Abnormal lymphangiogenesis in idiopathic pulmonary fibrosis with insights into cellular and molecular mechanisms
Identifieur interne : 006494 ( Main/Merge ); précédent : 006493; suivant : 006495Abnormal lymphangiogenesis in idiopathic pulmonary fibrosis with insights into cellular and molecular mechanisms
Auteurs : Souheil El-Chemaly ; Daniela Malide ; Enrique Zudaire [États-Unis] ; Yoshihiko Ikeda ; Benjamin A. Weinberg ; Gustavo Pacheco-Rodriguez ; Ivan O. Rosas ; Marta Aparicio [États-Unis] ; Ping Ren ; Sandra D. Macdonald ; Hai-Ping Wu ; Steven D. Nathan [États-Unis] ; Frank Cuttitta [États-Unis] ; J. Philip Mccoy [États-Unis] ; Bernadette R. Gochuico [États-Unis] ; Joel MossSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2009.
Abstract
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, debilitating respiratory disease whose pathogenesis is poorly understood. In IPF, the lung parenchyma undergoes extensive remodeling. We hypothesized that lymphangiogenesis is part of lung remodeling and sought to characterize pathways leading to lymphangiogenesis in IPF. We found that the diameter of lymphatic vessels in alveolar spaces in IPF lung tissue correlated with disease severity, suggesting that the alveolar microenvironment plays a role in the lymphangiogenic process. In bronchoalveolar lavage fluid (BALF) from subjects with IPF, we found short-fragment hyaluronic acid, which induced migration and proliferation of lymphatic endothelial cells (LECs), processes required for lymphatic vessel formation. To determine the origin of LECs in IPF, we isolated macrophages from the alveolar spaces; CD11b+ macrophages from subjects with IPF, but not those from healthy volunteers, formed lymphatic-like vessels in vitro. Our findings demonstrate that in the alveolar microenvironment of IPF, soluble factors such as short-fragment hyaluronic acid and cells such as CD11b+ macrophages contribute to lymphangiogenesis. These results improve our understanding of lymphangiogenesis and tissue remodeling in IPF and perhaps other fibrotic diseases as well.
Url:
DOI: 10.1073/pnas.0813368106
PubMed: 19237567
PubMed Central: 2646625
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 003162
- to stream Pmc, to step Curation: 003161
- to stream Pmc, to step Checkpoint: 003829
- to stream Ncbi, to step Merge: 003250
- to stream Ncbi, to step Curation: 003250
- to stream Ncbi, to step Checkpoint: 003250
Links to Exploration step
PMC:2646625Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Abnormal lymphangiogenesis in idiopathic pulmonary fibrosis with insights into cellular and molecular mechanisms</title><author><name sortKey="El Chemaly, Souheil" sort="El Chemaly, Souheil" uniqKey="El Chemaly S" first="Souheil" last="El-Chemaly">Souheil El-Chemaly</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Malide, Daniela" sort="Malide, Daniela" uniqKey="Malide D" first="Daniela" last="Malide">Daniela Malide</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Light Microscopy Core Facility</nlm:aff></affiliation></author><author><name sortKey="Zudaire, Enrique" sort="Zudaire, Enrique" uniqKey="Zudaire E" first="Enrique" last="Zudaire">Enrique Zudaire</name><affiliation wicri:level="2"><nlm:aff id="aff4">Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg, MD 20877;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg</wicri:cityArea></affiliation></author><author><name sortKey="Ikeda, Yoshihiko" sort="Ikeda, Yoshihiko" uniqKey="Ikeda Y" first="Yoshihiko" last="Ikeda">Yoshihiko Ikeda</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Weinberg, Benjamin A" sort="Weinberg, Benjamin A" uniqKey="Weinberg B" first="Benjamin A." last="Weinberg">Benjamin A. Weinberg</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Light Microscopy Core Facility</nlm:aff></affiliation></author><author><name sortKey="Pacheco Rodriguez, Gustavo" sort="Pacheco Rodriguez, Gustavo" uniqKey="Pacheco Rodriguez G" first="Gustavo" last="Pacheco-Rodriguez">Gustavo Pacheco-Rodriguez</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Rosas, Ivan O" sort="Rosas, Ivan O" uniqKey="Rosas I" first="Ivan O." last="Rosas">Ivan O. Rosas</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Aparicio, Marta" sort="Aparicio, Marta" uniqKey="Aparicio M" first="Marta" last="Aparicio">Marta Aparicio</name><affiliation wicri:level="2"><nlm:aff id="aff4">Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg, MD 20877;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg</wicri:cityArea></affiliation></author><author><name sortKey="Ren, Ping" sort="Ren, Ping" uniqKey="Ren P" first="Ping" last="Ren">Ping Ren</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Macdonald, Sandra D" sort="Macdonald, Sandra D" uniqKey="Macdonald S" first="Sandra D." last="Macdonald">Sandra D. Macdonald</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Wu, Hai Ping" sort="Wu, Hai Ping" uniqKey="Wu H" first="Hai-Ping" last="Wu">Hai-Ping Wu</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Nathan, Steven D" sort="Nathan, Steven D" uniqKey="Nathan S" first="Steven D." last="Nathan">Steven D. Nathan</name><affiliation wicri:level="2"><nlm:aff wicri:cut="; and" id="aff5">Advanced Lung Disease and Transplant Program, Inova Heart & Vascular Institute, Inova Fairfax Hospital, Falls Church, VA 22042</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Virginie</region></placeName><wicri:cityArea>Advanced Lung Disease and Transplant Program, Inova Heart & Vascular Institute, Inova Fairfax Hospital, Falls Church</wicri:cityArea></affiliation></author><author><name sortKey="Cuttitta, Frank" sort="Cuttitta, Frank" uniqKey="Cuttitta F" first="Frank" last="Cuttitta">Frank Cuttitta</name><affiliation wicri:level="2"><nlm:aff id="aff4">Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg, MD 20877;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg</wicri:cityArea></affiliation></author><author><name sortKey="Mccoy, J Philip" sort="Mccoy, J Philip" uniqKey="Mccoy J" first="J. Philip" last="Mccoy">J. Philip Mccoy</name><affiliation wicri:level="2"><nlm:aff id="aff3">Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Gochuico, Bernadette R" sort="Gochuico, Bernadette R" uniqKey="Gochuico B" first="Bernadette R." last="Gochuico">Bernadette R. Gochuico</name><affiliation wicri:level="2"><nlm:aff id="aff6">Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Moss, Joel" sort="Moss, Joel" uniqKey="Moss J" first="Joel" last="Moss">Joel Moss</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19237567</idno><idno type="pmc">2646625</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2646625</idno><idno type="RBID">PMC:2646625</idno><idno type="doi">10.1073/pnas.0813368106</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">003162</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">003162</idno><idno type="wicri:Area/Pmc/Curation">003161</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">003161</idno><idno type="wicri:Area/Pmc/Checkpoint">003829</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">003829</idno><idno type="wicri:Area/Ncbi/Merge">003250</idno><idno type="wicri:Area/Ncbi/Curation">003250</idno><idno type="wicri:Area/Ncbi/Checkpoint">003250</idno><idno type="wicri:doubleKey">0027-8424:2009:El Chemaly S:abnormal:lymphangiogenesis:in</idno><idno type="wicri:Area/Main/Merge">006494</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Abnormal lymphangiogenesis in idiopathic pulmonary fibrosis with insights into cellular and molecular mechanisms</title><author><name sortKey="El Chemaly, Souheil" sort="El Chemaly, Souheil" uniqKey="El Chemaly S" first="Souheil" last="El-Chemaly">Souheil El-Chemaly</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Malide, Daniela" sort="Malide, Daniela" uniqKey="Malide D" first="Daniela" last="Malide">Daniela Malide</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Light Microscopy Core Facility</nlm:aff></affiliation></author><author><name sortKey="Zudaire, Enrique" sort="Zudaire, Enrique" uniqKey="Zudaire E" first="Enrique" last="Zudaire">Enrique Zudaire</name><affiliation wicri:level="2"><nlm:aff id="aff4">Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg, MD 20877;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg</wicri:cityArea></affiliation></author><author><name sortKey="Ikeda, Yoshihiko" sort="Ikeda, Yoshihiko" uniqKey="Ikeda Y" first="Yoshihiko" last="Ikeda">Yoshihiko Ikeda</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Weinberg, Benjamin A" sort="Weinberg, Benjamin A" uniqKey="Weinberg B" first="Benjamin A." last="Weinberg">Benjamin A. Weinberg</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Light Microscopy Core Facility</nlm:aff></affiliation></author><author><name sortKey="Pacheco Rodriguez, Gustavo" sort="Pacheco Rodriguez, Gustavo" uniqKey="Pacheco Rodriguez G" first="Gustavo" last="Pacheco-Rodriguez">Gustavo Pacheco-Rodriguez</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Rosas, Ivan O" sort="Rosas, Ivan O" uniqKey="Rosas I" first="Ivan O." last="Rosas">Ivan