A filter paper technique for the detection of anti-filarial IgG4 in lymphatic filariasis
Identifieur interne : 004E20 ( Istex/Corpus ); précédent : 004E19; suivant : 004E21A filter paper technique for the detection of anti-filarial IgG4 in lymphatic filariasis
Auteurs : Annemarie J. Terhell ; Melang Haarbrink ; Kunar Abadi ; Daniëlle C. H. Bronneberg ; Marjo C. Tieleman ; Muhammad Asri ; Maria YazdanbakhshSource :
- Transactions of the Royal Society of Tropical Medicine and Hygiene [ 0035-9203 ] ; 1996.
Abstract
In a previous study performed in south Sulawesi (Sulawesi Selatan), Indonesia, we established that the immunoglobulin 64 (IgG4) enzyme-linked immunosorbent assay (elisa) is a suitable community diagnostic method and that it can distinguish areas of high and low prevelances within short distances. In an attempt to make this diagnostic tool more applicable in the field, a comparative study using serum and blood collected on filter paper was undertaken with 568 individuals living in 2 areas with different endemicity for brugian filariasis in south Sulawesi. In Mamuju district, where the microfilaria (mf) prevalence of the studied individuals was 18·4%, antifilarial IgG4 was present in 73·1% of the venepuncture samples and 72·5% of the filter paper samples, respectively. In Mangkutane district, where lymphatic filariasis is transmitted at a low level (mf rate 2·4%), antifilarial IgG4 was detected in 35·5% and 39·9% of similar samples, respectively. There was no significant difference in the IgG4 detection rate determined from venepuncture and filter paper samples from the same donors (P = 0·124), and the IgG4 values were highly correlated (ν = 0·97, P < 0·001, n = 568). These results indicate that the filter paper technique for collection of blood samples is a suitable alternative to venepuncture for use in the IgG4 elisa.
Url:
DOI: 10.1016/S0035-9203(96)90140-6
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Terhell</name><affiliation><mods:affiliation>Department of Parasitology, Leiden University, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Haarbrink, Melang" sort="Haarbrink, Melang" uniqKey="Haarbrink M" first="Melang" last="Haarbrink">Melang Haarbrink</name><affiliation><mods:affiliation>Department of Parasitology, Leiden University, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Abadi, Kunar" sort="Abadi, Kunar" uniqKey="Abadi K" first="Kunar" last="Abadi">Kunar Abadi</name><affiliation><mods:affiliation>Department of Parasitology, Hasanuddin University, Ujung Pandang, Indonesia</mods:affiliation></affiliation></author><author><name sortKey="Bronneberg, Danielle C H" sort="Bronneberg, Danielle C H" uniqKey="Bronneberg D" first="Daniëlle C. H." last="Bronneberg">Daniëlle C. H. 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Terhell</name><affiliation><mods:affiliation>Department of Parasitology, Leiden University, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Haarbrink, Melang" sort="Haarbrink, Melang" uniqKey="Haarbrink M" first="Melang" last="Haarbrink">Melang Haarbrink</name><affiliation><mods:affiliation>Department of Parasitology, Leiden University, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Abadi, Kunar" sort="Abadi, Kunar" uniqKey="Abadi K" first="Kunar" last="Abadi">Kunar Abadi</name><affiliation><mods:affiliation>Department of Parasitology, Hasanuddin University, Ujung Pandang, Indonesia</mods:affiliation></affiliation></author><author><name sortKey="Bronneberg, Danielle C H" sort="Bronneberg, Danielle C H" uniqKey="Bronneberg D" first="Daniëlle C. H." last="Bronneberg">Daniëlle C. H. Bronneberg</name><affiliation><mods:affiliation>Department of Parasitology, Leiden University, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Tieleman, Marjo C" sort="Tieleman, Marjo C" uniqKey="Tieleman M" first="Marjo C." last="Tieleman">Marjo C. Tieleman</name><affiliation><mods:affiliation>Department of Parasitology, Leiden University, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Asri, Muhammad" sort="Asri, Muhammad" uniqKey="Asri M" first="Muhammad" last="Asri">Muhammad Asri</name><affiliation><mods:affiliation>Communicable Disease Centre (CDC), Ujung Pandang, Indonesia</mods:affiliation></affiliation></author><author><name sortKey="Yazdanbakhsh, Maria" sort="Yazdanbakhsh, Maria" uniqKey="Yazdanbakhsh M" first="Maria" last="Yazdanbakhsh">Maria Yazdanbakhsh</name><affiliation><mods:affiliation>Address for correspondence: Dr Maria Yazdanbakhsh, Department of Parasitology, Leiden University, Wassenaarseweg 62, Postbus 9605, 2300 RC Leiden, The Netherlands; fax +31 71 5276850.</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Parasitology, Leiden University, The Netherlands</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: maria@rullf2.leidenuniv.nl</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Transactions of the Royal Society of Tropical Medicine and Hygiene</title><title level="j" type="abbrev">TRSTMH</title><idno type="ISSN">0035-9203</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">90</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="196">196</biblScope><biblScope unit="page" to="198">198</biblScope></imprint><idno type="ISSN">0035-9203</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0035-9203</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In a previous study performed in south Sulawesi (Sulawesi Selatan), Indonesia, we established that the immunoglobulin 64 (IgG4) enzyme-linked immunosorbent assay (elisa) is a suitable community diagnostic method and that it can distinguish areas of high and low prevelances within short distances. In an attempt to make this diagnostic tool more applicable in the field, a comparative study using serum and blood collected on filter paper was undertaken with 568 individuals living in 2 areas with different endemicity for brugian filariasis in south Sulawesi. In Mamuju district, where the microfilaria (mf) prevalence of the studied individuals was 18·4%, antifilarial IgG4 was present in 73·1% of the venepuncture samples and 72·5% of the filter paper samples, respectively. In Mangkutane district, where lymphatic filariasis is transmitted at a low level (mf rate 2·4%), antifilarial IgG4 was detected in 35·5% and 39·9% of similar samples, respectively. There was no significant difference in the IgG4 detection rate determined from venepuncture and filter paper samples from the same donors (P = 0·124), and the IgG4 values were highly correlated (ν = 0·97, P < 0·001, n = 568). These results indicate that the filter paper technique for collection of blood samples is a suitable alternative to venepuncture for use in the IgG4 elisa.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>igg4</json:string><json:string>mangkutane</json:string><json:string>filariasis</json:string><json:string>venepuncture</json:string><json:string>mamuju</json:string><json:string>antifilarial</json:string><json:string>filter paper</json:string><json:string>elisa</json:string><json:string>antifilarial igg4</json:string><json:string>ujung pandang</json:string><json:string>pandang</json:string><json:string>ujung</json:string><json:string>parasitological</json:string><json:string>inhabitant</json:string><json:string>yazdanbakhsh</json:string><json:string>sulawesi</json:string><json:string>endemic</json:string><json:string>elephantiasis</json:string><json:string>filter paper samples</json:string><json:string>parasitology</json:string><json:string>microfilariae</json:string><json:string>lymphatic</json:string><json:string>endemicity</json:string><json:string>microfilaria</json:string><json:string>immunosorbent</json:string><json:string>kurniawan</json:string><json:string>filarial</json:string><json:string>mamuju district</json:string><json:string>infectious diseases</json:string><json:string>blood samples</json:string><json:string>same individuals</json:string><json:string>venous blood</json:string><json:string>igg4 elisa</json:string><json:string>present study</json:string><json:string>mangkutane district</json:string><json:string>tropical medicine</json:string><json:string>specific igg4</json:string><json:string>significant difference</json:string><json:string>leiden university</json:string><json:string>lymphatic filariasis</json:string><json:string>brugian filariasis</json:string><json:string>venepuncture blood</json:string><json:string>major problem</json:string><json:string>sulawesi selatan</json:string><json:string>previous study</json:string><json:string>hasanuddin university</json:string><json:string>suitable alternative</json:string><json:string>final concentration</json:string><json:string>several months</json:string><json:string>maria yazdanbakhsh</json:string><json:string>parasitological examination</json:string><json:string>elephantiasis patients</json:string><json:string>igg4 antibodies</json:string><json:string>venepuncture samples</json:string><json:string>leiden</json:string><json:string>assay</json:string></teeft></keywords><author><json:item><name>Annemarie J. Terhell</name><affiliations><json:string>Department of Parasitology, Leiden University, The Netherlands</json:string></affiliations></json:item><json:item><name>Melang Haarbrink</name><affiliations><json:string>Department of Parasitology, Leiden University, The Netherlands</json:string></affiliations></json:item><json:item><name>Kunar Abadi</name><affiliations><json:string>Department of Parasitology, Hasanuddin University, Ujung Pandang, Indonesia</json:string></affiliations></json:item><json:item><name>Daniëlle C.H. Bronneberg</name><affiliations><json:string>Department of Parasitology, Leiden University, The Netherlands</json:string></affiliations></json:item><json:item><name>Marjo C. Tieleman</name><affiliations><json:string>Department of Parasitology, Leiden University, The Netherlands</json:string></affiliations></json:item><json:item><name>Muhammad Asri</name><affiliations><json:string>Communicable Disease Centre (CDC), Ujung Pandang, Indonesia</json:string></affiliations></json:item><json:item><name>Maria Yazdanbakhsh</name><affiliations><json:string>Address for correspondence: Dr Maria Yazdanbakhsh, Department of Parasitology, Leiden University, Wassenaarseweg 62, Postbus 9605, 2300 RC Leiden, The Netherlands; fax +31 71 5276850.</json:string><json:string>Department of Parasitology, Leiden University, The Netherlands</json:string><json:string>E-mail: maria@rullf2.leidenuniv.nl</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Immunobiology</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>filariasis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Brugia malayi</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>IgG4</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>enzyme-linked immunosorbent assay</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Short communication</json:string></originalGenre><abstract>In a previous study performed in south Sulawesi (Sulawesi Selatan), Indonesia, we established that the immunoglobulin 64 (IgG4) enzyme-linked immunosorbent assay (elisa) is a suitable community diagnostic method and that it can distinguish areas of high and low prevelances within short distances. In an attempt to make this diagnostic tool more applicable in the field, a comparative study using serum and blood collected on filter paper was undertaken with 568 individuals living in 2 areas with different endemicity for brugian filariasis in south Sulawesi. In Mamuju district, where the microfilaria (mf) prevalence of the studied individuals was 18·4%, antifilarial IgG4 was present in 73·1% of the venepuncture samples and 72·5% of the filter paper samples, respectively. In Mangkutane district, where lymphatic filariasis is transmitted at a low level (mf rate 2·4%), antifilarial IgG4 was detected in 35·5% and 39·9% of similar samples, respectively. There was no significant difference in the IgG4 detection rate determined from venepuncture and filter paper samples from the same donors (P = 0·124), and the IgG4 values were highly correlated (ν = 0·97, P > 0·001, n = 568). 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fax +31 71 5276850.</affiliation><affiliation>Department of Parasitology, Leiden University, The Netherlands</affiliation></author><idno type="istex">A6451A7D37F50594887F718B6EA74C3CB9DB73BA</idno><idno type="DOI">10.1016/S0035-9203(96)90140-6</idno><idno type="PII">S0035-9203(96)90140-6</idno></analytic><monogr><title level="j">Transactions of the Royal Society of Tropical Medicine and Hygiene</title><title level="j" type="abbrev">TRSTMH</title><idno type="pISSN">0035-9203</idno><idno type="PII">S0035-9203(00)X0178-2</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996"></date><biblScope unit="volume">90</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="196">196</biblScope><biblScope unit="page" to="198">198</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1996</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>In a previous study performed in south Sulawesi (Sulawesi Selatan), Indonesia, we established that the immunoglobulin 64 (IgG4) enzyme-linked immunosorbent assay (elisa) is a suitable community diagnostic method and that it can distinguish areas of high and low prevelances within short distances. In an attempt to make this diagnostic tool more applicable in the field, a comparative study using serum and blood collected on filter paper was undertaken with 568 individuals living in 2 areas with different endemicity for brugian filariasis in south Sulawesi. In Mamuju district, where the microfilaria (mf) prevalence of the studied individuals was 18·4%, antifilarial IgG4 was present in 73·1% of the venepuncture samples and 72·5% of the filter paper samples, respectively. In Mangkutane district, where lymphatic filariasis is transmitted at a low level (mf rate 2·4%), antifilarial IgG4 was detected in 35·5% and 39·9% of similar samples, respectively. There was no significant difference in the IgG4 detection rate determined from venepuncture and filter paper samples from the same donors (P = 0·124), and the IgG4 values were highly correlated (ν = 0·97, P < 0·001, n = 568). These results indicate that the filter paper technique for collection of blood samples is a suitable alternative to venepuncture for use in the IgG4 elisa.