Immunopurification and measurement of IgE in serum samples from bancroftian filariasis patients.
Identifieur interne : 00B696 ( Ncbi/Checkpoint ); précédent : 00B695; suivant : 00B697Immunopurification and measurement of IgE in serum samples from bancroftian filariasis patients.
Auteurs : S E Marley [États-Unis] ; P J Lammie ; M L EberhardSource :
- The Journal of parasitology [ 0022-3395 ] ; 1996.
Descripteurs français
- KwdFr :
- Adulte, Animaux, Anticorps antihelminthe (immunologie), Anticorps antihelminthe (isolement et purification), Anticorps antihelminthe (sang), Anticorps monoclonaux (immunologie), Chromatographie d'affinité, Femelle, Filariose lymphatique (immunologie), Humains, Immunoglobuline E (immunologie), Immunoglobuline E (isolement et purification), Immunoglobuline E (sang), Immunoglobuline G (sang), Maladie chronique, Mâle, Sérums immuns (immunologie), Test ELISA, Wuchereria bancrofti (immunologie).
- MESH :
- immunologie : Anticorps antihelminthe, Anticorps monoclonaux, Filariose lymphatique, Immunoglobuline E, Sérums immuns, Wuchereria bancrofti.
- isolement et purification : Anticorps antihelminthe, Immunoglobuline E.
- sang : Anticorps antihelminthe, Immunoglobuline E, Immunoglobuline G.
- Adulte, Animaux, Chromatographie d'affinité, Femelle, Humains, Maladie chronique, Mâle, Test ELISA.
English descriptors
- KwdEn :
- Adult, Animals, Antibodies, Helminth (blood), Antibodies, Helminth (immunology), Antibodies, Helminth (isolation & purification), Antibodies, Monoclonal (immunology), Chromatography, Affinity, Chronic Disease, Elephantiasis, Filarial (immunology), Enzyme-Linked Immunosorbent Assay, Female, Humans, Immune Sera (immunology), Immunoglobulin E (blood), Immunoglobulin E (immunology), Immunoglobulin E (isolation & purification), Immunoglobulin G (blood), Male, Wuchereria bancrofti (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth, Immunoglobulin E, Immunoglobulin G.
- chemical , immunology : Antibodies, Helminth, Antibodies, Monoclonal, Immune Sera, Immunoglobulin E.
- chemical , isolation & purification : Antibodies, Helminth, Immunoglobulin E.
- immunology : Elephantiasis, Filarial, Wuchereria bancrofti.
- Adult, Animals, Chromatography, Affinity, Chronic Disease, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male.
Abstract
Patent infection with Wuchereria bancrofti is associated with increased levels of filaria-specific IgG4 and IgE. In vitro quantification of filaria-specific IgE is hampered by its small proportion in serum relative to other isotypes and by potential competition with IgG4 for the same epitopes on parasite antigens. To determine if IgG4 or other isotypes inhibit the detection of parasite-specific IgE, total IgE was affinity purified prior to filaria-specific IgE enzyme-linked immunosorbent assay. Briefly, anti-human IgE mouse monoclonal antibody 6H10 was coupled to Affigel, and 50 microliters of patient serum was incubated on microcolumns for 16 hr. Total IgE was eluted with 25 mM triethylamine (pH 11.2) and levels of total and filaria-specific IgE and total IgG4 were assessed in the filtrates and eluates. Sera from 14 patients with W. bancrofti microfilaremia (Mf+) and 17 amicrofilaremic patients with chronic pathology (CP) were assayed. Filtrates and eluates were devoid of IgE and IgG4, respectively. The average yield of total IgE in the eluates was 70% (SEM = 6.5; range 21-100%) of that measured in the serum. Antifilarial IgE levels in column eluates were significantly higher in serum samples from CP patients than Mf+ patients. Antibody inhibition of IgE was assessed by comparing the levels of anti-filarial IgE detected in eluates and serum. Evidence for antibody-mediated inhibition of IgE detection was obtained with 2/2 samples from Indian tropical pulmonary eosinophilia patients, but only 2/14 and 4/17 Mf+ and CP patients, respectively.
PubMed: 8627493
Affiliations:
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sort="Marley, S E" uniqKey="Marley S" first="S E" last="Marley">S E Marley</name><affiliation wicri:level="1"><nlm:affiliation>Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341-3724</wicri:regionArea><wicri:noRegion>Georgia 30341-3724</wicri:noRegion></affiliation></author><author><name sortKey="Lammie, P J" sort="Lammie, P J" uniqKey="Lammie P" first="P J" last="Lammie">P J Lammie</name></author><author><name sortKey="Eberhard, M L" sort="Eberhard, M L" uniqKey="Eberhard M" first="M L" last="Eberhard">M L Eberhard</name></author></analytic><series><title level="j">The Journal of parasitology</title><idno type="ISSN">0022-3395</idno><imprint><date when="1996" 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(immunologie)</term><term>Anticorps antihelminthe (isolement et purification)</term><term>Anticorps antihelminthe (sang)</term><term>Anticorps monoclonaux (immunologie)</term><term>Chromatographie d'affinité</term><term>Femelle</term><term>Filariose lymphatique (immunologie)</term><term>Humains</term><term>Immunoglobuline E (immunologie)</term><term>Immunoglobuline E (isolement et purification)</term><term>Immunoglobuline E (sang)</term><term>Immunoglobuline G (sang)</term><term>Maladie chronique</term><term>Mâle</term><term>Sérums immuns (immunologie)</term><term>Test ELISA</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term><term>Immunoglobulin E</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antibodies, Monoclonal</term><term>Immune Sera</term><term>Immunoglobulin E</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Helminth</term><term>Immunoglobulin E</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Anticorps monoclonaux</term><term>Filariose lymphatique</term><term>Immunoglobuline E</term><term>Sérums immuns</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Immunoglobuline E</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Immunoglobuline E</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Animals</term><term>Chromatography, Affinity</term><term>Chronic Disease</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Female</term><term>Humans</term><term>Male</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Animaux</term><term>Chromatographie d'affinité</term><term>Femelle</term><term>Humains</term><term>Maladie chronique</term><term>Mâle</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Patent infection with Wuchereria bancrofti is associated with increased levels of filaria-specific IgG4 and IgE. In vitro quantification of filaria-specific IgE is hampered by its small proportion in serum relative to other isotypes and by potential competition with IgG4 for the same epitopes on parasite antigens. To determine if IgG4 or other isotypes inhibit the detection of parasite-specific IgE, total IgE was affinity purified prior to filaria-specific IgE enzyme-linked immunosorbent assay. Briefly, anti-human IgE mouse monoclonal antibody 6H10 was coupled to Affigel, and 50 microliters of patient serum was incubated on microcolumns for 16 hr. Total IgE was eluted with 25 mM triethylamine (pH 11.2) and levels of total and filaria-specific IgE and total IgG4 were assessed in the filtrates and eluates. Sera from 14 patients with W. bancrofti microfilaremia (Mf+) and 17 amicrofilaremic patients with chronic pathology (CP) were assayed. Filtrates and eluates were devoid of IgE and IgG4, respectively. The average yield of total IgE in the eluates was 70% (SEM = 6.5; range 21-100%) of that measured in the serum. Antifilarial IgE levels in column eluates were significantly higher in serum samples from CP patients than Mf+ patients. Antibody inhibition of IgE was assessed by comparing the levels of anti-filarial IgE detected in eluates and serum. Evidence for antibody-mediated inhibition of IgE detection was obtained with 2/2 samples from Indian tropical pulmonary eosinophilia patients, but only 2/14 and 4/17 Mf+ and CP patients, respectively.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Eberhard, M L" sort="Eberhard, M L" uniqKey="Eberhard M" first="M L" last="Eberhard">M L Eberhard</name><name sortKey="Lammie, P J" sort="Lammie, P J" uniqKey="Lammie P" first="P J" last="Lammie">P J Lammie</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Marley, S E" sort="Marley, S E" uniqKey="Marley S" first="S E" last="Marley">S E Marley</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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