Immunoprophylactic evaluation of a 37-kDa Brugia malayi recombinant antigen in lymphatic filariasis.
Identifieur interne : 007957 ( Main/Exploration ); précédent : 007956; suivant : 007958Immunoprophylactic evaluation of a 37-kDa Brugia malayi recombinant antigen in lymphatic filariasis.
Auteurs : P. Dabir [Inde] ; S. Dabir ; K N Krithika ; K. Goswami ; M V R. ReddySource :
- Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [ 1198-743X ] ; 2006.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), Activation des lymphocytes, Animaux, Anticorps antihelminthe (sang), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Brugia malayi (génétique), Brugia malayi (immunologie), Clonage moléculaire, Cytotoxicité immunologique, Filariose lymphatique (), Filariose lymphatique (immunologie), Gerbillinae, Gènes d'helminthe, Humains, Immunisation, Immunoglobuline G (sang), Interféron gamma (métabolisme), Lymphocytes auxiliaires Th1 (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Sérums immuns (immunologie).
- MESH :
- génétique : ADN complémentaire, Antigènes d'helminthe, Brugia malayi, Protéines recombinantes.
- immunologie : Antigènes d'helminthe, Brugia malayi, Filariose lymphatique, Lymphocytes auxiliaires Th1, Protéines recombinantes, Sérums immuns.
- métabolisme : Interféron gamma.
- sang : Anticorps antihelminthe, Immunoglobuline G.
- Activation des lymphocytes, Animaux, Clonage moléculaire, Cytotoxicité immunologique, Filariose lymphatique, Gerbillinae, Gènes d'helminthe, Humains, Immunisation.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Brugia malayi (genetics), Brugia malayi (immunology), Cloning, Molecular, Cytotoxicity, Immunologic, DNA, Complementary (genetics), Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (prevention & control), Genes, Helminth, Gerbillinae, Humans, Immune Sera (immunology), Immunization, Immunoglobulin G (blood), Interferon-gamma (metabolism), Lymphocyte Activation, Recombinant Proteins (genetics), Recombinant Proteins (immunology), Th1 Cells (immunology).
- MESH :
- chemical , blood : Antibodies, Helminth, Immunoglobulin G.
- chemical , genetics : Antigens, Helminth, DNA, Complementary, Recombinant Proteins.
- chemical , immunology : Antigens, Helminth, Immune Sera, Recombinant Proteins.
- genetics : Brugia malayi.
- immunology : Brugia malayi, Elephantiasis, Filarial, Th1 Cells.
- chemical , metabolism : Interferon-gamma.
- prevention & control : Elephantiasis, Filarial.
- Animals, Cloning, Molecular, Cytotoxicity, Immunologic, Genes, Helminth, Gerbillinae, Humans, Immunization, Lymphocyte Activation.
Abstract
The Brugia malayi filarial antigens recognised preferentially by sera from an endemic normal population are considered to be potential vaccine candidates. By immunoscreening the cDNA library of the infective L3 stage of B. malayi with pooled endemic normal sera, a cDNA clone Bm-SL3 was identified. Analysis of sera from different patient groups with the rBm-SL3 protein showed it to be highly reactive with endemic normal sera compared to its reactivity with microfilaraemic and non-endemic normal sera. The immunoprotective efficacy of the rBm-SL3 antigen against B. malayi filarial infection was evaluated in susceptible host jirds (gerbils) (Meriones unguiculatus). Jirds immunised with the rBm-SL3 antigen showed 68% cytotoxicity against microfilariae and 67-69% cytotoxicity against infective larvae in in-vitro antibody-dependent cellular cytotoxicity assays and in-situ micropore chamber methods. Analysis of IgG subclasses against Bm-SL3 revealed a significant increase in IgG1 and IgG2 antibodies in endemic normal sera compared with other groups. Lymphocyte proliferation to Bm-SL3 was significantly higher in the endemic normal group compared with that in clinical and microfilarial carriers (p < 0.001). Significantly enhanced levels of IFN-gamma in the culture supernatant of peripheral blood mononuclear cells of endemic normal sera after stimulation with Bm-SL3 suggest that the cellular response in this group may have a Th1 bias.
DOI: 10.1111/j.1469-0691.2006.01362.x
PubMed: 16524413
Affiliations:
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Reddy</name></author></analytic><series><title level="j">Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases</title><idno type="ISSN">1198-743X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Antigens, Helminth (genetics)</term><term>Antigens, Helminth (immunology)</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (immunology)</term><term>Cloning, Molecular</term><term>Cytotoxicity, Immunologic</term><term>DNA, Complementary (genetics)</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Genes, Helminth</term><term>Gerbillinae</term><term>Humans</term><term>Immune Sera (immunology)</term><term>Immunization</term><term>Immunoglobulin G (blood)</term><term>Interferon-gamma (metabolism)</term><term>Lymphocyte Activation</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>Th1 Cells (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>Activation des lymphocytes</term><term>Animaux</term><term>Anticorps antihelminthe (sang)</term><term>Antigènes d'helminthe (génétique)</term><term>Antigènes d'helminthe (immunologie)</term><term>Brugia malayi (génétique)</term><term>Brugia malayi (immunologie)</term><term>Clonage moléculaire</term><term>Cytotoxicité immunologique</term><term>Filariose lymphatique ()</term><term>Filariose lymphatique (immunologie)</term><term>Gerbillinae</term><term>Gènes d'helminthe</term><term>Humains</term><term>Immunisation</term><term>Immunoglobuline G (sang)</term><term>Interféron gamma (métabolisme)</term><term>Lymphocytes auxiliaires Th1 (immunologie)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Sérums immuns (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>DNA, Complementary</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term><term>Immune Sera</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Filariose lymphatique</term><term>Lymphocytes auxiliaires Th1</term><term>Protéines recombinantes</term><term>Sérums immuns</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Elephantiasis, Filarial</term><term>Th1 Cells</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Interferon-gamma</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Interféron gamma</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cloning, Molecular</term><term>Cytotoxicity, Immunologic</term><term>Genes, Helminth</term><term>Gerbillinae</term><term>Humans</term><term>Immunization</term><term>Lymphocyte Activation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation des lymphocytes</term><term>Animaux</term><term>Clonage moléculaire</term><term>Cytotoxicité immunologique</term><term>Filariose lymphatique</term><term>Gerbillinae</term><term>Gènes d'helminthe</term><term>Humains</term><term>Immunisation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Brugia malayi filarial antigens recognised preferentially by sera from an endemic normal population are considered to be potential vaccine candidates. By immunoscreening the cDNA library of the infective L3 stage of B. malayi with pooled endemic normal sera, a cDNA clone Bm-SL3 was identified. Analysis of sera from different patient groups with the rBm-SL3 protein showed it to be highly reactive with endemic normal sera compared to its reactivity with microfilaraemic and non-endemic normal sera. The immunoprotective efficacy of the rBm-SL3 antigen against B. malayi filarial infection was evaluated in susceptible host jirds (gerbils) (Meriones unguiculatus). Jirds immunised with the rBm-SL3 antigen showed 68% cytotoxicity against microfilariae and 67-69% cytotoxicity against infective larvae in in-vitro antibody-dependent cellular cytotoxicity assays and in-situ micropore chamber methods. Analysis of IgG subclasses against Bm-SL3 revealed a significant increase in IgG1 and IgG2 antibodies in endemic normal sera compared with other groups. Lymphocyte proliferation to Bm-SL3 was significantly higher in the endemic normal group compared with that in clinical and microfilarial carriers (p < 0.001). Significantly enhanced levels of IFN-gamma in the culture supernatant of peripheral blood mononuclear cells of endemic normal sera after stimulation with Bm-SL3 suggest that the cellular response in this group may have a Th1 bias.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Dabir, S" sort="Dabir, S" uniqKey="Dabir S" first="S" last="Dabir">S. Dabir</name><name sortKey="Goswami, K" sort="Goswami, K" uniqKey="Goswami K" first="K" last="Goswami">K. Goswami</name><name sortKey="Krithika, K N" sort="Krithika, K N" uniqKey="Krithika K" first="K N" last="Krithika">K N Krithika</name><name sortKey="Reddy, M V R" sort="Reddy, M V R" uniqKey="Reddy M" first="M V R" last="Reddy">M V R. Reddy</name></noCountry><country name="Inde"><noRegion><name sortKey="Dabir, P" sort="Dabir, P" uniqKey="Dabir P" first="P" last="Dabir">P. Dabir</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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