Evaluating the detection of circulating filarial antigen in diagnosis of bancroftian filariasis and filarial hydrocele.
Identifieur interne : 00BD28 ( Main/Exploration ); précédent : 00BD27; suivant : 00BD29Evaluating the detection of circulating filarial antigen in diagnosis of bancroftian filariasis and filarial hydrocele.
Auteurs : M M Hassan [Égypte] ; M. Ata ; R M Ramzy ; A E El-Gendi ; M H Hegab ; N S Gabr ; A F El-Chourbagy ; A A Mostafa ; A M NaguibSource :
- Journal of the Egyptian Society of Parasitology [ 1110-0583 ] ; 1996.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Filariose lymphatique, Hydrocèle.
- immunologie : Anticorps antihelminthe, Anticorps monoclonaux, Wuchereria bancrofti.
- sang : Antigènes d'helminthe.
- Animaux, Humains, Mâle, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antigens, Helminth.
- chemical , immunology : Antibodies, Helminth, Antibodies, Monoclonal.
- diagnosis : Elephantiasis, Filarial, Testicular Hydrocele.
- immunology : Wuchereria bancrofti.
- Animals, Humans, Male, Sensitivity and Specificity.
Abstract
Four groups of patients were selected: 16 patients with clinical evidence of obstructive filarial lymphangiopathy without microfilaraemia; 12 patients with clinical evidence of obstructive filarial lymphangiopathy with microfilaraemia and 9 patients with microfilaraemia. Two control groups were also included. Blood films, sera and hydrocele fluid samples were collected from all subjects. Polyclonal antibody against Dirofilaria immitis worm homogenate was prepared, fractionated and conjugated with HRP. Both polyclonal antibody and monoclonal antibody (AD12) were used in a sandwich ELISA. Using polyclonal antibody, both microfilaraemic groups (groups 2 and 3) had a significantly higher mean O.D. readings than that of control groups (P < 0.05), whereas, the mean O.D. readings of patients with symptomatic amicrofilaraemia had no significant difference than control groups. Symptomatic microfilaraemic group had the highest percentage of antigen positivity 7/12 (58.3%) among all groups while symptomatic amicrofilaraemic group had the least antigen positivity 2/16 (12.5%). Patients presented with elephantiasis only or with hydrocele had no antigen positive levels 0/12 (0%) in their serum or hydrocele fluid samples. On the other hand, 2 out of 4 cases represented with hydrocele (50%) had positive antigen levels in their hydrocele fluid samples. Using monoclonal antibody, all groups had a highly significant higher mean optical density readings than control groups. Asymptomatic microfilaraemic group had the highest percentage of antigen positivity 8/9 (88.9%), followed by symptomatic microfilaraemic 8/12 (66.7%), then symptomatic amicrofilaraemic group 4/16 (25%). The same 2 patients (amicrofilaraemia) and 7 (microfilaraemia) represented with hydrocele had positive antigen levels in their hydrocele fluid samples.
PubMed: 8918042
Affiliations:
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Le document en format XML
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Ata</name></author><author><name sortKey="Ramzy, R M" sort="Ramzy, R M" uniqKey="Ramzy R" first="R M" last="Ramzy">R M Ramzy</name></author><author><name sortKey="El Gendi, A E" sort="El Gendi, A E" uniqKey="El Gendi A" first="A E" last="El-Gendi">A E El-Gendi</name></author><author><name sortKey="Hegab, M H" sort="Hegab, M H" uniqKey="Hegab M" first="M H" last="Hegab">M H Hegab</name></author><author><name sortKey="Gabr, N S" sort="Gabr, N S" uniqKey="Gabr N" first="N S" last="Gabr">N S Gabr</name></author><author><name sortKey="El Chourbagy, A F" sort="El Chourbagy, A F" uniqKey="El Chourbagy A" first="A F" last="El-Chourbagy">A F El-Chourbagy</name></author><author><name sortKey="Mostafa, A A" sort="Mostafa, A A" uniqKey="Mostafa A" first="A A" last="Mostafa">A A Mostafa</name></author><author><name sortKey="Naguib, A M" sort="Naguib, A M" uniqKey="Naguib A" first="A M" last="Naguib">A M Naguib</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:8918042</idno><idno type="pmid">8918042</idno><idno type="wicri:Area/PubMed/Corpus">005092</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">005092</idno><idno type="wicri:Area/PubMed/Curation">005092</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">005092</idno><idno type="wicri:Area/PubMed/Checkpoint">005092</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">005092</idno><idno type="wicri:Area/Ncbi/Merge">00B870</idno><idno type="wicri:Area/Ncbi/Curation">00B870</idno><idno type="wicri:Area/Ncbi/Checkpoint">00B870</idno><idno type="wicri:doubleKey">1110-0583:1996:Hassan M:evaluating:the:detection</idno><idno type="wicri:Area/Main/Merge">00C563</idno><idno type="wicri:Area/Main/Curation">00BD28</idno><idno type="wicri:Area/Main/Exploration">00BD28</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Evaluating the detection of circulating filarial antigen in diagnosis of bancroftian filariasis and filarial hydrocele.</title><author><name sortKey="Hassan, M M" sort="Hassan, M M" uniqKey="Hassan M" first="M M" last="Hassan">M M Hassan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Parasitology, Faculty of Medicine, University, Egypt.