EphB4 forward signalling regulates lymphatic valve development
Identifieur interne : 001E30 ( Main/Exploration ); précédent : 001E29; suivant : 001E31EphB4 forward signalling regulates lymphatic valve development
Auteurs : Gu Zhang [États-Unis] ; John Brady [États-Unis] ; Wei-Ching Liang [États-Unis] ; Yan Wu [États-Unis] ; Mark Henkemeyer [États-Unis] ; Minhong Yan [États-Unis]Source :
- Nature Communications [ 2041-1723 ] ; 2015.
Abstract
Bidirectional signalling is regarded as a notable hallmark of the Eph-ephrin signalling system: Eph-dependent forward signalling in Eph-expressing cells and ephrin-dependent reverse signalling in Ephrin-expressing cells. The notion of ephrin-dependent reverse signalling derives from genetic experiments utilizing mice carrying mutations in the intracellular region of ephrinBs. Here we show that EphB4-dependent forward signalling regulates lymphatic valve development, a process previously thought to be regulated by ephrinB2-dependent reverse signalling. We develop antibodies that selectively target EphB4 and ephrinB2. We find that mice bearing genetically altered cytoplasmic region of ephrinB2 have significantly altered EphB4-dependent forward signalling. Selective inhibition of EphB4 using a functional blocking antibody results in defective lymphatic valve development. Furthermore, a chemical genetic approach is used to unequivocally show that the kinase activity of EphB4 is essential for lymphatic valve development.
Url:
DOI: 10.1038/ncomms7625
PubMed: 25865237
PubMed Central: 4403310
Affiliations:
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Liang</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Zhang, Gu" sort="Zhang, Gu" uniqKey="Zhang G" first="Gu" last="Zhang">Gu Zhang</name></noRegion><name sortKey="Brady, John" sort="Brady, John" uniqKey="Brady J" first="John" last="Brady">John Brady</name><name sortKey="Henkemeyer, Mark" sort="Henkemeyer, Mark" uniqKey="Henkemeyer M" first="Mark" last="Henkemeyer">Mark Henkemeyer</name><name sortKey="Liang, Wei Ching" sort="Liang, Wei Ching" uniqKey="Liang W" first="Wei-Ching" last="Liang">Wei-Ching Liang</name><name sortKey="Wu, Yan" sort="Wu, Yan" uniqKey="Wu Y" first="Yan" last="Wu">Yan Wu</name><name sortKey="Yan, Minhong" sort="Yan, Minhong" uniqKey="Yan M" first="Minhong" last="Yan">Minhong Yan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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