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In vitro production of IgG4 by peripheral blood mononuclear cells (PBMC): the contribution of committed B cells

Identifieur interne : 00BD22 ( Ncbi/Checkpoint ); précédent : 00BD21; suivant : 00BD23

In vitro production of IgG4 by peripheral blood mononuclear cells (PBMC): the contribution of committed B cells

Auteurs : B A De Boer ; Y C M. Kruize ; M. Yazdanbakhsh

Source :

Clinical and Experimental Immunology [ 0009-9104 ] ; 1998.

RBID : PMC:1905099

Descripteurs français

KwdFr :
- ARN messager, Adulte, Agranulocytes (immunologie), Agranulocytes (métabolisme), Animaux, Chaines gamma des immunoglobulines (génétique), Filariose lymphatique (immunologie), Filariose lymphatique (sang), Humains, Immunoglobuline D, Immunoglobuline E (sang), Immunoglobuline G (biosynthèse), Immunoglobuline G (sang), Interleukine-13 (métabolisme), Interleukine-4 (métabolisme), Lymphocytes B (immunologie), Région switch des immunoglobulines.
MESH :
- biosynthèse : Immunoglobuline G.
- génétique : Chaines gamma des immunoglobulines.
- immunologie : Agranulocytes, Filariose lymphatique, Lymphocytes B.
- métabolisme : Agranulocytes, Interleukine-13, Interleukine-4.
- sang : Filariose lymphatique, Immunoglobuline E, Immunoglobuline G.
- ARN messager, Adulte, Animaux, Humains, Immunoglobuline D, Région switch des immunoglobulines.

English descriptors

KwdEn :
- Adult, Animals, B-Lymphocytes (immunology), Elephantiasis, Filarial (blood), Elephantiasis, Filarial (immunology), Humans, Immunoglobulin D, Immunoglobulin E (blood), Immunoglobulin G (biosynthesis), Immunoglobulin G (blood), Immunoglobulin Switch Region, Immunoglobulin gamma-Chains (genetics), Interleukin-13 (metabolism), Interleukin-4 (metabolism), Leukocytes, Mononuclear (immunology), Leukocytes, Mononuclear (metabolism), RNA, Messenger.
MESH :
- chemical , biosynthesis : Immunoglobulin G.
- chemical , blood : Immunoglobulin E, Immunoglobulin G.
- chemical , genetics : Immunoglobulin gamma-Chains.
- chemical , metabolism : Interleukin-13, Interleukin-4.
- chemical : Immunoglobulin D, RNA, Messenger.
- blood : Elephantiasis, Filarial.
- immunology : B-Lymphocytes, Elephantiasis, Filarial, Leukocytes, Mononuclear.
- metabolism : Leukocytes, Mononuclear.
- Adult, Animals, Humans, Immunoglobulin Switch Region.

Abstract

IgG4 and IgE isotypes contribute marginally to the pool of circulating antibodies in healthy individuals, but are elevated during atopic diseases and particularly upon helminth infections. To examine whether the high levels of these isotypes in circulation are reflected in a higher capacity of PBMC to produce IgG4 and IgE, we examined cells from patients infected with filarial nematodes that exhibit high levels of IgG4 and IgE. Indeed, IgG4 production by PBMC correlated strongly with plasma levels of IgG4 (r = 0.534, P = 0.002), but such correlation was not found for IgE. The replacement of CD19⁺ cells from PBMC by IgD⁺ cells abrogated the high capacity of PBMC to make IgG4. This indicates that an altered B cell compartment accounts for the high IgG4-producing capacity of the PBMC. The high production of IgG4 in vitro was not dependent on IL-4 and IL-13, as neutralizing antibodies to these cytokines did not inhibit IgG4. However, IgE release by PBMC was dependent on IL-4 and IL-13. Anti-filarial IgG4 was detected in culture supernatants from filarial patients and its production was independent of IL-4 and IL-13. These results demonstrate that in individuals with elevated IgG4, the B cell compartment in PBMC carries cells that are already committed to IgG4 production and are independent of IL-4 and IL-13.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1905099

DOI: 10.1046/j.1365-2249.1998.00732.x
PubMed: 9822284
PubMed Central: 1905099

Affiliations:

Links to Exploration step

PMC:1905099

Le document en format XML

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 production of IgG4 by peripheral blood mononuclear cells (PBMC): the contribution of committed B cells</title>
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 production of IgG4 by peripheral blood mononuclear cells (PBMC): the contribution of committed B cells</title>
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<term>Elephantiasis, Filarial (immunology)</term>
<term>Humans</term>
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<term>RNA, Messenger</term>
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<term>Chaines gamma des immunoglobulines (génétique)</term>
<term>Filariose lymphatique (immunologie)</term>
<term>Filariose lymphatique (sang)</term>
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<term>Immunoglobuline D</term>
<term>Immunoglobuline E (sang)</term>
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<term>Immunoglobuline G (sang)</term>
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<front><div type="abstract" xml:lang="en"><p>IgG4 and IgE isotypes contribute marginally to the pool of circulating antibodies in healthy individuals, but are elevated during atopic diseases and particularly upon helminth infections. To examine whether the high levels of these isotypes in circulation are reflected in a higher capacity of PBMC to produce IgG4 and IgE, we examined cells from patients infected with filarial nematodes that exhibit high levels of IgG4 and IgE. Indeed, IgG4 production by PBMC correlated strongly with plasma levels of IgG4 (<italic>r</italic>
 = 0.534, <italic>P</italic>
 = 0.002), but such correlation was not found for IgE. The replacement of CD19<sup>+</sup>
 cells from PBMC by IgD<sup>+</sup>
 cells abrogated the high capacity of PBMC to make IgG4. This indicates that an altered B cell compartment accounts for the high IgG4-producing capacity of the PBMC. The high production of IgG4 <italic>in vitro</italic>
 was not dependent on IL-4 and IL-13, as neutralizing antibodies to these cytokines did not inhibit IgG4. However, IgE release by PBMC was dependent on IL-4 and IL-13. Anti-filarial IgG4 was detected in culture supernatants from filarial patients and its production was independent of IL-4 and IL-13. These results demonstrate that in individuals with elevated IgG4, the B cell compartment in PBMC carries cells that are already committed to IgG4 production and are independent of IL-4 and IL-13.</p>
</div>
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In vitro production of IgG4 by peripheral blood mononuclear cells (PBMC): the contribution of committed B cells

In vitro production of IgG4 by peripheral blood mononuclear cells (PBMC): the contribution of committed B cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur le lymphœdème
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