</nlm:affiliation><country xml:lang="fr">Égypte</country><wicri:regionArea>Department of Parasitology, Faculty of Medicine, University</wicri:regionArea><wicri:noRegion>University</wicri:noRegion></affiliation></author><author><name sortKey="Ata, M" sort="Ata, M" uniqKey="Ata M" first="M" last="Ata">M. Ata</name></author><author><name sortKey="Ramzy, R M" sort="Ramzy, R M" uniqKey="Ramzy R" first="R M" last="Ramzy">R M Ramzy</name></author><author><name sortKey="El Gendi, A E" sort="El Gendi, A E" uniqKey="El Gendi A" first="A E" last="El-Gendi">A E El-Gendi</name></author><author><name sortKey="Hegab, M H" sort="Hegab, M H" uniqKey="Hegab M" first="M H" last="Hegab">M H Hegab</name></author><author><name sortKey="Gabr, N S" sort="Gabr, N S" uniqKey="Gabr N" first="N S" last="Gabr">N S Gabr</name></author><author><name sortKey="El Chourbagy, A F" sort="El Chourbagy, A F" uniqKey="El Chourbagy A" first="A F" last="El-Chourbagy">A F El-Chourbagy</name></author><author><name sortKey="Mostafa, A A" sort="Mostafa, A A" uniqKey="Mostafa A" first="A A" last="Mostafa">A A Mostafa</name></author><author><name sortKey="Naguib, A M" sort="Naguib, A M" uniqKey="Naguib A" first="A M" last="Naguib">A M Naguib</name></author></analytic><series><title level="j">Journal of the Egyptian Society of Parasitology</title><idno type="ISSN">1110-0583</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (immunology)</term><term>Antibodies, Monoclonal (immunology)</term><term>Antigens, Helminth (blood)</term><term>Elephantiasis, Filarial (diagnosis)</term><term>Humans</term><term>Male</term><term>Sensitivity and Specificity</term><term>Testicular Hydrocele (diagnosis)</term><term>Wuchereria bancrofti (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (immunologie)</term><term>Anticorps monoclonaux (immunologie)</term><term>Antigènes d'helminthe (sang)</term><term>Filariose lymphatique (diagnostic)</term><term>Humains</term><term>Hydrocèle (diagnostic)</term><term>Mâle</term><term>Sensibilité et spécificité</term><term>Wuchereria bancrofti (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antigens, Helminth</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Helminth</term><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Elephantiasis, Filarial</term><term>Testicular Hydrocele</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Filariose lymphatique</term><term>Hydrocèle</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Anticorps monoclonaux</term><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Wuchereria bancrofti</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Antigènes d'helminthe</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Humans</term><term>Male</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Mâle</term><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Four groups of patients were selected: 16 patients with clinical evidence of obstructive filarial lymphangiopathy without microfilaraemia; 12 patients with clinical evidence of obstructive filarial lymphangiopathy with microfilaraemia and 9 patients with microfilaraemia. Two control groups were also included. Blood films, sera and hydrocele fluid samples were collected from all subjects. Polyclonal antibody against Dirofilaria immitis worm homogenate was prepared, fractionated and conjugated with HRP. Both polyclonal antibody and monoclonal antibody (AD12) were used in a sandwich ELISA. Using polyclonal antibody, both microfilaraemic groups (groups 2 and 3) had a significantly higher mean O.D. readings than that of control groups (P < 0.05), whereas, the mean O.D. readings of patients with symptomatic amicrofilaraemia had no significant difference than control groups. Symptomatic microfilaraemic group had the highest percentage of antigen positivity 7/12 (58.3%) among all groups while symptomatic amicrofilaraemic group had the least antigen positivity 2/16 (12.5%). Patients presented with elephantiasis only or with hydrocele had no antigen positive levels 0/12 (0%) in their serum or hydrocele fluid samples. On the other hand, 2 out of 4 cases represented with hydrocele (50%) had positive antigen levels in their hydrocele fluid samples. Using monoclonal antibody, all groups had a highly significant higher mean optical density readings than control groups. Asymptomatic microfilaraemic group had the highest percentage of antigen positivity 8/9 (88.9%), followed by symptomatic microfilaraemic 8/12 (66.7%), then symptomatic amicrofilaraemic group 4/16 (25%). The same 2 patients (amicrofilaraemia) and 7 (microfilaraemia) represented with hydrocele had positive antigen levels in their hydrocele fluid samples.</div></front></TEI><affiliations><list><country><li>Égypte</li></country></list><tree><noCountry><name sortKey="Ata, M" sort="Ata, M" uniqKey="Ata M" first="M" last="Ata">M. Ata</name><name sortKey="El Chourbagy, A F" sort="El Chourbagy, A F" uniqKey="El Chourbagy A" first="A F" last="El-Chourbagy">A F El-Chourbagy</name><name sortKey="El Gendi, A E" sort="El Gendi, A E" uniqKey="El Gendi A" first="A E" last="El-Gendi">A E El-Gendi</name><name sortKey="Gabr, N S" sort="Gabr, N S" uniqKey="Gabr N" first="N S" last="Gabr">N S Gabr</name><name sortKey="Hegab, M H" sort="Hegab, M H" uniqKey="Hegab M" first="M H" last="Hegab">M H Hegab</name><name sortKey="Mostafa, A A" sort="Mostafa, A A" uniqKey="Mostafa A" first="A A" last="Mostafa">A A Mostafa</name><name sortKey="Naguib, A M" sort="Naguib, A M" uniqKey="Naguib A" first="A M" last="Naguib">A M Naguib</name><name sortKey="Ramzy, R M" sort="Ramzy, R M" uniqKey="Ramzy R" first="R M" last="Ramzy">R M Ramzy</name></noCountry><country name="Égypte"><noRegion><name sortKey="Hassan, M M" sort="Hassan, M M" uniqKey="Hassan M" first="M M" last="Hassan">M M Hassan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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