Use of a whole-slide imaging system to assess the presence and alteration of lymphatic vessels in joint sections of arthritic mice
Identifieur interne : 003D62 ( Main/Exploration ); précédent : 003D61; suivant : 003D63Use of a whole-slide imaging system to assess the presence and alteration of lymphatic vessels in joint sections of arthritic mice
Auteurs : J. Shi [République populaire de Chine, États-Unis] ; Q. Liang [États-Unis, République populaire de Chine] ; Y. Wang [République populaire de Chine] ; Ra Mooney [États-Unis] ; Bf Boyce [États-Unis] ; L. Xing [États-Unis]Source :
- Biotechnic & histochemistry : official publication of the Biological Stain Commission [ 1052-0295 ] ; 2012.
Abstract
We investigated the presence and alteration of lymphatic vessels in joints of arthritic mice using a whole-slide imaging system. Joints and long bone sections were cut from paraffin blocks of two mouse models of arthritis: meniscal-ligamentous injury (MLI)-induced osteoarthritis (OA) and TNF transgene (TNF-Tg)-induced rheumatoid arthritis (RA). MLI-OA mice were fed a high fat diet to accelerate OA development. TNF-Tg mice were treated with lymphatic growth factor VEGF-C virus to stimulate lymphangiogenesis. Sections were double immunofluorescence stained with anti-podoplanin and alpha-smooth muscle action. The area and number of lymphatic capillaries and mature lymphatic vessels were determined using a whole-slide imaging system and its associated software. Lymphatic vessels in joints were distributed in soft tissues mainly around the joint capsule, ligaments, fat pads and muscles. In long bones, enriched lymphatic vessels were present in the periosteal areas adjacent to the blood vessels. Occasionally, lymphatic vessels were observed in the cortical bone. Increased lymphatic capillaries, but decreased mature lymphatic vessels, were detected in both OA and RA joints. VEGF-C treatment increased lymphatic capillary and mature vessel formation in RA joints. We demonstrated decreased mature lymphatic vessels in the joints of mouse models of severe OA and RA. VEGF-C treatment increased the lymphatic vessel number and area in RA joints. Our findings suggest that the lymphatic system may play an important role in arthritis pathogenesis and treatment.
Url:
DOI: 10.3109/10520295.2012.729864
PubMed: 23173750
PubMed Central: 3672261
Affiliations:
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Shi</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Orthopaedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Orthopaedics, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062</wicri:regionArea><wicri:noRegion>Shanghai 200062</wicri:noRegion></affiliation><affiliation wicri:level="2"><nlm:aff id="A2">Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation></author><author><name sortKey="Liang, Q" sort="Liang, Q" uniqKey="Liang Q" first="Q" last="Liang">Q. Liang</name><affiliation wicri:level="2"><nlm:aff id="A2">Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Y" sort="Wang, Y" uniqKey="Wang Y" first="Y" last="Wang">Y. Wang</name><affiliation wicri:level="1"><nlm:aff id="A3">Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Spine, Shanghai University of Traditional Chinese Medicine, Shanghai, 200032</wicri:regionArea><wicri:noRegion>200032</wicri:noRegion></affiliation></author><author><name sortKey="Mooney, Ra" sort="Mooney, Ra" uniqKey="Mooney R" first="Ra" last="Mooney">Ra Mooney</name><affiliation wicri:level="2"><nlm:aff id="A2">Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="A4">Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Boyce, Bf" sort="Boyce, Bf" uniqKey="Boyce B" first="Bf" last="Boyce">Bf Boyce</name><affiliation wicri:level="2"><nlm:aff id="A2">Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="A4">Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author><author><name sortKey="Xing, L" sort="Xing, L" uniqKey="Xing L" first="L" last="Xing">L. Xing</name><affiliation wicri:level="2"><nlm:aff id="A2">Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="A4">Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, New York</wicri:regionArea><placeName><region type="state">État de New York</region></placeName></affiliation></author></analytic><series><title level="j">Biotechnic & histochemistry : official publication of the Biological Stain Commission</title><idno type="ISSN">1052-0295</idno><idno type="eISSN">1473-7760</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P2">We investigated the presence and alteration of lymphatic vessels in joints of arthritic mice using a whole-slide imaging system. Joints and long bone sections were cut from paraffin blocks of two mouse models of arthritis: meniscal-ligamentous injury (MLI)-induced osteoarthritis (OA) and TNF transgene (TNF-Tg)-induced rheumatoid arthritis (RA). MLI-OA mice were fed a high fat diet to accelerate OA development. TNF-Tg mice were treated with lymphatic growth factor VEGF-C virus to stimulate lymphangiogenesis. Sections were double immunofluorescence stained with anti-podoplanin and alpha-smooth muscle action. The area and number of lymphatic capillaries and mature lymphatic vessels were determined using a whole-slide imaging system and its associated software. Lymphatic vessels in joints were distributed in soft tissues mainly around the joint capsule, ligaments, fat pads and muscles. In long bones, enriched lymphatic vessels were present in the periosteal areas adjacent to the blood vessels. Occasionally, lymphatic vessels were observed in the cortical bone. Increased lymphatic capillaries, but decreased mature lymphatic vessels, were detected in both OA and RA joints. VEGF-C treatment increased lymphatic capillary and mature vessel formation in RA joints. We demonstrated decreased mature lymphatic vessels in the joints of mouse models of severe OA and RA. VEGF-C treatment increased the lymphatic vessel number and area in RA joints. Our findings suggest that the lymphatic system may play an important role in arthritis pathogenesis and treatment.</p></div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Shi, J" sort="Shi, J" uniqKey="Shi J" first="J" last="Shi">J. Shi</name></noRegion><name sortKey="Liang, Q" sort="Liang, Q" uniqKey="Liang Q" first="Q" last="Liang">Q. Liang</name><name sortKey="Shi, J" sort="Shi, J" uniqKey="Shi J" first="J" last="Shi">J. Shi</name><name sortKey="Wang, Y" sort="Wang, Y" uniqKey="Wang Y" first="Y" last="Wang">Y. Wang</name></country><country name="États-Unis"><region name="État de New York"><name sortKey="Shi, J" sort="Shi, J" uniqKey="Shi J" first="J" last="Shi">J. Shi</name></region><name sortKey="Boyce, Bf" sort="Boyce, Bf" uniqKey="Boyce B" first="Bf" last="Boyce">Bf Boyce</name><name sortKey="Boyce, Bf" sort="Boyce, Bf" uniqKey="Boyce B" first="Bf" last="Boyce">Bf Boyce</name><name sortKey="Liang, Q" sort="Liang, Q" uniqKey="Liang Q" first="Q" last="Liang">Q. Liang</name><name sortKey="Mooney, Ra" sort="Mooney, Ra" uniqKey="Mooney R" first="Ra" last="Mooney">Ra Mooney</name><name sortKey="Mooney, Ra" sort="Mooney, Ra" uniqKey="Mooney R" first="Ra" last="Mooney">Ra Mooney</name><name sortKey="Xing, L" sort="Xing, L" uniqKey="Xing L" first="L" last="Xing">L. Xing</name><name sortKey="Xing, L" sort="Xing, L" uniqKey="Xing L" first="L" last="Xing">L. 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