In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis.
Identifieur interne : 000F79 ( Main/Merge ); précédent : 000F78; suivant : 000F80In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis.
Auteurs : Mohd Saeed ; Mohd Adnan ; Saif Khan ; Eyad Al-Shammari ; Huma MustafaSource :
- Acta parasitologica [ 1896-1851 ] ; 2016.
Descripteurs français
- KwdFr :
- ADN des helminthes (génétique), Amorces ADN (génétique), Animaux, Biologie informatique, Bovins, Filarioidea (génétique), Filarioidea (isolement et purification), Filariose lymphatique (diagnostic), Humains, Réaction de polymérisation en chaîne (), Séquence conservée, Techniques de diagnostic moléculaire ().
- MESH :
- diagnostic : Filariose lymphatique.
- génétique : ADN des helminthes, Amorces ADN, Filarioidea.
- isolement et purification : Filarioidea.
- Animaux, Biologie informatique, Bovins, Humains, Réaction de polymérisation en chaîne, Séquence conservée, Techniques de diagnostic moléculaire.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA Primers, DNA, Helminth.
- diagnosis : Elephantiasis, Filarial.
- genetics : Filarioidea.
- isolation & purification : Filarioidea.
- methods : Molecular Diagnostic Techniques, Polymerase Chain Reaction.
- Animals, Cattle, Computational Biology, Conserved Sequence, Humans.
Abstract
Lymphatic filariasis (LF) is a chronic disease and is caused by the parasites Wuchereria bancrofti (W. bancrofti), Brugia malayi (B. malayi) and Brugia timori (B. timori). In the present study, Setaria cervi (S. cervi), a bovine filarial parasite has been used. Previously, it has been reported that the S. cervi shares some common proteins and antigenic determinants with that of human filarial parasite. The larval stages of filarial species usually cannot be identified by classical morphology. Hence, molecular characterization allows the identification of the parasites throughout all their developmental stages. The genomic DNA of S. cervi adult were isolated and estimated spectrophotometrically for the quantitative presence of DNA content. Screening of DNA sequences from filarial DNA GenBank and Expressed Sequence Tags (EST's) were performed for homologous sequences and then multiple sequence alignment was executed. The conserved sequences from multiple sequence alignment were used for In Silico primer designing. The successfully designed primers were used further in PCR amplifications. Therefore, in search of a promising diagnostic tool few genes were identified to be conserved in the human and bovine filariasis and these novel primers deigned may help to develop a promising diagnostic tool for identification of lymphatic filariasis.
DOI: 10.1515/ap-2016-0015
PubMed: 26751881
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000A61
- to stream PubMed, to step Curation: 000A61
- to stream PubMed, to step Checkpoint: 000A61
- to stream Ncbi, to step Merge: 007D34
- to stream Ncbi, to step Curation: 007D34
- to stream Ncbi, to step Checkpoint: 007D34
Links to Exploration step
pubmed:26751881Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis.</title><author><name sortKey="Saeed, Mohd" sort="Saeed, Mohd" uniqKey="Saeed M" first="Mohd" last="Saeed">Mohd Saeed</name></author><author><name sortKey="Adnan, Mohd" sort="Adnan, Mohd" uniqKey="Adnan M" first="Mohd" last="Adnan">Mohd Adnan</name></author><author><name sortKey="Khan, Saif" sort="Khan, Saif" uniqKey="Khan S" first="Saif" last="Khan">Saif Khan</name></author><author><name sortKey="Al Shammari, Eyad" sort="Al Shammari, Eyad" uniqKey="Al Shammari E" first="Eyad" last="Al-Shammari">Eyad Al-Shammari</name></author><author><name sortKey="Mustafa, Huma" sort="Mustafa, Huma" uniqKey="Mustafa H" first="Huma" last="Mustafa">Huma Mustafa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26751881</idno><idno type="pmid">26751881</idno><idno type="doi">10.1515/ap-2016-0015</idno><idno type="wicri:Area/PubMed/Corpus">000A61</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000A61</idno><idno type="wicri:Area/PubMed/Curation">000A61</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000A61</idno><idno type="wicri:Area/PubMed/Checkpoint">000A61</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000A61</idno><idno type="wicri:Area/Ncbi/Merge">007D34</idno><idno type="wicri:Area/Ncbi/Curation">007D34</idno><idno type="wicri:Area/Ncbi/Checkpoint">007D34</idno><idno type="wicri:Area/Main/Merge">000F79</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis.</title><author><name sortKey="Saeed, Mohd" sort="Saeed, Mohd" uniqKey="Saeed M" first="Mohd" last="Saeed">Mohd Saeed</name></author><author><name sortKey="Adnan, Mohd" sort="Adnan, Mohd" uniqKey="Adnan M" first="Mohd" last="Adnan">Mohd Adnan</name></author><author><name sortKey="Khan, Saif" sort="Khan, Saif" uniqKey="Khan S" first="Saif" last="Khan">Saif Khan</name></author><author><name sortKey="Al Shammari, Eyad" sort="Al Shammari, Eyad" uniqKey="Al Shammari E" first="Eyad" last="Al-Shammari">Eyad Al-Shammari</name></author><author><name sortKey="Mustafa, Huma" sort="Mustafa, Huma" uniqKey="Mustafa H" first="Huma" last="Mustafa">Huma Mustafa</name></author></analytic><series><title level="j">Acta parasitologica</title><idno type="eISSN">1896-1851</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Computational Biology</term><term>Conserved Sequence</term><term>DNA Primers (genetics)</term><term>DNA, Helminth (genetics)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Filarioidea (genetics)</term><term>Filarioidea (isolation & purification)</term><term>Humans</term><term>Molecular Diagnostic Techniques (methods)</term><term>Polymerase Chain Reaction (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des helminthes (génétique)</term><term>Amorces ADN (génétique)</term><term>Animaux</term><term>Biologie informatique</term><term>Bovins</term><term>Filarioidea (génétique)</term><term>Filarioidea (isolement et purification)</term><term>Filariose lymphatique (diagnostic)</term><term>Humains</term><term>Réaction de polymérisation en chaîne ()</term><term>Séquence conservée</term><term>Techniques de diagnostic moléculaire ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA Primers</term><term>DNA, Helminth</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Filarioidea</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN des helminthes</term><term>Amorces ADN</term><term>Filarioidea</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Filarioidea</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Filarioidea</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cattle</term><term>Computational Biology</term><term>Conserved Sequence</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Biologie informatique</term><term>Bovins</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term><term>Séquence conservée</term><term>Techniques de diagnostic moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lymphatic filariasis (LF) is a chronic disease and is caused by the parasites Wuchereria bancrofti (W. bancrofti), Brugia malayi (B. malayi) and Brugia timori (B. timori). In the present study, Setaria cervi (S. cervi), a bovine filarial parasite has been used. Previously, it has been reported that the S. cervi shares some common proteins and antigenic determinants with that of human filarial parasite. The larval stages of filarial species usually cannot be identified by classical morphology. Hence, molecular characterization allows the identification of the parasites throughout all their developmental stages. The genomic DNA of S. cervi adult were isolated and estimated spectrophotometrically for the quantitative presence of DNA content. Screening of DNA sequences from filarial DNA GenBank and Expressed Sequence Tags (EST's) were performed for homologous sequences and then multiple sequence alignment was executed. The conserved sequences from multiple sequence alignment were used for In Silico primer designing. The successfully designed primers were used further in PCR amplifications. Therefore, in search of a promising diagnostic tool few genes were identified to be conserved in the human and bovine filariasis and these novel primers deigned may help to develop a promising diagnostic tool for identification of lymphatic filariasis.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F79 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000F79 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Merge
|type= RBID
|clé= pubmed:26751881
|texte= In search of a potential diagnostic tool for molecular characterization of lymphatic filariasis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:26751881" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |