Enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection using urine samples and its application in Bangladesh.
Identifieur interne : 003667 ( Main/Curation ); précédent : 003666; suivant : 003668Enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection using urine samples and its application in Bangladesh.
Auteurs : Mohammad Sohel Samad [Japon] ; Makoto Itoh ; Kazuhiko Moji ; Moazzem Hossain ; Dinesh Mondal ; Mohammad Shafiul Alam ; Eisaku KimuraSource :
- Parasitology international [ 1873-0329 ] ; 2013.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Animaux, Anticorps antihelminthe (immunologie), Antigènes d'helminthe (immunologie), Bangladesh (épidémiologie), Enfant, Enfant d'âge préscolaire, Femelle, Filariose lymphatique (diagnostic), Filariose lymphatique (urine), Filariose lymphatique (épidémiologie), Humains, Immunoglobuline G (urine), Jeune adulte, Mâle, Protéines recombinantes, Sensibilité et spécificité, Sujet âgé, Test ELISA (), Wuchereria bancrofti (immunologie), Wuchereria bancrofti (isolement et purification), Études transversales.
- MESH :
- diagnostic : Filariose lymphatique.
- immunologie : Anticorps antihelminthe, Antigènes d'helminthe, Wuchereria bancrofti.
- isolement et purification : Wuchereria bancrofti.
- épidémiologie : Bangladesh, Filariose lymphatique, Immunoglobuline G.
- Adulte, Adulte d'âge moyen, Animaux, Enfant, Enfant d'âge préscolaire, Femelle, Humains, Jeune adulte, Mâle, Protéines recombinantes, Sensibilité et spécificité, Sujet âgé, Test ELISA, Études transversales.
- Wicri :
- geographic : Bangladesh.
English descriptors
- KwdEn :
- Adult, Aged, Animals, Antibodies, Helminth (immunology), Antigens, Helminth (immunology), Bangladesh (epidemiology), Child, Child, Preschool, Cross-Sectional Studies, Elephantiasis, Filarial (diagnosis), Elephantiasis, Filarial (epidemiology), Elephantiasis, Filarial (urine), Enzyme-Linked Immunosorbent Assay (methods), Female, Humans, Immunoglobulin G (urine), Male, Middle Aged, Recombinant Proteins, Sensitivity and Specificity, Wuchereria bancrofti (immunology), Wuchereria bancrofti (isolation & purification), Young Adult.
- MESH :
- chemical , immunology : Antibodies, Helminth, Antigens, Helminth.
- geographic , epidemiology : Bangladesh.
- diagnosis : Elephantiasis, Filarial.
- epidemiology : Elephantiasis, Filarial.
- immunology : Wuchereria bancrofti.
- isolation & purification : Wuchereria bancrofti.
- methods : Enzyme-Linked Immunosorbent Assay.
- urine : Elephantiasis, Filarial, Immunoglobulin G.
- Adult, Aged, Animals, Child, Child, Preschool, Cross-Sectional Studies, Female, Humans, Male, Middle Aged, Recombinant Proteins, Sensitivity and Specificity, Young Adult.
Abstract
In Sri Lanka, urine ELISA showed high sensitivity and specificity in detecting filaria-specific IgG4. It also produced much higher positive rates than antigen tests in prevalence studies with young children. In this study, we have confirmed the usefulness of urine ELISA in the field of Bangladesh. The ELISA detected 89 of 105 (85%) ICT antigen test positive subjects in endemic areas. With both ICT and microfilaria positives, the sensitivity was 97% (30/31). All of 104 ICT negative people in a non-endemic area were ELISA negative (100% specificity). In a prevalence study with 319 young children (5-10 years) from a low endemic area after five rounds of MDA, seven (2.2%) were detected by the present urine test, but only one (0.3%) by ICT (P=0.075). The satisfactorily high sensitivity, 100% specificity and effective case detection among young ages along with scope for analyzing the titers will indicate urine ELISA to be an effective tool in the post-MDA surveys to confirm elimination or to detect resurgence in Bangladesh.
DOI: 10.1016/j.parint.2013.08.007
PubMed: 23988626
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pubmed:23988626Le document en format XML
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Specificity</term><term>Young Adult</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Animaux</term><term>Enfant</term><term>Enfant d'âge préscolaire</term><term>Femelle</term><term>Humains</term><term>Jeune adulte</term><term>Mâle</term><term>Protéines recombinantes</term><term>Sensibilité et spécificité</term><term>Sujet âgé</term><term>Test ELISA</term><term>Études transversales</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Bangladesh</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In Sri Lanka, urine ELISA showed high sensitivity and specificity in detecting filaria-specific IgG4. It also produced much higher positive rates than antigen tests in prevalence studies with young children. In this study, we have confirmed the usefulness of urine ELISA in the field of Bangladesh. The ELISA detected 89 of 105 (85%) ICT antigen test positive subjects in endemic areas. With both ICT and microfilaria positives, the sensitivity was 97% (30/31). All of 104 ICT negative people in a non-endemic area were ELISA negative (100% specificity). In a prevalence study with 319 young children (5-10 years) from a low endemic area after five rounds of MDA, seven (2.2%) were detected by the present urine test, but only one (0.3%) by ICT (P=0.075). The satisfactorily high sensitivity, 100% specificity and effective case detection among young ages along with scope for analyzing the titers will indicate urine ELISA to be an effective tool in the post-MDA surveys to confirm elimination or to detect resurgence in Bangladesh.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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