O. Rosas</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Aparicio, Marta" sort="Aparicio, Marta" uniqKey="Aparicio M" first="Marta" last="Aparicio">Marta Aparicio</name><affiliation wicri:level="2"><nlm:aff id="aff4">Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg, MD 20877;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg</wicri:cityArea></affiliation></author><author><name sortKey="Ren, Ping" sort="Ren, Ping" uniqKey="Ren P" first="Ping" last="Ren">Ping Ren</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Macdonald, Sandra D" sort="Macdonald, Sandra D" uniqKey="Macdonald S" first="Sandra D." last="Macdonald">Sandra D. Macdonald</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Wu, Hai Ping" sort="Wu, Hai Ping" uniqKey="Wu H" first="Hai-Ping" last="Wu">Hai-Ping Wu</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author><author><name sortKey="Nathan, Steven D" sort="Nathan, Steven D" uniqKey="Nathan S" first="Steven D." last="Nathan">Steven D. Nathan</name><affiliation wicri:level="2"><nlm:aff wicri:cut="; and" id="aff5">Advanced Lung Disease and Transplant Program, Inova Heart & Vascular Institute, Inova Fairfax Hospital, Falls Church, VA 22042</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Virginie</region></placeName><wicri:cityArea>Advanced Lung Disease and Transplant Program, Inova Heart & Vascular Institute, Inova Fairfax Hospital, Falls Church</wicri:cityArea></affiliation></author><author><name sortKey="Cuttitta, Frank" sort="Cuttitta, Frank" uniqKey="Cuttitta F" first="Frank" last="Cuttitta">Frank Cuttitta</name><affiliation wicri:level="2"><nlm:aff id="aff4">Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg, MD 20877;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Angiogenesis Core Facility, National Cancer Institute, National Institutes of Health, Gaithersburg</wicri:cityArea></affiliation></author><author><name sortKey="Mccoy, J Philip" sort="Mccoy, J Philip" uniqKey="Mccoy J" first="J. Philip" last="Mccoy">J. Philip Mccoy</name><affiliation wicri:level="2"><nlm:aff id="aff3">Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892;</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Flow Cytometry Core Facility, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Gochuico, Bernadette R" sort="Gochuico, Bernadette R" uniqKey="Gochuico B" first="Bernadette R." last="Gochuico">Bernadette R. Gochuico</name><affiliation wicri:level="2"><nlm:aff id="aff6">Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda</wicri:cityArea></affiliation></author><author><name sortKey="Moss, Joel" sort="Moss, Joel" uniqKey="Moss J" first="Joel" last="Moss">Joel Moss</name><affiliation><nlm:aff id="aff1">Translational Medicine Branch,</nlm:aff></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><idno type="eISSN">1091-6490</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive, debilitating respiratory disease whose pathogenesis is poorly understood. In IPF, the lung parenchyma undergoes extensive remodeling. We hypothesized that lymphangiogenesis is part of lung remodeling and sought to characterize pathways leading to lymphangiogenesis in IPF. We found that the diameter of lymphatic vessels in alveolar spaces in IPF lung tissue correlated with disease severity, suggesting that the alveolar microenvironment plays a role in the lymphangiogenic process. In bronchoalveolar lavage fluid (BALF) from subjects with IPF, we found short-fragment hyaluronic acid, which induced migration and proliferation of lymphatic endothelial cells (LECs), processes required for lymphatic vessel formation. To determine the origin of LECs in IPF, we isolated macrophages from the alveolar spaces; CD11b<sup>+</sup> macrophages from subjects with IPF, but not those from healthy volunteers, formed lymphatic-like vessels in vitro. Our findings demonstrate that in the alveolar microenvironment of IPF, soluble factors such as short-fragment hyaluronic acid and cells such as CD11b<sup>+</sup> macrophages contribute to lymphangiogenesis. These results improve our understanding of lymphangiogenesis and tissue remodeling in IPF and perhaps other fibrotic diseases as well.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006494 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 006494 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Merge
|type= RBID
|clé= PMC:2646625
|texte= Abnormal lymphangiogenesis in idiopathic pulmonary fibrosis with insights into cellular and molecular mechanisms
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:19237567" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |