Cellular immune response studies in bancroftian filariasis
Identifieur interne : 003F58 ( Istex/Corpus ); précédent : 003F57; suivant : 003F59Cellular immune response studies in bancroftian filariasis
Auteurs : J. Regunathan ; K. Jayaraman ; P. KalirajSource :
- Journal of Helminthology [ 0022-149X ] ; 1997-09.
Abstract
An attempt was made to identify the filarial specific antigens that are capable of inducing immune response in human filariasis. Lymphocytes were taken from three clinically defined groups living in an endemic area in Madras, namely microfilaraemic (MF) subjects with microfilariae in their blood smear without any clinical symptoms, chronic pathology (CP) individuals with lymphangitis or lymphadenitis in combination with a history of recurrent filarial fevers or lymphoedema, and endemic normals (EN) subjects without microfilariae nor any clinical symptoms of pathology. Lymphocytes from the three groups responded with no significant difference (P = 0.21) in their proliferative index to PPD and PHA, although lymphocytes from MF individuals showed significantly (P < 0.001) less proliferative index to Brugia malayi antigen (BMA) than the CP and EN subjects. This antigen specific cellular unresponsiveness seen in MF patients was not reversed by the addition of recombinant IL-lα, IL-1β, and IFN-γ, but the addition of sera from EN individuals seemed to restore this unresponsiveness (P < 0.001). The peripheral blood mononuclear cells from MF patients secreted more IL-1 in response to BMA induction than the same from CP and EN individuals. A 58 kDa recombinant protein isolated from a Wuchereria bancrofti genomic library (58 kDa) had mounted a higher proliferative response to lymphocytes from all three groups compared to BMA (P < 0.001) indicating the possible use of recombinant filarial protein to mount immunological responses in filarial patients.
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DOI: 10.1017/S0022149X00016047
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Kaliraj</name><affiliation><mods:affiliation>Centre for Biotechnology, Anna University, Madras 600 025, India</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:86D7B67D946D45BEC660C5BFBBCAAFD510A6106D</idno><date when="1997" year="1997">1997</date><idno type="doi">10.1017/S0022149X00016047</idno><idno type="url">https://api.istex.fr/document/86D7B67D946D45BEC660C5BFBBCAAFD510A6106D/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">003F58</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">003F58</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Cellular immune response studies in bancroftian filariasis</title><author><name sortKey="Regunathan, J" sort="Regunathan, J" uniqKey="Regunathan J" first="J." last="Regunathan">J. Regunathan</name><affiliation><mods:affiliation>Centre for Biotechnology, Anna University, Madras 600 025, India</mods:affiliation></affiliation></author><author><name sortKey="Jayaraman, K" sort="Jayaraman, K" uniqKey="Jayaraman K" first="K." last="Jayaraman">K. Jayaraman</name><affiliation><mods:affiliation>Centre for Biotechnology, Anna University, Madras 600 025, India</mods:affiliation></affiliation></author><author><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P." last="Kaliraj">P. Kaliraj</name><affiliation><mods:affiliation>Centre for Biotechnology, Anna University, Madras 600 025, India</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Helminthology</title><title level="j" type="abbrev">J. Helminthol.</title><idno type="ISSN">0022-149X</idno><idno type="eISSN">1475-2697</idno><imprint><publisher>Cambridge University Press</publisher><pubPlace>Cambridge, UK</pubPlace><date type="published" when="1997-09">1997-09</date><biblScope unit="volume">71</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="265">265</biblScope><biblScope unit="page" to="267">267</biblScope></imprint><idno type="ISSN">0022-149X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-149X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">An attempt was made to identify the filarial specific antigens that are capable of inducing immune response in human filariasis. Lymphocytes were taken from three clinically defined groups living in an endemic area in Madras, namely microfilaraemic (MF) subjects with microfilariae in their blood smear without any clinical symptoms, chronic pathology (CP) individuals with lymphangitis or lymphadenitis in combination with a history of recurrent filarial fevers or lymphoedema, and endemic normals (EN) subjects without microfilariae nor any clinical symptoms of pathology. Lymphocytes from the three groups responded with no significant difference (P = 0.21) in their proliferative index to PPD and PHA, although lymphocytes from MF individuals showed significantly (P < 0.001) less proliferative index to Brugia malayi antigen (BMA) than the CP and EN subjects. This antigen specific cellular unresponsiveness seen in MF patients was not reversed by the addition of recombinant IL-lα, IL-1β, and IFN-γ, but the addition of sera from EN individuals seemed to restore this unresponsiveness (P < 0.001). The peripheral blood mononuclear cells from MF patients secreted more IL-1 in response to BMA induction than the same from CP and EN individuals. A 58 kDa recombinant protein isolated from a Wuchereria bancrofti genomic library (58 kDa) had mounted a higher proliferative response to lymphocytes from all three groups compared to BMA (P < 0.001) indicating the possible use of recombinant filarial protein to mount immunological responses in filarial patients.</div></front></TEI><istex><corpusName>cambridge</corpusName><author><json:item><name>J. Regunathan</name><affiliations><json:string>Centre for Biotechnology, Anna University, Madras 600 025, India</json:string></affiliations></json:item><json:item><name>K. Jayaraman</name><affiliations><json:string>Centre for Biotechnology, Anna University, Madras 600 025, India</json:string></affiliations></json:item><json:item><name>P. Kaliraj</name><affiliations><json:string>Centre for Biotechnology, Anna University, Madras 600 025, India</json:string></affiliations></json:item></author><articleId><json:string>01604</json:string></articleId><arkIstex>ark:/67375/6GQ-WL0N9JHS-T</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>brief-report</json:string></originalGenre><abstract>An attempt was made to identify the filarial specific antigens that are capable of inducing immune response in human filariasis. Lymphocytes were taken from three clinically defined groups living in an endemic area in Madras, namely microfilaraemic (MF) subjects with microfilariae in their blood smear without any clinical symptoms, chronic pathology (CP) individuals with lymphangitis or lymphadenitis in combination with a history of recurrent filarial fevers or lymphoedema, and endemic normals (EN) subjects without microfilariae nor any clinical symptoms of pathology. Lymphocytes from the three groups responded with no significant difference (P = 0.21) in their proliferative index to PPD and PHA, although lymphocytes from MF individuals showed significantly (P > 0.001) less proliferative index to Brugia malayi antigen (BMA) than the CP and EN subjects. This antigen specific cellular unresponsiveness seen in MF patients was not reversed by the addition of recombinant IL-lα, IL-1β, and IFN-γ, but the addition of sera from EN individuals seemed to restore this unresponsiveness (P > 0.001). The peripheral blood mononuclear cells from MF patients secreted more IL-1 in response to BMA induction than the same from CP and EN individuals. A 58 kDa recombinant protein isolated from a Wuchereria bancrofti genomic library (58 kDa) had mounted a higher proliferative response to lymphocytes from all three groups compared to BMA (P > 0.001) indicating the possible use of recombinant filarial protein to mount immunological responses in filarial patients.</abstract><qualityIndicators><score>6.565</score><pdfWordCount>1757</pdfWordCount><pdfCharCount>10931</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>3</pdfPageCount><pdfPageSize>595.44 x 841.68 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>234</abstractWordCount><abstractCharCount>1564</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Cellular immune response studies in bancroftian filariasis</title><pii><json:string>S0022149X00016047</json:string></pii><genre><json:string>brief-communication</json:string></genre><host><title>Journal of Helminthology</title><language><json:string>unknown</json:string></language><issn><json:string>0022-149X</json:string></issn><eissn><json:string>1475-2697</json:string></eissn><publisherId><json:string>JHL</json:string></publisherId><volume>71</volume><issue>3</issue><pages><first>265</first><last>267</last><total>3</total></pages><genre><json:string>journal</json:string></genre></host><ark><json:string>ark:/67375/6GQ-WL0N9JHS-T</json:string></ark><publicationDate>1997</publicationDate><copyrightDate>1997</copyrightDate><doi><json:string>10.1017/S0022149X00016047</json:string></doi><id>86D7B67D946D45BEC660C5BFBBCAAFD510A6106D</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/86D7B67D946D45BEC660C5BFBBCAAFD510A6106D/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/86D7B67D946D45BEC660C5BFBBCAAFD510A6106D/fulltext/zip</uri></json:item><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/86D7B67D946D45BEC660C5BFBBCAAFD510A6106D/fulltext/txt</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/86D7B67D946D45BEC660C5BFBBCAAFD510A6106D/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Cellular immune response studies in bancroftian filariasis</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://publisher-list.data.istex.fr">Cambridge University Press</publisher><pubPlace>Cambridge, UK</pubPlace><availability><licence><p>Copyright © Cambridge University Press 1997</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-G3RCRD03-V">cambridge</p></availability><date>1997</date></publicationStmt><notesStmt><note type="brief-communication" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note>*Author for correspondence.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Cellular immune response studies in bancroftian filariasis</title><author xml:id="author-0000"><persName><forename type="first">J.</forename><surname>Regunathan</surname></persName><affiliation>Centre for Biotechnology, Anna University, Madras 600 025, India</affiliation></author><author xml:id="author-0001"><persName><forename type="first">K.</forename><surname>Jayaraman</surname></persName><affiliation>Centre for Biotechnology, Anna University, Madras 600 025, India</affiliation></author><author xml:id="author-0002"><persName><forename type="first">P.</forename><surname>Kaliraj</surname></persName><affiliation>Centre for Biotechnology, Anna University, Madras 600 025, India</affiliation></author><idno type="istex">86D7B67D946D45BEC660C5BFBBCAAFD510A6106D</idno><idno type="ark">ark:/67375/6GQ-WL0N9JHS-T</idno><idno type="DOI">10.1017/S0022149X00016047</idno><idno type="PII">S0022149X00016047</idno><idno type="article-id">01604</idno></analytic><monogr><title level="j">Journal of Helminthology</title><title level="j" type="abbrev">J. Helminthol.</title><idno type="pISSN">0022-149X</idno><idno type="eISSN">1475-2697</idno><idno type="publisher-id">JHL</idno><imprint><publisher>Cambridge University Press</publisher><pubPlace>Cambridge, UK</pubPlace><date type="published" when="1997-09"></date><biblScope unit="volume">71</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="265">265</biblScope><biblScope unit="page" to="267">267</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1997</date></creation><langUsage><language ident="en">en</language></langUsage><abstract style="normal"><p>An attempt was made to identify the filarial specific antigens that are capable of inducing immune response in human filariasis. Lymphocytes were taken from three clinically defined groups living in an endemic area in Madras, namely microfilaraemic (MF) subjects with microfilariae in their blood smear without any clinical symptoms, chronic pathology (CP) individuals with lymphangitis or lymphadenitis in combination with a history of recurrent filarial fevers or lymphoedema, and endemic normals (EN) subjects without microfilariae nor any clinical symptoms of pathology. Lymphocytes from the three groups responded with no significant difference (P = 0.21) in their proliferative index to PPD and PHA, although lymphocytes from MF individuals showed significantly (P < 0.001) less proliferative index to Brugia malayi antigen (BMA) than the CP and EN subjects. This antigen specific cellular unresponsiveness seen in MF patients was not reversed by the addition of recombinant IL-lα, IL-1β, and IFN-γ, but the addition of sera from EN individuals seemed to restore this unresponsiveness (P < 0.001). The peripheral blood mononuclear cells from MF patients secreted more IL-1 in response to BMA induction than the same from CP and EN individuals. A 58 kDa recombinant protein isolated from a Wuchereria bancrofti genomic library (58 kDa) had mounted a higher proliferative response to lymphocytes from all three groups compared to BMA (P < 0.001) indicating the possible use of recombinant filarial protein to mount immunological responses in filarial patients.