Therapeutic lymphangiogenesis with human recombinant VEGF-C.
Identifieur interne : 009674 ( Main/Merge ); précédent : 009673; suivant : 009675Therapeutic lymphangiogenesis with human recombinant VEGF-C.
Auteurs : Andrzej Szuba [États-Unis] ; Mihaela Skobe ; Marika J. Karkkainen ; William S. Shin ; David P. Beynet ; Ned B. Rockson ; Noma Dakhil ; Stan Spilman ; Michael L. Goris ; H William Strauss ; Thomas Quertermous ; Kari Alitalo [Finlande] ; Stanley G. RocksonSource :
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology [ 1530-6860 ] ; 2002.
Descripteurs français
- KwdFr :
- Animaux, Derme (anatomopathologie), Facteur de croissance endothéliale vasculaire de type C, Facteurs de croissance endothéliale (génétique), Facteurs de croissance endothéliale (usage thérapeutique), Humains, Immunohistochimie, Lapins, Lymphoedème (anatomopathologie), Lymphoedème (physiopathologie), Lymphoedème (traitement médicamenteux), Maladie chronique, Peau (anatomopathologie), Protéines recombinantes (usage thérapeutique), Système lymphatique (), Système lymphatique (croissance et développement), Système lymphatique (physiopathologie).
- MESH :
- anatomopathologie : Derme, Lymphoedème, Peau.
- croissance et développement : Système lymphatique.
- génétique : Facteurs de croissance endothéliale.
- physiopathologie : Lymphoedème, Système lymphatique.
- traitement médicamenteux : Lymphoedème.
- usage thérapeutique : Facteurs de croissance endothéliale, Protéines recombinantes.
- Animaux, Facteur de croissance endothéliale vasculaire de type C, Humains, Immunohistochimie, Lapins, Maladie chronique, Système lymphatique.
English descriptors
- KwdEn :
- Animals, Chronic Disease, Dermis (pathology), Endothelial Growth Factors (genetics), Endothelial Growth Factors (therapeutic use), Humans, Immunohistochemistry, Lymphatic System (drug effects), Lymphatic System (growth & development), Lymphatic System (physiopathology), Lymphedema (drug therapy), Lymphedema (pathology), Lymphedema (physiopathology), Rabbits, Recombinant Proteins (therapeutic use), Skin (pathology), Vascular Endothelial Growth Factor C.
- MESH :
- chemical , genetics : Endothelial Growth Factors.
- drug effects : Lymphatic System.
- drug therapy : Lymphedema.
- growth & development : Lymphatic System.
- pathology : Dermis, Lymphedema, Skin.
- physiopathology : Lymphatic System, Lymphedema.
- chemical , therapeutic use : Endothelial Growth Factors, Recombinant Proteins.
- Animals, Chronic Disease, Humans, Immunohistochemistry, Rabbits, Vascular Endothelial Growth Factor C.
Abstract
Chronic regional impairments of the lymphatic circulation often lead to striking architectural abnormalities in the lymphedematous tissues. Lymphedema is a common, disabling disease that currently lacks a cure. Vascular endothelial growth factors C and D mediate lymphangiogenesis through the VEGFR-3 receptor on lymphatic endothelia. The purpose of this study was to investigate the therapeutic potential for lymphangiogenesis with VEGF-C. We developed a rabbit ear model to simulate human chronic postsurgical lymphatic insufficiency. Successful, sustained surgical ablation of the ear lymphatics was confirmed by water displacement volumetry. After complete healing, the experimental animals (n=8) received a single, s.c. 100 microg dose of VEGF-C in the operated ear; controls (n=8) received normal saline. Radionuclide lymphoscintigraphy was performed to quantitate lymphatic function. Immunohistochemistry (IHC) was performed 7-8 days following treatment. After VEGF-C, there was a quantifiable amelioration of lymphatic function. IHC confirmed a significant increase in lymphatic vascularity, along with reversal of the intense tissue hypercellularity of untreated lymphedema. This study confirms the capacity of a single dose of VEGF-C to induce therapeutic lymphangiogenesis in acquired lymphedema. In addition to improving lymphatic function and vascularity, VEGF-C can apparently reverse the abnormalities in tissue architecture that accompany chronic lymphatic insufficiency.
DOI: 10.1096/fj.02-0401fje
PubMed: 12397087
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pubmed:12397087Le document en format XML
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Rockson</name></author></analytic><series><title level="j">FASEB journal : official publication of the Federation of American Societies for Experimental Biology</title><idno type="eISSN">1530-6860</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chronic Disease</term><term>Dermis (pathology)</term><term>Endothelial Growth Factors (genetics)</term><term>Endothelial Growth Factors (therapeutic use)</term><term>Humans</term><term>Immunohistochemistry</term><term>Lymphatic System (drug effects)</term><term>Lymphatic System (growth & development)</term><term>Lymphatic System (physiopathology)</term><term>Lymphedema (drug therapy)</term><term>Lymphedema (pathology)</term><term>Lymphedema (physiopathology)</term><term>Rabbits</term><term>Recombinant Proteins (therapeutic use)</term><term>Skin (pathology)</term><term>Vascular Endothelial Growth Factor C</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Derme (anatomopathologie)</term><term>Facteur de croissance endothéliale vasculaire de type C</term><term>Facteurs de croissance endothéliale (génétique)</term><term>Facteurs de croissance endothéliale (usage thérapeutique)</term><term>Humains</term><term>Immunohistochimie</term><term>Lapins</term><term>Lymphoedème (anatomopathologie)</term><term>Lymphoedème (physiopathologie)</term><term>Lymphoedème (traitement médicamenteux)</term><term>Maladie chronique</term><term>Peau (anatomopathologie)</term><term>Protéines recombinantes (usage thérapeutique)</term><term>Système lymphatique ()</term><term>Système lymphatique (croissance et développement)</term><term>Système lymphatique (physiopathologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Endothelial Growth Factors</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Derme</term><term>Lymphoedème</term><term>Peau</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Système lymphatique</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Lymphatic System</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Lymphedema</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Lymphatic System</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Facteurs de croissance endothéliale</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Dermis</term><term>Lymphedema</term><term>Skin</term></keywords><keywords scheme="MESH" qualifier="physiopathologie" xml:lang="fr"><term>Lymphoedème</term><term>Système lymphatique</term></keywords><keywords scheme="MESH" qualifier="physiopathology" xml:lang="en"><term>Lymphatic System</term><term>Lymphedema</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Endothelial Growth Factors</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Lymphoedème</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Facteurs de croissance endothéliale</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chronic Disease</term><term>Humans</term><term>Immunohistochemistry</term><term>Rabbits</term><term>Vascular Endothelial Growth Factor C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Facteur de croissance endothéliale vasculaire de type C</term><term>Humains</term><term>Immunohistochimie</term><term>Lapins</term><term>Maladie chronique</term><term>Système lymphatique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chronic regional impairments of the lymphatic circulation often lead to striking architectural abnormalities in the lymphedematous tissues. Lymphedema is a common, disabling disease that currently lacks a cure. Vascular endothelial growth factors C and D mediate lymphangiogenesis through the VEGFR-3 receptor on lymphatic endothelia. The purpose of this study was to investigate the therapeutic potential for lymphangiogenesis with VEGF-C. We developed a rabbit ear model to simulate human chronic postsurgical lymphatic insufficiency. Successful, sustained surgical ablation of the ear lymphatics was confirmed by water displacement volumetry. After complete healing, the experimental animals (n=8) received a single, s.c. 100 microg dose of VEGF-C in the operated ear; controls (n=8) received normal saline. Radionuclide lymphoscintigraphy was performed to quantitate lymphatic function. Immunohistochemistry (IHC) was performed 7-8 days following treatment. After VEGF-C, there was a quantifiable amelioration of lymphatic function. IHC confirmed a significant increase in lymphatic vascularity, along with reversal of the intense tissue hypercellularity of untreated lymphedema. This study confirms the capacity of a single dose of VEGF-C to induce therapeutic lymphangiogenesis in acquired lymphedema. In addition to improving lymphatic function and vascularity, VEGF-C can apparently reverse the abnormalities in tissue architecture that accompany chronic lymphatic insufficiency.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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