LymphedemaV1, Ncbi, Curation, bibRecord, 005619

[Determination of circulating filarial antigen using monoclonal antibody against Brugia malayi adult worm ES antigen with Dot-ELISA].

Identifieur interne : 005619 ( Ncbi/Curation ); précédent : 005618; suivant : 005620

[Determination of circulating filarial antigen using monoclonal antibody against Brugia malayi adult worm ES antigen with Dot-ELISA].

Auteurs : H J Zheng

Source :

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] [ 0253-9624 ] ; 1990.

RBID : pubmed:2340764

Descripteurs français

KwdFr :
- Animaux, Anticorps monoclonaux, Antigènes d'helminthe (analyse), Brugia (immunologie), Filariose lymphatique (immunologie), Hybridomes (sécrétion), Microfilaria (immunologie), Souris, Souris de lignée BALB C, Test ELISA ().
MESH :
- analyse : Antigènes d'helminthe.
- immunologie : Brugia, Filariose lymphatique, Microfilaria.
- sécrétion : Hybridomes.
- Animaux, Anticorps monoclonaux, Souris, Souris de lignée BALB C, Test ELISA.

English descriptors

KwdEn :
- Animals, Antibodies, Monoclonal, Antigens, Helminth (analysis), Brugia (immunology), Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay (methods), Hybridomas (secretion), Mice, Mice, Inbred BALB C, Microfilariae (immunology).
MESH :
- chemical , analysis : Antigens, Helminth.
- chemical : Antibodies, Monoclonal.
- immunology : Brugia, Elephantiasis, Filarial, Microfilariae.
- methods : Enzyme-Linked Immunosorbent Assay.
- secretion : Hybridomas.
- Animals, Mice, Mice, Inbred BALB C.

Abstract

A hybridoma cell line secreting monoclonal antibody (McAb) against Brugia malayi adult worm excretory-secretory antigen (AWES) was established. The identification of the Ig sub-classes showed that McAb AWES belongs to IgG. We developed a Dot-enzyme-linked immunosorbent assay (Dot-ELISA) to detect circulating parasite antigens in human lymphatic filariasis. 75 of 77 (97.4%) bancroftian microfilaremia cases showed positive reaction. 27 out of 40 (67.5%) microfilarial patients with hydroceles, chyluria, or elephantiasis, and 20% of sera from asymptomatic residents of filariasis-endemic areas evidently contained filarial antigens. 31 of sera from non-endemic area with ascaris infection were all negative. The minimal amount of antigens in pool normal sera adding AWES antigen was detectable at 6.3 pg/ml. The results suggested that Dot-ELISA is a sensitive, specific, cheap and simple agent for use in epidemiological field studies.

PubMed: 2340764

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H J Zheng

<affiliation><nlm:affiliation>Guizhou Provincial Institute of Parasitic Diseases, Guiyang.</nlm:affiliation>
<wicri:noCountry code="subField">Guiyang</wicri:noCountry>
</affiliation>

Le document en format XML

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<author><name sortKey="Zheng, H J" sort="Zheng, H J" uniqKey="Zheng H" first="H J" last="Zheng">H J Zheng</name>
<affiliation><nlm:affiliation>Guizhou Provincial Institute of Parasitic Diseases, Guiyang.</nlm:affiliation>
<wicri:noCountry code="subField">Guiyang</wicri:noCountry>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Determination of circulating filarial antigen using monoclonal antibody against Brugia malayi adult worm ES antigen with Dot-ELISA].</title>
<author><name sortKey="Zheng, H J" sort="Zheng, H J" uniqKey="Zheng H" first="H J" last="Zheng">H J Zheng</name>
<affiliation><nlm:affiliation>Guizhou Provincial Institute of Parasitic Diseases, Guiyang.</nlm:affiliation>
<wicri:noCountry code="subField">Guiyang</wicri:noCountry>
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<series><title level="j">Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]</title>
<idno type="ISSN">0253-9624</idno>
<imprint><date when="1990" type="published">1990</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Monoclonal</term>
<term>Antigens, Helminth (analysis)</term>
<term>Brugia (immunology)</term>
<term>Elephantiasis, Filarial (immunology)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Hybridomas (secretion)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Microfilariae (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps monoclonaux</term>
<term>Antigènes d'helminthe (analyse)</term>
<term>Brugia (immunologie)</term>
<term>Filariose lymphatique (immunologie)</term>
<term>Hybridomes (sécrétion)</term>
<term>Microfilaria (immunologie)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Test ELISA ()</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Helminth</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes d'helminthe</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Brugia</term>
<term>Filariose lymphatique</term>
<term>Microfilaria</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia</term>
<term>Elephantiasis, Filarial</term>
<term>Microfilariae</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
</keywords>
<keywords scheme="MESH" qualifier="secretion" xml:lang="en"><term>Hybridomas</term>
</keywords>
<keywords scheme="MESH" qualifier="sécrétion" xml:lang="fr"><term>Hybridomes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Anticorps monoclonaux</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Test ELISA</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A hybridoma cell line secreting monoclonal antibody (McAb) against Brugia malayi adult worm excretory-secretory antigen (AWES) was established. The identification of the Ig sub-classes showed that McAb AWES belongs to IgG. We developed a Dot-enzyme-linked immunosorbent assay (Dot-ELISA) to detect circulating parasite antigens in human lymphatic filariasis. 75 of 77 (97.4%) bancroftian microfilaremia cases showed positive reaction. 27 out of 40 (67.5%) microfilarial patients with hydroceles, chyluria, or elephantiasis, and 20% of sera from asymptomatic residents of filariasis-endemic areas evidently contained filarial antigens. 31 of sera from non-endemic area with ascaris infection were all negative. The minimal amount of antigens in pool normal sera adding AWES antigen was detectable at 6.3 pg/ml. The results suggested that Dot-ELISA is a sensitive, specific, cheap and simple agent for use in epidemiological field studies.</div>
</front>
</TEI>
</record>

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[Determination of circulating filarial antigen using monoclonal antibody against Brugia malayi adult worm ES antigen with Dot-ELISA].

[Determination of circulating filarial antigen using monoclonal antibody against Brugia malayi adult worm ES antigen with Dot-ELISA].

Source :

Descripteurs français

English descriptors

Abstract

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Curation

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Le document en format XML

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