Wuchereria bancrofti: identification of parasitic acetylcholinesterase in microfilariae infected human serum.
Identifieur interne : 00D586 ( Main/Merge ); précédent : 00D585; suivant : 00D587Wuchereria bancrofti: identification of parasitic acetylcholinesterase in microfilariae infected human serum.
Auteurs : S. Misra [Inde] ; T M Mohapatra ; S. RathaurSource :
- Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) [ 0177-2392 ] ; 1993.
Descripteurs français
- KwdFr :
- Acetylcholinesterase (), Acetylcholinesterase (analyse), Acetylcholinesterase (immunologie), Acetylcholinesterase (sang), Acétyl-thiocholine (métabolisme), Animaux, Antigènes d'helminthe (analyse), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (sang), Butyryl-thiocholine (métabolisme), Butyrylcholine esterase (), Butyrylcholine esterase (immunologie), Butyrylcholine esterase (isolement et purification), Butyrylcholine esterase (sang), Complexe antigène-anticorps (), Filariose lymphatique (enzymologie), Filariose lymphatique (sang), Microfilaria (enzymologie), Microfilaria (immunologie), Physostigmine (pharmacologie), Relation dose-effet des médicaments, Spécificité du substrat, Wuchereria bancrofti (enzymologie), Wuchereria bancrofti (immunologie).
- MESH :
- analyse : Acetylcholinesterase, Antigènes d'helminthe.
- enzymologie : Filariose lymphatique, Microfilaria, Wuchereria bancrofti.
- immunologie : Acetylcholinesterase, Antigènes d'helminthe, Butyrylcholine esterase, Microfilaria, Wuchereria bancrofti.
- isolement et purification : Butyrylcholine esterase.
- métabolisme : Acétyl-thiocholine, Butyryl-thiocholine.
- pharmacologie : Physostigmine.
- sang : Acetylcholinesterase, Antigènes d'helminthe, Butyrylcholine esterase, Filariose lymphatique.
- Acetylcholinesterase, Animaux, Butyrylcholine esterase, Complexe antigène-anticorps, Relation dose-effet des médicaments, Spécificité du substrat.
English descriptors
- KwdEn :
- Acetylcholinesterase (analysis), Acetylcholinesterase (blood), Acetylcholinesterase (drug effects), Acetylcholinesterase (immunology), Acetylthiocholine (metabolism), Animals, Antigen-Antibody Complex (chemistry), Antigens, Helminth (analysis), Antigens, Helminth (blood), Antigens, Helminth (immunology), Butyrylcholinesterase (blood), Butyrylcholinesterase (drug effects), Butyrylcholinesterase (immunology), Butyrylcholinesterase (isolation & purification), Butyrylthiocholine (metabolism), Dose-Response Relationship, Drug, Elephantiasis, Filarial (blood), Elephantiasis, Filarial (enzymology), Microfilariae (enzymology), Microfilariae (immunology), Physostigmine (pharmacology), Substrate Specificity, Wuchereria bancrofti (enzymology), Wuchereria bancrofti (immunology).
- MESH :
- chemical , analysis : Acetylcholinesterase, Antigens, Helminth.
- chemical , blood : Acetylcholinesterase, Antigens, Helminth, Butyrylcholinesterase.
- chemical , chemistry : Antigen-Antibody Complex.
- chemical , drug effects : Acetylcholinesterase, Butyrylcholinesterase.
- chemical , immunology : Acetylcholinesterase, Antigens, Helminth, Butyrylcholinesterase.
- chemical , isolation & purification : Butyrylcholinesterase.
- chemical , metabolism : Acetylthiocholine, Butyrylthiocholine.
- blood : Elephantiasis, Filarial.
- enzymology : Elephantiasis, Filarial, Microfilariae, Wuchereria bancrofti.
- immunology : Microfilariae, Wuchereria bancrofti.
- chemical , pharmacology : Physostigmine.
- Animals, Dose-Response Relationship, Drug, Substrate Specificity.
Abstract
An antigen with cholinesterase activity was detected in the sera of patients infected with Wuchereria bancrofti. The asymptomatic microfilaremic sera showed 3 to 4 times more cholinesterase activity for acetylthiocholine (ATCh) as compared to sera of symptomatic amicrofilaremic, hookworm infected and endemic normals, whereas the activities for butyrylthiocholine (BTCh) did not significantly differ. The enzyme activities from both sources, namely from sera of microfilaremic cases and from endemic normals, were partially purified and according to substrate specificity for ATCh and BTCh as well as inhibition of the former activity by excess substrate classified as acetylcholinesterase (AChE; EC 3.1.1.7) and pseudocholinesterase (AChE; EC 3.1.1.8), respectively. The Km-value for ATCh of the cholinesterase from the microfilaremic sera was determined to be 0.87 mM. Eserine competitively inhibited the AChE activity; the inhibition constant was found to be 1.3 microM. The BChE from the normal sera had Km-values of 0.15 and 0.20 mM for BTCh and ATCh, respectively, and did not show significant inhibition by eserine. These and other dissimilarities suggest a difference in nature of the cholinesterases in microfilaremic and normal sera and propose that the former enzyme, a true acetylcholinesterase, originates from the parasite. Additional evidence for the origin of the AChE-activity from the parasite was provided by ELISA-studies; anti-Brugia malayi AChE antibodies confirmed antigenecity and cross reactivity of the AChE in infected sera, whereas the antibodies did not show any cross reactivity with the BChE in normal sera.
PubMed: 8367669
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<term>Acetylcholinesterase (blood)</term>
<term>Acetylcholinesterase (drug effects)</term>
<term>Acetylcholinesterase (immunology)</term>
<term>Acetylthiocholine (metabolism)</term>
<term>Animals</term>
<term>Antigen-Antibody Complex (chemistry)</term>
<term>Antigens, Helminth (analysis)</term>
<term>Antigens, Helminth (blood)</term>
<term>Antigens, Helminth (immunology)</term>
<term>Butyrylcholinesterase (blood)</term>
<term>Butyrylcholinesterase (drug effects)</term>
<term>Butyrylcholinesterase (immunology)</term>
<term>Butyrylcholinesterase (isolation & purification)</term>
<term>Butyrylthiocholine (metabolism)</term>
<term>Dose-Response Relationship, Drug</term>
<term>Elephantiasis, Filarial (blood)</term>
<term>Elephantiasis, Filarial (enzymology)</term>
<term>Microfilariae (enzymology)</term>
<term>Microfilariae (immunology)</term>
<term>Physostigmine (pharmacology)</term>
<term>Substrate Specificity</term>
<term>Wuchereria bancrofti (enzymology)</term>
<term>Wuchereria bancrofti (immunology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Acetylcholinesterase ()</term>
<term>Acetylcholinesterase (analyse)</term>
<term>Acetylcholinesterase (immunologie)</term>
<term>Acetylcholinesterase (sang)</term>
<term>Acétyl-thiocholine (métabolisme)</term>
<term>Animaux</term>
<term>Antigènes d'helminthe (analyse)</term>
<term>Antigènes d'helminthe (immunologie)</term>
<term>Antigènes d'helminthe (sang)</term>
<term>Butyryl-thiocholine (métabolisme)</term>
<term>Butyrylcholine esterase ()</term>
<term>Butyrylcholine esterase (immunologie)</term>
<term>Butyrylcholine esterase (isolement et purification)</term>
<term>Butyrylcholine esterase (sang)</term>
<term>Complexe antigène-anticorps ()</term>
<term>Filariose lymphatique (enzymologie)</term>
<term>Filariose lymphatique (sang)</term>
<term>Microfilaria (enzymologie)</term>
<term>Microfilaria (immunologie)</term>
<term>Physostigmine (pharmacologie)</term>
<term>Relation dose-effet des médicaments</term>
<term>Spécificité du substrat</term>
<term>Wuchereria bancrofti (enzymologie)</term>
<term>Wuchereria bancrofti (immunologie)</term>
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<term>Antigens, Helminth</term>
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<term>Antigens, Helminth</term>
<term>Butyrylcholinesterase</term>
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<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antigen-Antibody Complex</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="drug effects" xml:lang="en"><term>Acetylcholinesterase</term>
<term>Butyrylcholinesterase</term>
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<term>Antigens, Helminth</term>
<term>Butyrylcholinesterase</term>
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<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Butyrylcholinesterase</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acetylthiocholine</term>
<term>Butyrylthiocholine</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Acetylcholinesterase</term>
<term>Antigènes d'helminthe</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Elephantiasis, Filarial</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Filariose lymphatique</term>
<term>Microfilaria</term>
<term>Wuchereria bancrofti</term>
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<term>Microfilariae</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Acetylcholinesterase</term>
<term>Antigènes d'helminthe</term>
<term>Butyrylcholine esterase</term>
<term>Microfilaria</term>
<term>Wuchereria bancrofti</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Microfilariae</term>
<term>Wuchereria bancrofti</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Butyrylcholine esterase</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acétyl-thiocholine</term>
<term>Butyryl-thiocholine</term>
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<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Physostigmine</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Physostigmine</term>
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<term>Dose-Response Relationship, Drug</term>
<term>Substrate Specificity</term>
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<term>Animaux</term>
<term>Butyrylcholine esterase</term>
<term>Complexe antigène-anticorps</term>
<term>Relation dose-effet des médicaments</term>
<term>Spécificité du substrat</term>
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<front><div type="abstract" xml:lang="en">An antigen with cholinesterase activity was detected in the sera of patients infected with Wuchereria bancrofti. The asymptomatic microfilaremic sera showed 3 to 4 times more cholinesterase activity for acetylthiocholine (ATCh) as compared to sera of symptomatic amicrofilaremic, hookworm infected and endemic normals, whereas the activities for butyrylthiocholine (BTCh) did not significantly differ. The enzyme activities from both sources, namely from sera of microfilaremic cases and from endemic normals, were partially purified and according to substrate specificity for ATCh and BTCh as well as inhibition of the former activity by excess substrate classified as acetylcholinesterase (AChE; EC 3.1.1.7) and pseudocholinesterase (AChE; EC 3.1.1.8), respectively. The Km-value for ATCh of the cholinesterase from the microfilaremic sera was determined to be 0.87 mM. Eserine competitively inhibited the AChE activity; the inhibition constant was found to be 1.3 microM. The BChE from the normal sera had Km-values of 0.15 and 0.20 mM for BTCh and ATCh, respectively, and did not show significant inhibition by eserine. These and other dissimilarities suggest a difference in nature of the cholinesterases in microfilaremic and normal sera and propose that the former enzyme, a true acetylcholinesterase, originates from the parasite. Additional evidence for the origin of the AChE-activity from the parasite was provided by ELISA-studies; anti-Brugia malayi AChE antibodies confirmed antigenecity and cross reactivity of the AChE in infected sera, whereas the antibodies did not show any cross reactivity with the BChE in normal sera.</div>
</front>
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