Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to Brugia malayi Wolbachia Surface Protein
Identifieur interne : 002810 ( Ncbi/Merge ); précédent : 002809; suivant : 002811Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to Brugia malayi Wolbachia Surface Protein
Auteurs : Chantima Porksakorn [Thaïlande] ; Surang Nuchprayoon [Thaïlande] ; Kiwon Park [États-Unis] ; Alan L. Scott [États-Unis]Source :
- Mediators of Inflammation [ 0962-9351 ] ; 2007.
Abstract
Url:
DOI: 10.1155/2007/84318
PubMed: 17641731
PubMed Central: 1906711
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to <italic>Brugia malayi Wolbachia</italic> Surface Protein</title><author><name sortKey="Porksakorn, Chantima" sort="Porksakorn, Chantima" uniqKey="Porksakorn C" first="Chantima" last="Porksakorn">Chantima Porksakorn</name><affiliation wicri:level="1"><nlm:aff id="a1">Lymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Lymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author><author><name sortKey="Nuchprayoon, Surang" sort="Nuchprayoon, Surang" uniqKey="Nuchprayoon S" first="Surang" last="Nuchprayoon">Surang Nuchprayoon</name><affiliation wicri:level="1"><nlm:aff id="a1">Lymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Lymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author><author><name sortKey="Park, Kiwon" sort="Park, Kiwon" uniqKey="Park K" first="Kiwon" last="Park">Kiwon Park</name><affiliation wicri:level="1"><nlm:aff id="a2">Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205</wicri:regionArea><wicri:noRegion>MD 21205</wicri:noRegion></affiliation></author><author><name sortKey="Scott, Alan L" sort="Scott, Alan L" uniqKey="Scott A" first="Alan L." last="Scott">Alan L. Scott</name><affiliation wicri:level="1"><nlm:aff id="a2">Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205</wicri:regionArea><wicri:noRegion>MD 21205</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">17641731</idno><idno type="pmc">1906711</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1906711</idno><idno type="RBID">PMC:1906711</idno><idno type="doi">10.1155/2007/84318</idno><date when="2007">2007</date><idno type="wicri:Area/Pmc/Corpus">004191</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">004191</idno><idno type="wicri:Area/Pmc/Curation">004190</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">004190</idno><idno type="wicri:Area/Pmc/Checkpoint">003A98</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003A98</idno><idno type="wicri:Area/Ncbi/Merge">002810</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to <italic>Brugia malayi Wolbachia</italic> Surface Protein</title><author><name sortKey="Porksakorn, Chantima" sort="Porksakorn, Chantima" uniqKey="Porksakorn C" first="Chantima" last="Porksakorn">Chantima Porksakorn</name><affiliation wicri:level="1"><nlm:aff id="a1">Lymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Lymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author><author><name sortKey="Nuchprayoon, Surang" sort="Nuchprayoon, Surang" uniqKey="Nuchprayoon S" first="Surang" last="Nuchprayoon">Surang Nuchprayoon</name><affiliation wicri:level="1"><nlm:aff id="a1">Lymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand</nlm:aff><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Lymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330</wicri:regionArea><wicri:noRegion>Bangkok 10330</wicri:noRegion></affiliation></author><author><name sortKey="Park, Kiwon" sort="Park, Kiwon" uniqKey="Park K" first="Kiwon" last="Park">Kiwon Park</name><affiliation wicri:level="1"><nlm:aff id="a2">Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205</wicri:regionArea><wicri:noRegion>MD 21205</wicri:noRegion></affiliation></author><author><name sortKey="Scott, Alan L" sort="Scott, Alan L" uniqKey="Scott A" first="Alan L." last="Scott">Alan L. Scott</name><affiliation wicri:level="1"><nlm:aff id="a2">Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205</wicri:regionArea><wicri:noRegion>MD 21205</wicri:noRegion></affiliation></author></analytic><series><title level="j">Mediators of Inflammation</title><idno type="ISSN">0962-9351</idno><idno type="eISSN">1466-1861</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><italic>Wolbachia</italic>, an endosymbiotic bacterium found in most species of filarial parasites, is thought to play a significant role in inducing innate inflammatory responses in lymphatic filariasis patients. However, the <italic>Wolbachia</italic>-derived molecules that are recognized by the innate immune system have not yet been identified. In this study, we exposed the murine macrophage cell line RAW 264.7 to a recombinant form of the major <italic>Wolbachia</italic> surface protein (rWSP) to determine if WSP is capable of innately inducing cytokine transcription. Interleukin (IL)-1<italic>β</italic>, IL-6, and tumor necrosis factor (TNF) mRNAs were all upregulated by the rWSP stimulation in a dose-dependant manner. TNF transcription peaked at 3 hours, whereas IL-1<italic>β</italic> and IL-6 transcription peaked at 6 hours post-rWSP exposure. The levels of innate cytokine expression induced by a high-dose (9.0 <italic>μ</italic>g/mL) rWSP in the RAW 264.7 cells were comparable to the levels induced by 0.1 <italic>μ</italic>g/mL <italic>E. coli</italic>-derived lipopolysaccharides. Pretreatment of the rWSP with
proteinase-K drastically reduced IL-1<italic>β</italic>, IL-6, and TNF transcription. However, the proinflammatory response was not inhibited by polymyxin B treatment. These results strongly suggest that the major <italic>Wolbachia</italic> surface protein molecule WSP is an important inducer of innate immune responses during filarial infections.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Molyneux, Dh" uniqKey="Molyneux D">DH Molyneux</name></author><author><name sortKey="Bradley, M" uniqKey="Bradley M">M Bradley</name></author><author><name sortKey="Hoerauf, A" uniqKey="Hoerauf A">A Hoerauf</name></author><author><name sortKey="Kyelem, D" uniqKey="Kyelem D">D Kyelem</name></author><author><name sortKey="Taylor, Mj" uniqKey="Taylor M">MJ Taylor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Triteeraprapab, S" uniqKey="Triteeraprapab S">S Triteeraprapab</name></author><author><name sortKey="Songtrus, J" uniqKey="Songtrus J">J Songtrus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Triteeraprapab, S" 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sortKey="Daehnel, K" uniqKey="Daehnel K">K Daehnel</name></author><author><name sortKey="Gillette Ferguson, I" uniqKey="Gillette Ferguson I">I Gillette-Ferguson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kaisho, T" uniqKey="Kaisho T">T Kaisho</name></author><author><name sortKey="Akira, S" uniqKey="Akira S">S Akira</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Turner, Jd" uniqKey="Turner J">JD Turner</name></author><author><name sortKey="Mand, S" uniqKey="Mand S">S Mand</name></author><author><name sortKey="Debrah, Ay" uniqKey="Debrah A">AY Debrah</name></author></analytic></biblStruct></listBibl></div1></back></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Mediators Inflamm</journal-id><journal-id journal-id-type="iso-abbrev">Mediators Inflamm</journal-id><journal-id journal-id-type="publisher-id">MI</journal-id><journal-title-group><journal-title>Mediators of Inflammation</journal-title></journal-title-group><issn pub-type="ppub">0962-9351</issn><issn pub-type="epub">1466-1861</issn><publisher><publisher-name>Hindawi Publishing Corporation</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">17641731</article-id><article-id pub-id-type="pmc">1906711</article-id><article-id pub-id-type="doi">10.1155/2007/84318</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Proinflammatory Cytokine Gene Expression by Murine Macrophages in Response to <italic>Brugia malayi Wolbachia</italic> Surface Protein</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Porksakorn</surname><given-names>Chantima</given-names></name><xref ref-type="aff" rid="a1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nuchprayoon</surname><given-names>Surang</given-names></name><xref ref-type="aff" rid="a1"><sup>1</sup></xref><xref ref-type="corresp" rid="cor1">*</xref></contrib><contrib contrib-type="author"><name><surname>Park</surname><given-names>Kiwon</given-names></name><xref ref-type="aff" rid="a2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Scott</surname><given-names>Alan L.</given-names></name><xref ref-type="aff" rid="a2"><sup>2</sup></xref></contrib></contrib-group><aff id="a1"><sup>1</sup>Lymphatic Filariasis Research Unit, Department of Parasitology, and Chulalongkorn Medical Research Center, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand</aff><aff id="a2"><sup>2</sup>Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA</aff><author-notes><corresp id="cor1">*Surang Nuchprayoon: <email>fmedstt@md2.md.chula.ac.th</email></corresp></author-notes><pub-date pub-type="ppub"><year>2007</year></pub-date><pub-date pub-type="epub"><day>3</day><month>5</month><year>2007</year></pub-date><volume>2007</volume><elocation-id>84318</elocation-id><history><date date-type="received"><day>29</day><month>11</month><year>2006</year></date><date date-type="accepted"><day>26</day><month>2</month><year>2007</year></date></history><permissions><copyright-statement>Copyright © 2007 Chantima Porksakorn et al.</copyright-statement><copyright-year>2007</copyright-year><license xlink:href="https://creativecommons.org/licenses/by/3.0/"><license-p>This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</license-p></license></permissions><abstract><p><italic>Wolbachia</italic>, an endosymbiotic bacterium found in most species of filarial parasites, is thought to play a significant role in inducing innate inflammatory responses in lymphatic filariasis patients. However, the <italic>Wolbachia</italic>-derived molecules that are recognized by the innate immune system have not yet been identified. In this study, we exposed the murine macrophage cell line RAW 264.7 to a recombinant form of the major <italic>Wolbachia</italic> surface protein (rWSP) to determine if WSP is capable of innately inducing cytokine transcription. Interleukin (IL)-1<italic>β</italic>, IL-6, and tumor necrosis factor (TNF) mRNAs were all upregulated by the rWSP stimulation in a dose-dependant manner. TNF transcription peaked at 3 hours, whereas IL-1<italic>β</italic> and IL-6 transcription peaked at 6 hours post-rWSP exposure. The levels of innate cytokine expression induced by a high-dose (9.0 <italic>μ</italic>g/mL) rWSP in the RAW 264.7 cells were comparable to the levels induced by 0.1 <italic>μ</italic>g/mL <italic>E. coli</italic>-derived lipopolysaccharides. Pretreatment of the rWSP with
proteinase-K drastically reduced IL-1<italic>β</italic>, IL-6, and TNF transcription. However, the proinflammatory response was not inhibited by polymyxin B treatment. These results strongly suggest that the major <italic>Wolbachia</italic> surface protein molecule WSP is an important inducer of innate immune responses during filarial infections.</p></abstract></article-meta></front><floats-group><fig id="F1" position="float"><label>Figure 1</label><caption><p>Dose response of rWSP-induced IL-1<italic>β</italic>, IL-6, and TNF mRNAs production in murine macrophage RAW 264.7 cells. The macrophage cells were incubated with various concentrations of rWSP for 3 hours. The data
represent mean values and standard deviations of fold increase of
cytokine transcripts relative to negative control in log scale.
Significant differences to untreated controls (*<italic>P</italic> < .01
for IL-1<italic>β</italic> mRNA levels and <italic>P</italic> < .05 for IL-6 and TNF mRNA levels) and to rWSP-stimulated cells at 9 <italic>μ</italic>g/mL (**<italic>P</italic> < .05) are indicated.</p></caption><graphic xlink:href="MI2007-84318.001"></graphic></fig><fig id="F2" position="float"><label>Figure 2</label><caption><p>Time-course analysis of rWSP-induced IL-1â (a), IL-6 (b),
and TNF (c) mRNAs expression in murine macrophage RAW 264.7 cells.
Incubation of the macrophage cells with 9.0 <italic>μ</italic>g/mL rWSP
(black symbols) or 0.1 <italic>μ</italic>g/mL <italic>E. coli</italic> LPS (white
symbols) were performed at various time-points. The data represent
mean values and standard deviations of fold increase of cytokine
transcripts relative to negative control in log scale. Significant
differences to untreated controls are indicated as *<italic>P</italic>< .05. ** Significant differences were found between
rWSP-stimulated cells at 1.5 hours and 3 hours postexposure as
well as between rWSP-stimulated cells at 3 hours and 6 hours
postexposure (for TNF mRNA levels; <italic>P</italic> < .05), while
*** significant differences were found between rWSP-stimulated cells at 3 hours and 6 hours postexposure as well as between
rWSP-stimulated cells at 6 hours and 9 hours postexposure (for
IL-1<italic>β</italic> and IL-6 mRNA levels; <italic>P</italic> < .01).</p></caption><graphic xlink:href="MI2007-84318.002"></graphic></fig><fig id="F3" position="float"><label>Figure 3</label><caption><p>Effect of proteinase <italic>K</italic> treatment in rWSP-induced
IL-1â (a), IL-6 (b), and TNF (c) mRNAs expression in macrophage RAW 264.7
cells. The macrophage cells were incubated with 9 <italic>μ</italic>g/mL
rWSP (WSP), 9 <italic>μ</italic>g/mL rWSP pretreated with proteinase
K (WSPPK), 0.1 <italic>μ</italic>g/mL LPS (LPS), and
0.1 <italic>μ</italic>g/mL LPS pretreated with proteinase K (LPSPK) for 3
hours. The data represent mean values and standard deviations of
fold increase of cytokine transcripts relative to negative
control. Significant differences were found between WSP- and
WSPPK-treated cells (<italic>P</italic> < .05; indicated by **).</p></caption><graphic xlink:href="MI2007-84318.003"></graphic></fig><fig id="F4" position="float"><label>Figure 4</label><caption><p>Effect of polymyxin
B treatment in rWSP-induced TNF mRNA expression in
macrophage RAW 264.7 cells. The macrophage cells were incubated
with rWSP (WSP; 1 and 9 <italic>μ</italic>g/mL), polymyxin
B-treated rWSP (WSPPB), LPS (LPS; 0.1 <italic>μ</italic>g/mL), and
polymyxin B-treated LPS (LPSPB) for 3 hours. The data
represent mean values and standard deviations of fold increase of
cytokine transcripts relative to negative control. Significant
differences were found between LPS- and LPSPB-treated cells
(<italic>P</italic> < .01; indicated by **).</p></caption><graphic xlink:href="MI2007-84318.004"></graphic></fig></floats-group></pmc><affiliations><list><country><li>Thaïlande</li><li>États-Unis</li></country></list><tree><country name="Thaïlande"><noRegion><name sortKey="Porksakorn, Chantima" sort="Porksakorn, Chantima" uniqKey="Porksakorn C" first="Chantima" last="Porksakorn">Chantima Porksakorn</name></noRegion><name sortKey="Nuchprayoon, Surang" sort="Nuchprayoon, Surang" uniqKey="Nuchprayoon S" first="Surang" last="Nuchprayoon">Surang Nuchprayoon</name></country><country name="États-Unis"><noRegion><name sortKey="Park, Kiwon" sort="Park, Kiwon" uniqKey="Park K" first="Kiwon" last="Park">Kiwon Park</name></noRegion><name sortKey="Scott, Alan L" sort="Scott, Alan L" uniqKey="Scott A" first="Alan L." last="Scott">Alan L. Scott</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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