Autologous lymphocyte therapy for experimental canine lymphoedema: a pilot study.
Identifieur interne : 00B449 ( Ncbi/Curation ); précédent : 00B448; suivant : 00B450Autologous lymphocyte therapy for experimental canine lymphoedema: a pilot study.
Auteurs : K R Knight [Australie] ; M. Ritz ; D A Lepore ; R. Booth ; K. Octigan ; B M O'BrienSource :
- The Australian and New Zealand journal of surgery [ 0004-8682 ] ; 1994.
Descripteurs français
- KwdFr :
- Animaux, Chiens, Collagène (analyse), Eau corporelle (), Endopeptidases (métabolisme), Espace extracellulaire (métabolisme), Lymphocytes (imagerie diagnostique), Lymphoedème (), Peau (), Projets pilotes, Protéines (métabolisme), Résultat thérapeutique, Scintigraphie, Transfusion de lymphocytes, Transfusion sanguine autologue.
- MESH :
- analyse : Collagène.
- imagerie diagnostique : Lymphocytes.
- métabolisme : Endopeptidases, Espace extracellulaire, Protéines.
- Animaux, Chiens, Eau corporelle, Lymphoedème, Peau, Projets pilotes, Résultat thérapeutique, Scintigraphie, Transfusion de lymphocytes, Transfusion sanguine autologue.
English descriptors
- KwdEn :
- Animals, Blood Transfusion, Autologous, Body Water (chemistry), Collagen (analysis), Dogs, Endopeptidases (metabolism), Extracellular Space (metabolism), Lymphedema (therapy), Lymphocyte Transfusion, Lymphocytes (diagnostic imaging), Pilot Projects, Proteins (metabolism), Radionuclide Imaging, Skin (chemistry), Treatment Outcome.
- MESH :
- chemical , analysis : Collagen.
- chemistry : Body Water, Skin.
- diagnostic imaging : Lymphocytes.
- chemical , metabolism : Endopeptidases, Extracellular Space, Proteins.
- therapy : Lymphedema.
- Animals, Blood Transfusion, Autologous, Dogs, Lymphocyte Transfusion, Pilot Projects, Radionuclide Imaging, Treatment Outcome.
Abstract
Obstructive lymphoedema, an accumulation of protein-rich fluid in interstitial spaces, was created in five dogs by a combination of the irradiation of one groin and subsequent surgical ablation of any remaining lymphatics. The lymphoedema was stable for up to 2 years. The aim was to test the efficacy of intra-arterial injection of autologous lymphocytes as a therapy for lymphoedema. The hypothesis was that cytokines produced by lymphocytes mediate proteolysis by macrophage proteinases in the lymphoedematous limb to remove the excess protein and relieve the oedema. A concentrated lymphocyte-rich preparation was isolated from blood by the Ficoll-Paque method. These preparations were injected into the femoral artery four times at approximately 4 weekly intervals. Three months after the first injection of lymphocytes, lymphoedematous limbs showed a marked 69% reduction in the mean excess circumferences compared with opposite control limbs. After treatment, skin thickness and hydroxyproline content (both measures of fibrosis) as well as water content (a measure of oedema) had reduced significantly. In specimens of interstitial fluid and in skin homogenates acidic proteinase activity increased and the protein concentration decreased significantly compared with controls. It is concluded that increased proteolysis, possibly due to activated macrophages recruited to the lymphoedematous limb, may partly explain these results.
PubMed: 8179530
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Autologous lymphocyte therapy for experimental canine lymphoedema: a pilot study.</title><author><name sortKey="Knight, K R" sort="Knight, K R" uniqKey="Knight K" first="K R" last="Knight">K R Knight</name><affiliation wicri:level="1"><nlm:affiliation>Microsurgery Research Centre, St Vincent's Hospital, Melbourne, Victoria, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Microsurgery Research Centre, St Vincent's Hospital, Melbourne, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Ritz, M" sort="Ritz, M" uniqKey="Ritz M" first="M" last="Ritz">M. Ritz</name></author><author><name sortKey="Lepore, D A" sort="Lepore, D A" uniqKey="Lepore D" first="D A" last="Lepore">D A Lepore</name></author><author><name sortKey="Booth, R" sort="Booth, R" uniqKey="Booth R" first="R" last="Booth">R. Booth</name></author><author><name sortKey="Octigan, K" sort="Octigan, K" uniqKey="Octigan K" first="K" last="Octigan">K. Octigan</name></author><author><name sortKey="O Brien, B M" sort="O Brien, B M" uniqKey="O Brien B" first="B M" last="O'Brien">B M O'Brien</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1994">1994</date><idno type="RBID">pubmed:8179530</idno><idno type="pmid">8179530</idno><idno type="wicri:Area/PubMed/Corpus">005550</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">005550</idno><idno type="wicri:Area/PubMed/Curation">005550</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">005550</idno><idno type="wicri:Area/PubMed/Checkpoint">005550</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">005550</idno><idno type="wicri:Area/Ncbi/Merge">00B449</idno><idno type="wicri:Area/Ncbi/Curation">00B449</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Autologous lymphocyte therapy for experimental canine lymphoedema: a pilot study.</title><author><name sortKey="Knight, K R" sort="Knight, K R" uniqKey="Knight K" first="K R" last="Knight">K R Knight</name><affiliation wicri:level="1"><nlm:affiliation>Microsurgery Research Centre, St Vincent's Hospital, Melbourne, Victoria, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Microsurgery Research Centre, St Vincent's Hospital, Melbourne, Victoria</wicri:regionArea><wicri:noRegion>Victoria</wicri:noRegion></affiliation></author><author><name sortKey="Ritz, M" sort="Ritz, M" uniqKey="Ritz M" first="M" last="Ritz">M. Ritz</name></author><author><name sortKey="Lepore, D A" sort="Lepore, D A" uniqKey="Lepore D" first="D A" last="Lepore">D A Lepore</name></author><author><name sortKey="Booth, R" sort="Booth, R" uniqKey="Booth R" first="R" last="Booth">R. Booth</name></author><author><name sortKey="Octigan, K" sort="Octigan, K" uniqKey="Octigan K" first="K" last="Octigan">K. Octigan</name></author><author><name sortKey="O Brien, B M" sort="O Brien, B M" uniqKey="O Brien B" first="B M" last="O'Brien">B M O'Brien</name></author></analytic><series><title level="j">The Australian and New Zealand journal of surgery</title><idno type="ISSN">0004-8682</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Blood Transfusion, Autologous</term><term>Body Water (chemistry)</term><term>Collagen (analysis)</term><term>Dogs</term><term>Endopeptidases (metabolism)</term><term>Extracellular Space (metabolism)</term><term>Lymphedema (therapy)</term><term>Lymphocyte Transfusion</term><term>Lymphocytes (diagnostic imaging)</term><term>Pilot Projects</term><term>Proteins (metabolism)</term><term>Radionuclide Imaging</term><term>Skin (chemistry)</term><term>Treatment Outcome</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Chiens</term><term>Collagène (analyse)</term><term>Eau corporelle ()</term><term>Endopeptidases (métabolisme)</term><term>Espace extracellulaire (métabolisme)</term><term>Lymphocytes (imagerie diagnostique)</term><term>Lymphoedème ()</term><term>Peau ()</term><term>Projets pilotes</term><term>Protéines (métabolisme)</term><term>Résultat thérapeutique</term><term>Scintigraphie</term><term>Transfusion de lymphocytes</term><term>Transfusion sanguine autologue</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Collagen</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Collagène</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Body Water</term><term>Skin</term></keywords><keywords scheme="MESH" qualifier="diagnostic imaging" xml:lang="en"><term>Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="imagerie diagnostique" xml:lang="fr"><term>Lymphocytes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Endopeptidases</term><term>Extracellular Space</term><term>Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Endopeptidases</term><term>Espace extracellulaire</term><term>Protéines</term></keywords><keywords scheme="MESH" qualifier="therapy" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blood Transfusion, Autologous</term><term>Dogs</term><term>Lymphocyte Transfusion</term><term>Pilot Projects</term><term>Radionuclide Imaging</term><term>Treatment Outcome</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chiens</term><term>Eau corporelle</term><term>Lymphoedème</term><term>Peau</term><term>Projets pilotes</term><term>Résultat thérapeutique</term><term>Scintigraphie</term><term>Transfusion de lymphocytes</term><term>Transfusion sanguine autologue</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Obstructive lymphoedema, an accumulation of protein-rich fluid in interstitial spaces, was created in five dogs by a combination of the irradiation of one groin and subsequent surgical ablation of any remaining lymphatics. The lymphoedema was stable for up to 2 years. The aim was to test the efficacy of intra-arterial injection of autologous lymphocytes as a therapy for lymphoedema. The hypothesis was that cytokines produced by lymphocytes mediate proteolysis by macrophage proteinases in the lymphoedematous limb to remove the excess protein and relieve the oedema. A concentrated lymphocyte-rich preparation was isolated from blood by the Ficoll-Paque method. These preparations were injected into the femoral artery four times at approximately 4 weekly intervals. Three months after the first injection of lymphocytes, lymphoedematous limbs showed a marked 69% reduction in the mean excess circumferences compared with opposite control limbs. After treatment, skin thickness and hydroxyproline content (both measures of fibrosis) as well as water content (a measure of oedema) had reduced significantly. In specimens of interstitial fluid and in skin homogenates acidic proteinase activity increased and the protein concentration decreased significantly compared with controls. It is concluded that increased proteolysis, possibly due to activated macrophages recruited to the lymphoedematous limb, may partly explain these results.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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