Detection of nodal micrometastases using immunohistochemistry and PCR in melanoma of the arm and trunk.
Identifieur interne : 009477 ( Main/Exploration ); précédent : 009476; suivant : 009478Detection of nodal micrometastases using immunohistochemistry and PCR in melanoma of the arm and trunk.
Auteurs : S. Boi [Italie] ; P. Cristofolini ; R. Togni ; S. Girlando ; M. Camerani ; D. Donner ; M. Cristofolini ; P. Dalla PalmaSource :
- Melanoma research [ 0960-8931 ] ; 2002.
Descripteurs français
- KwdFr :
- ARN messager (analyse), ARN tumoral (analyse), Biopsie de noeud lymphatique sentinelle, Femelle, Humains, Monophenol monooxygenase (génétique), Monophenol monooxygenase (métabolisme), Mâle, Mélanome (génétique), Mélanome (métabolisme), Mélanome (secondaire), Métastase lymphatique, Noeuds lymphatiques (anatomopathologie), Noeuds lymphatiques (métabolisme), RT-PCR, Scintigraphie, Stade de la tumeur, Techniques immunoenzymatiques, Tumeurs cutanées (anatomopathologie), Tumeurs cutanées (génétique), Tumeurs cutanées (métabolisme).
- MESH :
- analyse : ARN messager, ARN tumoral.
- anatomopathologie : Noeuds lymphatiques, Tumeurs cutanées.
- génétique : Monophenol monooxygenase, Mélanome, Tumeurs cutanées.
- métabolisme : Monophenol monooxygenase, Mélanome, Noeuds lymphatiques, Tumeurs cutanées.
- secondaire : Mélanome.
- Biopsie de noeud lymphatique sentinelle, Femelle, Humains, Mâle, Métastase lymphatique, RT-PCR, Scintigraphie, Stade de la tumeur, Techniques immunoenzymatiques.
English descriptors
- KwdEn :
- Female, Humans, Immunoenzyme Techniques, Lymph Nodes (metabolism), Lymph Nodes (pathology), Lymphatic Metastasis, Male, Melanoma (genetics), Melanoma (metabolism), Melanoma (secondary), Monophenol Monooxygenase (genetics), Monophenol Monooxygenase (metabolism), Neoplasm Staging, RNA, Messenger (analysis), RNA, Neoplasm (analysis), Radionuclide Imaging, Reverse Transcriptase Polymerase Chain Reaction, Sentinel Lymph Node Biopsy, Skin Neoplasms (genetics), Skin Neoplasms (metabolism), Skin Neoplasms (pathology).
- MESH :
- chemical , analysis : RNA, Messenger, RNA, Neoplasm.
- chemical , genetics : Monophenol Monooxygenase.
- genetics : Melanoma, Skin Neoplasms.
- metabolism : Lymph Nodes, Melanoma, Monophenol Monooxygenase, Skin Neoplasms.
- pathology : Lymph Nodes, Skin Neoplasms.
- secondary : Melanoma.
- Female, Humans, Immunoenzyme Techniques, Lymphatic Metastasis, Male, Neoplasm Staging, Radionuclide Imaging, Reverse Transcriptase Polymerase Chain Reaction, Sentinel Lymph Node Biopsy.
Abstract
Sentinel node (SN) mapping and biopsy seems at present the best way to assess the nodal status in cutaneous melanoma without removing the lymphatic chain. The procedure is minimally invasive, safe and low cost, and allows selection of patients who can benefit from elective node dissection. From March 1997 up to July 1999 we examined 112 SNs excised after lymphatic mapping from 95 patients (48 males and 47 females) with stage I cutaneous melanoma affecting the trunk or limbs. Of these, 88 SNs from 74 patients were submitted to polymerase chain reaction (PCR) in order to detect tyrosinase mRNA. A new antibody (anti-tyrosinase, Clone T311, IgG2a type, Lab Vision Corporation) was used to detect nodal micrometastases. The search for micrometastases was histologically positive in 15 SNs and negative in 97. The 88 SNs examined using molecular biology were positive in 40 cases and negative in 48. In 28 only the PCR was positive. The new antibody used to detect micrometastases was shown to be very useful. Cases positive on both conventional histology and PCR were Clark level II or more and were thicker than 0.6 mm. No difference with regard to site or sex was observed. Lymphoedema and hypersensitivity reactions, nor the inability to work, did not occur. Only patients with histologically proven micrometastases underwent elective node dissection. Cases positive only on molecular biology were submitted to close follow-up.
PubMed: 11930111
Affiliations:
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Le document en format XML
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Togni</name></author><author><name sortKey="Girlando, S" sort="Girlando, S" uniqKey="Girlando S" first="S" last="Girlando">S. Girlando</name></author><author><name sortKey="Camerani, M" sort="Camerani, M" uniqKey="Camerani M" first="M" last="Camerani">M. Camerani</name></author><author><name sortKey="Donner, D" sort="Donner, D" uniqKey="Donner D" first="D" last="Donner">D. Donner</name></author><author><name sortKey="Cristofolini, M" sort="Cristofolini, M" uniqKey="Cristofolini M" first="M" last="Cristofolini">M. Cristofolini</name></author><author><name sortKey="Dalla Palma, P" sort="Dalla Palma, P" uniqKey="Dalla Palma P" first="P" last="Dalla Palma">P. Dalla Palma</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:11930111</idno><idno type="pmid">11930111</idno><idno type="wicri:Area/PubMed/Corpus">004626</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">004626</idno><idno type="wicri:Area/PubMed/Curation">004626</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">004626</idno><idno type="wicri:Area/PubMed/Checkpoint">004626</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">004626</idno><idno type="wicri:Area/Ncbi/Merge">000914</idno><idno type="wicri:Area/Ncbi/Curation">000914</idno><idno type="wicri:Area/Ncbi/Checkpoint">000914</idno><idno type="wicri:doubleKey">0960-8931:2002:Boi S:detection:of:nodal</idno><idno type="wicri:Area/Main/Merge">009843</idno><idno type="wicri:Area/Main/Curation">009477</idno><idno type="wicri:Area/Main/Exploration">009477</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of nodal micrometastases using immunohistochemistry and PCR in melanoma of the arm and trunk.</title><author><name sortKey="Boi, S" sort="Boi, S" uniqKey="Boi S" first="S" last="Boi">S. Boi</name><affiliation wicri:level="1"><nlm:affiliation>Departments of Pathology, Santa Chiara Hospital, 38100 Trento, Italy. boi@tn.aziendasanitaria.trentino.it.</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Departments of Pathology, Santa Chiara Hospital, 38100 Trento</wicri:regionArea><wicri:noRegion>38100 Trento</wicri:noRegion></affiliation></author><author><name sortKey="Cristofolini, P" sort="Cristofolini, P" uniqKey="Cristofolini P" first="P" last="Cristofolini">P. Cristofolini</name></author><author><name sortKey="Togni, R" sort="Togni, R" uniqKey="Togni R" first="R" last="Togni">R. Togni</name></author><author><name sortKey="Girlando, S" sort="Girlando, S" uniqKey="Girlando S" first="S" last="Girlando">S. Girlando</name></author><author><name sortKey="Camerani, M" sort="Camerani, M" uniqKey="Camerani M" first="M" last="Camerani">M. Camerani</name></author><author><name sortKey="Donner, D" sort="Donner, D" uniqKey="Donner D" first="D" last="Donner">D. Donner</name></author><author><name sortKey="Cristofolini, M" sort="Cristofolini, M" uniqKey="Cristofolini M" first="M" last="Cristofolini">M. Cristofolini</name></author><author><name sortKey="Dalla Palma, P" sort="Dalla Palma, P" uniqKey="Dalla Palma P" first="P" last="Dalla Palma">P. Dalla Palma</name></author></analytic><series><title level="j">Melanoma research</title><idno type="ISSN">0960-8931</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Female</term><term>Humans</term><term>Immunoenzyme Techniques</term><term>Lymph Nodes (metabolism)</term><term>Lymph Nodes (pathology)</term><term>Lymphatic Metastasis</term><term>Male</term><term>Melanoma (genetics)</term><term>Melanoma (metabolism)</term><term>Melanoma (secondary)</term><term>Monophenol Monooxygenase (genetics)</term><term>Monophenol Monooxygenase (metabolism)</term><term>Neoplasm Staging</term><term>RNA, Messenger (analysis)</term><term>RNA, Neoplasm (analysis)</term><term>Radionuclide Imaging</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sentinel Lymph Node Biopsy</term><term>Skin Neoplasms (genetics)</term><term>Skin Neoplasms (metabolism)</term><term>Skin Neoplasms (pathology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (analyse)</term><term>ARN tumoral (analyse)</term><term>Biopsie de noeud lymphatique sentinelle</term><term>Femelle</term><term>Humains</term><term>Monophenol monooxygenase (génétique)</term><term>Monophenol monooxygenase (métabolisme)</term><term>Mâle</term><term>Mélanome (génétique)</term><term>Mélanome (métabolisme)</term><term>Mélanome (secondaire)</term><term>Métastase lymphatique</term><term>Noeuds lymphatiques (anatomopathologie)</term><term>Noeuds lymphatiques (métabolisme)</term><term>RT-PCR</term><term>Scintigraphie</term><term>Stade de la tumeur</term><term>Techniques immunoenzymatiques</term><term>Tumeurs cutanées (anatomopathologie)</term><term>Tumeurs cutanées (génétique)</term><term>Tumeurs cutanées (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Messenger</term><term>RNA, Neoplasm</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Monophenol Monooxygenase</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN messager</term><term>ARN tumoral</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Noeuds lymphatiques</term><term>Tumeurs cutanées</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Melanoma</term><term>Skin Neoplasms</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Monophenol monooxygenase</term><term>Mélanome</term><term>Tumeurs cutanées</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lymph Nodes</term><term>Melanoma</term><term>Monophenol Monooxygenase</term><term>Skin Neoplasms</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Monophenol monooxygenase</term><term>Mélanome</term><term>Noeuds lymphatiques</term><term>Tumeurs cutanées</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Lymph Nodes</term><term>Skin Neoplasms</term></keywords><keywords scheme="MESH" qualifier="secondaire" xml:lang="fr"><term>Mélanome</term></keywords><keywords scheme="MESH" qualifier="secondary" xml:lang="en"><term>Melanoma</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Female</term><term>Humans</term><term>Immunoenzyme Techniques</term><term>Lymphatic Metastasis</term><term>Male</term><term>Neoplasm Staging</term><term>Radionuclide Imaging</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sentinel Lymph Node Biopsy</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biopsie de noeud lymphatique sentinelle</term><term>Femelle</term><term>Humains</term><term>Mâle</term><term>Métastase lymphatique</term><term>RT-PCR</term><term>Scintigraphie</term><term>Stade de la tumeur</term><term>Techniques immunoenzymatiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Sentinel node (SN) mapping and biopsy seems at present the best way to assess the nodal status in cutaneous melanoma without removing the lymphatic chain. The procedure is minimally invasive, safe and low cost, and allows selection of patients who can benefit from elective node dissection. From March 1997 up to July 1999 we examined 112 SNs excised after lymphatic mapping from 95 patients (48 males and 47 females) with stage I cutaneous melanoma affecting the trunk or limbs. Of these, 88 SNs from 74 patients were submitted to polymerase chain reaction (PCR) in order to detect tyrosinase mRNA. A new antibody (anti-tyrosinase, Clone T311, IgG2a type, Lab Vision Corporation) was used to detect nodal micrometastases. The search for micrometastases was histologically positive in 15 SNs and negative in 97. The 88 SNs examined using molecular biology were positive in 40 cases and negative in 48. In 28 only the PCR was positive. The new antibody used to detect micrometastases was shown to be very useful. Cases positive on both conventional histology and PCR were Clark level II or more and were thicker than 0.6 mm. No difference with regard to site or sex was observed. Lymphoedema and hypersensitivity reactions, nor the inability to work, did not occur. Only patients with histologically proven micrometastases underwent elective node dissection. Cases positive only on molecular biology were submitted to close follow-up.</div></front></TEI><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Camerani, M" sort="Camerani, M" uniqKey="Camerani M" first="M" last="Camerani">M. Camerani</name><name sortKey="Cristofolini, M" sort="Cristofolini, M" uniqKey="Cristofolini M" first="M" last="Cristofolini">M. Cristofolini</name><name sortKey="Cristofolini, P" sort="Cristofolini, P" uniqKey="Cristofolini P" first="P" last="Cristofolini">P. Cristofolini</name><name sortKey="Dalla Palma, P" sort="Dalla Palma, P" uniqKey="Dalla Palma P" first="P" last="Dalla Palma">P. Dalla Palma</name><name sortKey="Donner, D" sort="Donner, D" uniqKey="Donner D" first="D" last="Donner">D. Donner</name><name sortKey="Girlando, S" sort="Girlando, S" uniqKey="Girlando S" first="S" last="Girlando">S. Girlando</name><name sortKey="Togni, R" sort="Togni, R" uniqKey="Togni R" first="R" last="Togni">R. Togni</name></noCountry><country name="Italie"><noRegion><name sortKey="Boi, S" sort="Boi, S" uniqKey="Boi S" first="S" last="Boi">S. Boi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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