</p></abstract><textClass><keywords scheme="keyword"><list><head>article-category</head><item><term>Immunobiology</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>filariasis</term></item><item><term>Brugia malayi</term></item><item><term>IgG4</term></item><item><term>enzyme-linked immunosorbent assay</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1995-11-10">Modified</change><change when="1996">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/A6451A7D37F50594887F718B6EA74C3CB9DB73BA/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="sco"><item-info><jid>TRSTMH</jid><aid>96901406</aid><ce:pii>S0035-9203(96)90140-6</ce:pii><ce:doi>10.1016/S0035-9203(96)90140-6</ce:doi><ce:copyright type="unknown" year="1996"></ce:copyright><ce:doctopics><ce:doctopic><ce:text>Immunobiology</ce:text></ce:doctopic></ce:doctopics></item-info><head><ce:title>A filter paper technique for the detection of anti-filarial IgG<ce:inf>4</ce:inf> in lymphatic filariasis</ce:title><ce:author-group><ce:author><ce:given-name>Annemarie J.</ce:given-name><ce:surname>Terhell</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Melang</ce:given-name><ce:surname>Haarbrink</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Kunar</ce:given-name><ce:surname>Abadi</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Daniëlle C.H.</ce:given-name><ce:surname>Bronneberg</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Marjo C.</ce:given-name><ce:surname>Tieleman</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Muhammad</ce:given-name><ce:surname>Asri</ce:surname><ce:cross-ref refid="AFF3"><ce:sup>3</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Maria</ce:given-name><ce:surname>Yazdanbakhsh</ce:surname><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref><ce:e-address>maria@rullf2.leidenuniv.nl</ce:e-address></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Parasitology, Leiden University, The Netherlands</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Department of Parasitology, Hasanuddin University, Ujung Pandang, Indonesia</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>c</ce:label><ce:textfn>Communicable Disease Centre (CDC), Ujung Pandang, Indonesia</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Address for correspondence: Dr Maria Yazdanbakhsh, Department of Parasitology, Leiden University, Wassenaarseweg 62, Postbus 9605, 2300 RC Leiden, The Netherlands; fax +31 71 5276850.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="12" month="10" year="1995"></ce:date-received><ce:date-revised day="10" month="11" year="1995"></ce:date-revised><ce:date-accepted day="14" month="11" year="1995"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>In a previous study performed in south Sulawesi (Sulawesi Selatan), Indonesia, we established that the immunoglobulin 64 (IgG<ce:inf>4</ce:inf>) enzyme-linked immunosorbent assay (<ce:small-caps>elisa</ce:small-caps>) is a suitable community diagnostic method and that it can distinguish areas of high and low prevelances within short distances. In an attempt to make this diagnostic tool more applicable in the field, a comparative study using serum and blood collected on filter paper was undertaken with 568 individuals living in 2 areas with different endemicity for brugian filariasis in south Sulawesi. In Mamuju district, where the microfilaria (mf) prevalence of the studied individuals was 18·4%, antifilarial IgG<ce:inf>4</ce:inf> was present in 73·1% of the venepuncture samples and 72·5% of the filter paper samples, respectively. In Mangkutane district, where lymphatic filariasis is transmitted at a low level (mf rate 2·4%), antifilarial IgG<ce:inf>4</ce:inf> was detected in 35·5% and 39·9% of similar samples, respectively. There was no significant difference in the IgG<ce:inf>4</ce:inf> detection rate determined from venepuncture and filter paper samples from the same donors (<ce:italic>P</ce:italic> = 0·124), and the IgG<ce:inf>4</ce:inf> values were highly correlated (<ce:italic>ν</ce:italic> = 0·97, <ce:italic>P</ce:italic> < 0·001, <ce:italic>n</ce:italic> = 568). These results indicate that the filter paper technique for collection of blood samples is a suitable alternative to venepuncture for use in the IgG<ce:inf>4</ce:inf> <ce:small-caps>elisa</ce:small-caps>.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>filariasis</ce:text></ce:keyword><ce:keyword><ce:text><ce:italic>Brugia malayi</ce:italic></ce:text></ce:keyword><ce:keyword><ce:text>IgG<ce:inf>4</ce:inf></ce:text></ce:keyword><ce:keyword><ce:text>enzyme-linked immunosorbent assay</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>A filter paper technique for the detection of anti-filarial IgG4 in lymphatic filariasis</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>A filter paper technique for the detection of anti-filarial IgG</title></titleInfo><name type="personal"><namePart type="given">Annemarie J.</namePart><namePart type="family">Terhell</namePart><affiliation>Department of Parasitology, Leiden University, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Melang</namePart><namePart type="family">Haarbrink</namePart><affiliation>Department of Parasitology, Leiden University, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kunar</namePart><namePart type="family">Abadi</namePart><affiliation>Department of Parasitology, Hasanuddin University, Ujung Pandang, Indonesia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Daniëlle C.H.</namePart><namePart type="family">Bronneberg</namePart><affiliation>Department of Parasitology, Leiden University, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marjo C.</namePart><namePart type="family">Tieleman</namePart><affiliation>Department of Parasitology, Leiden University, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Muhammad</namePart><namePart type="family">Asri</namePart><affiliation>Communicable Disease Centre (CDC), Ujung Pandang, Indonesia</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Maria</namePart><namePart type="family">Yazdanbakhsh</namePart><affiliation>Address for correspondence: Dr Maria Yazdanbakhsh, Department of Parasitology, Leiden University, Wassenaarseweg 62, Postbus 9605, 2300 RC Leiden, The Netherlands; fax +31 71 5276850.</affiliation><affiliation>Department of Parasitology, Leiden University, The Netherlands</affiliation><affiliation>E-mail: maria@rullf2.leidenuniv.nl</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="Short communication"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1996</dateIssued><dateModified encoding="w3cdtf">1995-11-10</dateModified><copyrightDate encoding="w3cdtf">1996</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">In a previous study performed in south Sulawesi (Sulawesi Selatan), Indonesia, we established that the immunoglobulin 64 (IgG4) enzyme-linked immunosorbent assay (elisa) is a suitable community diagnostic method and that it can distinguish areas of high and low prevelances within short distances. In an attempt to make this diagnostic tool more applicable in the field, a comparative study using serum and blood collected on filter paper was undertaken with 568 individuals living in 2 areas with different endemicity for brugian filariasis in south Sulawesi. In Mamuju district, where the microfilaria (mf) prevalence of the studied individuals was 18·4%, antifilarial IgG4 was present in 73·1% of the venepuncture samples and 72·5% of the filter paper samples, respectively. In Mangkutane district, where lymphatic filariasis is transmitted at a low level (mf rate 2·4%), antifilarial IgG4 was detected in 35·5% and 39·9% of similar samples, respectively. There was no significant difference in the IgG4 detection rate determined from venepuncture and filter paper samples from the same donors (P = 0·124), and the IgG4 values were highly correlated (ν = 0·97, P < 0·001, n = 568). These results indicate that the filter paper technique for collection of blood samples is a suitable alternative to venepuncture for use in the IgG4 elisa.</abstract><subject><genre>article-category</genre><topic>Immunobiology</topic></subject><subject><genre>Keywords</genre><topic>filariasis</topic><topic>Brugia malayi</topic><topic>IgG4</topic><topic>enzyme-linked immunosorbent assay</topic></subject><relatedItem type="host"><titleInfo><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title></titleInfo><titleInfo type="abbreviated"><title>TRSTMH</title></titleInfo><genre type="journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">199603</dateIssued></originInfo><identifier type="ISSN">0035-9203</identifier><identifier type="PII">S0035-9203(00)X0178-2</identifier><part><date>199603</date><detail type="volume"><number>90</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue pages"><start>97</start><end>208</end></extent><extent unit="pages"><start>196</start><end>198</end></extent></part></relatedItem><identifier type="istex">A6451A7D37F50594887F718B6EA74C3CB9DB73BA</identifier><identifier type="DOI">10.1016/S0035-9203(96)90140-6</identifier><identifier type="PII">S0035-9203(96)90140-6</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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