Parasite antigens in sera and urine of patients with bancroftian and brugian filariasis detected by sandwich ELISA with monoclonal antibodies.
Identifieur interne : 009897 ( Ncbi/Checkpoint ); précédent : 009896; suivant : 009898Parasite antigens in sera and urine of patients with bancroftian and brugian filariasis detected by sandwich ELISA with monoclonal antibodies.
Auteurs : H J Zheng ; Z H Tao ; M V Reddy ; B C Harinath ; W F PiessensSource :
- The American journal of tropical medicine and hygiene [ 0002-9637 ] ; 1987.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Antigènes d'helminthe.
- immunologie : Brugia, Filariose lymphatique, Wuchereria, Wuchereria bancrofti.
- urine : Antigènes d'helminthe.
- Anticorps monoclonaux, Microfilaria, Test ELISA.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Helminth.
- chemical , urine : Antigens, Helminth.
- chemical : Antibodies, Monoclonal.
- immunology : Brugia, Elephantiasis, Filarial, Wuchereria, Wuchereria bancrofti.
- Enzyme-Linked Immunosorbent Assay, Microfilariae.
Abstract
We developed a sandwich enzyme-linked immunosorbent assay to detect circulating parasite antigens in human lymphatic filariasis. The assay utilizes a polyclonal rabbit antifilarial antiserum to capture, and a monoclonal antibody to identify, circulating parasite antigen. Using this assay, we found that greater than 95% of sera from microfilaremic donors with bancroftian or brugian filariasis, approximately 60% of sera from microfilaremic patients with hydroceles, chyluria, or elephantiasis, and 15%-20% of sera from asymptomatic residents of filariasis-endemic areas evidently contain filarial antigens. Antigen was also detected in the urine of some microfilaremic patients. Serum levels of antigen detected with one monoclonal antibody, ES34, correlated well with microfilarial density in night blood. In contrast, less than 5% of sera from residents of areas where lymphatic filariasis is not endemic reacted in the assay, even though approximately one-third of the donors whose sera were tested were known to be infected with intestinal nematodes. The assay was designed to be flexible enough to allow the parallel use of multiple monoclonal antibodies with different specificities and simple enough to be applicable in most areas where lymphatic filariasis is endemic.
PubMed: 3555138
Affiliations:
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pubmed:3555138Le document en format XML
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The assay utilizes a polyclonal rabbit antifilarial antiserum to capture, and a monoclonal antibody to identify, circulating parasite antigen. Using this assay, we found that greater than 95% of sera from microfilaremic donors with bancroftian or brugian filariasis, approximately 60% of sera from microfilaremic patients with hydroceles, chyluria, or elephantiasis, and 15%-20% of sera from asymptomatic residents of filariasis-endemic areas evidently contain filarial antigens. Antigen was also detected in the urine of some microfilaremic patients. Serum levels of antigen detected with one monoclonal antibody, ES34, correlated well with microfilarial density in night blood. In contrast, less than 5% of sera from residents of areas where lymphatic filariasis is not endemic reacted in the assay, even though approximately one-third of the donors whose sera were tested were known to be infected with intestinal nematodes. The assay was designed to be flexible enough to allow the parallel use of multiple monoclonal antibodies with different specificities and simple enough to be applicable in most areas where lymphatic filariasis is endemic.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Harinath, B C" sort="Harinath, B C" uniqKey="Harinath B" first="B C" last="Harinath">B C Harinath</name><name sortKey="Piessens, W F" sort="Piessens, W F" uniqKey="Piessens W" first="W F" last="Piessens">W F Piessens</name><name sortKey="Reddy, M V" sort="Reddy, M V" uniqKey="Reddy M" first="M V" last="Reddy">M V Reddy</name><name sortKey="Tao, Z H" sort="Tao, Z H" uniqKey="Tao Z" first="Z H" last="Tao">Z H Tao</name><name sortKey="Zheng, H J" sort="Zheng, H J" uniqKey="Zheng H" first="H J" last="Zheng">H J Zheng</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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