Comparative study of Dot-immunogold silver staining and Dot-ELISA for the detection of serum antibodies against Wuchereria bancrofti.
Identifieur interne : 00B173 ( Ncbi/Curation ); précédent : 00B172; suivant : 00B174Comparative study of Dot-immunogold silver staining and Dot-ELISA for the detection of serum antibodies against Wuchereria bancrofti.
Auteurs : Y S Liu [République populaire de Chine] ; W P Du ; M. Chen ; Z X WuSource :
- The Southeast Asian journal of tropical medicine and public health [ 0125-1562 ] ; 1994.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Filariose lymphatique, Microfilaria, Wuchereria bancrofti.
- sang : Anticorps antihelminthe.
- Animaux, Humains, Immunohistochimie, Immunotransfert, Test ELISA, Valeur prédictive des tests.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Helminth.
- diagnosis : Elephantiasis, Filarial.
- immunology : Elephantiasis, Filarial, Microfilariae, Wuchereria bancrofti.
- Animals, Enzyme-Linked Immunosorbent Assay, Humans, Immunoblotting, Immunohistochemistry, Predictive Value of Tests.
Abstract
Dot-immunogold silver staining (Dot-IGSS) and Dot-ELISA, using the soluble antigen of Brugia malayi, were employed to detect anti-Wuchereria bancrofti antibodies in 50 cases of Wuchereria bancrofti microfilaremia. The positive rates were 100% and 90% in Dot-IGSS and Dot-ELISA respectively. The average titer in the 45 positive cases was 1:184 (1:10-1:2560) for Dot-IGSS and 1:150 (1:10-1:2560) for Dot-ELISA, with 30 cases showing the same titer in both tests, 13 cases showing higher titer in Dot-IGSS than in Dot-ELISA and 2 cases in the former showing lower titers than in the latter. There was a linear relationship between the titers of antibodies detected by Dot-IGSS and by Dot-ELISA (r = 0.8443). Dot-IGSS, similar to Dot-ELISA, is easy to carry out and the result is easy to read. It is seen that Dot-IGSS is highly sensitive and specific and is practicable for immunodiagnosis and surveillance of filariasis.
PubMed: 7667722
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pubmed:7667722Le document en format XML
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Chen</name></author><author><name sortKey="Wu, Z X" sort="Wu, Z X" uniqKey="Wu Z" first="Z X" last="Wu">Z X Wu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1994">1994</date><idno type="RBID">pubmed:7667722</idno><idno type="pmid">7667722</idno><idno type="wicri:Area/PubMed/Corpus">005473</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">005473</idno><idno type="wicri:Area/PubMed/Curation">005473</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">005473</idno><idno type="wicri:Area/PubMed/Checkpoint">005473</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">005473</idno><idno type="wicri:Area/Ncbi/Merge">00B173</idno><idno type="wicri:Area/Ncbi/Curation">00B173</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Comparative study of Dot-immunogold silver staining and Dot-ELISA for the detection of serum antibodies against Wuchereria bancrofti.</title><author><name sortKey="Liu, Y S" sort="Liu, Y S" uniqKey="Liu Y" first="Y S" last="Liu">Y S Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology, Xuzhou Medical College, Jiangsu, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Parasitology, Xuzhou Medical College, Jiangsu</wicri:regionArea><wicri:noRegion>Jiangsu</wicri:noRegion></affiliation></author><author><name sortKey="Du, W P" sort="Du, W P" uniqKey="Du W" first="W P" last="Du">W P Du</name></author><author><name sortKey="Chen, M" sort="Chen, M" uniqKey="Chen M" first="M" last="Chen">M. Chen</name></author><author><name sortKey="Wu, Z X" sort="Wu, Z X" uniqKey="Wu Z" first="Z X" last="Wu">Z X Wu</name></author></analytic><series><title level="j">The Southeast Asian journal of tropical medicine and public health</title><idno type="ISSN">0125-1562</idno><imprint><date when="1994" type="published">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Elephantiasis, Filarial (immunology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Immunoblotting</term><term>Immunohistochemistry</term><term>Microfilariae (immunology)</term><term>Predictive Value of Tests</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (sang)</term><term>Filariose lymphatique (diagnostic)</term><term>Filariose lymphatique (immunologie)</term><term>Humains</term><term>Immunohistochimie</term><term>Immunotransfert</term><term>Microfilaria (immunologie)</term><term>Test ELISA</term><term>Valeur prédictive des tests</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Filariose lymphatique</term><term>Microfilaria</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Microfilariae</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Humans</term><term>Immunoblotting</term><term>Immunohistochemistry</term><term>Predictive Value of Tests</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Immunohistochimie</term><term>Immunotransfert</term><term>Test ELISA</term><term>Valeur prédictive des tests</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Dot-immunogold silver staining (Dot-IGSS) and Dot-ELISA, using the soluble antigen of Brugia malayi, were employed to detect anti-Wuchereria bancrofti antibodies in 50 cases of Wuchereria bancrofti microfilaremia. The positive rates were 100% and 90% in Dot-IGSS and Dot-ELISA respectively. The average titer in the 45 positive cases was 1:184 (1:10-1:2560) for Dot-IGSS and 1:150 (1:10-1:2560) for Dot-ELISA, with 30 cases showing the same titer in both tests, 13 cases showing higher titer in Dot-IGSS than in Dot-ELISA and 2 cases in the former showing lower titers than in the latter. There was a linear relationship between the titers of antibodies detected by Dot-IGSS and by Dot-ELISA (r = 0.8443). Dot-IGSS, similar to Dot-ELISA, is easy to carry out and the result is easy to read. It is seen that Dot-IGSS is highly sensitive and specific and is practicable for immunodiagnosis and surveillance of filariasis.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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