" uniqKey="Jin F" first="Feng" last="Jin">Feng Jin</name>
<name sortKey="Pang, Ran" sort="Pang, Ran" uniqKey="Pang R" first="Ran" last="Pang">Ran Pang</name>
<name sortKey="Tao, Jun Yan" sort="Tao, Jun Yan" uniqKey="Tao J" first="Jun-Yan" last="Tao">Jun-Yan Tao</name>
<name sortKey="Zhang, Shu Ling" sort="Zhang, Shu Ling" uniqKey="Zhang S" first="Shu-Ling" last="Zhang">Shu-Ling Zhang</name>
<name sortKey="Zhao, Lei" sort="Zhao, Lei" uniqKey="Zhao L" first="Lei" last="Zhao">Lei Zhao</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Dong, Ji Hua" sort="Dong, Ji Hua" uniqKey="Dong J" first="Ji-Hua" last="Dong">Ji-Hua Dong</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
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