EphB4 forward signalling regulates lymphatic valve development
Identifieur interne : 001E30 ( Main/Exploration ); précédent : 001E29; suivant : 001E31EphB4 forward signalling regulates lymphatic valve development
Auteurs : Gu Zhang [États-Unis] ; John Brady [États-Unis] ; Wei-Ching Liang [États-Unis] ; Yan Wu [États-Unis] ; Mark Henkemeyer [États-Unis] ; Minhong Yan [États-Unis]Source :
- Nature Communications [ 2041-1723 ] ; 2015.
Abstract
Bidirectional signalling is regarded as a notable hallmark of the Eph-ephrin signalling system: Eph-dependent forward signalling in Eph-expressing cells and ephrin-dependent reverse signalling in Ephrin-expressing cells. The notion of ephrin-dependent reverse signalling derives from genetic experiments utilizing mice carrying mutations in the intracellular region of ephrinBs. Here we show that EphB4-dependent forward signalling regulates lymphatic valve development, a process previously thought to be regulated by ephrinB2-dependent reverse signalling. We develop antibodies that selectively target EphB4 and ephrinB2. We find that mice bearing genetically altered cytoplasmic region of ephrinB2 have significantly altered EphB4-dependent forward signalling. Selective inhibition of EphB4 using a functional blocking antibody results in defective lymphatic valve development. Furthermore, a chemical genetic approach is used to unequivocally show that the kinase activity of EphB4 is essential for lymphatic valve development.
Url:
DOI: 10.1038/ncomms7625
PubMed: 25865237
PubMed Central: 4403310
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>Bidirectional signalling is regarded as a notable hallmark of the Eph-ephrin signalling system: Eph-dependent forward signalling in Eph-expressing cells and ephrin-dependent reverse signalling in Ephrin-expressing cells. The notion of ephrin-dependent reverse signalling derives from genetic experiments utilizing mice carrying mutations in the intracellular region of ephrinBs. Here we show that EphB4-dependent forward signalling regulates lymphatic valve development, a process previously thought to be regulated by ephrinB2-dependent reverse signalling. We develop antibodies that selectively target EphB4 and ephrinB2. We find that mice bearing genetically altered cytoplasmic region of ephrinB2 have significantly altered EphB4-dependent forward signalling. Selective inhibition of EphB4 using a functional blocking antibody results in defective lymphatic valve development. Furthermore, a chemical genetic approach is used to unequivocally show that the kinase activity of EphB4 is essential for lymphatic valve development.</p>
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<author><name sortKey="Tammela, T" uniqKey="Tammela T">T. Tammela</name>
</author>
<author><name sortKey="Petrova, T V" uniqKey="Petrova T">T. V. Petrova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Choi, I" uniqKey="Choi I">I. Choi</name>
</author>
<author><name sortKey="Lee, S" uniqKey="Lee S">S. Lee</name>
</author>
<author><name sortKey="Hong, Y K" uniqKey="Hong Y">Y. K. Hong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bazigou, E" uniqKey="Bazigou E">E. Bazigou</name>
</author>
<author><name sortKey="Makinen, T" uniqKey="Makinen T">T. Makinen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bazigou, E" uniqKey="Bazigou E">E. Bazigou</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bouvree, K" uniqKey="Bouvree K">K. Bouvree</name>
</author>
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</biblStruct>
<biblStruct><analytic><author><name sortKey="Jurisic, G" uniqKey="Jurisic G">G. Jurisic</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kanady, J D" uniqKey="Kanady J">J. D. Kanady</name>
</author>
<author><name sortKey="Dellinger, M T" uniqKey="Dellinger M">M. T. Dellinger</name>
</author>
<author><name sortKey="Munger, S J" uniqKey="Munger S">S. J. Munger</name>
</author>
<author><name sortKey="Witte, M H" uniqKey="Witte M">M. H. Witte</name>
</author>
<author><name sortKey="Simon, A M" uniqKey="Simon A">A. M. Simon</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Levet, S" uniqKey="Levet S">S. Levet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Makinen, T" uniqKey="Makinen T">T. Makinen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Petrova, T V" uniqKey="Petrova T">T. V. Petrova</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sabine, A" uniqKey="Sabine A">A. Sabine</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pasquale, E B" uniqKey="Pasquale E">E. B. Pasquale</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pitulescu, M E" uniqKey="Pitulescu M">M. E. Pitulescu</name>
</author>
<author><name sortKey="Adams, R H" uniqKey="Adams R">R. H. Adams</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, H U" uniqKey="Wang H">H. U. Wang</name>
</author>
<author><name sortKey="Chen, Z F" uniqKey="Chen Z">Z. F. Chen</name>
</author>
<author><name sortKey="Anderson, D J" uniqKey="Anderson D">D. J. Anderson</name>
</author>
</analytic>
</biblStruct>
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</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Davy, A" uniqKey="Davy A">A. Davy</name>
</author>
<author><name sortKey="Soriano, P" uniqKey="Soriano P">P. Soriano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Armstrong, J N" uniqKey="Armstrong J">J. N. Armstrong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bouzioukh, F" uniqKey="Bouzioukh F">F. Bouzioukh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bush, J O" uniqKey="Bush J">J. O. Bush</name>
</author>
<author><name sortKey="Soriano, P" uniqKey="Soriano P">P. Soriano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dravis, C" uniqKey="Dravis C">C. Dravis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xu, N J" uniqKey="Xu N">N. J. Xu</name>
</author>
<author><name sortKey="Henkemeyer, M" uniqKey="Henkemeyer M">M. Henkemeyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Xu, N J" uniqKey="Xu N">N. J. Xu</name>
</author>
<author><name sortKey="Sun, S" uniqKey="Sun S">S. Sun</name>
</author>
<author><name sortKey="Gibson, J R" uniqKey="Gibson J">J. R. Gibson</name>
</author>
<author><name sortKey="Henkemeyer, M" uniqKey="Henkemeyer M">M. Henkemeyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bazigou, E" uniqKey="Bazigou E">E. Bazigou</name>
</author>
</analytic>
</biblStruct>
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</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gerety, S S" uniqKey="Gerety S">S. S. Gerety</name>
</author>
<author><name sortKey="Wang, H U" uniqKey="Wang H">H. U. Wang</name>
</author>
<author><name sortKey="Chen, Z F" uniqKey="Chen Z">Z. F. Chen</name>
</author>
<author><name sortKey="Anderson, D J" uniqKey="Anderson D">D. J. Anderson</name>
</author>
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</author>
<author><name sortKey="Henkemeyer, M" uniqKey="Henkemeyer M">M. Henkemeyer</name>
</author>
</analytic>
</biblStruct>
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</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cowan, C A" uniqKey="Cowan C">C. A. Cowan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dravis, C" uniqKey="Dravis C">C. Dravis</name>
</author>
<author><name sortKey="Henkemeyer, M" uniqKey="Henkemeyer M">M. Henkemeyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Martiny Baron, G" uniqKey="Martiny Baron G">G. Martiny-Baron</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gallion, S L" uniqKey="Gallion S">S. L. Gallion</name>
</author>
<author><name sortKey="Qian, D" uniqKey="Qian D">D. Qian</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Williams, R" uniqKey="Williams R">R. Williams</name>
</author>
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</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Katsuta, H" uniqKey="Katsuta H">H. Katsuta</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, C V" uniqKey="Lee C">C. V. Lee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liang, W C" uniqKey="Liang W">W. C. Liang</name>
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