Intraoperative Examination of Sentinel Lymph Nodes by Ultrarapid Immunohistochemistry in Breast Cancer
Identifieur interne : 004D23 ( Istex/Curation ); précédent : 004D22; suivant : 004D24Intraoperative Examination of Sentinel Lymph Nodes by Ultrarapid Immunohistochemistry in Breast Cancer
Auteurs : Young Jin Choi ; Hae Ran Yun ; Ki Eun Yoo ; Jung Han Kim ; Seok Jin Nam ; Yoon La Choi ; Young Hyeh Ko ; Byung Tae Kim [Corée du Sud] ; Jung-Hyun YangSource :
- Japanese Journal of Clinical Oncology [ 0368-2811 ] ; 2006-08.
English descriptors
Abstract
Background: The ultrarapid immunohistochemistry (IHC) technique was applied to the intraoperative examination of sentinel lymph nodes (SLNs) because routine SLN frozen section examinations sometimes produce false-negative results. The present study was undertaken to develop a reliable protocol for the ultrarapid IHC of SLNs. Methods: SLNs from 79 breast cancer patients with clinically negative axillary node were examined intraoperatively by frozen hematoxylin–eosin (H&E) stain and by ultrarapid cytokeratin IHC assay. On the basis of the result of serially sectioned permanent study, the sensitivity and accuracy of each intraoperative technique were compared. Results: The total number of dissected SLNs was 178 with a mean of 2.3 (1–5) per patient. The mean turnaround time for ultrarapid IHC was 20 min. The sensitivity rates of frozen H&E staining and ultrarapid IHC were 70.0 and 85.0%, respectively (P = 0.083). Each method had a specificity of 100%. The accuracy rates for frozen H&E staining and rapid IHC were 92.4 and 96.2%, respectively (P = 0.083). Ultrarapid IHC detected one additional patient with sentinel node micrometastasis and two additional patients with isolated tumor cells (ITCs). In those patients, two underwent completion axillary dissection simultaneously and could avoid a second operation. Conclusions: Ultrarapid cytokeratin IHC enhanced the intraoperative detection of sentinel node micrometastasis and ITCs in breast cancer without consuming much time. In patients who need completion axillary dissection after sentinel node biopsy, this technique could be helpful in avoiding a second operation.
Url:
DOI: 10.1093/jjco/hyl045
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :004D23
Links to Exploration step
ISTEX:A44D271A98F9C007DB6CA407F508CAFFB19D83DCCuration
No country items
Young Jin Choi<affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation>Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Intraoperative Examination of Sentinel Lymph Nodes by Ultrarapid Immunohistochemistry in Breast Cancer</title><author><name sortKey="Choi, Young Jin" sort="Choi, Young Jin" uniqKey="Choi Y" first="Young Jin" last="Choi">Young Jin Choi</name><affiliation><mods:affiliation>Surgery,</mods:affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Yun, Hae Ran" sort="Yun, Hae Ran" uniqKey="Yun H" first="Hae Ran" last="Yun">Hae Ran Yun</name><affiliation><mods:affiliation>Surgery,</mods:affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Yoo, Ki Eun" sort="Yoo, Ki Eun" uniqKey="Yoo K" first="Ki Eun" last="Yoo">Ki Eun Yoo</name><affiliation><mods:affiliation>Surgery,</mods:affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Kim, Jung Han" sort="Kim, Jung Han" uniqKey="Kim J" first="Jung Han" last="Kim">Jung Han Kim</name><affiliation><mods:affiliation>Surgery,</mods:affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Nam, Seok Jin" sort="Nam, Seok Jin" uniqKey="Nam S" first="Seok Jin" last="Nam">Seok Jin Nam</name><affiliation><mods:affiliation>Surgery,</mods:affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Choi, Yoon La" sort="Choi, Yoon La" uniqKey="Choi Y" first="Yoon La" last="Choi">Yoon La Choi</name><affiliation><mods:affiliation>Pathology and</mods:affiliation><wicri:noCountry code="no comma">Pathology and</wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Ko, Young Hyeh" sort="Ko, Young Hyeh" uniqKey="Ko Y" first="Young Hyeh" last="Ko">Young Hyeh Ko</name><affiliation><mods:affiliation>Pathology and</mods:affiliation><wicri:noCountry code="no comma">Pathology and</wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Kim, Byung Tae" sort="Kim, Byung Tae" uniqKey="Kim B" first="Byung Tae" last="Kim">Byung Tae Kim</name><affiliation wicri:level="1"><mods:affiliation>Nuclear Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea</mods:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Nuclear Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul</wicri:regionArea></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Yang, Jung Hyun" sort="Yang, Jung Hyun" uniqKey="Yang J" first="Jung-Hyun" last="Yang">Jung-Hyun Yang</name><affiliation><mods:affiliation>Surgery,</mods:affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A44D271A98F9C007DB6CA407F508CAFFB19D83DC</idno><date when="2006" year="2006">2006</date><idno type="doi">10.1093/jjco/hyl045</idno><idno type="url">https://api.istex.fr/document/A44D271A98F9C007DB6CA407F508CAFFB19D83DC/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">004D23</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">004D23</idno><idno type="wicri:Area/Istex/Curation">004D23</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Intraoperative Examination of Sentinel Lymph Nodes by Ultrarapid Immunohistochemistry in Breast Cancer</title><author><name sortKey="Choi, Young Jin" sort="Choi, Young Jin" uniqKey="Choi Y" first="Young Jin" last="Choi">Young Jin Choi</name><affiliation><mods:affiliation>Surgery,</mods:affiliation></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Yun, Hae Ran" sort="Yun, Hae Ran" uniqKey="Yun H" first="Hae Ran" last="Yun">Hae Ran Yun</name><affiliation><mods:affiliation>Surgery,</mods:affiliation></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Yoo, Ki Eun" sort="Yoo, Ki Eun" uniqKey="Yoo K" first="Ki Eun" last="Yoo">Ki Eun Yoo</name><affiliation><mods:affiliation>Surgery,</mods:affiliation></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Kim, Jung Han" sort="Kim, Jung Han" uniqKey="Kim J" first="Jung Han" last="Kim">Jung Han Kim</name><affiliation><mods:affiliation>Surgery,</mods:affiliation></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Nam, Seok Jin" sort="Nam, Seok Jin" uniqKey="Nam S" first="Seok Jin" last="Nam">Seok Jin Nam</name><affiliation><mods:affiliation>Surgery,</mods:affiliation></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Choi, Yoon La" sort="Choi, Yoon La" uniqKey="Choi Y" first="Yoon La" last="Choi">Yoon La Choi</name><affiliation><mods:affiliation>Pathology and</mods:affiliation></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Ko, Young Hyeh" sort="Ko, Young Hyeh" uniqKey="Ko Y" first="Young Hyeh" last="Ko">Young Hyeh Ko</name><affiliation><mods:affiliation>Pathology and</mods:affiliation></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author><author><name sortKey="Kim, Byung Tae" sort="Kim, Byung Tae" uniqKey="Kim B" first="Byung Tae" last="Kim">Byung Tae Kim</name><affiliation wicri:level="1"><mods:affiliation>Nuclear Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea</mods:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Nuclear Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul</wicri:regionArea></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation></affiliation></author><author><name sortKey="Yang, Jung Hyun" sort="Yang, Jung Hyun" uniqKey="Yang J" first="Jung-Hyun" last="Yang">Jung-Hyun Yang</name><affiliation><mods:affiliation>Surgery,</mods:affiliation></affiliation><affiliation><mods:affiliation>Departments of</mods:affiliation><wicri:noCountry code="no comma">Departments of</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j">Japanese Journal of Clinical Oncology</title><title level="j" type="abbrev">JJCO</title><idno type="ISSN">0368-2811</idno><idno type="eISSN">1465-3621</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2006-08">2006-08</date><biblScope unit="volume">36</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="489">489</biblScope><biblScope unit="page" to="493">493</biblScope></imprint><idno type="ISSN">0368-2811</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0368-2811</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>breast cancer</term><term>micrometastasis</term><term>sentinel lymph node</term><term>ultrarapid immunohistochemistry (IHC)</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Background: The ultrarapid immunohistochemistry (IHC) technique was applied to the intraoperative examination of sentinel lymph nodes (SLNs) because routine SLN frozen section examinations sometimes produce false-negative results. The present study was undertaken to develop a reliable protocol for the ultrarapid IHC of SLNs. Methods: SLNs from 79 breast cancer patients with clinically negative axillary node were examined intraoperatively by frozen hematoxylin–eosin (H&E) stain and by ultrarapid cytokeratin IHC assay. On the basis of the result of serially sectioned permanent study, the sensitivity and accuracy of each intraoperative technique were compared. Results: The total number of dissected SLNs was 178 with a mean of 2.3 (1–5) per patient. The mean turnaround time for ultrarapid IHC was 20 min. The sensitivity rates of frozen H&E staining and ultrarapid IHC were 70.0 and 85.0%, respectively (P = 0.083). Each method had a specificity of 100%. The accuracy rates for frozen H&E staining and rapid IHC were 92.4 and 96.2%, respectively (P = 0.083). Ultrarapid IHC detected one additional patient with sentinel node micrometastasis and two additional patients with isolated tumor cells (ITCs). In those patients, two underwent completion axillary dissection simultaneously and could avoid a second operation. Conclusions: Ultrarapid cytokeratin IHC enhanced the intraoperative detection of sentinel node micrometastasis and ITCs in breast cancer without consuming much time. In patients who need completion axillary dissection after sentinel node biopsy, this technique could be helpful in avoiding a second operation.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004D23 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 004D23 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:A44D271A98F9C007DB6CA407F508CAFFB19D83DC
|texte= Intraoperative Examination of Sentinel Lymph Nodes by Ultrarapid Immunohistochemistry in Breast Cancer
}}
| This area was generated with Dilib version V0.6.31. |  |