</p></abstract></profileDesc><revisionDesc><change when="1997-09">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="corpus cambridge not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="US-ASCII"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.2 20060430//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article dtd-version="2.2" article-type="brief-report"><front><journal-meta><journal-id journal-id-type="publisher-id">JHL</journal-id><journal-title>Journal of Helminthology</journal-title><abbrev-journal-title>J. Helminthol.</abbrev-journal-title><issn pub-type="ppub">0022-149X</issn><issn pub-type="epub">1475-2697</issn><publisher><publisher-name>Cambridge University Press</publisher-name><publisher-loc>Cambridge, UK</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1017/S0022149X00016047</article-id><article-id pub-id-type="pii">S0022149X00016047</article-id><article-id pub-id-type="publisher-id">01604</article-id><title-group><article-title>Cellular immune response studies in bancroftian filariasis</article-title><alt-title alt-title-type="left-running">J. Regunathan <italic>et al.</italic></alt-title><alt-title alt-title-type="right-running">Immune response studies in filariasis</alt-title></title-group><contrib-group><contrib><name><surname>Regunathan</surname><given-names>J.</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib><name><surname>Jayaraman</surname><given-names>K.</given-names></name><xref ref-type="aff" rid="aff1"></xref></contrib><contrib><name><surname>Kaliraj</surname><given-names>P.</given-names></name><xref ref-type="aff" rid="aff1"></xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1"><addr-line>Centre for Biotechnology</addr-line>, <institution>Anna University</institution>, <addr-line>Madras 600 025</addr-line>, <country>India</country></aff><author-notes><corresp id="cor1"><label>*</label>Author for correspondence.</corresp></author-notes><pub-date pub-type="ppub"><month>09</month><year>1997</year></pub-date><volume>71</volume><issue>3</issue><fpage seq="18">265</fpage><lpage>267</lpage><history><date date-type="accepted"><day>04</day><month>09</month><year>1996</year></date></history><permissions><copyright-statement>Copyright © Cambridge University Press 1997</copyright-statement><copyright-year>1997</copyright-year><copyright-holder>Cambridge University Press</copyright-holder></permissions><abstract abstract-type="normal"><title>Abstract</title><p>An attempt was made to identify the filarial specific antigens that are capable of inducing immune response in human filariasis. Lymphocytes were taken from three clinically defined groups living in an endemic area in Madras, namely microfilaraemic (MF) subjects with microfilariae in their blood smear without any clinical symptoms, chronic pathology (CP) individuals with lymphangitis or lymphadenitis in combination with a history of recurrent filarial fevers or lymphoedema, and endemic normals (EN) subjects without microfilariae nor any clinical symptoms of pathology. Lymphocytes from the three groups responded with no significant difference (<italic>P</italic> = 0.21) in their proliferative index to PPD and PHA, although lymphocytes from MF individuals showed significantly (<italic>P</italic> < 0.001) less proliferative index to <italic>Brugia malayi</italic> antigen (BMA) than the CP and EN subjects. This antigen specific cellular unresponsiveness seen in MF patients was not reversed by the addition of recombinant IL-lα, IL-1β, and IFN-γ, but the addition of sera from EN individuals seemed to restore this unresponsiveness (<italic>P</italic> < 0.001). The peripheral blood mononuclear cells from MF patients secreted more IL-1 in response to BMA induction than the same from CP and EN individuals. A 58 kDa recombinant protein isolated from a <italic>Wuchereria bancrofti</italic> genomic library (58 kDa) had mounted a higher proliferative response to lymphocytes from all three groups compared to BMA (<italic>P</italic> < 0.001) indicating the possible use of recombinant filarial protein to mount immunological responses in filarial patients.</p></abstract><counts><page-count count="3"></page-count></counts><custom-meta-wrap><custom-meta><meta-name>pdf</meta-name><meta-value>S0022149X00016047a.pdf</meta-value></custom-meta><custom-meta><meta-name>dispart</meta-name><meta-value>Research Note</meta-value></custom-meta></custom-meta-wrap></article-meta></front><back><ref-list><title>References</title><ref><citation id="ref001" citation-type="journal"><name><surname>Abou-Zeid</surname><given-names>C.</given-names></name>, <name><surname>Filley</surname><given-names>E.</given-names></name>, <name><surname>Steele</surname><given-names>J.</given-names></name> & 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Regunathan et al.</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Cellular immune response studies in bancroftian filariasis</title></titleInfo><name type="personal"><namePart type="given">J.</namePart><namePart type="family">Regunathan</namePart><affiliation>Centre for Biotechnology, Anna University, Madras 600 025, India</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Jayaraman</namePart><affiliation>Centre for Biotechnology, Anna University, Madras 600 025, India</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Kaliraj</namePart><affiliation>Centre for Biotechnology, Anna University, Madras 600 025, India</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="brief-communication" displayLabel="brief-report" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-S9SX2MFS-0">brief-communication</genre><originInfo><publisher>Cambridge University Press</publisher><place><placeTerm type="text">Cambridge, UK</placeTerm></place><dateIssued encoding="w3cdtf">1997-09</dateIssued><copyrightDate encoding="w3cdtf">1997</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract type="normal">An attempt was made to identify the filarial specific antigens that are capable of inducing immune response in human filariasis. Lymphocytes were taken from three clinically defined groups living in an endemic area in Madras, namely microfilaraemic (MF) subjects with microfilariae in their blood smear without any clinical symptoms, chronic pathology (CP) individuals with lymphangitis or lymphadenitis in combination with a history of recurrent filarial fevers or lymphoedema, and endemic normals (EN) subjects without microfilariae nor any clinical symptoms of pathology. Lymphocytes from the three groups responded with no significant difference (P = 0.21) in their proliferative index to PPD and PHA, although lymphocytes from MF individuals showed significantly (P < 0.001) less proliferative index to Brugia malayi antigen (BMA) than the CP and EN subjects. This antigen specific cellular unresponsiveness seen in MF patients was not reversed by the addition of recombinant IL-lα, IL-1β, and IFN-γ, but the addition of sera from EN individuals seemed to restore this unresponsiveness (P < 0.001). The peripheral blood mononuclear cells from MF patients secreted more IL-1 in response to BMA induction than the same from CP and EN individuals. A 58 kDa recombinant protein isolated from a Wuchereria bancrofti genomic library (58 kDa) had mounted a higher proliferative response to lymphocytes from all three groups compared to BMA (P < 0.001) indicating the possible use of recombinant filarial protein to mount immunological responses in filarial patients.</abstract><note type="author-notes">*Author for correspondence.</note><relatedItem type="host"><titleInfo><title>Journal of Helminthology</title></titleInfo><titleInfo type="abbreviated"><title>J. Helminthol.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><identifier type="ISSN">0022-149X</identifier><identifier type="eISSN">1475-2697</identifier><identifier type="PublisherID">JHL</identifier><part><date>1997</date><detail type="volume"><caption>vol.</caption><number>71</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>265</start><end>267</end><total>3</total></extent></part></relatedItem><identifier type="istex">86D7B67D946D45BEC660C5BFBBCAAFD510A6106D</identifier><identifier type="ark">ark:/67375/6GQ-WL0N9JHS-T</identifier><identifier type="DOI">10.1017/S0022149X00016047</identifier><identifier type="PII">S0022149X00016047</identifier><identifier type="ArticleID">01604</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © Cambridge University Press 1997</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-G3RCRD03-V">cambridge</recordContentSource><recordOrigin>Copyright © Cambridge University Press 1997</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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