Comparative study of Dot-immunogold silver staining and Dot-ELISA for the detection of serum antibodies against Wuchereria bancrofti.
Identifieur interne : 00B173 ( Ncbi/Curation ); précédent : 00B172; suivant : 00B174Comparative study of Dot-immunogold silver staining and Dot-ELISA for the detection of serum antibodies against Wuchereria bancrofti.
Auteurs : Y S Liu [République populaire de Chine] ; W P Du ; M. Chen ; Z X WuSource :
- The Southeast Asian journal of tropical medicine and public health [ 0125-1562 ] ; 1994.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Filariose lymphatique, Microfilaria, Wuchereria bancrofti.
- sang : Anticorps antihelminthe.
- Animaux, Humains, Immunohistochimie, Immunotransfert, Test ELISA, Valeur prédictive des tests.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Helminth.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Microfilariae, Wuchereria bancrofti.
- Animals, Enzyme-Linked Immunosorbent Assay, Humans, Immunoblotting, Immunohistochemistry, Predictive Value of Tests.
Abstract
Dot-immunogold silver staining (Dot-IGSS) and Dot-ELISA, using the soluble antigen of Brugia malayi, were employed to detect anti-Wuchereria bancrofti antibodies in 50 cases of Wuchereria bancrofti microfilaremia. The positive rates were 100% and 90% in Dot-IGSS and Dot-ELISA respectively. The average titer in the 45 positive cases was 1:184 (1:10-1:2560) for Dot-IGSS and 1:150 (1:10-1:2560) for Dot-ELISA, with 30 cases showing the same titer in both tests, 13 cases showing higher titer in Dot-IGSS than in Dot-ELISA and 2 cases in the former showing lower titers than in the latter. There was a linear relationship between the titers of antibodies detected by Dot-IGSS and by Dot-ELISA (r = 0.8443). Dot-IGSS, similar to Dot-ELISA, is easy to carry out and the result is easy to read. It is seen that Dot-IGSS is highly sensitive and specific and is practicable for immunodiagnosis and surveillance of filariasis.
PubMed: 7667722
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pubmed:7667722Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Helminth (blood)</term>
<term>Elephantiasis, Filarial (diagnosis)</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Immunoblotting</term>
<term>Immunohistochemistry</term>
<term>Microfilariae (immunology)</term>
<term>Predictive Value of Tests</term>
<term>Wuchereria bancrofti (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antihelminthe (sang)</term>
<term>Filariose lymphatique (diagnostic)</term>
<term>Filariose lymphatique (immunologie)</term>
<term>Humains</term>
<term>Immunohistochimie</term>
<term>Immunotransfert</term>
<term>Microfilaria (immunologie)</term>
<term>Test ELISA</term>
<term>Valeur prédictive des tests</term>
<term>Wuchereria bancrofti (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term>
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<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term>
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<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Filariose lymphatique</term>
<term>Microfilaria</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term>
<term>Microfilariae</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Humans</term>
<term>Immunoblotting</term>
<term>Immunohistochemistry</term>
<term>Predictive Value of Tests</term>
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<term>Humains</term>
<term>Immunohistochimie</term>
<term>Immunotransfert</term>
<term>Test ELISA</term>
<term>Valeur prédictive des tests</term>
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<front><div type="abstract" xml:lang="en">Dot-immunogold silver staining (Dot-IGSS) and Dot-ELISA, using the soluble antigen of Brugia malayi, were employed to detect anti-Wuchereria bancrofti antibodies in 50 cases of Wuchereria bancrofti microfilaremia. The positive rates were 100% and 90% in Dot-IGSS and Dot-ELISA respectively. The average titer in the 45 positive cases was 1:184 (1:10-1:2560) for Dot-IGSS and 1:150 (1:10-1:2560) for Dot-ELISA, with 30 cases showing the same titer in both tests, 13 cases showing higher titer in Dot-IGSS than in Dot-ELISA and 2 cases in the former showing lower titers than in the latter. There was a linear relationship between the titers of antibodies detected by Dot-IGSS and by Dot-ELISA (r = 0.8443). Dot-IGSS, similar to Dot-ELISA, is easy to carry out and the result is easy to read. It is seen that Dot-IGSS is highly sensitive and specific and is practicable for immunodiagnosis and surveillance of filariasis.</div>
</front>
</TEI>
</record>
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