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P‐83 Intradermal testing in cattle: evaluation of sensitization to Dermatophagoides extracts in healthy cattle and cattle infected with Psoroptes or Chorioptes

Identifieur interne : 003506 ( Istex/Corpus ); précédent : 003505; suivant : 003507

P‐83 Intradermal testing in cattle: evaluation of sensitization to Dermatophagoides extracts in healthy cattle and cattle infected with Psoroptes or Chorioptes

Auteurs : P. Bourdeau ; C. Rivat

Source :

RBID : ISTEX:71A4EC4CE0E9CE881AC7541C306C0835C5080720

Abstract

Information on sensitization of cattle to common allergens like Pyroglyphids or approved practical methods to evaluate in vivo sensitization are lacking. The aim of the study was to evaluate both intradermal testing (IDT) and sensitization to Dermatophagoides in healthy cattle and those infested with Psororptes or Chorioptes. Animals were included in three groups: Group A, healthy (n = 50), Group B, Psoroptes ovis infestation (n = 50), and Group C, Chorioptes bovis infestation (n = 12). Two methods of IDT were compared: classical use of 1 mL insulin syringe and a tuberculination device. Reactions to histamine 1/10,000 were compared to a negative control and commercial allergens (Dermatophagoides farinae, D. pteronyssinus) by injections (0.1 mL) in the midsection of the lateral neck. Readings were made at 30 min (diameter of the wheal and thickening of the skin fold with a calliper square) and at 48 h. The best results were obtained combining the tuberculin device and diameter of the wheal (100% correlation with the calliper square). Reactions to histamine were significantly more intense than for the allergens tested. Positive intradermal reactions included: Group A, D. farinae 42% and D. pteronyssinus 36%; Group B, D. farinae 74% and D. pteronyssinus 50%; Group C, D. farinae 58% and D. pteronyssinus 42%. No delayed reaction was detected. The possible use of this cross‐reactivity for a rapid evaluation of infestation of herds by parasitic mites needs more evaluation. Funding: Self‐funded.

Url:
DOI: 10.1111/j.1365-3164.2004.00414_83.x

Links to Exploration step

ISTEX:71A4EC4CE0E9CE881AC7541C306C0835C5080720

Le document en format XML

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Intradermal testing in cattle: evaluation of sensitization to
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<title level="j" type="main">Veterinary Dermatology</title>
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<div type="abstract" xml:lang="en">Information on sensitization of cattle to common allergens like Pyroglyphids or approved practical methods to evaluate in vivo sensitization are lacking. The aim of the study was to evaluate both intradermal testing (IDT) and sensitization to Dermatophagoides in healthy cattle and those infested with Psororptes or Chorioptes. Animals were included in three groups: Group A, healthy (n = 50), Group B, Psoroptes ovis infestation (n = 50), and Group C, Chorioptes bovis infestation (n = 12). Two methods of IDT were compared: classical use of 1 mL insulin syringe and a tuberculination device. Reactions to histamine 1/10,000 were compared to a negative control and commercial allergens (Dermatophagoides farinae, D. pteronyssinus) by injections (0.1 mL) in the midsection of the lateral neck. Readings were made at 30 min (diameter of the wheal and thickening of the skin fold with a calliper square) and at 48 h. The best results were obtained combining the tuberculin device and diameter of the wheal (100% correlation with the calliper square). Reactions to histamine were significantly more intense than for the allergens tested. Positive intradermal reactions included: Group A, D. farinae 42% and D. pteronyssinus 36%; Group B, D. farinae 74% and D. pteronyssinus 50%; Group C, D. farinae 58% and D. pteronyssinus 42%. No delayed reaction was detected. The possible use of this cross‐reactivity for a rapid evaluation of infestation of herds by parasitic mites needs more evaluation. Funding: Self‐funded.</div>
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