LymphedemaV1, Ncbi, Curation, bibRecord, 002543

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Identifieur interne : 002543 ( Ncbi/Curation ); précédent : 002542; suivant : 002544

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Auteurs : Ekaterina I. Galanzha ; Valery V. Tuchin ; Vladimir P. Zharov

Source :

World Journal of Gastroenterology : WJG [ 1007-9327 ] ; 2007.

RBID : PMC:4065947

Descripteurs français

KwdFr :
- Animaux, Cellules sanguines (physiologie), Circulation sanguine, Cytométrie en flux (), Microscopie (), Modèles animaux, Mésentère (cytologie), Mésentère (physiologie), Rats, Système lymphatique (cytologie), Système lymphatique (physiologie), Évaluation préclinique de médicament ().
MESH :
- cytologie : Mésentère, Système lymphatique.
- physiologie : Cellules sanguines, Mésentère, Système lymphatique.
- Animaux, Circulation sanguine, Cytométrie en flux, Microscopie, Modèles animaux, Rats, Évaluation préclinique de médicament.

English descriptors

KwdEn :
- Animals, Blood Cells (physiology), Blood Circulation, Drug Evaluation, Preclinical (methods), Flow Cytometry (methods), Lymphatic System (cytology), Lymphatic System (physiology), Mesentery (cytology), Mesentery (physiology), Microscopy (methods), Models, Animal, Rats.
MESH :
- cytology : Lymphatic System, Mesentery.
- methods : Drug Evaluation, Preclinical, Flow Cytometry, Microscopy.
- physiology : Blood Cells, Lymphatic System, Mesentery.
- Animals, Blood Circulation, Models, Animal, Rats.

Abstract

Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo. Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell’s functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo- and radio-sensitivity tests, and drug screening, are also discussed.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4065947

DOI: 10.3748/wjg.v13.i2.192
PubMed: 17226898
PubMed Central: 4065947

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PMC:4065947

Le document en format XML

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 flow cytometry, dynamic microscopy, and drug screening</title>
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<series><title level="j">World Journal of Gastroenterology : WJG</title>
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<term>Drug Evaluation, Preclinical (methods)</term>
<term>Flow Cytometry (methods)</term>
<term>Lymphatic System (cytology)</term>
<term>Lymphatic System (physiology)</term>
<term>Mesentery (cytology)</term>
<term>Mesentery (physiology)</term>
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<term>Models, Animal</term>
<term>Rats</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules sanguines (physiologie)</term>
<term>Circulation sanguine</term>
<term>Cytométrie en flux ()</term>
<term>Microscopie ()</term>
<term>Modèles animaux</term>
<term>Mésentère (cytologie)</term>
<term>Mésentère (physiologie)</term>
<term>Rats</term>
<term>Système lymphatique (cytologie)</term>
<term>Système lymphatique (physiologie)</term>
<term>Évaluation préclinique de médicament ()</term>
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<term>Système lymphatique</term>
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<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Lymphatic System</term>
<term>Mesentery</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Drug Evaluation, Preclinical</term>
<term>Flow Cytometry</term>
<term>Microscopy</term>
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<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Cellules sanguines</term>
<term>Mésentère</term>
<term>Système lymphatique</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Blood Cells</term>
<term>Lymphatic System</term>
<term>Mesentery</term>
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<term>Rats</term>
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<front><div type="abstract" xml:lang="en"><p>Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation <italic>in vivo</italic>
. Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including <italic>in vivo</italic>
 label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell’s functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including <italic>in vivo</italic>
 early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo- and radio-sensitivity tests, and drug screening, are also discussed.</p>
</div>
</front>
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Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki