Effect of low-energy extracorporeal shock wave on vascular regeneration after spinal cord injury and the recovery of motor function
Identifieur interne : 001152 ( Main/Merge ); précédent : 001151; suivant : 001153Effect of low-energy extracorporeal shock wave on vascular regeneration after spinal cord injury and the recovery of motor function
Auteurs : Lei Wang [République populaire de Chine] ; Yuquan Jiang [République populaire de Chine] ; Zheng Jiang [République populaire de Chine] ; Lizhang Han [République populaire de Chine]Source :
- Neuropsychiatric Disease and Treatment [ 1176-6328 ] ; 2016.
Abstract
Latest studies show that low-energy extracorporeal shock wave therapy (ESWT) can upregulate levels of vascular endothelial growth factor (VEGF). VEGF can ease nervous tissue harm after spinal cord injury (SCI). This study aims to explore whether low-energy ESWT can promote expression of VEGF, protect nervous tissue after SCI, and improve motor function.
Ninety adult female rats were divided into the following groups: Group A (simple laminectomy), Group B (laminectomy and low-energy ESWT), Group C (spinal cord injury), and Group D (spinal cord injury and low-energy ESWT). Impinger was used to cause thoracic spinal cord injury. Low-energy ESWT was applied as treatment after injury three times a week, for 3 weeks. After SCI, the Basso, Beattie, and Bresnahan (BBB) scale was used to evaluate motor function over a period of 42 days at different time points. Hematoxylin and eosin (HE) staining was used to evaluate nerve tissue injury. Neuronal nuclear antigen (NeuN) staining was also used to evaluate loss of neurons. Polymerase chain reaction was used to detect messenger RNA (mRNA) expression of VEGF and its receptor fms-like tyrosine kinase 1 (Flt-1). Immunostaining was used to evaluate VEGF protein expression level in myeloid tissue.
BBB scores of Groups A and B showed no significant result related to dyskinesia. HE and NeuN staining indicated that only using low-energy ESWT could not cause damage of nervous tissue in Group B. Recovery of motor function at 7, 35, and 42 days after SCI in Group D was better than that in Group C (
Low-energy ESWT promotes expression of VEGF, decreases secondary damage of nerve tissue, and improves recovery of motor function. It can be regarded as one mode of clinical routine adjunctive therapy for spinal injury.
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DOI: 10.2147/NDT.S82864
PubMed: 27621630
PubMed Central: 5012600
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China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Zheng" sort="Jiang, Zheng" uniqKey="Jiang Z" first="Zheng" last="Jiang">Zheng Jiang</name><affiliation wicri:level="1"><nlm:aff id="af1-ndt-12-2189">Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Han, Lizhang" sort="Han, Lizhang" uniqKey="Han L" first="Lizhang" last="Han">Lizhang Han</name><affiliation wicri:level="1"><nlm:aff id="af1-ndt-12-2189">Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">27621630</idno><idno type="pmc">5012600</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5012600</idno><idno type="RBID">PMC:5012600</idno><idno type="doi">10.2147/NDT.S82864</idno><date when="2016">2016</date><idno type="wicri:Area/Pmc/Corpus">004225</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">004225</idno><idno type="wicri:Area/Pmc/Curation">004224</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">004224</idno><idno type="wicri:Area/Pmc/Checkpoint">000A25</idno><idno type="wicri:explorRef" wicri:stream="Pmc" 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Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Yuquan" sort="Jiang, Yuquan" uniqKey="Jiang Y" first="Yuquan" last="Jiang">Yuquan Jiang</name><affiliation wicri:level="1"><nlm:aff id="af1-ndt-12-2189">Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Jiang, Zheng" sort="Jiang, Zheng" uniqKey="Jiang Z" first="Zheng" last="Jiang">Zheng Jiang</name><affiliation wicri:level="1"><nlm:aff id="af1-ndt-12-2189">Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author><author><name sortKey="Han, Lizhang" sort="Han, Lizhang" uniqKey="Han L" first="Lizhang" last="Han">Lizhang Han</name><affiliation wicri:level="1"><nlm:aff id="af1-ndt-12-2189">Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan, People’s Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Neurosurgery, Qilu Hospital of Shandong University, Jinan</wicri:regionArea><wicri:noRegion>Jinan</wicri:noRegion></affiliation></author></analytic><series><title level="j">Neuropsychiatric Disease and Treatment</title><idno type="ISSN">1176-6328</idno><idno type="eISSN">1178-2021</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Latest studies show that low-energy extracorporeal shock wave therapy (ESWT) can upregulate levels of vascular endothelial growth factor (VEGF). VEGF can ease nervous tissue harm after spinal cord injury (SCI). This study aims to explore whether low-energy ESWT can promote expression of VEGF, protect nervous tissue after SCI, and improve motor function.</p></sec><sec><title>Methods</title><p>Ninety adult female rats were divided into the following groups: Group A (simple laminectomy), Group B (laminectomy and low-energy ESWT), Group C (spinal cord injury), and Group D (spinal cord injury and low-energy ESWT). Impinger was used to cause thoracic spinal cord injury. Low-energy ESWT was applied as treatment after injury three times a week, for 3 weeks. After SCI, the Basso, Beattie, and Bresnahan (BBB) scale was used to evaluate motor function over a period of 42 days at different time points. Hematoxylin and eosin (HE) staining was used to evaluate nerve tissue injury. Neuronal nuclear antigen (NeuN) staining was also used to evaluate loss of neurons. Polymerase chain reaction was used to detect messenger RNA (mRNA) expression of VEGF and its receptor fms-like tyrosine kinase 1 (Flt-1). Immunostaining was used to evaluate VEGF protein expression level in myeloid tissue.</p></sec><sec><title>Results</title><p>BBB scores of Groups A and B showed no significant result related to dyskinesia. HE and NeuN staining indicated that only using low-energy ESWT could not cause damage of nervous tissue in Group B. Recovery of motor function at 7, 35, and 42 days after SCI in Group D was better than that in Group C (<italic>P</italic><0.05). Compared with Group C, number of NeuN-positive cells at 42 days after SCI increased significantly (<italic>P</italic><0.05). The mRNA levels of VEGF and Flt-1 and VEGF expression at 7 days after SCI in Group D were significantly higher than those in Group C (<italic>P</italic><0.05).</p></sec><sec><title>Conclusion</title><p>Low-energy ESWT promotes expression of VEGF, decreases secondary damage of nerve tissue, and improves recovery of motor function. 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Lee</name></author><author><name sortKey="Huang, Hy" uniqKey="Huang H">HY Huang</name></author><author><name sortKey="Yang, Yl" uniqKey="Yang Y">YL Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lacroix, S" uniqKey="Lacroix S">S Lacroix</name></author><author><name sortKey="Hamilton, Lk" uniqKey="Hamilton L">LK Hamilton</name></author><author><name sortKey="Vaugeois, A" uniqKey="Vaugeois A">A Vaugeois</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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