Microcirculation-on-a-Chip: A Microfluidic Platform for Assaying Blood- and Lymphatic-Vessel Permeability
Identifieur interne : 007823 ( Ncbi/Curation ); précédent : 007822; suivant : 007824Microcirculation-on-a-Chip: A Microfluidic Platform for Assaying Blood- and Lymphatic-Vessel Permeability
Auteurs : Miwa Sato [Japon] ; Naoki Sasaki [Japon] ; Manabu Ato [Japon] ; Satoshi Hirakawa [Japon] ; Kiichi Sato [Japon] ; Kae Sato [Japon]Source :
- PLoS ONE [ 1932-6203 ] ; 2015.
Abstract
We developed a microfluidic model of microcirculation containing both blood and lymphatic vessels for examining vascular permeability. The designed microfluidic device harbors upper and lower channels that are partly aligned and are separated by a porous membrane, and on this membrane, blood vascular endothelial cells (BECs) and lymphatic endothelial cells (LECs) were cocultured back-to-back. At cell-cell junctions of both BECs and LECs, claudin-5 and VE-cadherin were detected. The permeability coefficient measured here was lower than the value reported for isolated mammalian venules. Moreover, our results showed that the flow culture established in the device promoted the formation of endothelial cell-cell junctions, and that treatment with histamine, an inflammation-promoting substance, induced changes in the localization of tight and adherens junction-associated proteins and an increase in vascular permeability in the microdevice. These findings indicated that both BECs and LECs appeared to retain their functions in the microfluidic coculture platform. Using this microcirculation device, the vascular damage induced by habu snake venom was successfully assayed, and the assay time was reduced from 24 h to 30 min. This is the first report of a microcirculation model in which BECs and LECs were cocultured. Because the micromodel includes lymphatic vessels in addition to blood vessels, the model can be used to evaluate both vascular permeability and lymphatic return rate.
Url:
DOI: 10.1371/journal.pone.0137301
PubMed: 26332321
PubMed Central: 4558006
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PMC:4558006Le document en format XML
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id="aff001"><addr-line>Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, Bunkyo, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, Bunkyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Sasaki, Naoki" sort="Sasaki, Naoki" uniqKey="Sasaki N" first="Naoki" last="Sasaki">Naoki Sasaki</name><affiliation wicri:level="3"><nlm:aff id="aff001"><addr-line>Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, Bunkyo, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, Bunkyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ato, Manabu" sort="Ato, Manabu" uniqKey="Ato M" first="Manabu" last="Ato">Manabu Ato</name><affiliation wicri:level="3"><nlm:aff id="aff002"><addr-line>Department of Immunology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Hirakawa, Satoshi" sort="Hirakawa, Satoshi" uniqKey="Hirakawa S" first="Satoshi" last="Hirakawa">Satoshi Hirakawa</name><affiliation wicri:level="1"><nlm:aff id="aff003"><addr-line>Department of Dermatology at Hamamatsu University School of Medicine, Hamamatsu city, Shizuoka, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Dermatology at Hamamatsu University School of Medicine, Hamamatsu city, Shizuoka</wicri:regionArea><wicri:noRegion>Shizuoka</wicri:noRegion></affiliation></author><author><name sortKey="Sato, Kiichi" sort="Sato, Kiichi" uniqKey="Sato K" first="Kiichi" last="Sato">Kiichi Sato</name><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Division of Molecular Science, School of Science and Technology, Gunma University, Kiryu, Gunma, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Molecular Science, School of Science and Technology, Gunma University, Kiryu, Gunma</wicri:regionArea><wicri:noRegion>Gunma</wicri:noRegion></affiliation></author><author><name sortKey="Sato, Kae" sort="Sato, Kae" uniqKey="Sato K" first="Kae" last="Sato">Kae Sato</name><affiliation wicri:level="3"><nlm:aff id="aff001"><addr-line>Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, Bunkyo, Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Chemical and Biological Sciences, Faculty of Science, Japan Women’s University, Bunkyo, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We developed a microfluidic model of microcirculation containing both blood and lymphatic vessels for examining vascular permeability. The designed microfluidic device harbors upper and lower channels that are partly aligned and are separated by a porous membrane, and on this membrane, blood vascular endothelial cells (BECs) and lymphatic endothelial cells (LECs) were cocultured back-to-back. At cell-cell junctions of both BECs and LECs, claudin-5 and VE-cadherin were detected. The permeability coefficient measured here was lower than the value reported for isolated mammalian venules. Moreover, our results showed that the flow culture established in the device promoted the formation of endothelial cell-cell junctions, and that treatment with histamine, an inflammation-promoting substance, induced changes in the localization of tight and adherens junction-associated proteins and an increase in vascular permeability in the microdevice. These findings indicated that both BECs and LECs appeared to retain their functions in the microfluidic coculture platform. Using this microcirculation device, the vascular damage induced by habu snake venom was successfully assayed, and the assay time was reduced from 24 h to 30 min. This is the first report of a microcirculation model in which BECs and LECs were cocultured. Because the micromodel includes lymphatic vessels in addition to blood vessels, the model can be used to evaluate both vascular permeability and lymphatic return rate.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Granger, Dn" uniqKey="Granger D">DN Granger</name></author><author><name sortKey="Senchenkova, E" uniqKey="Senchenkova E">E Senchenkova</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saito, Y" uniqKey="Saito Y">Y Saito</name></author><author><name sortKey="Nakagami, H" uniqKey="Nakagami H">H Nakagami</name></author><author><name sortKey="Kaneda, Y" uniqKey="Kaneda Y">Y Kaneda</name></author><author><name sortKey="Morishita, R" uniqKey="Morishita R">R Morishita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kano, Mr" uniqKey="Kano M">MR Kano</name></author><author><name sortKey="Komuta, Y" uniqKey="Komuta Y">Y Komuta</name></author><author><name sortKey="Iwata, C" uniqKey="Iwata C">C Iwata</name></author><author><name sortKey="Oka, M" uniqKey="Oka M">M Oka</name></author><author><name sortKey="Shirai, Yt" uniqKey="Shirai Y">YT Shirai</name></author><author><name sortKey="Morishita, Y" uniqKey="Morishita Y">Y Morishita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dixon, Jb" uniqKey="Dixon J">JB Dixon</name></author><author><name sortKey="Greiner, St" uniqKey="Greiner S">ST Greiner</name></author><author><name sortKey="Gashev, Aa" uniqKey="Gashev A">AA Gashev</name></author><author><name sortKey="Cote, Gl" uniqKey="Cote G">GL Cote</name></author><author><name sortKey="Moore, Je" uniqKey="Moore J">JE Moore</name></author><author><name sortKey="Zawieja, Dc" uniqKey="Zawieja D">DC Zawieja</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ono, N" uniqKey="Ono N">N Ono</name></author><author><name sortKey="Mizuno, R" uniqKey="Mizuno R">R Mizuno</name></author><author><name sortKey="Ohhashi, T" uniqKey="Ohhashi T">T Ohhashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Saria, A" uniqKey="Saria A">A Saria</name></author><author><name sortKey="Lundberg, Jm" uniqKey="Lundberg J">JM Lundberg</name></author><author><name sortKey="Skofitsch, G" uniqKey="Skofitsch G">G Skofitsch</name></author><author><name sortKey="Lembeck, F" uniqKey="Lembeck F">F Lembeck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laschke, Mw" uniqKey="Laschke M">MW Laschke</name></author><author><name sortKey="Vollmar, B" uniqKey="Vollmar B">B Vollmar</name></author><author><name sortKey="Menger, Md" uniqKey="Menger M">MD Menger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Daly, Sm" uniqKey="Daly S">SM Daly</name></author><author><name sortKey="Leahy, Mj" uniqKey="Leahy M">MJ Leahy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tsuji, T" uniqKey="Tsuji T">T Tsuji</name></author><author><name sortKey="Sasaki, Y" uniqKey="Sasaki Y">Y Sasaki</name></author><author><name sortKey="Tanaka, M" uniqKey="Tanaka M">M Tanaka</name></author><author><name sortKey="Hanabata, N" uniqKey="Hanabata N">N Hanabata</name></author><author><name sortKey="Hada, R" uniqKey="Hada R">R Hada</name></author><author><name sortKey="Munakata, A" uniqKey="Munakata A">A Munakata</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Margaris, Kn" uniqKey="Margaris K">KN Margaris</name></author><author><name sortKey="Black, Ra" uniqKey="Black R">RA Black</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kawai, Y" uniqKey="Kawai Y">Y Kawai</name></author><author><name sortKey="Yokoyama, Y" uniqKey="Yokoyama Y">Y Yokoyama</name></author><author><name sortKey="Kaidoh, M" uniqKey="Kaidoh M">M Kaidoh</name></author><author><name sortKey="Ohhashi, T" uniqKey="Ohhashi T">T Ohhashi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Breslin, Jw" uniqKey="Breslin J">JW Breslin</name></author><author><name sortKey="Kurtz, Km" uniqKey="Kurtz K">KM Kurtz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reed, Al" uniqKey="Reed A">AL Reed</name></author><author><name sortKey="Rowson, Sa" uniqKey="Rowson S">SA Rowson</name></author><author><name sortKey="Dixon, Jb" uniqKey="Dixon J">JB Dixon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Farmer, Pj" uniqKey="Farmer P">PJ Farmer</name></author><author><name sortKey="Bernier, Sg" uniqKey="Bernier S">SG Bernier</name></author><author><name sortKey="Lepage, A" uniqKey="Lepage A">A Lepage</name></author><author><name sortKey="Guillemette, G" uniqKey="Guillemette G">G Guillemette</name></author><author><name sortKey="Regoli, D" uniqKey="Regoli D">D Regoli</name></author><author><name sortKey="Sirois, P" uniqKey="Sirois P">P Sirois</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guo, M" uniqKey="Guo M">M Guo</name></author><author><name sortKey="Breslin, Jw" uniqKey="Breslin J">JW Breslin</name></author><author><name sortKey="Wu, Mh" uniqKey="Wu M">MH Wu</name></author><author><name sortKey="Gottardi, Cj" uniqKey="Gottardi C">CJ Gottardi</name></author><author><name sortKey="Yuan, Sy" uniqKey="Yuan S">SY Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Nieuw Amerongen, Gp" uniqKey="Van Nieuw Amerongen G">GP van Nieuw Amerongen</name></author><author><name sortKey="Draijer, R" uniqKey="Draijer R">R Draijer</name></author><author><name sortKey="Vermeer, Ma" uniqKey="Vermeer M">MA Vermeer</name></author><author><name sortKey="Van Hinsbergh, Vw" uniqKey="Van Hinsbergh V">VW van Hinsbergh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tanaka, Y" uniqKey="Tanaka Y">Y Tanaka</name></author><author><name sortKey="Kikukawa, Y" uniqKey="Kikukawa Y">Y Kikukawa</name></author><author><name sortKey="Sato, K" uniqKey="Sato K">K Sato</name></author><author><name sortKey="Sugii, Y" uniqKey="Sugii Y">Y Sugii</name></author><author><name sortKey="Kitamori, T" uniqKey="Kitamori T">T Kitamori</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sasaki, N" uniqKey="Sasaki N">N Sasaki</name></author><author><name sortKey="Shinjo, M" uniqKey="Shinjo M">M Shinjo</name></author><author><name sortKey="Hirakawa, S" uniqKey="Hirakawa S">S Hirakawa</name></author><author><name sortKey="Nishinaka, M" uniqKey="Nishinaka M">M Nishinaka</name></author><author><name sortKey="Tanaka, Y" uniqKey="Tanaka Y">Y Tanaka</name></author><author><name sortKey="Mawatari, K" uniqKey="Mawatari K">K Mawatari</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chrobak, Km" uniqKey="Chrobak K">KM Chrobak</name></author><author><name sortKey="Potter, Dr" uniqKey="Potter D">DR Potter</name></author><author><name sortKey="Tien, J" uniqKey="Tien J">J Tien</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Young, Ew" uniqKey="Young E">EW Young</name></author><author><name sortKey="Watson, Mw" uniqKey="Watson