An in Vivo Method for Visualizing Flow Dynamics of Cells within Corneal Lymphatics
Identifieur interne : 003894 ( Main/Exploration ); précédent : 003893; suivant : 003895An in Vivo Method for Visualizing Flow Dynamics of Cells within Corneal Lymphatics
Auteurs : Beatrice Bourghardt Peebo ; Per Fagerholm ; Neil LagaliSource :
- Lymphatic Research and Biology [ 1539-6851 ] ; 2013.
Abstract
Monitoring the trafficking of specific cell populations within lymphatics could improve our understanding of processes such as transplant rejection and cancer metastasis. Current methods, however, lack appropriate image resolution for single-cell analysis or are incompatible with
Inflammatory angiogenesis was induced by suture placement in corneas of Wistar rats. Pre- and up to 3 weeks post-induction, corneas were noninvasively examined by laser-scanning
The
Url:
DOI: 10.1089/lrb.2012.0023
PubMed: 23772718
PubMed Central: 3685390
Affiliations:
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Le document en format XML
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Method for Visualizing Flow Dynamics of Cells within Corneal Lymphatics</title>
<author><name sortKey="Peebo, Beatrice Bourghardt" sort="Peebo, Beatrice Bourghardt" uniqKey="Peebo B" first="Beatrice Bourghardt" last="Peebo">Beatrice Bourghardt Peebo</name>
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<author><name sortKey="Fagerholm, Per" sort="Fagerholm, Per" uniqKey="Fagerholm P" first="Per" last="Fagerholm">Per Fagerholm</name>
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<author><name sortKey="Lagali, Neil" sort="Lagali, Neil" uniqKey="Lagali N" first="Neil" last="Lagali">Neil Lagali</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">An <italic>in Vivo</italic>
Method for Visualizing Flow Dynamics of Cells within Corneal Lymphatics</title>
<author><name sortKey="Peebo, Beatrice Bourghardt" sort="Peebo, Beatrice Bourghardt" uniqKey="Peebo B" first="Beatrice Bourghardt" last="Peebo">Beatrice Bourghardt Peebo</name>
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<author><name sortKey="Fagerholm, Per" sort="Fagerholm, Per" uniqKey="Fagerholm P" first="Per" last="Fagerholm">Per Fagerholm</name>
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<author><name sortKey="Lagali, Neil" sort="Lagali, Neil" uniqKey="Lagali N" first="Neil" last="Lagali">Neil Lagali</name>
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<series><title level="j">Lymphatic Research and Biology</title>
<idno type="ISSN">1539-6851</idno>
<idno type="eISSN">1557-8585</idno>
<imprint><date when="2013">2013</date>
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<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<sec><title>Background</title>
<p>Monitoring the trafficking of specific cell populations within lymphatics could improve our understanding of processes such as transplant rejection and cancer metastasis. Current methods, however, lack appropriate image resolution for single-cell analysis or are incompatible with <italic>in vivo</italic>
and longitudinal monitoring of lymphatics in their native state. We therefore sought to achieve high-resolution live imaging of the dynamic behavior of cells within lymph vessels in the rat cornea.</p>
</sec>
<sec><title>Methods/Results</title>
<p>Inflammatory angiogenesis was induced by suture placement in corneas of Wistar rats. Pre- and up to 3 weeks post-induction, corneas were noninvasively examined by laser-scanning <italic>in vivo</italic>
corneal confocal microscopy (IVCM) using only endogenous contrast. Lymph vessels and the cells harbored therein were documented by still images, real-time video, and 3D confocal stack reconstruction of live tissue. <italic>In vivo,</italic>
conjunctival and corneal lymphatics were morphologically distinct, those with corneal location being one-quarter the diameter of those in the conjunctiva (<italic>p</italic>
<0.001). Cells were recruited to initially empty pre-existing lymph vessels during the first day of inflammation and maintained a dense occupation of vessels for up to 7 days. A diverse population of cells (diameter range: 1.5–27.5 μm) with varying morphology was observed, and exhibited variable flow patterns and were transported singly and in clusters of at least 2–9 adherent cells.</p>
</sec>
<sec><title>Conclusions</title>
<p>The <italic>in vivo</italic>
microscopic technique presented enables lymph vessels and cell trafficking to be studied in high resolution in a minimally-perturbed physiologic milieu.</p>
</sec>
</div>
</front>
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<tree><noCountry><name sortKey="Fagerholm, Per" sort="Fagerholm, Per" uniqKey="Fagerholm P" first="Per" last="Fagerholm">Per Fagerholm</name>
<name sortKey="Lagali, Neil" sort="Lagali, Neil" uniqKey="Lagali N" first="Neil" last="Lagali">Neil Lagali</name>
<name sortKey="Peebo, Beatrice Bourghardt" sort="Peebo, Beatrice Bourghardt" uniqKey="Peebo B" first="Beatrice Bourghardt" last="Peebo">Beatrice Bourghardt Peebo</name>
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