IgE-binding components in Pityrosporum orbiculare identified by an immunoblotting technique.
Identifieur interne : 00D537 ( Main/Exploration ); précédent : 00D536; suivant : 00D538IgE-binding components in Pityrosporum orbiculare identified by an immunoblotting technique.
Auteurs : S. Johansson [Suède] ; K. KarlströmSource :
- Acta dermato-venereologica [ 0001-5555 ] ; 1991.
Descripteurs français
- KwdFr :
- Adulte, Allergènes (analyse), Cellules cultivées, Eczéma atopique (immunologie), Filariose lymphatique (immunologie), Humains, Immunoglobuline E (immunologie), Immunotransfert, Malassezia (immunologie), Pityriasis versicolor (immunologie), Sites de fixation des anticorps, Test RAST, Électrophorèse sur gel de polyacrylamide.
- MESH :
English descriptors
- KwdEn :
- Adult, Allergens (analysis), Binding Sites, Antibody, Cells, Cultured, Dermatitis, Atopic (immunology), Electrophoresis, Polyacrylamide Gel, Elephantiasis, Filarial (immunology), Humans, Immunoblotting, Immunoglobulin E (immunology), Malassezia (immunology), Radioallergosorbent Test, Tinea Versicolor (immunology).
- MESH :
- chemical , analysis : Allergens.
- immunology : Dermatitis, Atopic, Elephantiasis, Filarial, Immunoglobulin E, Malassezia, Tinea Versicolor.
- Adult, Binding Sites, Antibody, Cells, Cultured, Electrophoresis, Polyacrylamide Gel, Humans, Immunoblotting, Radioallergosorbent Test.
Abstract
IgE-binding components in crude extracts of Pityrosporum orbiculare were separated by sodium dodecylsulphate polyacrylamide gel electrophoresis and transferred to Immobilon membrane. The components were detected by P. orbiculare radioallergosorbent (RAST) positive sera (class 2-4) from 37 patients with atopic dermatitis and visualized by enzyme-labelled anti-human IgE antibodies in chromogenic substrate. Numerous IgE-binding compounds were demonstrated in the molecular weight (MW) range of 14-94 kD. Eight of them were characterized to molecular size, frequency of reacting sera and intensity of bands. Six of the defined components were found to be major allergens reacting with more than 50% of the sera. They had MWs 86, 76, 67, 28, 17 and 13 kD. Thirty-six of the 37 patients had elevated total serum IgE. Two other patient categories were studied; 11 patients with lymphatic filariasis (high total serum IgE, greater than 1,300 kU/l) and 15 pityriasis versicolor patients (normal total serum IgE, less than 122 kU/l). The P. orbiculare RAST values for these sera showed good accordance with the reactivity in immunoblotting experiments.
PubMed: 1676206
Affiliations:
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Johansson</name><affiliation wicri:level="3"><nlm:affiliation>Department of Clinical Immunology, Karolinska Institute, Karolinska Hospital, Stockholm, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Clinical Immunology, Karolinska Institute, Karolinska Hospital, Stockholm</wicri:regionArea><placeName><settlement type="city">Stockholm</settlement><region nuts="2">Svealand</region></placeName></affiliation></author><author><name sortKey="Karlstrom, K" sort="Karlstrom, K" uniqKey="Karlstrom K" first="K" last="Karlström">K. Karlström</name></author></analytic><series><title level="j">Acta dermato-venereologica</title><idno type="ISSN">0001-5555</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Allergens (analysis)</term><term>Binding Sites, Antibody</term><term>Cells, Cultured</term><term>Dermatitis, Atopic (immunology)</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Elephantiasis, Filarial (immunology)</term><term>Humans</term><term>Immunoblotting</term><term>Immunoglobulin E (immunology)</term><term>Malassezia (immunology)</term><term>Radioallergosorbent Test</term><term>Tinea Versicolor (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Allergènes (analyse)</term><term>Cellules cultivées</term><term>Eczéma atopique (immunologie)</term><term>Filariose lymphatique (immunologie)</term><term>Humains</term><term>Immunoglobuline E (immunologie)</term><term>Immunotransfert</term><term>Malassezia (immunologie)</term><term>Pityriasis versicolor (immunologie)</term><term>Sites de fixation des anticorps</term><term>Test RAST</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Allergens</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Allergènes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Eczéma atopique</term><term>Filariose lymphatique</term><term>Immunoglobuline E</term><term>Malassezia</term><term>Pityriasis versicolor</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Dermatitis, Atopic</term><term>Elephantiasis, Filarial</term><term>Immunoglobulin E</term><term>Malassezia</term><term>Tinea Versicolor</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Binding Sites, Antibody</term><term>Cells, Cultured</term><term>Electrophoresis, Polyacrylamide Gel</term><term>Humans</term><term>Immunoblotting</term><term>Radioallergosorbent Test</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Cellules cultivées</term><term>Humains</term><term>Immunotransfert</term><term>Sites de fixation des anticorps</term><term>Test RAST</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">IgE-binding components in crude extracts of Pityrosporum orbiculare were separated by sodium dodecylsulphate polyacrylamide gel electrophoresis and transferred to Immobilon membrane. The components were detected by P. orbiculare radioallergosorbent (RAST) positive sera (class 2-4) from 37 patients with atopic dermatitis and visualized by enzyme-labelled anti-human IgE antibodies in chromogenic substrate. Numerous IgE-binding compounds were demonstrated in the molecular weight (MW) range of 14-94 kD. Eight of them were characterized to molecular size, frequency of reacting sera and intensity of bands. Six of the defined components were found to be major allergens reacting with more than 50% of the sera. They had MWs 86, 76, 67, 28, 17 and 13 kD. Thirty-six of the 37 patients had elevated total serum IgE. Two other patient categories were studied; 11 patients with lymphatic filariasis (high total serum IgE, greater than 1,300 kU/l) and 15 pityriasis versicolor patients (normal total serum IgE, less than 122 kU/l). The P. orbiculare RAST values for these sera showed good accordance with the reactivity in immunoblotting experiments.</div></front></TEI><affiliations><list><country><li>Suède</li></country><region><li>Svealand</li></region><settlement><li>Stockholm</li></settlement></list><tree><noCountry><name sortKey="Karlstrom, K" sort="Karlstrom, K" uniqKey="Karlstrom K" first="K" last="Karlström">K. Karlström</name></noCountry><country name="Suède"><region name="Svealand"><name sortKey="Johansson, S" sort="Johansson, S" uniqKey="Johansson S" first="S" last="Johansson">S. Johansson</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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