M">MW Watson</name></author><author><name sortKey="Srigunapalan, S" uniqKey="Srigunapalan S">S Srigunapalan</name></author><author><name sortKey="Wheeler, Ar" uniqKey="Wheeler A">AR Wheeler</name></author><author><name sortKey="Simmons, Ca" uniqKey="Simmons C">CA Simmons</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shao, J" uniqKey="Shao J">J Shao</name></author><author><name sortKey="Wu, L" uniqKey="Wu L">L Wu</name></author><author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name></author><author><name sortKey="Zheng, Y" uniqKey="Zheng Y">Y Zheng</name></author><author><name sortKey="Zhao, H" uniqKey="Zhao H">H Zhao</name></author><author><name sortKey="Lou, X" uniqKey="Lou X">X Lou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, Jw" uniqKey="Song J">JW Song</name></author><author><name sortKey="Cavnar, Sp" uniqKey="Cavnar S">SP Cavnar</name></author><author><name sortKey="Walker, Ac" uniqKey="Walker A">AC Walker</name></author><author><name sortKey="Luker, Ke" uniqKey="Luker K">KE Luker</name></author><author><name sortKey="Gupta, M" uniqKey="Gupta M">M Gupta</name></author><author><name sortKey="Tung, Yc" uniqKey="Tung Y">YC Tung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Srigunapalan, S" uniqKey="Srigunapalan S">S Srigunapalan</name></author><author><name sortKey="Lam, C" uniqKey="Lam C">C Lam</name></author><author><name sortKey="Wheeler, Ar" uniqKey="Wheeler A">AR Wheeler</name></author><author><name sortKey="Simmons, Ca" uniqKey="Simmons C">CA Simmons</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Mb" uniqKey="Chen M">MB Chen</name></author><author><name sortKey="Srigunapalan, S" uniqKey="Srigunapalan S">S Srigunapalan</name></author><author><name sortKey="Wheeler, Ar" uniqKey="Wheeler A">AR Wheeler</name></author><author><name sortKey="Simmons, Ca" uniqKey="Simmons C">CA Simmons</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wong, Kh" uniqKey="Wong K">KH Wong</name></author><author><name sortKey="Truslow, Jg" uniqKey="Truslow J">JG Truslow</name></author><author><name sortKey="Khankhel, Ah" uniqKey="Khankhel A">AH Khankhel</name></author><author><name sortKey="Chan, Kl" uniqKey="Chan K">KL Chan</name></author><author><name sortKey="Tien, J" uniqKey="Tien J">J Tien</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Song, Jw" uniqKey="Song J">JW Song</name></author><author><name sortKey="Munn, Ll" uniqKey="Munn L">LL Munn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yeon, Jh" uniqKey="Yeon J">JH Yeon</name></author><author><name sortKey="Ryu, Hr" uniqKey="Ryu H">HR Ryu</name></author><author><name sortKey="Chung, M" uniqKey="Chung M">M Chung</name></author><author><name sortKey="Hu, Qp" uniqKey="Hu Q">QP Hu</name></author><author><name sortKey="Jeon, Nl" uniqKey="Jeon N">NL Jeon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, S" uniqKey="Kim S">S Kim</name></author><author><name sortKey="Lee, H" uniqKey="Lee H">H Lee</name></author><author><name sortKey="Chung, M" uniqKey="Chung M">M Chung</name></author><author><name sortKey="Jeon, Nl" uniqKey="Jeon N">NL Jeon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Booth, R" uniqKey="Booth R">R Booth</name></author><author><name sortKey="Kim, H" uniqKey="Kim H">H Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Griep, Lm" uniqKey="Griep L">LM Griep</name></author><author><name sortKey="Wolbers, F" uniqKey="Wolbers F">F Wolbers</name></author><author><name sortKey="De Wagenaar, B" uniqKey="De Wagenaar B">B de Wagenaar</name></author><author><name sortKey="Ter Braak, Pm" uniqKey="Ter Braak P">PM ter Braak</name></author><author><name sortKey="Weksler, Bb" uniqKey="Weksler B">BB Weksler</name></author><author><name sortKey="Romero, Ia" uniqKey="Romero I">IA Romero</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Prabhakarpandian, B" uniqKey="Prabhakarpandian B">B Prabhakarpandian</name></author><author><name sortKey="Shen, Mc" uniqKey="Shen M">MC Shen</name></author><author><name sortKey="Nichols, Jb" uniqKey="Nichols J">JB Nichols</name></author><author><name sortKey="Mills, Ir" uniqKey="Mills I">IR Mills</name></author><author><name sortKey="Sidoryk Wegrzynowicz, M" uniqKey="Sidoryk Wegrzynowicz M">M Sidoryk-Wegrzynowicz</name></author><author><name sortKey="Aschner, M" uniqKey="Aschner M">M Aschner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Price, Gm" uniqKey="Price G">GM Price</name></author><author><name sortKey="Chrobak, Km" uniqKey="Chrobak K">KM Chrobak</name></author><author><name sortKey="Tien, J" uniqKey="Tien J">J Tien</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chan, Kl" uniqKey="Chan K">KL Chan</name></author><author><name sortKey="Khankhel, Ah" uniqKey="Khankhel A">AH Khankhel</name></author><author><name sortKey="Thompson, Rl" uniqKey="Thompson R">RL Thompson</name></author><author><name sortKey="Coisman, Bj" uniqKey="Coisman B">BJ Coisman</name></author><author><name sortKey="Wong, Kh" uniqKey="Wong K">KH Wong</name></author><author><name sortKey="Truslow, Jg" uniqKey="Truslow J">JG Truslow</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Chueh, Bh" uniqKey="Chueh B">BH Chueh</name></author><author><name sortKey="Huh, D" uniqKey="Huh D">D Huh</name></author><author><name sortKey="Kyrtsos, Cr" uniqKey="Kyrtsos C">CR Kyrtsos</name></author><author><name sortKey="Houssin, T" uniqKey="Houssin T">T Houssin</name></author><author><name sortKey="Futai, N" uniqKey="Futai N">N Futai</name></author><author><name sortKey="Takayama, S" uniqKey="Takayama S">S Takayama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Imura, Y" uniqKey="Imura Y">Y Imura</name></author><author><name sortKey="Asano, Y" uniqKey="Asano Y">Y Asano</name></author><author><name sortKey="Sato, K" uniqKey="Sato K">K Sato</name></author><author><name sortKey="Yoshimura, E" uniqKey="Yoshimura E">E Yoshimura</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Qi, D" uniqKey="Qi D">D Qi</name></author><author><name sortKey="Hoelzlea, Dj" uniqKey="Hoelzlea D">DJ Hoelzlea</name></author><author><name sortKey="Rowat, Ac" uniqKey="Rowat A">AC Rowat</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Der Meer, Ad" uniqKey="Van Der Meer A">AD van der Meer</name></author><author><name sortKey="Poot, Aa" uniqKey="Poot A">AA Poot</name></author><author><name sortKey="Feijen, J" uniqKey="Feijen J">J Feijen</name></author><author><name sortKey="Vermes, I" uniqKey="Vermes I">I Vermes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Breiteneder Geleff, S" uniqKey="Breiteneder Geleff S">S Breiteneder-Geleff</name></author><author><name sortKey="Soleiman, A" uniqKey="Soleiman A">A Soleiman</name></author><author><name sortKey="Kowalski, H" uniqKey="Kowalski H">H Kowalski</name></author><author><name sortKey="Horvat, R" uniqKey="Horvat R">R Horvat</name></author><author><name sortKey="Amann, G" uniqKey="Amann G">G Amann</name></author><author><name sortKey="Kriehuber, E" uniqKey="Kriehuber E">E Kriehuber</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Harris, Es" uniqKey="Harris E">ES Harris</name></author><author><name sortKey="Nelson, Wj" uniqKey="Nelson W">WJ Nelson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morita, K" uniqKey="Morita K">K Morita</name></author><author><name sortKey="Sasaki, H" uniqKey="Sasaki H">H Sasaki</name></author><author><name sortKey="Furuse, M" uniqKey="Furuse M">M Furuse</name></author><author><name sortKey="Tsukita, S" uniqKey="Tsukita S">S Tsukita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Booth, R" uniqKey="Booth R">R Booth</name></author><author><name sortKey="Kim, H" uniqKey="Kim H">H Kim</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, Mh" uniqKey="Wu M">MH Wu</name></author><author><name sortKey="Ustinova, E" uniqKey="Ustinova E">E Ustinova</name></author><author><name sortKey="Granger, Hj" uniqKey="Granger H">HJ Granger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zheng, Y" uniqKey="Zheng Y">Y Zheng</name></author><author><name sortKey="Chen, J" uniqKey="Chen J">J Chen</name></author><author><name sortKey="Craven, M" uniqKey="Craven M">M Craven</name></author><author><name sortKey="Choi, Nw" uniqKey="Choi N">NW Choi</name></author><author><name sortKey="Totorica, S" uniqKey="Totorica S">S Totorica</name></author><author><name sortKey="Diaz Santana, A" uniqKey="Diaz Santana A">A Diaz-Santana</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, Mb" uniqKey="Chen M">MB Chen</name></author><author><name sortKey="Whisler, Ja" uniqKey="Whisler J">JA Whisler</name></author><author><name sortKey="Jeon, Js" uniqKey="Jeon J">JS Jeon</name></author><author><name sortKey="Kamm, Rd" uniqKey="Kamm R">RD Kamm</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lee, H" uniqKey="Lee H">H Lee</name></author><author><name sortKey="Kim, S" uniqKey="Kim S">S Kim</name></author><author><name sortKey="Chung, M" uniqKey="Chung M">M Chung</name></author><author><name sortKey="Kim, Jh" uniqKey="Kim J">JH Kim</name></author><author><name sortKey="Jeon, Nl" uniqKey="Jeon N">NL Jeon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rigor, Rr" uniqKey="Rigor R">RR Rigor</name></author><author><name sortKey="Shen, Q" uniqKey="Shen Q">Q Shen</name></author><author><name sortKey="Pivetti, Cd" uniqKey="Pivetti C">CD Pivetti</name></author><author><name sortKey="Wu, Mh" uniqKey="Wu M">MH Wu</name></author><author><name sortKey="Yuan, Sy" uniqKey="Yuan S">SY Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kumar, P" uniqKey="Kumar P">P Kumar</name></author><author><name sortKey="Shen, Q" uniqKey="Shen Q">Q Shen</name></author><author><name sortKey="Pivetti, Cd" uniqKey="Pivetti C">CD Pivetti</name></author><author><name sortKey="Lee, Es" uniqKey="Lee E">ES Lee</name></author><author><name sortKey="Wu, Mh" uniqKey="Wu M">MH Wu</name></author><author><name sortKey="Yuan, Sy" uniqKey="Yuan S">SY Yuan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, C" uniqKey="Chen C">C Chen</name></author><author><name sortKey="Khismatullin, Db" uniqKey="Khismatullin D">DB Khismatullin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kondo, H" uniqKey="Kondo H">H Kondo</name></author><author><name sortKey="Kondo, S" uniqKey="Kondo S">S Kondo</name></author><author><name sortKey="Ikezawa, H" uniqKey="Ikezawa H">H Ikezawa</name></author><author><name sortKey="Murata, R" uniqKey="Murata R">R Murata</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Theakston, Rd" uniqKey="Theakston R">RD Theakston</name></author><author><name sortKey="Warrell, Da" uniqKey="Warrell D">DA Warrell</name></author><author><name sortKey="Griffiths, E" uniqKey="Griffiths E">E Griffiths</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Morine, N" uniqKey="Morine N">N Morine</name></author><author><name sortKey="Matsuda, S" uniqKey="Matsuda S">S Matsuda</name></author><author><name sortKey="Terada, K" uniqKey="Terada K">K Terada</name></author><author><name sortKey="Eto, A" uniqKey="Eto A">A Eto</name></author><author><name sortKey="Ishida, I" uniqKey="Ishida I">I Ishida</name></author><author><name sortKey="Oku, H" uniqKey="Oku H">H Oku</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thompson, Vf" uniqKey="Thompson V">VF Thompson</name></author><author><name sortKey="Saldana, S" uniqKey="Saldana S">S Saldana</name></author><author><name sortKey="Cong, J" uniqKey="Cong J">J Cong</name></author><author><name sortKey="Goll, De" uniqKey="Goll D">DE Goll</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Maeda, H" uniqKey="Maeda H">H Maeda</name></author><author><name sortKey="Nakamura, H" uniqKey="Nakamura H">H Nakamura</name></author><author><name sortKey="Fang, J" uniqKey="Fang J">J Fang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bertrand, N" uniqKey="Bertrand N">N Bertrand</name></author><author><name sortKey="Wu, J" uniqKey="Wu J">J Wu</name></author><author><name sortKey="Xu, X" uniqKey="Xu X">X Xu</name></author><author><name sortKey="Kamaly, N" uniqKey="Kamaly N">N Kamaly</name></author><author><name sortKey="Farokhzad, Oc" uniqKey="Farokhzad O">OC Farokhzad</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhang, Xy" uniqKey="Zhang X">XY Zhang</name></author><author><name sortKey="Lu, Wy" uniqKey="Lu W">WY Lu</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour 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