Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus
Identifieur interne : 002A62 ( Ncbi/Curation ); précédent : 002A61; suivant : 002A63Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus
Auteurs : George F. Mayhew [États-Unis] ; Lyric C. Bartholomay [États-Unis] ; Hang-Yen Kou [Taïwan] ; Thomas A. Rocheleau [États-Unis] ; Jeremy F. Fuchs [États-Unis] ; Matthew T. Aliota [États-Unis] ; I-Yu Tsao [Taïwan] ; Chiung-Yen Huang [Taïwan] ; Tze-Tze Liu [Taïwan] ; Kwang-Jen Hsiao [Taïwan] ; Shih-Feng Tsai [Taïwan] ; Ueng-Cheng Yang [Taïwan] ; Nicole T. Perna [États-Unis] ; Wen-Long Cho [Taïwan] ; Bruce M. Christensen [États-Unis] ; Cheng-Chen Chen [Taïwan]Source :
- BMC Genomics [ 1471-2164 ] ; 2007.
Descripteurs français
- KwdFr :
- Aedes (génétique), Animaux, Anopheles gambiae (génétique), Banque de gènes, Bases de données d'acides nucléiques, Brugia malayi (immunologie), Brugia malayi (pathogénicité), Culicidae (génétique), Culicidae (immunologie), Culicidae (parasitologie), Drosophila melanogaster (génétique), Famille multigénique, Femelle, Filariose lymphatique (transmission), Gènes d'insecte, Génome d'insecte, Humains, Immunité innée, Spécificité d'espèce, Vecteurs insectes (génétique), Vecteurs insectes (immunologie), Vecteurs insectes (parasitologie), Étiquettes de séquences exprimées.
- MESH :
- génétique : Aedes, Anopheles gambiae, Culicidae, Drosophila melanogaster, Vecteurs insectes.
- immunologie : Brugia malayi, Culicidae, Vecteurs insectes.
- parasitologie : Culicidae, Vecteurs insectes.
- pathogénicité : Brugia malayi.
- transmission : Animaux, Banque de gènes, Bases de données d'acides nucléiques, Famille multigénique, Femelle, Filariose lymphatique, Gènes d'insecte, Génome d'insecte, Humains, Immunité innée, Spécificité d'espèce, Étiquettes de séquences exprimées.
English descriptors
- KwdEn :
- Aedes (genetics), Animals, Anopheles gambiae (genetics), Brugia malayi (immunology), Brugia malayi (pathogenicity), Culicidae (genetics), Culicidae (immunology), Culicidae (parasitology), Databases, Nucleic Acid, Drosophila melanogaster (genetics), Elephantiasis, Filarial (transmission), Expressed Sequence Tags, Female, Gene Library, Genes, Insect, Genome, Insect, Humans, Immunity, Innate, Insect Vectors (genetics), Insect Vectors (immunology), Insect Vectors (parasitology), Multigene Family, Species Specificity.
- MESH :
- genetics : Aedes, Anopheles gambiae, Culicidae, Drosophila melanogaster, Insect Vectors.
- immunology : Brugia malayi, Culicidae, Insect Vectors.
- parasitology : Culicidae, Insect Vectors.
- pathogenicity : Brugia malayi.
- transmission : Elephantiasis, Filarial.
- Animals, Databases, Nucleic Acid, Expressed Sequence Tags, Female, Gene Library, Genes, Insect, Genome, Insect, Humans, Immunity, Innate, Multigene Family, Species Specificity.
Abstract
The mosquito,
Six libraries were constructed and used to generate 44,940 expressed sequence tags, of which 38,079 passed quality filters to be included in the annotation project and subsequent analyses. All of these sequences were collapsed into clusters resulting in 8,020 unique sequence clusters or singletons. EST clusters were annotated and curated manually within ASAP (A Systematic Annotation Package for Community Analysis of Genomes) web portal according to BLAST results from comparisons to Genbank, and the
The resulting dataset is the first of its kind for this mosquito vector and provides a basis for future studies of mosquito vectors regarding the cascade of events that occurs in response to infection, and thereby providing insight into vector competence and innate immunity.
Url:
DOI: 10.1186/1471-2164-8-462
PubMed: 18088419
PubMed Central: 2262096
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001B20
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :001B19
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :003B73
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :003442
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :003442
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :003442
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :002A62
Links to Exploration step
PMC:2262096Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Construction and characterization of an expressed sequenced tag library for the mosquito vector <italic>Armigeres subalbatus</italic></title><author><name sortKey="Mayhew, George F" sort="Mayhew, George F" uniqKey="Mayhew G" first="George F" last="Mayhew">George F. Mayhew</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Bartholomay, Lyric C" sort="Bartholomay, Lyric C" uniqKey="Bartholomay L" first="Lyric C" last="Bartholomay">Lyric C. Bartholomay</name><affiliation wicri:level="4"><nlm:aff id="I2">Department of Entomology, Iowa State University, Ames IA, 50010, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Entomology, Iowa State University, Ames IA, 50010</wicri:regionArea><orgName type="university">Université d'État de l'Iowa</orgName><placeName><settlement type="city">Ames (Iowa)</settlement><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Kou, Hang Yen" sort="Kou, Hang Yen" uniqKey="Kou H" first="Hang-Yen" last="Kou">Hang-Yen Kou</name><affiliation wicri:level="1"><nlm:aff id="I3">Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Rocheleau, Thomas A" sort="Rocheleau, Thomas A" uniqKey="Rocheleau T" first="Thomas A" last="Rocheleau">Thomas A. Rocheleau</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Fuchs, Jeremy F" sort="Fuchs, Jeremy F" uniqKey="Fuchs J" first="Jeremy F" last="Fuchs">Jeremy F. Fuchs</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Aliota, Matthew T" sort="Aliota, Matthew T" uniqKey="Aliota M" first="Matthew T" last="Aliota">Matthew T. Aliota</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Tsao, I Yu" sort="Tsao, I Yu" uniqKey="Tsao I" first="I-Yu" last="Tsao">I-Yu Tsao</name><affiliation wicri:level="1"><nlm:aff id="I3">Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Chiung Yen" sort="Huang, Chiung Yen" uniqKey="Huang C" first="Chiung-Yen" last="Huang">Chiung-Yen Huang</name><affiliation wicri:level="1"><nlm:aff id="I3">Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Tze Tze" sort="Liu, Tze Tze" uniqKey="Liu T" first="Tze-Tze" last="Liu">Tze-Tze Liu</name><affiliation wicri:level="1"><nlm:aff id="I4">VYM Genome Research Center, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>VYM Genome Research Center, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Hsiao, Kwang Jen" sort="Hsiao, Kwang Jen" uniqKey="Hsiao K" first="Kwang-Jen" last="Hsiao">Kwang-Jen Hsiao</name><affiliation wicri:level="1"><nlm:aff id="I5">Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Tsai, Shih Feng" sort="Tsai, Shih Feng" uniqKey="Tsai S" first="Shih-Feng" last="Tsai">Shih-Feng Tsai</name><affiliation wicri:level="1"><nlm:aff id="I4">VYM Genome Research Center, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>VYM Genome Research Center, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="I6">Division of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli County 350, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Division of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli County 350</wicri:regionArea><wicri:noRegion>Miaoli County 350</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Ueng Cheng" sort="Yang, Ueng Cheng" uniqKey="Yang U" first="Ueng-Cheng" last="Yang">Ueng-Cheng Yang</name><affiliation wicri:level="1"><nlm:aff id="I7">Institute of Bioinformatics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Bioinformatics, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Perna, Nicole T" sort="Perna, Nicole T" uniqKey="Perna N" first="Nicole T" last="Perna">Nicole T. Perna</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Cho, Wen Long" sort="Cho, Wen Long" uniqKey="Cho W" first="Wen-Long" last="Cho">Wen-Long Cho</name><affiliation wicri:level="1"><nlm:aff id="I3">Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Christensen, Bruce M" sort="Christensen, Bruce M" uniqKey="Christensen B" first="Bruce M" last="Christensen">Bruce M. Christensen</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Cheng Chen" sort="Chen, Cheng Chen" uniqKey="Chen C" first="Cheng-Chen" last="Chen">Cheng-Chen Chen</name><affiliation wicri:level="1"><nlm:aff id="I3">Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18088419</idno><idno type="pmc">2262096</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2262096</idno><idno type="RBID">PMC:2262096</idno><idno type="doi">10.1186/1471-2164-8-462</idno><date when="2007">2007</date><idno type="wicri:Area/Pmc/Corpus">001B20</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001B20</idno><idno type="wicri:Area/Pmc/Curation">001B19</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001B19</idno><idno type="wicri:Area/Pmc/Checkpoint">003B73</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003B73</idno><idno type="wicri:source">PubMed</idno><idno type="wicri:Area/PubMed/Corpus">003442</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003442</idno><idno type="wicri:Area/PubMed/Curation">003442</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003442</idno><idno type="wicri:Area/PubMed/Checkpoint">003442</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003442</idno><idno type="wicri:Area/Ncbi/Merge">002A62</idno><idno type="wicri:Area/Ncbi/Curation">002A62</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Construction and characterization of an expressed sequenced tag library for the mosquito vector <italic>Armigeres subalbatus</italic></title><author><name sortKey="Mayhew, George F" sort="Mayhew, George F" uniqKey="Mayhew G" first="George F" last="Mayhew">George F. Mayhew</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Bartholomay, Lyric C" sort="Bartholomay, Lyric C" uniqKey="Bartholomay L" first="Lyric C" last="Bartholomay">Lyric C. Bartholomay</name><affiliation wicri:level="4"><nlm:aff id="I2">Department of Entomology, Iowa State University, Ames IA, 50010, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Entomology, Iowa State University, Ames IA, 50010</wicri:regionArea><orgName type="university">Université d'État de l'Iowa</orgName><placeName><settlement type="city">Ames (Iowa)</settlement><region type="state">Iowa</region></placeName></affiliation></author><author><name sortKey="Kou, Hang Yen" sort="Kou, Hang Yen" uniqKey="Kou H" first="Hang-Yen" last="Kou">Hang-Yen Kou</name><affiliation wicri:level="1"><nlm:aff id="I3">Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Rocheleau, Thomas A" sort="Rocheleau, Thomas A" uniqKey="Rocheleau T" first="Thomas A" last="Rocheleau">Thomas A. Rocheleau</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Fuchs, Jeremy F" sort="Fuchs, Jeremy F" uniqKey="Fuchs J" first="Jeremy F" last="Fuchs">Jeremy F. Fuchs</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Aliota, Matthew T" sort="Aliota, Matthew T" uniqKey="Aliota M" first="Matthew T" last="Aliota">Matthew T. Aliota</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Tsao, I Yu" sort="Tsao, I Yu" uniqKey="Tsao I" first="I-Yu" last="Tsao">I-Yu Tsao</name><affiliation wicri:level="1"><nlm:aff id="I3">Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Chiung Yen" sort="Huang, Chiung Yen" uniqKey="Huang C" first="Chiung-Yen" last="Huang">Chiung-Yen Huang</name><affiliation wicri:level="1"><nlm:aff id="I3">Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Tze Tze" sort="Liu, Tze Tze" uniqKey="Liu T" first="Tze-Tze" last="Liu">Tze-Tze Liu</name><affiliation wicri:level="1"><nlm:aff id="I4">VYM Genome Research Center, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>VYM Genome Research Center, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Hsiao, Kwang Jen" sort="Hsiao, Kwang Jen" uniqKey="Hsiao K" first="Kwang-Jen" last="Hsiao">Kwang-Jen Hsiao</name><affiliation wicri:level="1"><nlm:aff id="I5">Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Medical Research and Education, Taipei Veterans General Hospital, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Tsai, Shih Feng" sort="Tsai, Shih Feng" uniqKey="Tsai S" first="Shih-Feng" last="Tsai">Shih-Feng Tsai</name><affiliation wicri:level="1"><nlm:aff id="I4">VYM Genome Research Center, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>VYM Genome Research Center, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="I6">Division of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli County 350, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Division of Molecular and Genomic Medicine, National Health Research Institutes, Miaoli County 350</wicri:regionArea><wicri:noRegion>Miaoli County 350</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Ueng Cheng" sort="Yang, Ueng Cheng" uniqKey="Yang U" first="Ueng-Cheng" last="Yang">Ueng-Cheng Yang</name><affiliation wicri:level="1"><nlm:aff id="I7">Institute of Bioinformatics, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Bioinformatics, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Perna, Nicole T" sort="Perna, Nicole T" uniqKey="Perna N" first="Nicole T" last="Perna">Nicole T. Perna</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Cho, Wen Long" sort="Cho, Wen Long" uniqKey="Cho W" first="Wen-Long" last="Cho">Wen-Long Cho</name><affiliation wicri:level="1"><nlm:aff id="I3">Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author><author><name sortKey="Christensen, Bruce M" sort="Christensen, Bruce M" uniqKey="Christensen B" first="Bruce M" last="Christensen">Bruce M. Christensen</name><affiliation wicri:level="1"><nlm:aff id="I1">Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathobiological Sciences, University of Wisconsin-Madison, 1656 Linden Dr., Madison Wisconsin, 53706</wicri:regionArea><wicri:noRegion>53706</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Cheng Chen" sort="Chen, Cheng Chen" uniqKey="Chen C" first="Cheng-Chen" last="Chen">Cheng-Chen Chen</name><affiliation wicri:level="1"><nlm:aff id="I3">Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112, Taiwan</nlm:aff><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Tropical Medicine, National Yang-Ming University, Shih-Pai, Taipei 112</wicri:regionArea><wicri:noRegion>Taipei 112</wicri:noRegion></affiliation></author></analytic><series><title level="j">BMC Genomics</title><idno type="eISSN">1471-2164</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aedes (genetics)</term><term>Animals</term><term>Anopheles gambiae (genetics)</term><term>Brugia malayi (immunology)</term><term>Brugia malayi (pathogenicity)</term><term>Culicidae (genetics)</term><term>Culicidae (immunology)</term><term>Culicidae (parasitology)</term><term>Databases, Nucleic Acid</term><term>Drosophila melanogaster (genetics)</term><term>Elephantiasis, Filarial (transmission)</term><term>Expressed Sequence Tags</term><term>Female</term><term>Gene Library</term><term>Genes, Insect</term><term>Genome, Insect</term><term>Humans</term><term>Immunity, Innate</term><term>Insect Vectors (genetics)</term><term>Insect Vectors (immunology)</term><term>Insect Vectors (parasitology)</term><term>Multigene Family</term><term>Species Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aedes (génétique)</term><term>Animaux</term><term>Anopheles gambiae (génétique)</term><term>Banque de gènes</term><term>Bases de données d'acides nucléiques</term><term>Brugia malayi (immunologie)</term><term>Brugia malayi (pathogénicité)</term><term>Culicidae (génétique)</term><term>Culicidae (immunologie)</term><term>Culicidae (parasitologie)</term><term>Drosophila melanogaster (génétique)</term><term>Famille multigénique</term><term>Femelle</term><term>Filariose lymphatique (transmission)</term><term>Gènes d'insecte</term><term>Génome d'insecte</term><term>Humains</term><term>Immunité innée</term><term>Spécificité d'espèce</term><term>Vecteurs insectes (génétique)</term><term>Vecteurs insectes (immunologie)</term><term>Vecteurs insectes (parasitologie)</term><term>Étiquettes de séquences exprimées</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Aedes</term><term>Anopheles gambiae</term><term>Culicidae</term><term>Drosophila melanogaster</term><term>Insect Vectors</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Aedes</term><term>Anopheles gambiae</term><term>Culicidae</term><term>Drosophila melanogaster</term><term>Vecteurs insectes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Brugia malayi</term><term>Culicidae</term><term>Vecteurs insectes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Culicidae</term><term>Insect Vectors</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Culicidae</term><term>Vecteurs insectes</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Culicidae</term><term>Insect Vectors</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Databases, Nucleic Acid</term><term>Expressed Sequence Tags</term><term>Female</term><term>Gene Library</term><term>Genes, Insect</term><term>Genome, Insect</term><term>Humans</term><term>Immunity, Innate</term><term>Multigene Family</term><term>Species Specificity</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="fr"><term>Animaux</term><term>Banque de gènes</term><term>Bases de données d'acides nucléiques</term><term>Famille multigénique</term><term>Femelle</term><term>Filariose lymphatique</term><term>Gènes d'insecte</term><term>Génome d'insecte</term><term>Humains</term><term>Immunité innée</term><term>Spécificité d'espèce</term><term>Étiquettes de séquences exprimées</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>The mosquito, <italic>Armigeres subalbatus</italic>, mounts a distinctively robust innate immune response when infected with the nematode <italic>Brugia malayi</italic>, a causative agent of lymphatic filariasis. In order to mine the transcriptome for new insight into the cascade of events that takes place in response to infection in this mosquito, 6 cDNA libraries were generated from tissues of adult female mosquitoes subjected to immune-response activation treatments that lead to well-characterized responses, and from aging, naïve mosquitoes. Expressed sequence tags (ESTs) from each library were produced, annotated, and subjected to comparative analyses.</p></sec><sec><title>Results</title><p>Six libraries were constructed and used to generate 44,940 expressed sequence tags, of which 38,079 passed quality filters to be included in the annotation project and subsequent analyses. All of these sequences were collapsed into clusters resulting in 8,020 unique sequence clusters or singletons. EST clusters were annotated and curated manually within ASAP (A Systematic Annotation Package for Community Analysis of Genomes) web portal according to BLAST results from comparisons to Genbank, and the <italic>Anopheles gambiae </italic>and <italic>Drosophila melanogaster </italic>genome projects.</p></sec><sec><title>Conclusion</title><p>The resulting dataset is the first of its kind for this mosquito vector and provides a basis for future studies of mosquito vectors regarding the cascade of events that occurs in response to infection, and thereby providing insight into vector competence and innate immunity.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002A62 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 002A62 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Ncbi
|étape= Curation
|type= RBID
|clé= PMC:2262096
|texte= Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:18088419" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |